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Q9NPC3 (CIP1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 118. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
E3 ubiquitin-protein ligase CCNB1IP1

EC=6.3.2.-
Alternative name(s):
Cyclin-B1-interacting protein 1
Human enhancer of invasion 10
Gene names
Name:CCNB1IP1
Synonyms:C14orf18, HEI10
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length277 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Ubiquitin E3 ligase that acts as a limiting factor for crossing-over during meiosis: required during zygonema to limit the colocalization of RNF212 with MutS-gamma-associated recombination sites and thereby establish early differentiation of crossover and non-crossover sites. Later, it is directed by MutL-gamma to stably accumulate at designated crossover sites. Probably promotes the dissociation of RNF212 and MutS-gamma to allow the progression of recombination and the implementation of the final steps of crossing over By similarity. Modulates cyclin-B levels and participates in the regulation of cell cycle progression through the G2 phase. Overexpression causes delayed entry into mitosis. Ref.1 Ref.7

E3 ubiquitin-protein ligase. Modulates cyclin B levels and participates in the regulation of cell cycle progression through the G2 phase. Overexpression causes delayed entry into mitosis. Ref.1 Ref.7

Pathway

Protein modification; protein ubiquitination.

Subunit structure

Interacts with CCNB1, UBE2L3 and NF2. Ref.1 Ref.6

Subcellular location

Nucleus. Chromosome. Note: Associates to the synaptonemal complex.

Tissue specificity

Highly expressed in heart. Detected at intermediate levels in liver and kidney, and at low levels in placenta, brain and lung. Ref.1

Post-translational modification

Ubiquitinated; autoubiquitinated. Ref.1

Phosphorylated by CDK1 on serine or threonine residues (in vitro). Ref.1

Sequence similarities

Contains 1 RING-type zinc finger.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

P629722EBI-745269,EBI-412964From a different organism.
CCNB1P146352EBI-745269,EBI-495332

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 277277E3 ubiquitin-protein ligase CCNB1IP1
PRO_0000055874

Regions

Zinc finger4 – 5148RING-type; atypical
Coiled coil127 – 18256 Potential

Experimental info

Mutagenesis281C → A: Abrogates induction of filamentous growth in yeast; when associated with A-30 and A-33. Ref.1
Mutagenesis301H → A: Abrogates induction of filamentous growth in yeast; when associated with A-28 and A-33. Ref.1
Mutagenesis331C → A: Abrogates induction of filamentous growth in yeast; when associated with A-28 and A-30. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9NPC3 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 885723B01A35225A

FASTA27731,544
        10         20         30         40         50         60 
MSLCEDMLLC NYRKCRIKLS GYAWVTACSH IFCDQHGSGE FSRSPAICPA CNSTLSGKLD 

        70         80         90        100        110        120 
IVRTELSPSE EYKAMVLAGL RPEIVLDISS RALAFWTYQV HQERLYQEYN FSKAEGHLKQ 

       130        140        150        160        170        180 
MEKIYTQQIQ SKDVELTSMK GEVTSMKKVL EEYKKKFSDI SEKLMERNRQ YQKLQGLYDS 

       190        200        210        220        230        240 
LRLRNITIAN HEGTLEPSMI AQSGVLGFPL GNNSKFPLDN TPVRNRGDGD GDFQFRPFFA 

