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Protein

Matrix metalloproteinase-25

Gene

MMP25

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May activate progelatinase A.

Cofactori

Protein has several cofactor binding sites:
  • Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity
  • Ca2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi90Zinc; in inhibited formBy similarity1
Metal bindingi233Zinc; catalyticPROSITE-ProRule annotation1
Active sitei234PROSITE-ProRule annotation1
Metal bindingi237Zinc; catalyticPROSITE-ProRule annotation1
Metal bindingi243Zinc; catalyticPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • hard palate development Source: Ensembl
  • inflammatory response Source: UniProtKB
  • proteolysis Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciZFISH:ENSG00000008516-MONOMER.
ReactomeiR-HSA-1592389. Activation of Matrix Metalloproteinases.
R-HSA-6798695. Neutrophil degranulation.

Protein family/group databases

MEROPSiM10.024.

Names & Taxonomyi

Protein namesi
Recommended name:
Matrix metalloproteinase-25 (EC:3.4.24.-)
Short name:
MMP-25
Alternative name(s):
Leukolysin
Membrane-type matrix metalloproteinase 6
Short name:
MT-MMP 6
Short name:
MTMMP6
Membrane-type-6 matrix metalloproteinase
Short name:
MT6-MMP
Short name:
MT6MMP
Gene namesi
Name:MMP25
Synonyms:MMP20, MMPL1, MT6MMP
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:14246. MMP25.

Subcellular locationi

GO - Cellular componenti

  • anchored component of membrane Source: UniProtKB-KW
  • integral component of membrane Source: InterPro
  • membrane Source: UniProtKB
  • plasma membrane Source: UniProtKB-SubCell
  • proteinaceous extracellular matrix Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Extracellular matrix, Membrane, Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi64386.
OpenTargetsiENSG00000008516.
PharmGKBiPA30882.

Chemistry databases

ChEMBLiCHEMBL1795103.
DrugBankiDB00786. Marimastat.
GuidetoPHARMACOLOGYi1647.

Polymorphism and mutation databases

BioMutaiMMP25.
DMDMi12585274.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
PropeptideiPRO_000002885222 – 107By similarityAdd BLAST86
ChainiPRO_0000028853108 – 539Matrix metalloproteinase-25Add BLAST432
PropeptideiPRO_0000028854540 – 562Removed in mature formSequence analysisAdd BLAST23

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi317 ↔ 508By similarity
Lipidationi539GPI-anchor amidated alanineSequence analysis1

Post-translational modificationi

The precursor is cleaved by a furin endopeptidase.By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein, Zymogen

Proteomic databases

EPDiQ9NPA2.
PaxDbiQ9NPA2.
PRIDEiQ9NPA2.

PTM databases

iPTMnetiQ9NPA2.
PhosphoSitePlusiQ9NPA2.

Expressioni

Tissue specificityi

Expressed predominantly in leukocytes, lung and spleen. Expressed also in colon carcinoma, astrocytoma and glioblastomas.

Gene expression databases

BgeeiENSG00000008516.
CleanExiHS_MMP20.
HS_MMP25.
ExpressionAtlasiQ9NPA2. baseline and differential.
GenevisibleiQ9NPA2. HS.

Organism-specific databases

HPAiCAB025177.

Interactioni

Protein-protein interaction databases

BioGridi122149. 1 interactor.
IntActiQ9NPA2. 1 interactor.
STRINGi9606.ENSP00000337816.

Chemistry databases

BindingDBiQ9NPA2.

Structurei

3D structure databases

ProteinModelPortaliQ9NPA2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati314 – 363Hemopexin 1Add BLAST50
Repeati367 – 412Hemopexin 2Add BLAST46
Repeati413 – 461Hemopexin 3Add BLAST49
Repeati462 – 508Hemopexin 4Add BLAST47

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi88 – 95Cysteine switchBy similarity8

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi103 – 107Poly-Arg5
Compositional biasi549 – 555Poly-Leu7

Domaini

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Sequence similaritiesi

Belongs to the peptidase M10A family.Curated
Contains 4 hemopexin repeats.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG1565. Eukaryota.
ENOG410XQ5D. LUCA.
GeneTreeiENSGT00760000118870.
HOGENOMiHOG000217928.
HOVERGENiHBG052484.
InParanoidiQ9NPA2.
KOiK08003.
OMAiHYWRFPK.
OrthoDBiEOG091G03DP.
PhylomeDBiQ9NPA2.
TreeFamiTF315428.

