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Protein

Sperm-associated antigen 4 protein

Gene

SPAG4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in spermatogenesis. Required for sperm head formation but not required to establish and maintain general polarity of the sperm head. Required for anchoring and organization of the manchette. Required for targeting of SUN3 and probably SYNE1 through a probable SUN1:SYNE3 LINC complex to the nuclear envelope and involved in accurate posterior sperm head localization of the complex. May anchor SUN3 the nuclear envelope. Involved in maintenance of the nuclear envelope integrity. May assist the organization and assembly of outer dense fibers (ODFs), a specific structure of the sperm tail.By similarity

GO - Molecular functioni

  • protein anchor Source: GO_Central
  • structural molecule activity Source: ProtInc

GO - Biological processi

Keywordsi

Biological processDifferentiation, Spermatogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Sperm-associated antigen 4 protein
Alternative name(s):
Outer dense fiber-associated protein SPAG4
SUN domain-containing protein 4
Gene namesi
Name:SPAG4
Synonyms:SUN4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 20

Organism-specific databases

HGNCiHGNC:11214. SPAG4.

Subcellular locationi

  • Membrane Curated; Multi-pass membrane protein Curated
  • Cytoplasmcytoskeleton By similarity
  • Cytoplasmcytoskeletonflagellum axoneme By similarity
  • Nucleus envelope By similarity
  • Nucleus inner membrane By similarity

  • Note: In spermatids, it is localized in the transient manchette and in the axoneme of elongating spermatids and epididymal sperm (By similarity). Colocalized with SEPT12 at the nuclear periphery in round spermatids, at sperm neck in elongated spermatids and at midpiece regions in ejaculated spermatozoa (PubMed:25775403).By similarity1 Publication

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei135 – 155HelicalSequence analysisAdd BLAST21
Transmembranei166 – 186HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Keywords - Cellular componenti

Cell projection, Cilium, Cytoplasm, Cytoskeleton, Flagellum, Membrane, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi6676.
OpenTargetsiENSG00000061656.
PharmGKBiPA36050.

Polymorphism and mutation databases

BioMutaiSPAG4.
DMDMi27805726.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002189161 – 437Sperm-associated antigen 4 proteinAdd BLAST437

Proteomic databases

PaxDbiQ9NPE6.
PeptideAtlasiQ9NPE6.
PRIDEiQ9NPE6.

PTM databases

iPTMnetiQ9NPE6.
PhosphoSitePlusiQ9NPE6.

Expressioni

Tissue specificityi

Predominantly epressed in testis. Expressed in ejaculated spermatozoa (at protein level).1 Publication

Gene expression databases

BgeeiENSG00000061656.
CleanExiHS_SPAG4.
ExpressionAtlasiQ9NPE6. baseline and differential.
GenevisibleiQ9NPE6. HS.

Organism-specific databases

HPAiHPA048393.
HPA061789.

Interactioni

Subunit structurei

Homodimer. Interacts with ODF1. May associate with microtubules (By similarity). Interacts with SUN3 and SYNE1; suggesting the formation of a spermatogenesis-specific LINC complex; a SUN domain-based heterotrimer with SUN3 may associate with SYNE1 (By similarity). Interacts with SEPT12 and LMNB1; during spermatogenesis (PubMed:25775403).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
SEPT12Q8IYM16EBI-10819434,EBI-2585067

GO - Molecular functioni

Protein-protein interaction databases

BioGridi112558. 2 interactors.
IntActiQ9NPE6. 23 interactors.
STRINGi9606.ENSP00000363391.

Structurei

3D structure databases

ProteinModelPortaliQ9NPE6.
SMRiQ9NPE6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini265 – 425SUNPROSITE-ProRule annotationAdd BLAST161

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili197 – 244Sequence analysisAdd BLAST48

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2687. Eukaryota.
ENOG410YM6S. LUCA.
GeneTreeiENSGT00390000011587.
HOGENOMiHOG000246956.
HOVERGENiHBG079205.
InParanoidiQ9NPE6.
OMAiSVYREVC.
OrthoDBiEOG091G0AZ8.
PhylomeDBiQ9NPE6.
TreeFamiTF323915.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
InterProiView protein in InterPro
IPR008979. Galactose-bd-like.
IPR027776. SPAG4/SUN4.
IPR012919. SUN_dom.
PANTHERiPTHR12911:SF28. PTHR12911:SF28. 1 hit.
PfamiView protein in Pfam
PF07738. Sad1_UNC. 1 hit.
PROSITEiView protein in PROSITE
PS51469. SUN. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9NPE6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRRSSRPGSA SSSRKHTPNF FSENSSMSIT SEDSKGLRSA EPGPGEPEGR
60 70 80 90 100
RARGPSCGEP ALSAGVPGGT TWAGSSQQKP APRSHNWQTA CGAATVRGGA
110 120 130 140 150
SEPTGSPVVS EEPLDLLPTL DLRQEMPPPR VFKSFLSLLF QGLSVLLSLA
160 170 180 190 200
GDVLVSMYRE VCSIRFLFTA VSLLSLFLSA FWLGLLYLVS PLENEPKEML
210 220 230 240 250
TLSEYHERVR SQGQQLQQLQ AELDKLHKEV STVRAANSER VAKLVFQRLN
260 270 280 290 300
EDFVRKPDYA LSSVGASIDL QKTSHDYADR NTAYFWNRFS FWNYARPPTV
310 320 330 340 350
ILEPHVFPGN CWAFEGDQGQ VVIQLPGRVQ LSDITLQHPP PSVEHTGGAN
360 370 380 390 400
SAPRDFAVFG LQVYDETEVS LGKFTFDVEK SEIQTFHLQN DPPAAFPKVK
410 420 430
IQILSNWGHP RFTCLYRVRA HGVRTSEGAE GSAQGPH
Length:437
Mass (Da):48,165
Last modified:October 1, 2000 - v1
Checksum:i5D1E3BCAEEE8B702
GO

Sequence cautioni

The sequence AAC32052 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF262992 mRNA. Translation: AAF75267.1.
AF262993 Genomic DNA. Translation: AAF75268.1.
AL109827 Genomic DNA. No translation available.
AF043344 Genomic DNA. Translation: AAC32052.1. Sequence problems.
CCDSiCCDS13259.1.
RefSeqiNP_003107.1. NM_003116.2.
UniGeneiHs.123159.

Genome annotation databases

EnsembliENST00000374273; ENSP00000363391; ENSG00000061656.
GeneIDi6676.
KEGGihsa:6676.
UCSCiuc002xdb.2. human.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiSPAG4_HUMAN
AccessioniPrimary (citable) accession number: Q9NPE6
Secondary accession number(s): O43648
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 17, 2003
Last sequence update: October 1, 2000
Last modified: July 5, 2017
This is version 126 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

Although transmembrane domains are strongly predicted, they may rather represent hydrophobic globular domains associated with microtubules.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot