Q9NPC1 (LT4R2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Leukotriene B4 receptor 2
Short name=LTB4-R 2
Short name=LTB4-R2

Alternative name(s):
LTB4 receptor JULF2
Leukotriene B4 receptor BLT2
Seven transmembrane receptor BLTR2

Gene names

Name:	LTB4R2
Synonyms:	BLT2R, BLTR2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	389 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Low-affinity receptor for leukotrienes including leukotriene B4. Mediates chemotaxis of granulocytes and macrophages. The response is mediated via G-proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of affinities for the leukotrienes is LTB4 > 12-epi-LTB4 > LTB5 > LTB3.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Widely expressed.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.
Caution	It is uncertain whether Met-1 or Met-32 is the initiator.
Sequence caution	The sequence BAB00610.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	chemotaxis Traceable author statement Ref.9. Source: ProtInc negative regulation of adenylate cyclase activity Traceable author statement Ref.9. Source: ProtInc
Cellular_component	integral to plasma membrane Traceable author statement Ref.1. Source: ProtInc
Molecular_function	leukotriene receptor activity Traceable author statement Ref.9. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9NPC1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9NPC1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-31: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 389	389	Leukotriene B4 receptor 2		PRO_0000069711
Regions
	Topological domain	1 – 55	55	Extracellular Potential
	Transmembrane	56 – 76	21	Helical; Name=1; Potential
	Topological domain	77 – 91	15	Cytoplasmic Potential
	Transmembrane	92 – 112	21	Helical; Name=2; Potential
	Topological domain	113 – 127	15	Extracellular Potential
	Transmembrane	128 – 148	21	Helical; Name=3; Potential
	Topological domain	149 – 172	24	Cytoplasmic Potential
	Transmembrane	173 – 193	21	Helical; Name=4; Potential
	Topological domain	194 – 216	23	Extracellular Potential
	Transmembrane	217 – 237	21	Helical; Name=5; Potential
	Topological domain	238 – 256	19	Cytoplasmic Potential
	Transmembrane	257 – 277	21	Helical; Name=6; Potential
	Topological domain	278 – 306	29	Extracellular Potential
	Transmembrane	307 – 327	21	Helical; Name=7; Potential
	Topological domain	328 – 389	62	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	41	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	1 – 31	31	Missing in isoform 2.		VSP_044885

