Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Carbohydrate sulfotransferase 11

Gene

CHST11

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine (GalNAc) residue of chondroitin. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many cells and extracellular matrices. Can also sulfate Gal residues in desulfated dermatan sulfate. Preferentially sulfates in GlcA->GalNAc unit than in IdoA->GalNAc unit. Does not form 4, 6-di-O-sulfated GalNAc when chondroitin sulfate C is used as an acceptor.

Catalytic activityi

3'-phosphoadenylyl sulfate + chondroitin = adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate.2 Publications

Kineticsi

  1. KM=0.5 µM for PAPS1 Publication
  2. KM=2.1 mM for chondroitin1 Publication

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi124 – 130PAPSBy similarity7
    Nucleotide bindingi186 – 194PAPSBy similarity9

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionTransferase
    Biological processCarbohydrate metabolism

    Enzyme and pathway databases

    BioCyciMetaCyc:HS10284-MONOMER
    BRENDAi2.8.2.5 2681
    ReactomeiR-HSA-2022870 Chondroitin sulfate biosynthesis

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Carbohydrate sulfotransferase 11 (EC:2.8.2.5)
    Alternative name(s):
    Chondroitin 4-O-sulfotransferase 1
    Chondroitin 4-sulfotransferase 1
    Short name:
    C4S-1
    Short name:
    C4ST-1
    Short name:
    C4ST1
    Gene namesi
    Name:CHST11
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    Proteomesi
    • UP000005640 Componenti: Chromosome 12

    Organism-specific databases

    EuPathDBiHostDB:ENSG00000171310.10
    HGNCiHGNC:17422 CHST11
    MIMi610128 gene
    neXtProtiNX_Q9NPF2

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Topological domaini1 – 16CytoplasmicSequence analysisAdd BLAST16
    Transmembranei17 – 37Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
    Topological domaini38 – 352LumenalSequence analysisAdd BLAST315

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    Pathology & Biotechi

    Involvement in diseasei

    A chromosomal aberration involving CHST11 is found in B-cell chronic lymphocytic leukemias. Translocation t(12;14)(q23;q32) with IgH.1 Publication

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi125K → Q: Abolishes enzyme activity but does not affect stability of the protein. 1 Publication1
    Mutagenesisi205N → S: Induces a weak decrease in enzyme activity but has no effect on stability of the protein. Unstable protein; when associated with S-223 and S-321. 1 Publication1
    Mutagenesisi223N → S: Induces a weak decrease in enzyme activity but has no effect on stability of the protein. Unstable protein; when associated with S-205 and S-321. 1 Publication1
    Mutagenesisi321N → S: Induces a strong decrease in enzyme activity but has no effect on stability of the protein. Unstable protein; when associated with S-205 and S-223. 1
    Mutagenesisi342N → S: Induces a strong decrease in enzyme activity has no effect on stability of the protein. 1 Publication1

    Organism-specific databases

    DisGeNETi50515
    OpenTargetsiENSG00000171310
    PharmGKBiPA134875681

    Polymorphism and mutation databases

    BioMutaiCHST11
    DMDMi61212137

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001896641 – 352Carbohydrate sulfotransferase 11Add BLAST352

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Glycosylationi205N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi223N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi321N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi342N-linked (GlcNAc...) asparagine1 Publication1

    Post-translational modificationi

    N-glycosylated; required for activity and stability.1 Publication

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    EPDiQ9NPF2
    MaxQBiQ9NPF2
    PaxDbiQ9NPF2
    PeptideAtlasiQ9NPF2
    PRIDEiQ9NPF2
    ProteomicsDBi81982
    81983 [Q9NPF2-2]

    PTM databases

    iPTMnetiQ9NPF2
    PhosphoSitePlusiQ9NPF2
    SwissPalmiQ9NPF2

    Expressioni

    Tissue specificityi

    Widely expressed. Highly expressed in spleen, thymus, bone marrow, peripheral blood leukocytes, lymph node, heart, brain, lung and placenta.2 Publications

    Gene expression databases

    BgeeiENSG00000171310
    CleanExiHS_CHST11
    ExpressionAtlasiQ9NPF2 baseline and differential
    GenevisibleiQ9NPF2 HS

