Q9NPE2 (NGRN_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Neugrin

Alternative name(s):
Mesenchymal stem cell protein DSC92
Neurite outgrowth-associated protein
Spinal cord-derived protein FI58G

Gene names

Name:	NGRN
Synonyms:	FI58G
ORF Names:	HT020

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	291 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be involved in neuronal differentiation.
Subcellular location	Nucleus. Secreted Potential Ref.1.
Tissue specificity	Expressed at high levels in heart, brain and skeletal muscle. In brain, mainly expressed in neurons rather than glial cells. Ref.1
Induction	Highly up-regulated in neuroblastostoma cells by retinoic acid treatment inducing neurite outgrowth.
Sequence similarities	Belongs to the neugrin family.
Sequence caution	The sequence AAF65447.1 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS. The sequence AAG09725.1 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS. The sequence AAG09725.1 differs from that shown. Reason: Frameshift at position 47. The sequence AAH01682.1 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS. The sequence AAH07222.1 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS. The sequence AAH09389.1 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS. The sequence AAH17192.1 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS. The sequence BAB21533.1 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS. The sequence BAB21533.1 differs from that shown. Reason: Frameshift at position 47. The sequence BAG35525.1 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS. The sequence BAG35525.1 differs from that shown. Reason: N-terminus does not match isoform 2. The sequence CAB96088.1 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS. The sequence CAD39160.1 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS.

Ontologies

Keywords
Biological process	Differentiation Neurogenesis
Cellular component	Nucleus Secreted
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Signal
Molecular function	Developmental protein
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	neuron differentiation Non-traceable author statement Ref.1. Source: UniProtKB
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Non-traceable author statement Ref.1. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9NPE2-2)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9NPE2-3)

The sequence of this isoform differs from the canonical sequence as follows:
     55-64: STLKRQKQAI → RYRLLPGPSA
     65-291: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 15	15	Potential
	Chain	16 – 291	276	Neugrin		PRO_0000294483
Amino acid modifications
	Modified residue	41	1	Phosphoserine Ref.11
	Glycosylation	158	1	N-linked (GlcNAc...) Potential
	Glycosylation	186	1	N-linked (GlcNAc...) Potential
	Glycosylation	268	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	55 – 64	10	STLKRQKQAI → RYRLLPGPSA in isoform 2.		VSP_039710
	Alternative sequence	65 – 291	227	Missing in isoform 2.		VSP_039711
	Natural variant	174	1	L → F. Corresponds to variant rs11073922 [ dbSNP | Ensembl ].		VAR_053905
	Natural variant	267	1	D → G. Corresponds to variant rs16944113 [ dbSNP | Ensembl ].		VAR_053906
Experimental info
	Sequence conflict	28	1	A → C in BG719592. Ref.2
	Sequence conflict	32	1	P → R in AAG09725. Ref.7

