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Q9NPD5 (SO1B3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Solute carrier organic anion transporter family member 1B3
Alternative name(s):
Liver-specific organic anion transporter 2
Short name=LST-2
Organic anion transporter 8
Organic anion-transporting polypeptide 8
Short name=OATP-8
Solute carrier family 21 member 8
Gene names
Name:SLCO1B3
Synonyms:LST2, OATP1B3, OATP8,SLC21A8
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length702 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Mediates the Na+-independent uptake of organic anions such as 17-beta-glucuronosyl estradiol, taurocholate, triiodothyronine (T3), leukotriene C4, dehydroepiandrosterone sulfate (DHEAS), methotrexate and sulfobromophthalein (BSP). Involved in the clearance of bile acids and organic anions from the liver. Ref.3

Subcellular location

Basolateral cell membrane; Multi-pass membrane protein.

Tissue specificity

Highly expressed in liver, in particular at the basolateral membrane of hepatocytes near the central vein. Not detected in other tissues. Highly expressed in some cancer cell lines derived from colon, pancreas, liver and gall bladder.

Post-translational modification

N-glycosylated. Ref.1

Involvement in disease

Hyperbilirubinemia, Rotor type (HBLRR) [MIM:237450]: An autosomal recessive form of primary conjugated hyperbilirubinemia. Affected individuals develop mild jaundice not associated with hemolysis shortly after birth or in childhood. They have delayed plasma clearance of the unconjugated anionic dye bromsulphthalein and prominent urinary excretion of coproporphyrin I. Hepatic pigmentation is normal.
Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.3

Sequence similarities

Belongs to the organo anion transporter (TC 2.A.60) family. [View classification]

Contains 1 Kazal-like domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 702702Solute carrier organic anion transporter family member 1B3
PRO_0000191053

Regions

Topological domain1 – 2828Cytoplasmic Potential
Transmembrane29 – 4820Helical; Name=1; Potential
Topological domain49 – 6719Extracellular Potential
Transmembrane68 – 8821Helical; Name=2; Potential
Topological domain89 – 946Cytoplasmic Potential
Transmembrane95 – 11925Helical; Name=3; Potential
Topological domain120 – 16849Extracellular Potential
Transmembrane169 – 19729Helical; Name=4; Potential
Topological domain198 – 21619Cytoplasmic Potential
Transmembrane217 – 23721Helical; Name=5; Potential
Topological domain238 – 25518Extracellular Potential
Transmembrane256 – 28025Helical; Name=6; Potential
Topological domain281 – 33151Cytoplasmic Potential
Transmembrane332 – 35322Helical; Name=7; Potential
Topological domain354 – 37320Extracellular Potential
Transmembrane374 – 39724Helical; Name=8; Potential
Topological domain398 – 4014Cytoplasmic Potential
Transmembrane402 – 42524Helical; Name=9; Potential
Topological domain426 – 537112Extracellular Potential
Transmembrane538 – 56023Helical; Name=10; Potential
Topological domain561 – 5699Cytoplasmic Potential
Transmembrane570 – 59526Helical; Name=11; Potential
Topological domain596 – 62934Extracellular Potential
Transmembrane630 – 64718Helical; Name=12; Potential
Topological domain648 – 69548Cytoplasmic Potential
Domain453 – 50856Kazal-like

Amino acid modifications

Glycosylation1341N-linked (GlcNAc...) Potential
Glycosylation1451N-linked (GlcNAc...) Potential
Glycosylation1511N-linked (GlcNAc...) Potential
Glycosylation4451N-linked (GlcNAc...) Potential
Glycosylation5031N-linked (GlcNAc...) Potential
Glycosylation5161N-linked (GlcNAc...) Potential
Disulfide bond459 ↔ 485 By similarity
Disulfide bond463 ↔ 474 By similarity
Disulfide bond465 ↔ 489 By similarity

