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Protein

Partitioning defective 6 homolog alpha

Gene

PARD6A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Adapter protein involved in asymmetrical cell division and cell polarization processes. Probably involved in the formation of epithelial tight junctions. Association with PARD3 may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins (PubMed:10873802). Regulates centrosome organization and function. Essential for the centrosomal recruitment of key proteins that control centrosomal microtubule organization (PubMed:20719959).2 Publications

GO - Molecular functioni

  • GTP-dependent protein binding Source: UniProtKB
  • Rho GTPase binding Source: UniProtKB
  • transcription factor binding Source: ProtInc

GO - Biological processi

  • bicellular tight junction assembly Source: Reactome
  • cell-cell junction maintenance Source: UniProtKB
  • cell cycle Source: UniProtKB-KW
  • cell division Source: UniProtKB-KW
  • centrosome organization Source: UniProtKB
  • negative regulation of protein phosphorylation Source: Ensembl
  • positive regulation of protein localization to centrosome Source: UniProtKB
  • transforming growth factor beta receptor signaling pathway Source: Reactome
  • viral process Source: ProtInc
  • Wnt signaling pathway, planar cell polarity pathway Source: Reactome
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division

Enzyme and pathway databases

BioCyciZFISH:ENSG00000102981-MONOMER.
ReactomeiR-HSA-2173791. TGF-beta receptor signaling in EMT (epithelial to mesenchymal transition).
R-HSA-420029. Tight junction interactions.
R-HSA-4608870. Asymmetric localization of PCP proteins.
SignaLinkiQ9NPB6.
SIGNORiQ9NPB6.

Names & Taxonomyi

Protein namesi
Recommended name:
Partitioning defective 6 homolog alpha
Short name:
PAR-6
Short name:
PAR-6 alpha
Short name:
PAR-6A
Alternative name(s):
PAR6C
Tax interaction protein 40
Short name:
TIP-40
Gene namesi
Name:PARD6A
Synonyms:PAR6A
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:15943. PARD6A.

Subcellular locationi

GO - Cellular componenti

  • actin cytoskeleton Source: HPA
  • apical part of cell Source: Ensembl
  • bicellular tight junction Source: UniProtKB
  • cell cortex Source: Ensembl
  • cell junction Source: HPA
  • centriolar satellite Source: UniProtKB
  • centrosome Source: UniProtKB
  • cytoplasm Source: HPA
  • cytosol Source: Reactome
  • nucleus Source: UniProtKB
  • plasma membrane Source: Reactome
  • protein complex Source: Ensembl
  • ruffle Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane, Tight junction

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi19K → A: Loss of interaction with ECT2 and PRKCI. 2 Publications1
Mutagenesisi28R → A: Slight decrease of interaction with PRKCI. Loss of interaction with PRKCI; when associated with A-89. 1 Publication1
Mutagenesisi89R → A: Slight decrease of interaction with PRKCI. Loss of interaction with PRKCI; when associated with A-28. 1 Publication1

Organism-specific databases

DisGeNETi50855.
OpenTargetsiENSG00000102981.
PharmGKBiPA32937.

Polymorphism and mutation databases

BioMutaiPARD6A.
DMDMi30913215.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001125131 – 346Partitioning defective 6 homolog alphaAdd BLAST346

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei278PhosphoserineCombined sources1
Modified residuei345PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated by the TGF-beta receptor.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9NPB6.
PaxDbiQ9NPB6.
PeptideAtlasiQ9NPB6.
PRIDEiQ9NPB6.

PTM databases

iPTMnetiQ9NPB6.
PhosphoSitePlusiQ9NPB6.

Expressioni

Tissue specificityi

Expressed in pancreas, skeletal muscle, brain and heart. Weakly expressed in kidney and placenta.

Gene expression databases

BgeeiENSG00000102981.
CleanExiHS_PARD6A.
ExpressionAtlasiQ9NPB6. baseline and differential.
GenevisibleiQ9NPB6. HS.

Organism-specific databases

HPAiCAB009733.
HPA041551.

Interactioni

Subunit structurei

Interacts with MAP2K5 (By similarity). Interacts with PARD3. Interacts with GTP-bound forms of CDC42, ARHQ/TC10 and RAC1. Interacts with the N-terminal part of PRKCI and PRKCZ. Part of a complex with PARD3, CDC42 or RAC1 and PRKCI or PRKCZ. Part of a complex with LLGL1 and PRKCI (By similarity). Interacts with human T-cell leukemia virus type I TAX protein. Interacts with MPP5 and CRB3. Interacts with TGFBR1; involved in TGF-beta induced epithelial to mesenchymal transition. Interacts with ECT2 ('Thr-359' phosphorylated form) and PRKCI. Interacts with DCTN1 and PCM1 (PubMed:20719959).By similarity13 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
B2RCZ45EBI-81876,EBI-10175803
CDC42P609537EBI-81876,EBI-81752
CDH5P331514EBI-81876,EBI-2903122
GRB2P629933EBI-10693102,EBI-401755
PARD3Q8TEW09EBI-81876,EBI-81968
PIK3R3Q925693EBI-10693102,EBI-79893
PNMA1Q8ND902EBI-81876,EBI-302345
PRKCIP4174312EBI-81876,EBI-286199
PRKCZQ055138EBI-81876,EBI-295351
RAC1P630002EBI-81876,EBI-413628
YWHAHQ049172EBI-81876,EBI-306940