    Organism-specific databases

    HPAiHPA052828

    Interactioni

    Protein-protein interaction databases

    BioGridi119084, 5 interactors
    STRINGi9606.ENSP00000305725

    Structurei

    3D structure databases

    ProteinModelPortaliQ9NPF2
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the sulfotransferase 2 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG4651 Eukaryota
    ENOG4111GJR LUCA
    GeneTreeiENSGT00760000119214
    HOGENOMiHOG000231801
    HOVERGENiHBG050952
    InParanoidiQ9NPF2
    KOiK01017
    OMAiPLNEHWQ
    OrthoDBiEOG091G0FZO
    PhylomeDBiQ9NPF2
    TreeFamiTF325581

    Family and domain databases

    InterProiView protein in InterPro
    IPR018011 Carb_sulfotrans_8-10
    IPR005331 Sulfotransferase
    PANTHERiPTHR12137 PTHR12137, 1 hit
    PfamiView protein in Pfam
    PF03567 Sulfotransfer_2, 1 hit

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

    Isoform 1 (identifier: Q9NPF2-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MKPALLEVMR MNRICRMVLA TCLGSFILVI FYFQSMLHPV MRRNPFGVDI
    60 70 80 90 100
    CCRKGSRSPL QELYNPIQLE LSNTAVLHQM RRDQVTDTCR ANSATSRKRR
    110 120 130 140 150
    VLTPNDLKHL VVDEDHELIY CYVPKVACTN WKRLMMVLTG RGKYSDPMEI
    160 170 180 190 200
    PANEAHVSAN LKTLNQYSIP EINHRLKSYM KFLFVREPFE RLVSAYRNKF
    210 220 230 240 250
    TQKYNISFHK RYGTKIIKRQ RKNATQEALR KGDDVKFEEF VAYLIDPHTQ
    260 270 280 290 300
    REEPFNEHWQ TVYSLCHPCH IHYDLVGKYE TLEEDSNYVL QLAGVGSYLK
    310 320 330 340 350
    FPTYAKSTRT TDEMTTEFFQ NISSEHQTQL YEVYKLDFLM FNYSVPSYLK

    LE
    Length:352
    Mass (Da):41,555
    Last modified:October 1, 2000 - v1
    Checksum:i55DF1D29D6703307
    GO
    Isoform 2 (identifier: Q9NPF2-2) [UniParc]FASTAAdd to basket
    Also known as: C4S-1A

    The sequence of this isoform differs from the canonical sequence as follows:
         35-40: SMLHPV → I

    Show »
    Length:347
    Mass (Da):41,003
    Checksum:iDEB2646BC0CA5FF8
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti289V → F in CAB87380 (Ref. 3) Curated1

    Alternative sequence

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Alternative sequenceiVSP_01299235 – 40SMLHPV → I in isoform 2. 1 Publication6

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB042326 mRNA Translation: BAA95485.1
    AF239820 mRNA Translation: AAF81691.1
    AJ269537 mRNA Translation: CAB87380.1
    AJ289134 mRNA Translation: CAB92134.1
    AK290923 mRNA Translation: BAF83612.1
    CH471054 Genomic DNA Translation: EAW97748.1
    BC013315 mRNA Translation: AAH13315.1
    CCDSiCCDS55878.1 [Q9NPF2-2]
    CCDS9099.1 [Q9NPF2-1]
    PIRiJC7525
    RefSeqiNP_001167453.1, NM_001173982.1 [Q9NPF2-2]
    NP_060883.1, NM_018413.5 [Q9NPF2-1]
    UniGeneiHs.17569

    Genome annotation databases

    EnsembliENST00000303694; ENSP00000305725; ENSG00000171310 [Q9NPF2-1]
    ENST00000549260; ENSP00000450004; ENSG00000171310 [Q9NPF2-2]
    GeneIDi50515
    KEGGihsa:50515
    UCSCiuc001tky.4 human [Q9NPF2-1]

    Keywords - Coding sequence diversityi

    Alternative splicing, Chromosomal rearrangement

    Similar proteinsi

    Entry informationi

    Entry nameiCHSTB_HUMAN
    AccessioniPrimary (citable) accession number: Q9NPF2
    Secondary accession number(s): A8K4F8, Q9NXY6, Q9NY36
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2005
    Last sequence update: October 1, 2000
    Last modified: June 20, 2018
    This is version 134 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

    Do not show this banner again
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health