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Q9NPE2 (NGRN_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Neugrin
Alternative name(s):
Mesenchymal stem cell protein DSC92
Neurite outgrowth-associated protein
Spinal cord-derived protein FI58G
Gene names
Name:NGRN
Synonyms:FI58G
ORF Names:HT020
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length291 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May be involved in neuronal differentiation.

Subcellular location

Nucleus. Secreted Potential Ref.1.

Tissue specificity

Expressed at high levels in heart, brain and skeletal muscle. In brain, mainly expressed in neurons rather than glial cells. Ref.1

Induction

Highly up-regulated in neuroblastostoma cells by retinoic acid treatment inducing neurite outgrowth.

Sequence similarities

Belongs to the neugrin family.

Sequence caution

The sequence AAF65447.1 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS.

The sequence AAG09725.1 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS.

The sequence AAG09725.1 differs from that shown. Reason: Frameshift at position 47.

The sequence AAH01682.1 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS.

The sequence AAH07222.1 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS.

The sequence AAH09389.1 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS.

The sequence AAH17192.1 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS.

The sequence BAB21533.1 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS.

The sequence BAB21533.1 differs from that shown. Reason: Frameshift at position 47.

The sequence BAG35525.1 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS.

The sequence BAG35525.1 differs from that shown. Reason: N-terminus does not match isoform 2.

The sequence CAB96088.1 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS.

The sequence CAD39160.1 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS.

Ontologies

Keywords
   Biological processDifferentiation
Neurogenesis
   Cellular componentNucleus
Secreted
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainSignal
   Molecular functionDevelopmental protein
   PTMGlycoprotein
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processneuron differentiation

Non-traceable author statement Ref.1. Source: UniProtKB

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

nucleus

Non-traceable author statement Ref.1. Source: UniProtKB

   Molecular_functionpoly(A) RNA binding

Inferred from direct assay PubMed 22658674PubMed 22681889. Source: UniProtKB

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9NPE2-2)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9NPE2-3)

The sequence of this isoform differs from the canonical sequence as follows:
     55-64: STLKRQKQAI → RYRLLPGPSA
     65-291: Missing.
Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1515 Potential
Chain16 – 291276Neugrin
PRO_0000294483

Amino acid modifications

Modified residue411Phosphoserine Ref.11
Glycosylation1581N-linked (GlcNAc...) Potential
Glycosylation1861N-linked (GlcNAc...) Potential
Glycosylation2681N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence55 – 6410STLKRQKQAI → RYRLLPGPSA in isoform 2.
VSP_039710
Alternative sequence65 – 291227Missing in isoform 2.
VSP_039711
Natural variant1741L → F.
Corresponds to variant rs11073922 [ dbSNP | Ensembl ].
VAR_053905
Natural variant2671D → G.
Corresponds to variant rs16944113 [ dbSNP | Ensembl ].
VAR_053906

Experimental info

Sequence conflict281A → C in BG719592. Ref.2
Sequence conflict321P → R in AAG09725. Ref.7

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 5, 2010. Version 2.
Checksum: F0480D6D94BE2ACE

FASTA29132,408
        10         20         30         40         50         60 
MAVTLSLLLG GRVCAAVTRC GFATRGVAGP GPIGREPDPD SDWEPEEREL QEVESTLKRQ 

        70         80         90        100        110        120 
KQAIRFQKIR RQMEAPGAPP RTLTWEAMEQ IRYLHEEFPE SWSVPRLAEG FDVSTDVIRR 

       130        140        150        160        170        180 
VLKSKFLPTL EQKLKQDQKV LKKAGLAHSL QHLRGSGNTS KLLPAGHSVS GSLLMPGHEA 

       190        200        210        220        230        240 
SSKDPNHSTA LKVIESDTHR TNTPRRRKGR NKEIQDLEES FVPVAAPLGH PRELQKYSSD 

       250        260        270        280        290 
SESPRGTGSG ALPSGQKLEE LKAEEPDNFS SKVVQRGREF FDSNGNFLYR I 

« Hide

Isoform 2 [UniParc].