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 5, 2010. Version 2. Checksum: F0480D6D94BE2ACE	FASTA	291	32,408
	10 20 30 40 50 60 MAVTLSLLLG GRVCAAVTRC GFATRGVAGP GPIGREPDPD SDWEPEEREL QEVESTLKRQ 70 80 90 100 110 120 KQAIRFQKIR RQMEAPGAPP RTLTWEAMEQ IRYLHEEFPE SWSVPRLAEG FDVSTDVIRR 130 140 150 160 170 180 VLKSKFLPTL EQKLKQDQKV LKKAGLAHSL QHLRGSGNTS KLLPAGHSVS GSLLMPGHEA 190 200 210 220 230 240 SSKDPNHSTA LKVIESDTHR TNTPRRRKGR NKEIQDLEES FVPVAAPLGH PRELQKYSSD 250 260 270 280 290 SESPRGTGSG ALPSGQKLEE LKAEEPDNFS SKVVQRGREF FDSNGNFLYR I
	Isoform 2 [UniParc]. Checksum: 1BA91466FF84D107	FASTA	64	6,851
	10 20 30 40 50 60 MAVTLSLLLG GRVCAAVTRC GFATRGVAGP GPIGREPDPD SDWEPEEREL QEVERYRLLP GPSA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Two novel genes, human neugrin and mouse m-neugrin, are upregulated with neuronal differentiation in neuroblastoma cells." Ishigaki S., Niwa J., Yoshihara T., Mitsuma N., Doyu M., Sobue G. Biochem. Biophys. Res. Commun. 279:526-533(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[2]	"cDNA DSC92 expressed by osteogenic human mesenchymal stem cells." van den Bos C., Mbalaviele G., Thiede M. Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: Mesenchymal stem cell.
[3]	The European IMAGE consortium Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[4]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Kidney.
[5]	"Analysis of the DNA sequence and duplication history of human chromosome 15." Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K., Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N., Abouelleil A. , Arachchi H.M., Baradarani L., Birditt B., Bloom S., Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K., DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J., Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E., Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B., Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R., O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B., Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S., Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C. Nature 440:671-675(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Brain, Chondrosarcoma, Lung, Muscle, Placenta and Testis.
[7]	"Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning." Hu R.-M., Han Z.-G., Song H.-D., Peng Y.-D., Huang Q.-H., Ren S.-X., Gu Y.-J., Huang C.-H., Li Y.-B., Jiang C.-L., Fu G., Zhang Q.-H., Gu B.-W., Dai M., Mao Y.-F., Gao G.-F., Rong R., Ye M. , Zhou J., Xu S.-H., Gu J., Shi J.-X., Jin W.-R., Zhang C.-K., Wu T.-M., Huang G.-Y., Chen Z., Chen M.-D., Chen J.-L. Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 20-291 (ISOFORM 2).
[8]	Hu X., Xu Y., Peng X., Yuan J., Qiang B. Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 73-291 (ISOFORM 1).
[9]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: PARTIAL NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Neuroblastoma.
[10]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[11]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-41, MASS SPECTROMETRY. Tissue: Cervix carcinoma.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB029315 mRNA. Translation: BAB21533.1. Sequence problems. AF242770 mRNA. Translation: AAF65447.1. Sequence problems. AL360142 mRNA. Translation: CAB96088.1. Sequence problems. AL834503 mRNA. Translation: CAD39160.1. Sequence problems. AC091167 Genomic DNA. No translation available. BC001682 mRNA. Translation: AAH01682.1. Sequence problems. BC007222 mRNA. Translation: AAH07222.1. Sequence problems. BC009389 mRNA. Translation: AAH09389.1. Sequence problems. BC017192 mRNA. Translation: AAH17192.1. Sequence problems. BC096824 mRNA. Translation: AAH96824.1. BG719592 mRNA. No translation available. AF225423 mRNA. Translation: AAG09725.1. Sequence problems. AY049780 mRNA. Translation: AAL15437.1. AK312641 mRNA. Translation: BAG35525.1. Sequence problems.
IPI	IPI00651737. IPI00792526.
PIR	JC7563.
RefSeq	NP_001028260.2. NM_001033088.1.
UniGene	Hs.741160.
3D structure databases
ProteinModelPortal	Q9NPE2.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9NPE2. 2 interactions.
STRING	9606.ENSP00000368389.
PTM databases
PhosphoSite	Q9NPE2.
Polymorphism databases
DMDM	306526226.
Proteomic databases
PaxDb	Q9NPE2.
PRIDE	Q9NPE2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000379095; ENSP00000368389; ENSG00000182768.
GeneID	51335.
KEGG	hsa:51335.
UCSC	uc002bpf.1. human.
Organism-specific databases
CTD	51335.
GeneCards	GC15P090808.
H-InvDB	HIX0012575. HIX0172837.
HGNC	HGNC:18077. NGRN.
HPA	HPA041367.
neXtProt	NX_Q9NPE2.
PharmGKB	PA142671265.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG76499.
HOGENOM	HOG000252993.
HOVERGEN	HBG108182.
InParanoid	Q9NPE2.
OMA	KDPNHST.
OrthoDB	EOG49CQ8V.
Gene expression databases
Bgee	Q9NPE2.
CleanEx	HS_NGRN.
Genevestigator	Q9NPE2.
Family and domain databases
InterPro	IPR010487. Neugrin-related. [Graphical view]
Pfam	PF06413. Neugrin. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	51335.
NextBio	54759.

Entry information

Entry name

NGRN_HUMAN

Accession

Primary (citable) accession number: Q9NPE2
Secondary accession number(s): B2R6M8, Q4V9L7, Q9HBL4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	October 5, 2010
Last modified:	April 3, 2013
This is version 107 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.