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Protein

Probable tRNA N6-adenosine threonylcarbamoyltransferase

Gene

OSGEP

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. OSGEP likely plays a direct catalytic role in this reaction, but requires other protein(s) of the complex to fulfill this activity.UniRule annotation

Catalytic activityi

L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N(6)-L-threonylcarbamoyladenine(37) in tRNA.

Cofactori

a divalent metal cationUniRule annotationNote: Binds 1 divalent metal cation per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi109 – 1091Divalent metal cationUniRule annotation
Metal bindingi113 – 1131Divalent metal cationUniRule annotation
Metal bindingi130 – 1301Divalent metal cationUniRule annotation
Binding sitei162 – 1621SubstrateUniRule annotation
Binding sitei177 – 1771Substrate; via amide nitrogenUniRule annotation
Binding sitei181 – 1811SubstrateUniRule annotation
Binding sitei266 – 2661SubstrateUniRule annotation
Metal bindingi294 – 2941Divalent metal cationUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable tRNA N6-adenosine threonylcarbamoyltransferaseUniRule annotation (EC:2.3.1.234UniRule annotation)
Alternative name(s):
N6-L-threonylcarbamoyladenine synthase
Short name:
t(6)A synthase
O-sialoglycoprotein endopeptidaseUniRule annotation
Short name:
hOSGEP
t(6)A37 threonylcarbamoyladenosine biosynthesis protein OSGEPUniRule annotation
tRNA threonylcarbamoyladenosine biosynthesis protein OSGEPUniRule annotation
Gene namesi
Name:OSGEPUniRule annotation
Synonyms:GCPL1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 14

Organism-specific databases

HGNCiHGNC:18028. OSGEP.

Subcellular locationi

  • Cytoplasm UniRule annotation
  • Nucleus UniRule annotation1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA32834.

Polymorphism and mutation databases

BioMutaiOSGEP.
DMDMi47605574.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 335335Probable tRNA N6-adenosine threonylcarbamoyltransferasePRO_0000096984Add
BLAST

Proteomic databases

MaxQBiQ9NPF4.
PaxDbiQ9NPF4.
PeptideAtlasiQ9NPF4.
PRIDEiQ9NPF4.

PTM databases

PhosphoSiteiQ9NPF4.

Expressioni

Tissue specificityi

Widely expressed at low level. Expressed in heart, placenta, liver, kidney, lung, brain, skeletal muscle and pancreas.1 Publication

Gene expression databases

BgeeiQ9NPF4.
CleanExiHS_OSGEP.
ExpressionAtlasiQ9NPF4. baseline and differential.
GenevisibleiQ9NPF4. HS.

Organism-specific databases

HPAiHPA039751.

Interactioni

Subunit structurei

Component of the EKC/KEOPS complex composed of at least TP53RK, TPRKB, OSGEP and LAGE3; the whole complex dimerizes. Interacts with PRAME.UniRule annotation1 Publication

Protein-protein interaction databases

BioGridi120779. 61 interactions.
IntActiQ9NPF4. 21 interactions.
MINTiMINT-3071750.
STRINGi9606.ENSP00000206542.

Structurei

3D structure databases

ProteinModelPortaliQ9NPF4.
SMRiQ9NPF4. Positions 5-333.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni130 – 1345Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the KAE1 / TsaD family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0533.
GeneTreeiENSGT00550000074933.
HOGENOMiHOG000109569.
HOVERGENiHBG051713.
InParanoidiQ9NPF4.
KOiK01409.
OMAiERYCIDN.
PhylomeDBiQ9NPF4.
TreeFamiTF313621.

Family and domain databases

HAMAPiMF_01446. Kae1_arch.
InterProiIPR000905. Gcp-like_dom.
IPR017861. KAE1/TsaD.
IPR017860. Peptidase_M22_CS.
[Graphical view]
PfamiPF00814. Peptidase_M22. 1 hit.
[Graphical view]
PRINTSiPR00789. OSIALOPTASE.
TIGRFAMsiTIGR00329. gcp_kae1. 1 hit.
PROSITEiPS01016. GLYCOPROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9NPF4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPAVLGFEGS ANKIGVGVVR DGKVLANPRR TYVTPPGTGF LPGDTARHHR
60 70 80 90 100
AVILDLLQEA LTESGLTSQD IDCIAYTKGP GMGAPLVSVA VVARTVAQLW
110 120 130 140 150
NKPLVGVNHC IGHIEMGRLI TGATSPTVLY VSGGNTQVIA YSEHRYRIFG
160 170 180 190 200
ETIDIAVGNC LDRFARVLKI SNDPSPGYNI EQMAKRGKKL VELPYTVKGM
210 220 230 240 250
DVSFSGILSF IEDVAHRMLA TGECTPEDLC FSLQETVFAM LVEITERAMA
260 270 280 290 300
HCGSQEALIV GGVGCNVRLQ EMMATMCQER GARLFATDER FCIDNGAMIA
310 320 330
QAGWEMFRAG HRTPLSDSGV TQRYRTDEVE VTWRD
Length:335
Mass (Da):36,427
Last modified:October 1, 2000 - v1
Checksum:i54DCBBB26C03E8FB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB047823 Genomic DNA. Translation: BAB33147.1.
AB050442 mRNA. Translation: BAB33172.1.
AJ271669 mRNA. Translation: CAB71031.1.
AK000418 mRNA. Translation: BAA91150.1.
CR457232 mRNA. Translation: CAG33513.1.
BC032310 mRNA. Translation: AAH32310.1.
CCDSiCCDS9549.1.
RefSeqiNP_060277.1. NM_017807.3.
UniGeneiHs.525196.

Genome annotation databases

EnsembliENST00000206542; ENSP00000206542; ENSG00000092094.
GeneIDi55644.
KEGGihsa:55644.
UCSCiuc001vxf.3. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB047823 Genomic DNA. Translation: BAB33147.1.
AB050442 mRNA. Translation: BAB33172.1.
AJ271669 mRNA. Translation: CAB71031.1.
AK000418 mRNA. Translation: BAA91150.1.
CR457232 mRNA. Translation: CAG33513.1.
BC032310 mRNA. Translation: AAH32310.1.
CCDSiCCDS9549.1.
RefSeqiNP_060277.1. NM_017807.3.
UniGeneiHs.525196.

3D structure databases

ProteinModelPortaliQ9NPF4.
SMRiQ9NPF4. Positions 5-333.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120779. 61 interactions.
IntActiQ9NPF4. 21 interactions.
MINTiMINT-3071750.
STRINGi9606.ENSP00000206542.

PTM databases

PhosphoSiteiQ9NPF4.

Polymorphism and mutation databases

BioMutaiOSGEP.
DMDMi47605574.

Proteomic databases

MaxQBiQ9NPF4.
PaxDbiQ9NPF4.
PeptideAtlasiQ9NPF4.
PRIDEiQ9NPF4.

Protocols and materials databases

DNASUi55644.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000206542; ENSP00000206542; ENSG00000092094.
GeneIDi55644.
KEGGihsa:55644.
UCSCiuc001vxf.3. human.

Organism-specific databases

CTDi55644.
GeneCardsiGC14M020915.
HGNCiHGNC:18028. OSGEP.
HPAiHPA039751.
MIMi610107. gene.
neXtProtiNX_Q9NPF4.
PharmGKBiPA32834.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG0533.
GeneTreeiENSGT00550000074933.
HOGENOMiHOG000109569.
HOVERGENiHBG051713.
InParanoidiQ9NPF4.
KOiK01409.
OMAiERYCIDN.
PhylomeDBiQ9NPF4.
TreeFamiTF313621.

Miscellaneous databases

ChiTaRSiOSGEP. human.
GeneWikiiOSGEP.
GenomeRNAii55644.
NextBioi60334.
PROiQ9NPF4.
SOURCEiSearch...

Gene expression databases

BgeeiQ9NPF4.
CleanExiHS_OSGEP.
ExpressionAtlasiQ9NPF4. baseline and differential.
GenevisibleiQ9NPF4. HS.

Family and domain databases

HAMAPiMF_01446. Kae1_arch.
InterProiIPR000905. Gcp-like_dom.
IPR017861. KAE1/TsaD.
IPR017860. Peptidase_M22_CS.
[Graphical view]
PfamiPF00814. Peptidase_M22. 1 hit.
[Graphical view]
PRINTSiPR00789. OSIALOPTASE.
TIGRFAMsiTIGR00329. gcp_kae1. 1 hit.
PROSITEiPS01016. GLYCOPROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequencing analysis of a putative human O-sialoglycoprotein endopeptidase gene (OSGEP) and analysis of a bidirectional promoter between the OSGEP and APEX genes."
    Seki Y., Ikeda S., Kiyohara H., Ayabe H., Seki T., Matsui H.
    Gene 285:101-108(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], TISSUE SPECIFICITY.
    Tissue: Placenta.
  2. "Cloning and sequencing of putative human sialoglycoprotease."
    Wigelsworth D.J., Coadwell J., Rawlings N.D., Barrett A.J.
    Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Carcinoma.
  4. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Blood.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "The human EKC/KEOPS complex is recruited to Cullin2 ubiquitin ligases by the human tumour antigen PRAME."
    Costessi A., Mahrour N., Sharma V., Stunnenberg R., Stoel M.A., Tijchon E., Conaway J.W., Conaway R.C., Stunnenberg H.G.
    PLoS ONE 7:E42822-E42822(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE EKC/KEOPS COMPLEX, INTERACTION WITH PRAME, SUBCELLULAR LOCATION.
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiOSGEP_HUMAN
AccessioniPrimary (citable) accession number: Q9NPF4
Secondary accession number(s): Q6IAC3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: October 1, 2000
Last modified: July 22, 2015
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.