Family and domain databases

CDDicd00094. HX. 1 hit.
cd04278. ZnMc_MMP. 1 hit.
Gene3Di2.110.10.10. 1 hit.
3.40.390.10. 1 hit.
InterProiIPR000585. Hemopexin-like_dom.
IPR018487. Hemopexin-like_repeat.
IPR033739. M10A_MMP.
IPR024079. MetalloPept_cat_dom.
IPR028733. MMP25.
IPR001818. Pept_M10_metallopeptidase.
IPR021190. Pept_M10A.
IPR016293. Pept_M10A_stromelysin-type.
IPR006026. Peptidase_Metallo.
IPR002477. Peptidoglycan-bd-like.
[Graphical view]
PANTHERiPTHR10201:SF142. PTHR10201:SF142. 1 hit.
PfamiPF00045. Hemopexin. 4 hits.
PF00413. Peptidase_M10. 1 hit.
PF01471. PG_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF001191. Peptidase_M10A_matrix. 1 hit.
PRINTSiPR00138. MATRIXIN.
SMARTiSM00120. HX. 4 hits.
SM00235. ZnMc. 1 hit.
[Graphical view]
SUPFAMiSSF50923. SSF50923. 1 hit.
PROSITEiPS51642. HEMOPEXIN_2. 4 hits.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9NPA2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLRLRLLAL LLLLLAPPAR APKPSAQDVS LGVDWLTRYG YLPPPHPAQA
60 70 80 90 100
QLQSPEKLRD AIKVMQRFAG LPETGRMDPG TVATMRKPRC SLPDVLGVAG
110 120 130 140 150
LVRRRRRYAL SGSVWKKRTL TWRVRSFPQS SQLSQETVRV LMSYALMAWG
160 170 180 190 200
MESGLTFHEV DSPQGQEPDI LIDFARAFHQ DSYPFDGLGG TLAHAFFPGE
210 220 230 240 250
HPISGDTHFD DEETWTFGSK DGEGTDLFAV AVHEFGHALG LGHSSAPNSI
260 270 280 290 300
MRPFYQGPVG DPDKYRLSQD DRDGLQQLYG KAPQTPYDKP TRKPLAPPPQ
310 320 330 340 350
PPASPTHSPS FPIPDRCEGN FDAIANIRGE TFFFKGPWFW RLQPSGQLVS
360 370 380 390 400
PRPARLHRFW EGLPAQVRVV QAAYARHRDG RILLFSGPQF WVFQDRQLEG
410 420 430 440 450
GARPLTELGL PPGEEVDAVF SWPQNGKTYL VRGRQYWRYD EAAARPDPGY
460 470 480 490 500
PRDLSLWEGA PPSPDDVTVS NAGDTYFFKG AHYWRFPKNS IKTEPDAPQP
510 520 530 540 550
MGPNWLDCPA PSSGPRAPRP PKATPVSETC DCQCELNQAA GRWPAPIPLL
560
LLPLLVGGVA SR
Length:562
Mass (Da):62,554
Last modified:October 1, 2000 - v1
Checksum:iA6A50AE05D969C64
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti47P → R in BAB20584 (PubMed:11034316).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ239053 mRNA. Translation: CAB94713.1.
AF145442 mRNA. Translation: AAF66697.2.
AF185270 mRNA. Translation: AAG17007.1.
AB042328 mRNA. Translation: BAB20584.1.
AJ272137 mRNA. Translation: CAC03490.1.
CH471112 Genomic DNA. Translation: EAW85413.1.
CH471112 Genomic DNA. Translation: EAW85415.1.
CCDSiCCDS10492.1.
RefSeqiNP_071913.1. NM_022468.4.
UniGeneiHs.654979.

Genome annotation databases

EnsembliENST00000336577; ENSP00000337816; ENSG00000008516.
GeneIDi64386.
KEGGihsa:64386.
UCSCiuc002cth.4. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ239053 mRNA. Translation: CAB94713.1.
AF145442 mRNA. Translation: AAF66697.2.
AF185270 mRNA. Translation: AAG17007.1.
AB042328 mRNA. Translation: BAB20584.1.
AJ272137 mRNA. Translation: CAC03490.1.
CH471112 Genomic DNA. Translation: EAW85413.1.
CH471112 Genomic DNA. Translation: EAW85415.1.
CCDSiCCDS10492.1.
RefSeqiNP_071913.1. NM_022468.4.
UniGeneiHs.654979.

3D structure databases

ProteinModelPortaliQ9NPA2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122149. 1 interactor.
IntActiQ9NPA2. 1 interactor.
STRINGi9606.ENSP00000337816.

Chemistry databases

BindingDBiQ9NPA2.
ChEMBLiCHEMBL1795103.
DrugBankiDB00786. Marimastat.
GuidetoPHARMACOLOGYi1647.

Protein family/group databases

MEROPSiM10.024.

PTM databases

iPTMnetiQ9NPA2.
PhosphoSitePlusiQ9NPA2.

Polymorphism and mutation databases

BioMutaiMMP25.
DMDMi12585274.

Proteomic databases

EPDiQ9NPA2.
PaxDbiQ9NPA2.
PRIDEiQ9NPA2.

Protocols and materials databases

DNASUi64386.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000336577; ENSP00000337816; ENSG00000008516.
GeneIDi64386.
KEGGihsa:64386.
UCSCiuc002cth.4. human.

Organism-specific databases

CTDi64386.
DisGeNETi64386.
GeneCardsiMMP25.
HGNCiHGNC:14246. MMP25.
HPAiCAB025177.
MIMi608482. gene.
neXtProtiNX_Q9NPA2.
OpenTargetsiENSG00000008516.
PharmGKBiPA30882.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1565. Eukaryota.
ENOG410XQ5D. LUCA.
GeneTreeiENSGT00760000118870.
HOGENOMiHOG000217928.
HOVERGENiHBG052484.
InParanoidiQ9NPA2.
KOiK08003.
OMAiHYWRFPK.
OrthoDBiEOG091G03DP.
PhylomeDBiQ9NPA2.
TreeFamiTF315428.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000008516-MONOMER.
ReactomeiR-HSA-1592389. Activation of Matrix Metalloproteinases.
R-HSA-6798695. Neutrophil degranulation.

Miscellaneous databases

GeneWikiiMMP25.
GenomeRNAii64386.
PROiQ9NPA2.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000008516.
CleanExiHS_MMP20.
HS_MMP25.
ExpressionAtlasiQ9NPA2. baseline and differential.
GenevisibleiQ9NPA2. HS.

Family and domain databases

CDDicd00094. HX. 1 hit.
cd04278. ZnMc_MMP. 1 hit.
Gene3Di2.110.10.10. 1 hit.
3.40.390.10. 1 hit.
InterProiIPR000585. Hemopexin-like_dom.
IPR018487. Hemopexin-like_repeat.
IPR033739. M10A_MMP.
IPR024079. MetalloPept_cat_dom.
IPR028733. MMP25.
IPR001818. Pept_M10_metallopeptidase.
IPR021190. Pept_M10A.
IPR016293. Pept_M10A_stromelysin-type.
IPR006026. Peptidase_Metallo.
IPR002477. Peptidoglycan-bd-like.
[Graphical view]
PANTHERiPTHR10201:SF142. PTHR10201:SF142. 1 hit.
PfamiPF00045. Hemopexin. 4 hits.
PF00413. Peptidase_M10. 1 hit.
PF01471. PG_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF001191. Peptidase_M10A_matrix. 1 hit.
PRINTSiPR00138. MATRIXIN.
SMARTiSM00120. HX. 4 hits.
SM00235. ZnMc. 1 hit.
[Graphical view]
SUPFAMiSSF50923. SSF50923. 1 hit.
PROSITEiPS51642. HEMOPEXIN_2. 4 hits.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMMP25_HUMAN
AccessioniPrimary (citable) accession number: Q9NPA2
Secondary accession number(s): D3DUA8, Q9H3Q0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: October 1, 2000
Last modified: November 30, 2016
This is version 162 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.