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Q9NP97

- DLRB1_HUMAN

UniProt

Q9NP97 - DLRB1_HUMAN

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Protein

Dynein light chain roadblock-type 1

Gene

DYNLRB1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules.

GO - Molecular functioni

  1. microtubule motor activity Source: UniProtKB

GO - Biological processi

  1. metabolic process Source: GOC
  2. microtubule-based movement Source: UniProtKB
  3. transport Source: UniProtKB-KW
  4. visual behavior Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Motor protein

Keywords - Biological processi

Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Dynein light chain roadblock-type 1
Alternative name(s):
Bithoraxoid-like protein
Short name:
BLP
Dynein light chain 2A, cytoplasmic
Dynein-associated protein Km23
Roadblock domain-containing protein 1
Gene namesi
Name:DYNLRB1
Synonyms:BITH, DNCL2A, DNLC2A, ROBLD1
ORF Names:HSPC162
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 20

Organism-specific databases

HGNCiHGNC:15468. DYNLRB1.

Subcellular locationi

GO - Cellular componenti

  1. centrosome Source: UniProtKB
  2. cytoplasm Source: UniProtKB
  3. cytoplasmic dynein complex Source: UniProtKB
  4. membrane Source: UniProtKB
  5. microtubule Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Dynein, Microtubule

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA27436.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 9695Dynein light chain roadblock-type 1PRO_0000220955Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ9NP97.
PaxDbiQ9NP97.
PRIDEiQ9NP97.

PTM databases

PhosphoSiteiQ9NP97.

Expressioni

Tissue specificityi

High expression in heart, liver, brain and pancreas; moderate in placenta, skeletal muscle and kidney; low in lung, prostate, testis, small intestine and colon. Isoform 1 expression is up-regulated in 64% hepatocellular carcinoma (HCC) patients.1 Publication

Gene expression databases

BgeeiQ9NP97.
CleanExiHS_DYNLRB1.
ExpressionAtlasiQ9NP97. baseline and differential.
GenevestigatoriQ9NP97.

Organism-specific databases

HPAiCAB009845.

Interactioni

Subunit structurei

Homodimer. The cytoplasmic dynein 1 complex consists of two catalytic heavy chains (HCs) and a number of non-catalytic subunits presented by intermediate chains (ICs), light intermediate chains (LICs) and light chains (LCs); the composition seems to vary in respect to the IC, LIC and LC composition. The heavy chain homodimer serves as a scaffold for the probable homodimeric assembly of the respective non-catalytic subunits. The ICs and LICs bind directly to the HC dimer and the LCs assemble on the IC dimer. Self-associates. Interacts with DYNLRB2. Interacts with DYNC1I1 and DYNC1I2. Interacts with RAB6A isoform 1 (GTP-bound); the interaction is direct. Interacts with RAB6A isoform 2 (GDP-bound); the interaction is direct. Interacts with RAB6B (GDP-bound).4 Publications

Protein-protein interaction databases

BioGridi123715. 22 interactions.
IntActiQ9NP97. 10 interactions.
MINTiMINT-1485399.
STRINGi9606.ENSP00000349679.

Structurei

Secondary structure

1
96
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi6 – 127Combined sources
Beta strandi17 – 237Combined sources
Beta strandi25 – 273Combined sources
Beta strandi29 – 346Combined sources
Helixi36 – 6025Combined sources
Beta strandi66 – 7510Combined sources
Beta strandi77 – 815Combined sources
Beta strandi87 – 926Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1Z09NMR-A/B1-96[»]
2B95NMR-A/B1-96[»]
2E8JNMR-A/B1-96[»]
2HZ5X-ray2.10A/B1-96[»]
ProteinModelPortaliQ9NP97.
SMRiQ9NP97. Positions 1-96.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9NP97.

Family & Domainsi

Sequence similaritiesi

Belongs to the GAMAD family.Curated

Phylogenomic databases

eggNOGiNOG309619.
GeneTreeiENSGT00390000011067.
HOGENOMiHOG000232033.
HOVERGENiHBG058598.
InParanoidiQ9NP97.
KOiK10419.
OMAiQKEARQH.
OrthoDBiEOG7X0VKJ.
PhylomeDBiQ9NP97.
TreeFamiTF315165.

Family and domain databases

InterProiIPR004942. Dynein_light-rel.
IPR016561. Dynein_light_roadblock-type.
[Graphical view]
PfamiPF03259. Robl_LC7. 1 hit.
[Graphical view]
PIRSFiPIRSF009998. DLC7. 1 hit.
SMARTiSM00960. Robl_LC7. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Note: Additional isoforms seem to exist.

Isoform 1 (identifier: Q9NP97-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAEVEETLKR LQSQKGVQGI IVVNTEGIPI KSTMDNPTTT QYASLMHSFI
60 70 80 90
LKARSTVRDI DPQNDLTFLR IRSKKNEIMV APDKDYFLIV IQNPTE
Length:96
Mass (Da):10,922
Last modified:January 23, 2007 - v3
Checksum:iAAE1EFCD372897B3
GO
Isoform 2 (identifier: Q9NP97-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     28-47: IPIKSTMDNPTTTQYASLMH → GWEPLGHCGDRSRPPAQGCP
     48-96: Missing.

Note: May result from the retention of an intron in the cDNA.

Show »
Length:47
Mass (Da):5,060
Checksum:i17421DC72701A8A2
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti13 – 131S → R.
Corresponds to variant rs1063616 [ dbSNP | Ensembl ].
VAR_049124
Natural varianti71 – 711I → F.
Corresponds to variant rs10036 [ dbSNP | Ensembl ].
VAR_049125

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei28 – 4720IPIKS…ASLMH → GWEPLGHCGDRSRPPAQGCP in isoform 2. 1 PublicationVSP_007236Add
BLAST
Alternative sequencei48 – 9649Missing in isoform 2. 1 PublicationVSP_007237Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF132750 mRNA. Translation: AAL75951.1.
AF165516 mRNA. Translation: AAF86646.1.
AF178431 mRNA. Translation: AAK95342.1.
AY026513 mRNA. Translation: AAK18712.1.
AF161511 mRNA. Translation: AAF29126.1.
AL109923, AL136173 Genomic DNA. Translation: CAI21456.1.
AL136173, AL109923 Genomic DNA. Translation: CAI23308.1.
AL109923, AL136173 Genomic DNA. Translation: CAM28250.1.
AL136173, AL109923 Genomic DNA. Translation: CAM28343.1.
BC002481 mRNA. Translation: AAH02481.1.
BC007223 mRNA. No translation available.
CCDSiCCDS13235.1. [Q9NP97-1]
RefSeqiNP_001268656.1. NM_001281727.1.
NP_001268657.1. NM_001281728.1.
NP_001268658.1. NM_001281729.1.
NP_054902.1. NM_014183.3. [Q9NP97-1]
NP_808852.1. NM_177953.2.
UniGeneiHs.593920.

Genome annotation databases

EnsembliENST00000300469; ENSP00000300469; ENSG00000125971. [Q9NP97-2]
ENST00000357156; ENSP00000349679; ENSG00000125971. [Q9NP97-1]
GeneIDi83658.
KEGGihsa:83658.
UCSCiuc002xal.3. human. [Q9NP97-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF132750 mRNA. Translation: AAL75951.1 .
AF165516 mRNA. Translation: AAF86646.1 .
AF178431 mRNA. Translation: AAK95342.1 .
AY026513 mRNA. Translation: AAK18712.1 .
AF161511 mRNA. Translation: AAF29126.1 .
AL109923 , AL136173 Genomic DNA. Translation: CAI21456.1 .
AL136173 , AL109923 Genomic DNA. Translation: CAI23308.1 .
AL109923 , AL136173 Genomic DNA. Translation: CAM28250.1 .
AL136173 , AL109923 Genomic DNA. Translation: CAM28343.1 .
BC002481 mRNA. Translation: AAH02481.1 .
BC007223 mRNA. No translation available.
CCDSi CCDS13235.1. [Q9NP97-1 ]
RefSeqi NP_001268656.1. NM_001281727.1.
NP_001268657.1. NM_001281728.1.
NP_001268658.1. NM_001281729.1.
NP_054902.1. NM_014183.3. [Q9NP97-1 ]
NP_808852.1. NM_177953.2.
UniGenei Hs.593920.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1Z09 NMR - A/B 1-96 [» ]
2B95 NMR - A/B 1-96 [» ]
2E8J NMR - A/B 1-96 [» ]
2HZ5 X-ray 2.10 A/B 1-96 [» ]
ProteinModelPortali Q9NP97.
SMRi Q9NP97. Positions 1-96.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 123715. 22 interactions.
IntActi Q9NP97. 10 interactions.
MINTi MINT-1485399.
STRINGi 9606.ENSP00000349679.

PTM databases

PhosphoSitei Q9NP97.

Proteomic databases

MaxQBi Q9NP97.
PaxDbi Q9NP97.
PRIDEi Q9NP97.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000300469 ; ENSP00000300469 ; ENSG00000125971 . [Q9NP97-2 ]
ENST00000357156 ; ENSP00000349679 ; ENSG00000125971 . [Q9NP97-1 ]
GeneIDi 83658.
KEGGi hsa:83658.
UCSCi uc002xal.3. human. [Q9NP97-1 ]

Organism-specific databases

CTDi 83658.
GeneCardsi GC20P033104.
HGNCi HGNC:15468. DYNLRB1.
HPAi CAB009845.
MIMi 607167. gene.
neXtProti NX_Q9NP97.
PharmGKBi PA27436.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG309619.
GeneTreei ENSGT00390000011067.
HOGENOMi HOG000232033.
HOVERGENi HBG058598.
InParanoidi Q9NP97.
KOi K10419.
OMAi QKEARQH.
OrthoDBi EOG7X0VKJ.
PhylomeDBi Q9NP97.
TreeFami TF315165.

Miscellaneous databases

ChiTaRSi DYNLRB1. human.
EvolutionaryTracei Q9NP97.
GeneWikii DYNLRB1.
GenomeRNAii 83658.
NextBioi 72623.
PROi Q9NP97.
SOURCEi Search...

Gene expression databases

Bgeei Q9NP97.
CleanExi HS_DYNLRB1.
ExpressionAtlasi Q9NP97. baseline and differential.
Genevestigatori Q9NP97.

Family and domain databases

InterProi IPR004942. Dynein_light-rel.
IPR016561. Dynein_light_roadblock-type.
[Graphical view ]
Pfami PF03259. Robl_LC7. 1 hit.
[Graphical view ]
PIRSFi PIRSF009998. DLC7. 1 hit.
SMARTi SM00960. Robl_LC7. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of two novel human dynein light chain genes, DNLC2A and DNLC2B, and their expression changes in hepatocellular carcinoma tissues from 68 Chinese patients."
    Jiang J., Yu L., Huang X., Chen X., Li D., Zhang Y., Tang L., Zhao S.
    Gene 281:103-113(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
    Tissue: Liver and Testis.
  2. "Novel genes expressed in hematopoietic stem/progenitor cells from myelodysplastic syndrome patients."
    Gu J., Huang Q., Yu Y., Xu S., Han Z., Fu G., Zhou J., Wang Y., Huang C., Ren S., Tu Y., Chen Z.
    Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Hematopoietic stem cell.
  3. "BitH, a human homolog of bithorax Drosophila melanogaster gene, on chromosome 20q."
    Fracchiolla N.S., Cortelezzi A., Lambertenghi-Deliliers G.
    Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  4. "Km23: role in growth factor signaling."
    Tang Q., Staub C.M., Mulder K.M.
    Submitted (JAN-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Placenta.
  5. "Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells."
    Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G., Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W., Tao J., Huang Q.-H., Zhou J., Hu G.-X.
    , Gu J., Chen S.-J., Chen Z.
    Genome Res. 10:1546-1560(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Umbilical cord blood.
  6. "The DNA sequence and comparative analysis of human chromosome 20."
    Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E.
    , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.
    Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Kidney and Lymph.
  8. "Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides."
    Gevaert K., Goethals M., Martens L., Van Damme J., Staes A., Thomas G.R., Vandekerckhove J.
    Nat. Biotechnol. 21:566-569(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-10.
    Tissue: Platelet.
  9. Lubec G., Chen W.-Q., Sun Y.
    Submitted (DEC-2008) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 16-52 AND 59-70, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Fetal brain cortex.
  10. "The roadblock light chain binds a novel region of the cytoplasmic Dynein intermediate chain."
    Susalka S.J., Nikulina K., Salata M.W., Vaughan P.S., King S.M., Vaughan K.T., Pfister K.K.
    J. Biol. Chem. 277:32939-32946(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH DYNC1I1 AND DYNC1I2.
  11. "Rab6 family proteins interact with the dynein light chain protein DYNLRB1."
    Wanschers B.F.J., van de Vorstenbosch R., Wijers M., Wieringa B., King S.M., Fransen J.
    Cell Motil. Cytoskeleton 65:183-196(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RAP6A AND RAP6B.
  12. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. Cited for: STRUCTURE BY NMR, SUBUNIT.
  14. "Crystal structure of human dynein light chain Dnlc2A: structural insights into the interaction with IC74."
    Liu J.F., Wang Z.X., Wang X.Q., Tang Q., An X.M., Gui L.L., Liang D.C.
    Biochem. Biophys. Res. Commun. 349:1125-1129(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS), SUBUNIT.

Entry informationi

Entry nameiDLRB1_HUMAN
AccessioniPrimary (citable) accession number: Q9NP97
Secondary accession number(s): B1AKR5
, Q5TC72, Q96IV3, Q9NQM2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 23, 2003
Last sequence update: January 23, 2007
Last modified: November 26, 2014
This is version 130 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3