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Q9NPA2

- MMP25_HUMAN

UniProt

Q9NPA2 - MMP25_HUMAN

Protein

Matrix metalloproteinase-25

Gene

MMP25

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 145 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    May activate progelatinase A.

    Cofactori

    Binds 1 zinc ion per subunit.By similarity
    Calcium.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi90 – 901Zinc; in inhibited formBy similarity
    Metal bindingi233 – 2331Zinc; catalyticPROSITE-ProRule annotation
    Active sitei234 – 2341PROSITE-ProRule annotation
    Metal bindingi237 – 2371Zinc; catalyticPROSITE-ProRule annotation
    Metal bindingi243 – 2431Zinc; catalyticPROSITE-ProRule annotation

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. metalloendopeptidase activity Source: InterPro
    3. zinc ion binding Source: InterPro

    GO - Biological processi

    1. hard palate development Source: Ensembl
    2. inflammatory response Source: UniProtKB
    3. proteolysis Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase, Metalloprotease, Protease

    Keywords - Ligandi

    Calcium, Metal-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_118682. Activation of Matrix Metalloproteinases.

    Protein family/group databases

    MEROPSiM10.024.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Matrix metalloproteinase-25 (EC:3.4.24.-)
    Short name:
    MMP-25
    Alternative name(s):
    Leukolysin
    Membrane-type matrix metalloproteinase 6
    Short name:
    MT-MMP 6
    Short name:
    MTMMP6
    Membrane-type-6 matrix metalloproteinase
    Short name:
    MT6-MMP
    Short name:
    MT6MMP
    Gene namesi
    Name:MMP25
    Synonyms:MMP20, MMPL1, MT6MMP
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 16

    Organism-specific databases

    HGNCiHGNC:14246. MMP25.

    Subcellular locationi

    GO - Cellular componenti

    1. anchored component of membrane Source: UniProtKB-KW
    2. integral component of membrane Source: InterPro
    3. membrane Source: UniProtKB
    4. plasma membrane Source: UniProtKB-SubCell
    5. proteinaceous extracellular matrix Source: UniProtKB

    Keywords - Cellular componenti

    Cell membrane, Extracellular matrix, Membrane, Secreted

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA30882.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2121Sequence AnalysisAdd
    BLAST
    Propeptidei22 – 10786By similarityPRO_0000028852Add
    BLAST
    Chaini108 – 539432Matrix metalloproteinase-25PRO_0000028853Add
    BLAST
    Propeptidei540 – 56223Removed in mature formSequence AnalysisPRO_0000028854Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi317 ↔ 508By similarity
    Lipidationi539 – 5391GPI-anchor amidated alanineSequence Analysis

    Post-translational modificationi

    The precursor is cleaved by a furin endopeptidase.By similarity

    Keywords - PTMi

    Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein, Zymogen

    Proteomic databases

    PaxDbiQ9NPA2.
    PRIDEiQ9NPA2.

    PTM databases

    PhosphoSiteiQ9NPA2.

    Expressioni

    Tissue specificityi

    Expressed predominantly in leukocytes, lung and spleen. Expressed also in colon carcinoma, astrocytoma and glioblastomas.

    Gene expression databases

    BgeeiQ9NPA2.
    CleanExiHS_MMP20.
    HS_MMP25.
    GenevestigatoriQ9NPA2.

    Organism-specific databases

    HPAiCAB025177.

    Interactioni

    Protein-protein interaction databases

    BioGridi122149. 2 interactions.
    STRINGi9606.ENSP00000337816.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9NPA2.
    SMRiQ9NPA2. Positions 58-510.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati314 – 36350Hemopexin 1Add
    BLAST
    Repeati367 – 41246Hemopexin 2Add
    BLAST
    Repeati413 – 46149Hemopexin 3Add
    BLAST
    Repeati462 – 50847Hemopexin 4Add
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi88 – 958Cysteine switchBy similarity

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi103 – 1075Poly-Arg
    Compositional biasi549 – 5557Poly-Leu

    Domaini

    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

    Sequence similaritiesi

    Belongs to the peptidase M10A family.Curated
    Contains 4 hemopexin repeats.Curated

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    eggNOGiNOG295915.
    HOGENOMiHOG000217928.
    HOVERGENiHBG052484.
    InParanoidiQ9NPA2.
    KOiK08003.
    OMAiHYWRFPK.
    OrthoDBiEOG7XPZ57.
    PhylomeDBiQ9NPA2.
    TreeFamiTF315428.

    Family and domain databases

    Gene3Di2.110.10.10. 1 hit.
    3.40.390.10. 1 hit.
    InterProiIPR000585. Hemopexin-like_dom.
    IPR018487. Hemopexin-like_repeat.
    IPR024079. MetalloPept_cat_dom.
    IPR028733. MMP25.
    IPR001818. Pept_M10_metallopeptidase.
    IPR021190. Pept_M10A.
    IPR016293. Pept_M10A_stromelysin-type.
    IPR006026. Peptidase_Metallo.
    IPR002477. Peptidoglycan-bd-like.
    [Graphical view]
    PANTHERiPTHR10201:SF21. PTHR10201:SF21. 1 hit.
    PfamiPF00045. Hemopexin. 4 hits.
    PF00413. Peptidase_M10. 1 hit.
    PF01471. PG_binding_1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001191. Peptidase_M10A_matrix. 1 hit.
    PRINTSiPR00138. MATRIXIN.
    SMARTiSM00120. HX. 4 hits.
    SM00235. ZnMc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50923. SSF50923. 1 hit.
    PROSITEiPS51642. HEMOPEXIN_2. 4 hits.
    PS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9NPA2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRLRLRLLAL LLLLLAPPAR APKPSAQDVS LGVDWLTRYG YLPPPHPAQA    50
    QLQSPEKLRD AIKVMQRFAG LPETGRMDPG TVATMRKPRC SLPDVLGVAG 100
    LVRRRRRYAL SGSVWKKRTL TWRVRSFPQS SQLSQETVRV LMSYALMAWG 150
    MESGLTFHEV DSPQGQEPDI LIDFARAFHQ DSYPFDGLGG TLAHAFFPGE 200
    HPISGDTHFD DEETWTFGSK DGEGTDLFAV AVHEFGHALG LGHSSAPNSI 250
    MRPFYQGPVG DPDKYRLSQD DRDGLQQLYG KAPQTPYDKP TRKPLAPPPQ 300
    PPASPTHSPS FPIPDRCEGN FDAIANIRGE TFFFKGPWFW RLQPSGQLVS 350
    PRPARLHRFW EGLPAQVRVV QAAYARHRDG RILLFSGPQF WVFQDRQLEG 400
    GARPLTELGL PPGEEVDAVF SWPQNGKTYL VRGRQYWRYD EAAARPDPGY 450
    PRDLSLWEGA PPSPDDVTVS NAGDTYFFKG AHYWRFPKNS IKTEPDAPQP 500
    MGPNWLDCPA PSSGPRAPRP PKATPVSETC DCQCELNQAA GRWPAPIPLL 550
    LLPLLVGGVA SR 562
    Length:562
    Mass (Da):62,554
    Last modified:October 1, 2000 - v1
    Checksum:iA6A50AE05D969C64
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti47 – 471P → R in BAB20584. (PubMed:11034316)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ239053 mRNA. Translation: CAB94713.1.
    AF145442 mRNA. Translation: AAF66697.2.
    AF185270 mRNA. Translation: AAG17007.1.
    AB042328 mRNA. Translation: BAB20584.1.
    AJ272137 mRNA. Translation: CAC03490.1.
    CH471112 Genomic DNA. Translation: EAW85413.1.
    CH471112 Genomic DNA. Translation: EAW85415.1.
    CCDSiCCDS10492.1.
    RefSeqiNP_071913.1. NM_022468.4.
    UniGeneiHs.654979.

    Genome annotation databases

    EnsembliENST00000336577; ENSP00000337816; ENSG00000008516.
    GeneIDi64386.
    KEGGihsa:64386.
    UCSCiuc002cth.3. human.

    Polymorphism databases

    DMDMi12585274.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ239053 mRNA. Translation: CAB94713.1 .
    AF145442 mRNA. Translation: AAF66697.2 .
    AF185270 mRNA. Translation: AAG17007.1 .
    AB042328 mRNA. Translation: BAB20584.1 .
    AJ272137 mRNA. Translation: CAC03490.1 .
    CH471112 Genomic DNA. Translation: EAW85413.1 .
    CH471112 Genomic DNA. Translation: EAW85415.1 .
    CCDSi CCDS10492.1.
    RefSeqi NP_071913.1. NM_022468.4.
    UniGenei Hs.654979.

    3D structure databases

    ProteinModelPortali Q9NPA2.
    SMRi Q9NPA2. Positions 58-510.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 122149. 2 interactions.
    STRINGi 9606.ENSP00000337816.

    Chemistry

    BindingDBi Q9NPA2.
    ChEMBLi CHEMBL1795103.

    Protein family/group databases

    MEROPSi M10.024.

    PTM databases

    PhosphoSitei Q9NPA2.

    Polymorphism databases

    DMDMi 12585274.

    Proteomic databases

    PaxDbi Q9NPA2.
    PRIDEi Q9NPA2.

    Protocols and materials databases

    DNASUi 64386.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000336577 ; ENSP00000337816 ; ENSG00000008516 .
    GeneIDi 64386.
    KEGGi hsa:64386.
    UCSCi uc002cth.3. human.

    Organism-specific databases

    CTDi 64386.
    GeneCardsi GC16P003127.
    HGNCi HGNC:14246. MMP25.
    HPAi CAB025177.
    MIMi 608482. gene.
    neXtProti NX_Q9NPA2.
    PharmGKBi PA30882.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG295915.
    HOGENOMi HOG000217928.
    HOVERGENi HBG052484.
    InParanoidi Q9NPA2.
    KOi K08003.
    OMAi HYWRFPK.
    OrthoDBi EOG7XPZ57.
    PhylomeDBi Q9NPA2.
    TreeFami TF315428.

    Enzyme and pathway databases

    Reactomei REACT_118682. Activation of Matrix Metalloproteinases.

    Miscellaneous databases

    GeneWikii MMP25.
    GenomeRNAii 64386.
    NextBioi 66313.
    PROi Q9NPA2.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9NPA2.
    CleanExi HS_MMP20.
    HS_MMP25.
    Genevestigatori Q9NPA2.

    Family and domain databases

    Gene3Di 2.110.10.10. 1 hit.
    3.40.390.10. 1 hit.
    InterProi IPR000585. Hemopexin-like_dom.
    IPR018487. Hemopexin-like_repeat.
    IPR024079. MetalloPept_cat_dom.
    IPR028733. MMP25.
    IPR001818. Pept_M10_metallopeptidase.
    IPR021190. Pept_M10A.
    IPR016293. Pept_M10A_stromelysin-type.
    IPR006026. Peptidase_Metallo.
    IPR002477. Peptidoglycan-bd-like.
    [Graphical view ]
    PANTHERi PTHR10201:SF21. PTHR10201:SF21. 1 hit.
    Pfami PF00045. Hemopexin. 4 hits.
    PF00413. Peptidase_M10. 1 hit.
    PF01471. PG_binding_1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001191. Peptidase_M10A_matrix. 1 hit.
    PRINTSi PR00138. MATRIXIN.
    SMARTi SM00120. HX. 4 hits.
    SM00235. ZnMc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50923. SSF50923. 1 hit.
    PROSITEi PS51642. HEMOPEXIN_2. 4 hits.
    PS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Human MT6-matrix metalloproteinase: identification, progelatinase A activation, and expression in brain tumors."
      Velasco G., Cal S., Merlos-Suarez A., Ferrando A.A., Alvarez S., Nakano A., Arribas J., Lopez-Otin C.
      Cancer Res. 60:877-882(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Fetal liver.
    2. "Leukolysin/MMP25/MT6-MMP: a novel matrix metalloproteinase specifically expressed in the leukocyte lineage."
      Pei D.Q.
      Cell Res. 9:291-303(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "Membrane-type 6 matrix metalloproteinase (MT6-MMP, MMP-25) is the second glycosyl-phosphatidyl inositol (GPI)-anchored MMP."
      Kojima S., Itoh Y., Matsumoto S., Masuho Y., Seiki M.
      FEBS Lett. 480:142-146(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], GPI-ANCHOR.
    4. "Molecular cloning of a novel human membrane-type matrix metalloproteinase (MT-MMP) predominantly expressed in dendritic cells."
      de Saint-Vis B.M., Clair-Moninot V.A., Lambert C.A., Vanbervliet B., Pin J.J., Chalus L., Ait-Yahia S., Caux C., Richelle-Nusgens B.V., Fossiez F., Lebecque S.
      Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

    Entry informationi

    Entry nameiMMP25_HUMAN
    AccessioniPrimary (citable) accession number: Q9NPA2
    Secondary accession number(s): D3DUA8, Q9H3Q0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 24, 2001
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 145 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 16
      Human chromosome 16: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. Peptidase families
      Classification of peptidase families and list of entries
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3