       250        260        270 
GSPTAPEPSN SFFSFVSPSR ELEQQQVSSR AFKVKRI 

« Hide

References

« Hide 'large scale' references
[1]"A novel RING finger protein, human enhancer of invasion 10, alters mitotic progression through regulation of cyclin B levels."
Toby G.G., Gherraby W., Coleman T.R., Golemis E.A.
Mol. Cell. Biol. 23:2109-2122(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS OF CYS-28; HIS-30 AND CYS-33, FUNCTION, INTERACTION WITH UBE2L3 AND CCNB1, UBIQUITINATION, PHOSPHORYLATION, TISSUE SPECIFICITY.
Tissue: Cervix carcinoma.
[2]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Small intestine.
[4]"Full-length cDNA libraries and normalization."
Li W.B., Gruber C., Jessee J., Polayes D.
Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Fetal brain and Placenta.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Lung and Muscle.
[6]"A functional association between merlin and HEI10, a cell cycle regulator."
Gronholm M., Muranen T., Toby G.G., Utermark T., Hanemann C.O., Golemis E.A., Carpen O.
Oncogene 25:4389-4398(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH NF2.
[7]"HEI10 negatively regulates cell invasion by inhibiting cyclin B/Cdk1 and other promotility proteins."
Singh M.K., Nicolas E., Gherraby W., Dadke D., Lessin S., Golemis E.A.
Oncogene 26:4825-4832(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF216381 mRNA. Translation: AAF36386.1.
AL161994 mRNA. Translation: CAB82326.1.
AK026233 mRNA. Translation: BAB15403.1.
BX161423 mRNA. Translation: CAD61896.1.
BX161424 mRNA. Translation: CAD61897.1.
BX247978 mRNA. Translation: CAD62312.1.
BC000369 mRNA. Translation: AAH00369.1.
BC001218 mRNA. Translation: AAH01218.1.
BC004435 mRNA. Translation: AAH04435.1.
CCDSCCDS9547.1.
PIRT47153.
RefSeqNP_067001.3. NM_021178.4.
NP_878269.1. NM_182849.2.
NP_878272.1. NM_182852.3.
UniGeneHs.107003.
Hs.741214.

3D structure databases

ProteinModelPortalQ9NPC3.
SMRQ9NPC3. Positions 6-58.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid121779. 6 interactions.
IntActQ9NPC3. 4 interactions.
MINTMINT-1450464.
STRING9606.ENSP00000337396.

PTM databases

PhosphoSiteQ9NPC3.

Polymorphism databases

DMDM37078756.

Proteomic databases

PaxDbQ9NPC3.
PRIDEQ9NPC3.

Protocols and materials databases

DNASU57820.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000353689; ENSP00000337396; ENSG00000100814.
ENST00000358932; ENSP00000351810; ENSG00000100814.
ENST00000398160; ENSP00000381226; ENSG00000100814.
ENST00000398163; ENSP00000381229; ENSG00000100814.
ENST00000398169; ENSP00000381235; ENSG00000100814.
ENST00000437553; ENSP00000409896; ENSG00000100814.
GeneID57820.
KEGGhsa:57820.
UCSCuc001vwv.4. human.

Organism-specific databases

CTD57820.
GeneCardsGC14M020779.
HGNCHGNC:19437. CCNB1IP1.
MIM608249. gene.
neXtProtNX_Q9NPC3.
PharmGKBPA134863884.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG27432.
HOGENOMHOG000111677.
HOVERGENHBG050962.
InParanoidQ9NPC3.
KOK10639.
OMAIANHEGT.
OrthoDBEOG76MK99.
PhylomeDBQ9NPC3.
TreeFamTF328863.

Enzyme and pathway databases

UniPathwayUPA00143.

Gene expression databases

ArrayExpressQ9NPC3.
BgeeQ9NPC3.
CleanExHS_CCNB1IP1.
GenevestigatorQ9NPC3.

Family and domain databases

InterProIPR001841. Znf_RING.
[Graphical view]
PfamPF14634. zf-RING_5. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiCCNB1IP1.
GenomeRNAi57820.
NextBio64776.
PROQ9NPC3.
SOURCESearch...

Entry information

Entry nameCIP1_HUMAN
AccessionPrimary (citable) accession number: Q9NPC3
Entry history
Integrated into UniProtKB/Swiss-Prot: September 26, 2003
Last sequence update: October 1, 2000
Last modified: July 9, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 14

Human chromosome 14: entries, gene names and cross-references to MIM