Reviewed, UniProtKB/Swiss-Prot Q9NPF4 (OSGEP_HUMAN)

Names and origin

Protein names

Recommended name:
    Probable O-sialoglycoprotein endopeptidase
      Short name=hOSGEP
    EC=3.4.24.57

Gene names

Name:	OSGEP
Synonyms:	GCPL1

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	335 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	O-sialoglycoprotein endopeptidases specifically cleave the polypeptide backbone of membrane glycoproteins that contain clusters of O-linked sialoglycans By similarity.
Catalytic activity	Hydrolysis of O-sialoglycoproteins; cleaves 31-Arg-|-Asp-32 bond in glycophorin A. Does not cleave unglycosylated proteins, desialylated glycoproteins or glycoproteins that are only N-glycosylated.
Cofactor	Zinc Probable.
Tissue specificity	Widely expressed at low level. Expressed in heart, placenta, liver, kidney, lung, brain, skeletal muscle and pancreas. Ref.1
Sequence similarities	Belongs to the peptidase M22 family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
DNAJA1	P31689	1	EBI-1056510,EBI-347834
LAGE3	Q14657	1	EBI-1056510,EBI-1052105
RAB5C	P51148	1	EBI-1056510,EBI-1054923

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 335	335	Probable O-sialoglycoprotein endopeptidase		PRO_0000096984
Sites
	Metal binding	109	1	Zinc Potential
	Metal binding	113	1	Zinc Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9NPF4-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 54DCBBB26C03E8FB	FASTA	335	36,427
	10 20 30 40 50 60 MPAVLGFEGS ANKIGVGVVR DGKVLANPRR TYVTPPGTGF LPGDTARHHR AVILDLLQEA 70 80 90 100 110 120 LTESGLTSQD IDCIAYTKGP GMGAPLVSVA VVARTVAQLW NKPLVGVNHC IGHIEMGRLI 130 140 150 160 170 180 TGATSPTVLY VSGGNTQVIA YSEHRYRIFG ETIDIAVGNC LDRFARVLKI SNDPSPGYNI 190 200 210 220 230 240 EQMAKRGKKL VELPYTVKGM DVSFSGILSF IEDVAHRMLA TGECTPEDLC FSLQETVFAM 250 260 270 280 290 300 LVEITERAMA HCGSQEALIV GGVGCNVRLQ EMMATMCQER GARLFATDER FCIDNGAMIA 310 320 330 QAGWEMFRAG HRTPLSDSGV TQRYRTDEVE VTWRD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing analysis of a putative human O-sialoglycoprotein endopeptidase gene (OSGEP) and analysis of a bidirectional promoter between the OSGEP and APEX genes." Seki Y., Ikeda S., Kiyohara H., Ayabe H., Seki T., Matsui H. Gene 285:101-108(2002) [PubMed: 12039036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], TISSUE SPECIFICITY. Tissue: Placenta.
[2]	"Cloning and sequencing of putative human sialoglycoprotease." Wigelsworth D.J., Coadwell J., Rawlings N.D., Barrett A.J. Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Carcinoma.
[4]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Blood.
[6]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB047823 Genomic DNA. Translation: BAB33147.1. AB050442 mRNA. Translation: BAB33172.1. AJ271669 mRNA. Translation: CAB71031.1. AK000418 mRNA. Translation: BAA91150.1. CR457232 mRNA. Translation: CAG33513.1. BC032310 mRNA. Translation: AAH32310.1.
IPI	IPI00015809.
RefSeq	NP_060277.1.
UniGene	Hs.525196 Hs.73722
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9NPF4. 3 interactions.
STRING	Q9NPF4.
Protein family/group databases
MEROPS	M22.003.
Proteomic databases
PeptideAtlas	Q9NPF4.
PRIDE	Q9NPF4.
Genome annotation databases
Ensembl	ENST00000206542; ENSP00000206542; ENSG00000092094; Homo sapiens. [Genome view]
GeneID	55644.
KEGG	hsa:55644.
UCSC	uc001vxf.1. human.
Organism-specific databases
CTD	55644.
GeneCards	GC14M019985.
HGNC	HGNC:18028. OSGEP.
MIM	610107. gene.
PharmGKB	PA32834.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q9NPF4.
HOVERGEN	Q9NPF4.
OMA	TQRFRTD.
Enzyme and pathway databases
BRENDA	3.4.24.57. 247.
Gene expression databases
ArrayExpress	Q9NPF4.
Bgee	Q9NPF4.
CleanEx	HS_OSGEP.
Genevestigator	Q9NPF4.
GermOnline	ENSG00000092094. Homo sapiens.
Family and domain databases
InterPro	IPR009180. Pept_M22_Osialgl. IPR000905. Peptidase_M22. IPR017860. Peptidase_M22_CS. IPR017861. Peptidase_M22_subgr. [Graphical view]
PANTHER	PTHR11735. Pept_M22_Osialgl. 1 hit.
Pfam	PF00814. Peptidase_M22. 1 hit. [Graphical view]
PRINTS	PR00789. OSIALOPTASE.
ProDom	PD002367. Peptidase_M22. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00329. gcp. 1 hit.
PROSITE	PS01016. GLYCOPROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	60334.
SOURCE	Search...

Entry information

Entry name

OSGEP_HUMAN

Accession

Primary (citable) accession number: Q9NPF4
Secondary accession number(s): Q6IAC3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 24, 2004
Last sequence update:	October 1, 2000
Last modified:	November 3, 2009
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.