Q9NPC3 (CIP1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
E3 ubiquitin-protein ligase CCNB1IP1
EC=6.3.2.-

Alternative name(s):
Cyclin-B1-interacting protein 1
Human enhancer of invasion 10

Gene names

Name:	CCNB1IP1
Synonyms:	C14orf18, HEI10

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	277 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	E3 ubiquitin-protein ligase. Modulates cyclin B levels and participates in the regulation of cell cycle progression through the G2 phase. Overexpression causes delayed entry into mitosis. Ref.1
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Binds CCNB1 and UBE2L3.
Subcellular location	Nucleus. Chromosome. Note: May associate with segregating chromosomes during metaphase and anaphase.
Tissue specificity	Highly expressed in heart. Detected at intermediate levels in liver and kidney, and at low levels in placenta, brain and lung. Ref.1
Post-translational modification	Ubiquitinated; autoubiquitinated. Ref.1 Phosphorylated by CDK1 on serine or threonine residues (in vitro). Ref.1
Sequence similarities	Contains 1 RING-type zinc finger.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Chromosome Nucleus
Domain	Coiled coil Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Ligase
PTM	Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	apoptotic process Inferred from electronic annotation. Source: Compara blastocyst formation Inferred from electronic annotation. Source: Compara chiasma assembly Inferred from electronic annotation. Source: Compara meiotic metaphase I Inferred from electronic annotation. Source: Compara organ growth Inferred from electronic annotation. Source: Compara protein ubiquitination Inferred from electronic annotation. Source: UniProtKB-UniPathway spermatid development Inferred from electronic annotation. Source: Compara
Cellular_component	chromosome Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ligase activity Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	P62972	2	EBI-745269,EBI-412964	From a different organism.
CCNB1	P14635	2	EBI-745269,EBI-495332

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 277	277	E3 ubiquitin-protein ligase CCNB1IP1		PRO_0000055874
Regions
	Zinc finger	4 – 51	48	RING-type; atypical
	Coiled coil	127 – 182	56	Potential
Experimental info
	Mutagenesis	28	1	C → A: Abrogates induction of filamentous growth in yeast; when associated with A-30 and A-33. Ref.1
	Mutagenesis	30	1	H → A: Abrogates induction of filamentous growth in yeast; when associated with A-28 and A-33. Ref.1
	Mutagenesis	33	1	C → A: Abrogates induction of filamentous growth in yeast; when associated with A-28 and A-30. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9NPC3 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 885723B01A35225A	FASTA	277	31,544
	10 20 30 40 50 60 MSLCEDMLLC NYRKCRIKLS GYAWVTACSH IFCDQHGSGE FSRSPAICPA CNSTLSGKLD 70 80 90 100 110 120 IVRTELSPSE EYKAMVLAGL RPEIVLDISS RALAFWTYQV HQERLYQEYN FSKAEGHLKQ 130 140 150 160 170 180 MEKIYTQQIQ SKDVELTSMK GEVTSMKKVL EEYKKKFSDI SEKLMERNRQ YQKLQGLYDS 190 200 210 220 230 240 LRLRNITIAN HEGTLEPSMI AQSGVLGFPL GNNSKFPLDN TPVRNRGDGD GDFQFRPFFA 250 260 270 GSPTAPEPSN SFFSFVSPSR ELEQQQVSSR AFKVKRI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel RING finger protein, human enhancer of invasion 10, alters mitotic progression through regulation of cyclin B levels." Toby G.G., Gherraby W., Coleman T.R., Golemis E.A. Mol. Cell. Biol. 23:2109-2122(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS OF CYS-28; HIS-30 AND CYS-33, FUNCTION, INTERACTION WITH UBE2L3 AND CCNB1, UBIQUITINATION, PHOSPHORYLATION, TISSUE SPECIFICITY. Tissue: Cervix carcinoma.
[2]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Small intestine.
[4]	"Full-length cDNA libraries and normalization." Li W.B., Gruber C., Jessee J., Polayes D. Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Fetal brain and Placenta.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung and Muscle.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF216381 mRNA. Translation: AAF36386.1. AL161994 mRNA. Translation: CAB82326.1. AK026233 mRNA. Translation: BAB15403.1. BX161423 mRNA. Translation: CAD61896.1. BX161424 mRNA. Translation: CAD61897.1. BX247978 mRNA. Translation: CAD62312.1. BC000369 mRNA. Translation: AAH00369.1. BC001218 mRNA. Translation: AAH01218.1. BC004435 mRNA. Translation: AAH04435.1.
IPI	IPI00009603.
PIR	T47153.
RefSeq	NP_067001.3. NM_021178.4. NP_878269.1. NM_182849.2. NP_878272.1. NM_182852.3.
UniGene	Hs.107003. Hs.741214.
3D structure databases
ProteinModelPortal	Q9NPC3.
SMR	Q9NPC3. Positions 22-59.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9NPC3. 4 interactions.
MINT	MINT-1450464.
STRING	9606.ENSP00000337396.
PTM databases
PhosphoSite	Q9NPC3.
Polymorphism databases
DMDM	37078756.
Proteomic databases
PaxDb	Q9NPC3.
PRIDE	Q9NPC3.
Protocols and materials databases
DNASU	57820.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000353689; ENSP00000337396; ENSG00000100814. ENST00000358932; ENSP00000351810; ENSG00000100814. ENST00000398160; ENSP00000381226; ENSG00000100814. ENST00000398163; ENSP00000381229; ENSG00000100814. ENST00000398169; ENSP00000381235; ENSG00000100814. ENST00000437553; ENSP00000409896; ENSG00000100814.
GeneID	57820.
KEGG	hsa:57820.
UCSC	uc001vwv.3. human.
Organism-specific databases
CTD	57820.
GeneCards	GC14M020779.
HGNC	HGNC:19437. CCNB1IP1.
MIM	608249. gene.
neXtProt	NX_Q9NPC3.
PharmGKB	PA134863884.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG27432.
HOGENOM	HOG000111677.
HOVERGEN	HBG050962.
InParanoid	Q9NPC3.
KO	K10639.
OMA	EPSMITQ.
OrthoDB	EOG41G357.
Enzyme and pathway databases
UniPathway	UPA00143.
Gene expression databases
ArrayExpress	Q9NPC3.
Bgee	Q9NPC3.
CleanEx	HS_CCNB1IP1.
Genevestigator	Q9NPC3.
GermOnline	ENSG00000100814. Homo sapiens.
Family and domain databases
PROSITE	PS00518. ZF_RING_1. False negative. PS50089. ZF_RING_2. False negative. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	57820.
NextBio	64776.
SOURCE	Search...

Entry information

Entry name

CIP1_HUMAN

Accession

Primary (citable) accession number: Q9NPC3

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2003
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 108 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.