Natural variations

Natural variant1121S → A.
Corresponds to variant rs4149117 [ dbSNP | Ensembl ].
VAR_024645
Natural variant2331M → I.
Corresponds to variant rs7311358 [ dbSNP | Ensembl ].
VAR_053672
Natural variant2561G → A.
Corresponds to variant rs60140950 [ dbSNP | Ensembl ].
VAR_062150
Natural variant2921I → M in a colorectal cancer sample; somatic mutation. Ref.4
VAR_036410
Natural variant5601V → A.
Corresponds to variant rs12299012 [ dbSNP | Ensembl ].
VAR_053673
Natural variant6471M → L in a colorectal cancer sample; somatic mutation. Ref.4
VAR_036411

Sequences

Sequence LengthMass (Da)Tools
Q9NPD5 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 6D2AE371D782C7FB

FASTA70277,403
        10         20         30         40         50         60 
MDQHQHLNKT AESASSEKKK TRRCNGFKMF LAALSFSYIA KALGGIIMKI SITQIERRFD 

        70         80         90        100        110        120 
ISSSLAGLID GSFEIGNLLV IVFVSYFGSK LHRPKLIGIG CLLMGTGSIL TSLPHFFMGY 

       130        140        150        160        170        180 
YRYSKETHIN PSENSTSSLS TCLINQTLSF NGTSPEIVEK DCVKESGSHM WIYVFMGNML 

       190        200        210        220        230        240 
RGIGETPIVP LGISYIDDFA KEGHSSLYLG SLNAIGMIGP VIGFALGSLF AKMYVDIGYV 

       250        260        270        280        290        300 
DLSTIRITPK DSRWVGAWWL GFLVSGLFSI ISSIPFFFLP KNPNKPQKER KISLSLHVLK 

       310        320        330        340        350        360 
TNDDRNQTAN LTNQGKNVTK NVTGFFQSLK SILTNPLYVI FLLLTLLQVS SFIGSFTYVF 

       370        380        390        400        410        420 
KYMEQQYGQS ASHANFLLGI ITIPTVATGM FLGGFIIKKF KLSLVGIAKF SFLTSMISFL 

       430        440        450        460        470        480 
FQLLYFPLIC ESKSVAGLTL TYDGNNSVAS HVDVPLSYCN SECNCDESQW EPVCGNNGIT 

       490        500        510        520        530        540 
YLSPCLAGCK SSSGIKKHTV FYNCSCVEVT GLQNRNYSAH LGECPRDNTC TRKFFIYVAI 

       550        560        570        580        590        600 
QVINSLFSAT GGTTFILLTV KIVQPELKAL AMGFQSMVIR TLGGILAPIY FGALIDKTCM 

       610        620        630        640        650        660 
KWSTNSCGAQ GACRIYNSVF FGRVYLGLSI ALRFPALVLY IVFIFAMKKK FQGKDTKASD 

       670        680        690        700 
NERKVMDEAN LEFLNNGEHF VPSAGTDSKT CNLDMQDNAA AN 

« Hide

References

« Hide 'large scale' references
[1]"Localization and genomic organization of a new hepatocellular organic anion transporting polypeptide."
Koenig J., Cui Y., Nies A.T., Keppler D.
J. Biol. Chem. 275:23161-23168(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], GLYCOSYLATION.
Tissue: Liver.
[2]"LST-2, a human liver-specific organic anion transporter, determines methotrexate sensitivity in gastrointestinal cancers."
Abe T., Unno M., Onogawa T., Tokui T., Kondo T.N., Nakagomi R., Adachi H., Fujiwara K., Okabe M., Suzuki T., Nunoki K., Sato E., Kakyo M., Nishio T., Sugita J., Asano N., Tanemoto M., Seki M. expand/collapse author list , Date F., Ono K., Kondo Y., Shiiba K., Suzuki M., Ohtani H., Shimosegawa T., Iinuma K., Nagura H., Ito S., Matsuno S.
Gastroenterology 120:1689-1699(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Liver.
[3]"Complete OATP1B1 and OATP1B3 deficiency causes human Rotor syndrome by interrupting conjugated bilirubin reuptake into the liver."
van de Steeg E., Stranecky V., Hartmannova H., Noskova L., Hrebicek M., Wagenaar E., van Esch A., de Waart D.R., Oude Elferink R.P., Kenworthy K.E., Sticova E., al-Edreesi M., Knisely A.S., Kmoch S., Jirsa M., Schinkel A.H.
J. Clin. Invest. 122:519-528(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INVOLVEMENT IN HBLRR.
[4]"The consensus coding sequences of human breast and colorectal cancers."
Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. expand/collapse author list , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E.
Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANTS [LARGE SCALE ANALYSIS] MET-292 AND LEU-647.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ400763 expand/collapse EMBL AC list , AJ400764, AJ400765, AJ400766, AJ400767, AJ400768, AJ400769, AJ400770, AJ400771, AJ400772, AJ400773, AJ400774, AJ400775, AJ400776 Genomic DNA. Translation: CAB97008.1.
AJ251506 mRNA. Translation: CAB96997.1.
AF187815 mRNA. Translation: AAG43445.1.
RefSeqNP_062818.1. NM_019844.3.
UniGeneHs.504966.