Checksum: 1BA91466FF84D107
Show »

FASTA646,851

References

« Hide 'large scale' references
[1]"Two novel genes, human neugrin and mouse m-neugrin, are upregulated with neuronal differentiation in neuroblastoma cells."
Ishigaki S., Niwa J., Yoshihara T., Mitsuma N., Doyu M., Sobue G.
Biochem. Biophys. Res. Commun. 279:526-533(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[2]"cDNA DSC92 expressed by osteogenic human mesenchymal stem cells."
van den Bos C., Mbalaviele G., Thiede M.
Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Tissue: Mesenchymal stem cell.
[3]The European IMAGE consortium
Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[4]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Kidney.
[5]"Analysis of the DNA sequence and duplication history of human chromosome 15."
Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K., Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N., Abouelleil A. expand/collapse author list , Arachchi H.M., Baradarani L., Birditt B., Bloom S., Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K., DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J., Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E., Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B., Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R., O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B., Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S., Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C.
Nature 440:671-675(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Brain, Chondrosarcoma, Lung, Muscle, Placenta and Testis.
[7]"Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning."
Hu R.-M., Han Z.-G., Song H.-D., Peng Y.-D., Huang Q.-H., Ren S.-X., Gu Y.-J., Huang C.-H., Li Y.-B., Jiang C.-L., Fu G., Zhang Q.-H., Gu B.-W., Dai M., Mao Y.-F., Gao G.-F., Rong R., Ye M. expand/collapse author list , Zhou J., Xu S.-H., Gu J., Shi J.-X., Jin W.-R., Zhang C.-K., Wu T.-M., Huang G.-Y., Chen Z., Chen M.-D., Chen J.-L.
Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 20-291 (ISOFORM 2).
[8]Hu X., Xu Y., Peng X., Yuan J., Qiang B.
Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 73-291 (ISOFORM 1).
[9]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: PARTIAL NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Neuroblastoma.
[10]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[11]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-41, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB029315 mRNA. Translation: BAB21533.1. Sequence problems.
AF242770 mRNA. Translation: AAF65447.1. Sequence problems.
AL360142 mRNA. Translation: CAB96088.1. Sequence problems.
AL834503 mRNA. Translation: CAD39160.1. Sequence problems.
AC091167 Genomic DNA. No translation available.
BC001682 mRNA. Translation: AAH01682.1. Sequence problems.
BC007222 mRNA. Translation: AAH07222.1. Sequence problems.
BC009389 mRNA. Translation: AAH09389.1. Sequence problems.
BC017192 mRNA. Translation: AAH17192.1. Sequence problems.
BC096824 mRNA. Translation: AAH96824.1.
BG719592 mRNA. No translation available.
AF225423 mRNA. Translation: AAG09725.1. Sequence problems.
AY049780 mRNA. Translation: AAL15437.1.
AK312641 mRNA. Translation: BAG35525.1. Sequence problems.
CCDSCCDS32329.1. [Q9NPE2-2]
PIRJC7563.
RefSeqNP_001028260.2. NM_001033088.1. [Q9NPE2-2]
UniGeneHs.135471.
Hs.715556.

3D structure databases

ProteinModelPortalQ9NPE2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid119482. 2 interactions.
IntActQ9NPE2. 3 interactions.
MINTMINT-4714594.
STRING9606.ENSP00000368389.

PTM databases

PhosphoSiteQ9NPE2.

Polymorphism databases

DMDM306526226.

Proteomic databases

MaxQBQ9NPE2.
PaxDbQ9NPE2.
PRIDEQ9NPE2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000379095; ENSP00000368389; ENSG00000182768. [Q9NPE2-2]
GeneID51335.
KEGGhsa:51335.
UCSCuc002bpf.1. human. [Q9NPE2-2]

Organism-specific databases

CTD51335.
GeneCardsGC15P090808.
H-InvDBHIX0012575.
HIX0172837.
HGNCHGNC:18077. NGRN.
HPAHPA041367.
neXtProtNX_Q9NPE2.
PharmGKBPA142671265.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG76499.
HOGENOMHOG000252993.
HOVERGENHBG108182.
InParanoidQ9NPE2.
OMATNTPRRQ.
OrthoDBEOG7QC7W1.
PhylomeDBQ9NPE2.
TreeFamTF324463.

Gene expression databases

BgeeQ9NPE2.
CleanExHS_NGRN.
GenevestigatorQ9NPE2.

Family and domain databases

InterProIPR010487. Neugrin-related.
[Graphical view]
PfamPF06413. Neugrin. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi51335.
NextBio54759.
PROQ9NPE2.

Entry information

Entry nameNGRN_HUMAN
AccessionPrimary (citable) accession number: Q9NPE2
Secondary accession number(s): B2R6M8, Q4V9L7, Q9HBL4
Entry history
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: October 5, 2010
Last modified: July 9, 2014
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 15

Human chromosome 15: entries, gene names and cross-references to MIM