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 8D6BC5A73A42F544	FASTA	389	41,525
	10 20 30 40 50 60 MAPSHRASQV GFCPTPERPL WRLPPTCRPR RMSVCYRPPG NETLLSWKTS RATGTAFLLL 70 80 90 100 110 120 AALLGLPGNG FVVWSLAGWR PARGRPLAAT LVLHLALADG AVLLLTPLFV AFLTRQAWPL 130 140 150 160 170 180 GQAGCKAVYY VCALSMYASV LLTGLLSLQR CLAVTRPFLA PRLRSPALAR RLLLAVWLAA 190 200 210 220 230 240 LLLAVPAAVY RHLWRDRVCQ LCHPSPVHAA AHLSLETLTA FVLPFGLMLG CYSVTLARLR 250 260 270 280 290 300 GARWGSGRHG ARVGRLVSAI VLAFGLLWAP YHAVNLLQAV AALAPPEGAL AKLGGAGQAA 310 320 330 340 350 360 RAGTTALAFF SSSVNPVLYV FTAGDLLPRA GPRFLTRLFE GSGEARGGGR SREGTMELRT 370 380 TPQLKVVGQG RGNGDPGGGM EKDGPEWDL
	Isoform 2 [UniParc]. Checksum: 5F5D98179CE00F3D	FASTA	358	37,942
	10 20 30 40 50 60 MSVCYRPPGN ETLLSWKTSR ATGTAFLLLA ALLGLPGNGF VVWSLAGWRP ARGRPLAATL 70 80 90 100 110 120 VLHLALADGA VLLLTPLFVA FLTRQAWPLG QAGCKAVYYV CALSMYASVL LTGLLSLQRC 130 140 150 160 170 180 LAVTRPFLAP RLRSPALARR LLLAVWLAAL LLAVPAAVYR HLWRDRVCQL CHPSPVHAAA 190 200 210 220 230 240 HLSLETLTAF VLPFGLMLGC YSVTLARLRG ARWGSGRHGA RVGRLVSAIV LAFGLLWAPY 250 260 270 280 290 300 HAVNLLQAVA ALAPPEGALA KLGGAGQAAR AGTTALAFFS SSVNPVLYVF TAGDLLPRAG 310 320 330 340 350 PRFLTRLFEG SGEARGGGRS REGTMELRTT PQLKVVGQGR GNGDPGGGME KDGPEWDL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of cDNA encoding a novel human leukotriene B4 receptor." Tryselius Y., Nilsson N.E., Kotarsky K., Olde B., Owman C. Biochem. Biophys. Res. Commun. 274:377-382(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"A second leukotriene B4 receptor, BLT2: a new therapeutic target in inflammation and immunological disorders." Yokomizo T., Kato K., Terawaki K., Izumi T., Shimizu T. J. Exp. Med. 192:421-432(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]	"Probable G-protein coupled receptor." Hirose M., Urakawa I., Yamano S., Okazaki H. Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[4]	"Identification and characterisation of a novel LTB4 receptor." Volpe F., Patel K., Barnes A., Sanseau P., Cousens D., Green A., Marshall F. Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Kopatz S.A., Aronstam R.S., Sharma S.V. Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[6]	"The DNA sequence and analysis of human chromosome 14." Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H. , Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J. Nature 421:601-607(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	"A novel hepatointestinal leukotriene B4 receptor: cloning and functional characterization." Wang S., Gustafson E., Pang L., Qiao X., Behan J., Maguire M., Bayne M., Laz T. J. Biol. Chem. 275:40686-40694(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 32-389 (ISOFORM 1).
[9]	"Molecular cloning and characterization of another leukotriene B4 receptor." Kamohara M., Takasaki J., Matsumoto M., Saito T., Ohishi T., Ishii H., Furuichi K. J. Biol. Chem. 275:27000-27004(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 32-389 (ISOFORM 1).
[10]	"Identification of G protein-coupled receptor genes from the human genome sequence." Takeda S., Kadowaki S., Haga T., Takaesu H., Mitaku S. FEBS Lett. 520:97-101(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 32-389.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF277230 mRNA. Translation: AAF81261.1. AB029892 mRNA. Translation: BAA99553.1. AB008193 Genomic DNA. Translation: BAB00610.1. Different initiation. AB032558 mRNA. Translation: BAD83590.1. AJ278605 Genomic DNA. Translation: CAB96134.1. AY268433 Genomic DNA. Translation: AAP23200.1. AL096870 Genomic DNA. No translation available. CH471078 Genomic DNA. Translation: EAW66030.1. AF308571 mRNA. Translation: AAG40857.1. AB044402 mRNA. Translation: BAA99539.1. AB083604 Genomic DNA. Translation: BAB89317.1.
IPI	IPI00022867. IPI00980914.
PIR	JC7356.
RefSeq	NP_001158164.1. NM_001164692.2. NP_062813.2. NM_019839.4.
UniGene	Hs.642693. Hs.657595.
3D structure databases
ProteinModelPortal	Q9NPC1.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000337731.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	Q9NPC1.
Polymorphism databases
DMDM	23821812.
Proteomic databases
PaxDb	Q9NPC1.
PRIDE	Q9NPC1.
Protocols and materials databases
DNASU	56413.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000528054; ENSP00000432146; ENSG00000213906. ENST00000533293; ENSP00000433290; ENSG00000213906. ENST00000543919; ENSP00000445772; ENSG00000213906.
GeneID	56413.
KEGG	hsa:56413.
UCSC	uc001wor.3. human.
Organism-specific databases
CTD	56413.
GeneCards	GC14P024778.
H-InvDB	HIX0131234.
HGNC	HGNC:19260. LTB4R2.
HPA	CAB022689. HPA029680.
MIM	605773. gene.
neXtProt	NX_Q9NPC1.
PharmGKB	PA134910672.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG308968.
HOGENOM	HOG000008594.
HOVERGEN	HBG104134.
InParanoid	Q9NPC1.
KO	K04297.
OMA	PRAGPRF.
OrthoDB	EOG48D0W1.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction.
Gene expression databases
ArrayExpress	Q9NPC1.
Bgee	Q9NPC1.
CleanEx	HS_LTB4R2.
Genevestigator	Q9NPC1.
GermOnline	ENSG00000196943. Homo sapiens.
Family and domain databases
InterPro	IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. IPR003981. Leukotriene_B4_rcpt. IPR003982. Leukotriene_B4_typ-2_rcpt. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR01478. LTB2RECEPTOR. PR01476. LTBRECEPTOR.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. False negative. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	Q9NPC1.
ChEMBL	CHEMBL3191.
GenomeRNAi	56413.
NextBio	61997.
SOURCE	Search...

Entry information

Entry name

LT4R2_HUMAN

Accession

Primary (citable) accession number: Q9NPC1
Secondary accession number(s): Q5KU28, Q9NPE5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 10, 2002
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 110 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.