GO - Molecular functioni

  • GTP-dependent protein binding Source: UniProtKB
  • Rho GTPase binding Source: UniProtKB
  • transcription factor binding Source: ProtInc

Protein-protein interaction databases

BioGridi119157. 56 interactors.
DIPiDIP-31312N.
IntActiQ9NPB6. 47 interactors.
MINTiMINT-191836.
STRINGi9606.ENSP00000219255.

Structurei

Secondary structure

1346
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi15 – 22Combined sources8
Beta strandi25 – 32Combined sources8
Helixi33 – 35Combined sources3
Helixi39 – 49Combined sources11
Beta strandi58 – 62Combined sources5
Beta strandi68 – 70Combined sources3
Helixi74 – 80Combined sources7
Beta strandi83 – 86Combined sources4
Beta strandi88 – 93Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WMHX-ray1.50B14-95[»]
ProteinModelPortaliQ9NPB6.
SMRiQ9NPB6.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9NPB6.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini15 – 95PB1PROSITE-ProRule annotationAdd BLAST81
Domaini133 – 150Pseudo-CRIBAdd BLAST18
Domaini157 – 250PDZPROSITE-ProRule annotationAdd BLAST94

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 116Interaction with PRKCI and PRKCZAdd BLAST116
Regioni126 – 253Interaction with PARD3 and CDC42By similarityAdd BLAST128

Domaini

The pseudo-CRIB domain together with the PDZ domain is required for the interaction with Rho small GTPases.By similarity
The PB1 domain mediates interactions with MAP2K5.By similarity
The PDZ domain mediates the interaction with CRB3.1 Publication

Sequence similaritiesi

Belongs to the PAR6 family.Curated
Contains 1 PB1 domain.PROSITE-ProRule annotation
Contains 1 PDZ (DHR) domain.PROSITE-ProRule annotation
Contains 1 pseudo-CRIB domain.Curated

Phylogenomic databases

eggNOGiKOG3606. Eukaryota.
ENOG410XRJ3. LUCA.
GeneTreeiENSGT00390000005255.
HOGENOMiHOG000231716.
HOVERGENiHBG053509.
InParanoidiQ9NPB6.
KOiK06093.
OMAiCWDLHPG.
OrthoDBiEOG091G0FFH.
PhylomeDBiQ9NPB6.
TreeFamiTF312899.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
InterProiIPR000270. PB1_dom.
IPR001478. PDZ.
[Graphical view]
PfamiPF00564. PB1. 1 hit.
PF00595. PDZ. 1 hit.
[Graphical view]
SMARTiSM00666. PB1. 1 hit.
SM00228. PDZ. 1 hit.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 1 hit.
PROSITEiPS51745. PB1. 1 hit.
PS50106. PDZ. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9NPB6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MARPQRTPAR SPDSIVEVKS KFDAEFRRFA LPRASVSGFQ EFSRLLRAVH
60 70 80 90 100
QIPGLDVLLG YTDAHGDLLP LTNDDSLHRA LASGPPPLRL LVQKRAEADS
110 120 130 140 150
SGLAFASNSL QRRKKGLLLR PVAPLRTRPP LLISLPQDFR QVSSVIDVDL
160 170 180 190 200
LPETHRRVRL HKHGSDRPLG FYIRDGMSVR VAPQGLERVP GIFISRLVRG
210 220 230 240 250
GLAESTGLLA VSDEILEVNG IEVAGKTLDQ VTDMMVANSH NLIVTVKPAN
260 270 280 290 300
QRNNVVRGAS GRLTGPPSAG PGPAEPDSDD DSSDLVIENR QPPSSNGLSQ
310 320 330 340
GPPCWDLHPG CRHPGTRSSL PSLDDQEQAS SGWGSRIRGD GSGFSL
Length:346
Mass (Da):37,388
Last modified:October 1, 2000 - v1
Checksum:i9241E0EDC3694AD4
GO
Isoform 2 (identifier: Q9NPB6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     96-96: Missing.