3D structure databases

ProteinModelPortalQ9NPD5.
SMRQ9NPD5. Positions 65-91, 205-232, 462-528, 558-598.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000261196.

Chemistry

BindingDBQ9NPD5.
ChEMBLCHEMBL1743121.
GuidetoPHARMACOLOGY1221.

Protein family/group databases

TCDB2.A.60.1.12. the organo anion transporter (oat) family.

PTM databases

PhosphoSiteQ9NPD5.

Polymorphism databases

DMDM27734563.

Proteomic databases

PaxDbQ9NPD5.
PRIDEQ9NPD5.

Protocols and materials databases

DNASU28234.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000261196; ENSP00000261196; ENSG00000111700.
ENST00000381545; ENSP00000370956; ENSG00000111700.
GeneID28234.
KEGGhsa:28234.
UCSCuc001rel.4. human.

Organism-specific databases

CTD28234.
GeneCardsGC12P020963.
HGNCHGNC:10961. SLCO1B3.
HPAHPA004943.
HPA050892.
MIM237450. phenotype.
605495. gene.
neXtProtNX_Q9NPD5.
Orphanet3111. Rotor syndrome.
PharmGKBPA35844.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG322696.
HOGENOMHOG000231269.
HOVERGENHBG063896.
InParanoidQ9NPD5.
KOK05043.
OMASHIGAIT.
OrthoDBEOG7GFB4C.
PhylomeDBQ9NPD5.
TreeFamTF317540.

Enzyme and pathway databases

ReactomeREACT_111217. Metabolism.
REACT_15518. Transmembrane transport of small molecules.

Gene expression databases

ArrayExpressQ9NPD5.
BgeeQ9NPD5.
CleanExHS_SLCO1B3.
GenevestigatorQ9NPD5.

Family and domain databases

InterProIPR002350. Kazal_dom.
IPR020846. MFS_dom.
IPR016196. MFS_dom_general_subst_transpt.
IPR004156. OA_transporter.
[Graphical view]
PANTHERPTHR11388. PTHR11388. 1 hit.
PfamPF07648. Kazal_2. 1 hit.
PF03137. OATP. 1 hit.
[Graphical view]
SMARTSM00280. KAZAL. 1 hit.
[Graphical view]
SUPFAMSSF103473. SSF103473. 5 hits.
TIGRFAMsTIGR00805. oat. 1 hit.
PROSITEPS51465. KAZAL_2. 1 hit.
PS50850. MFS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiSLCO1B3.
GenomeRNAi28234.
NextBio50556.
PROQ9NPD5.
SOURCESearch...

Entry information

Entry nameSO1B3_HUMAN
AccessionPrimary (citable) accession number: Q9NPD5
Entry history
Integrated into UniProtKB/Swiss-Prot: January 10, 2003
Last sequence update: October 1, 2000
Last modified: April 16, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 12

Human chromosome 12: entries, gene names and cross-references to MIM