Show »
Length:345
Mass (Da):37,317
Checksum:i035F9FA6BB62F085
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_050454286V → I.Corresponds to variant rs35356834dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00745996Missing in isoform 2. 4 Publications1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ277095 mRNA. Translation: CAB85490.1.
AF265565 mRNA. Translation: AAF75548.1.
AF252292 mRNA. Translation: AAF71529.1.
AB043634 mRNA. Translation: BAA96235.1.
AB041642 mRNA. Translation: BAB16105.1.
BC015626 mRNA. Translation: AAH15626.1.
AF028827 mRNA. Translation: AAB84252.1.
CCDSiCCDS10843.1. [Q9NPB6-1]
CCDS45514.1. [Q9NPB6-2]
RefSeqiNP_001032358.1. NM_001037281.1. [Q9NPB6-2]
NP_058644.1. NM_016948.2. [Q9NPB6-1]
UniGeneiHs.112933.

Genome annotation databases

EnsembliENST00000219255; ENSP00000219255; ENSG00000102981. [Q9NPB6-1]
ENST00000458121; ENSP00000392388; ENSG00000102981. [Q9NPB6-2]
GeneIDi50855.
KEGGihsa:50855.
UCSCiuc002ets.3. human. [Q9NPB6-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ277095 mRNA. Translation: CAB85490.1.
AF265565 mRNA. Translation: AAF75548.1.
AF252292 mRNA. Translation: AAF71529.1.
AB043634 mRNA. Translation: BAA96235.1.
AB041642 mRNA. Translation: BAB16105.1.
BC015626 mRNA. Translation: AAH15626.1.
AF028827 mRNA. Translation: AAB84252.1.
CCDSiCCDS10843.1. [Q9NPB6-1]
CCDS45514.1. [Q9NPB6-2]
RefSeqiNP_001032358.1. NM_001037281.1. [Q9NPB6-2]
NP_058644.1. NM_016948.2. [Q9NPB6-1]
UniGeneiHs.112933.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WMHX-ray1.50B14-95[»]
ProteinModelPortaliQ9NPB6.
SMRiQ9NPB6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119157. 56 interactors.
DIPiDIP-31312N.
IntActiQ9NPB6. 47 interactors.
MINTiMINT-191836.
STRINGi9606.ENSP00000219255.

PTM databases

iPTMnetiQ9NPB6.
PhosphoSitePlusiQ9NPB6.

Polymorphism and mutation databases

BioMutaiPARD6A.
DMDMi30913215.

Proteomic databases

MaxQBiQ9NPB6.
PaxDbiQ9NPB6.
PeptideAtlasiQ9NPB6.
PRIDEiQ9NPB6.

Protocols and materials databases

DNASUi50855.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000219255; ENSP00000219255; ENSG00000102981. [Q9NPB6-1]
ENST00000458121; ENSP00000392388; ENSG00000102981. [Q9NPB6-2]
GeneIDi50855.
KEGGihsa:50855.
UCSCiuc002ets.3. human. [Q9NPB6-1]

Organism-specific databases

CTDi50855.
DisGeNETi50855.
GeneCardsiPARD6A.
HGNCiHGNC:15943. PARD6A.
HPAiCAB009733.
HPA041551.
MIMi607484. gene.
neXtProtiNX_Q9NPB6.
OpenTargetsiENSG00000102981.
PharmGKBiPA32937.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3606. Eukaryota.
ENOG410XRJ3. LUCA.
GeneTreeiENSGT00390000005255.
HOGENOMiHOG000231716.
HOVERGENiHBG053509.
InParanoidiQ9NPB6.
KOiK06093.
OMAiCWDLHPG.
OrthoDBiEOG091G0FFH.
PhylomeDBiQ9NPB6.
TreeFamiTF312899.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000102981-MONOMER.
ReactomeiR-HSA-2173791. TGF-beta receptor signaling in EMT (epithelial to mesenchymal transition).
R-HSA-420029. Tight junction interactions.
R-HSA-4608870. Asymmetric localization of PCP proteins.
SignaLinkiQ9NPB6.
SIGNORiQ9NPB6.

Miscellaneous databases

ChiTaRSiPARD6A. human.
EvolutionaryTraceiQ9NPB6.
GeneWikiiPARD6A.
GenomeRNAii50855.
PROiQ9NPB6.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000102981.
CleanExiHS_PARD6A.
ExpressionAtlasiQ9NPB6. baseline and differential.
GenevisibleiQ9NPB6. HS.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
InterProiIPR000270. PB1_dom.
IPR001478. PDZ.
[Graphical view]
PfamiPF00564. PB1. 1 hit.
PF00595. PDZ. 1 hit.
[Graphical view]
SMARTiSM00666. PB1. 1 hit.
SM00228. PDZ. 1 hit.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 1 hit.
PROSITEiPS51745. PB1. 1 hit.
PS50106. PDZ. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPAR6A_HUMAN
AccessioniPrimary (citable) accession number: Q9NPB6
Secondary accession number(s): O14911, Q9NPJ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2003
Last sequence update: October 1, 2000
Last modified: November 30, 2016
This is version 148 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.