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Q9NP97

- DLRB1_HUMAN

UniProt

Q9NP97 - DLRB1_HUMAN

Protein

Dynein light chain roadblock-type 1

Gene

DYNLRB1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules.

    GO - Molecular functioni

    1. microtubule motor activity Source: UniProtKB

    GO - Biological processi

    1. metabolic process Source: GOC
    2. microtubule-based movement Source: UniProtKB
    3. transport Source: UniProtKB-KW
    4. visual behavior Source: UniProtKB

    Keywords - Molecular functioni

    Motor protein

    Keywords - Biological processi

    Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dynein light chain roadblock-type 1
    Alternative name(s):
    Bithoraxoid-like protein
    Short name:
    BLP
    Dynein light chain 2A, cytoplasmic
    Dynein-associated protein Km23
    Roadblock domain-containing protein 1
    Gene namesi
    Name:DYNLRB1
    Synonyms:BITH, DNCL2A, DNLC2A, ROBLD1
    ORF Names:HSPC162
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 20

    Organism-specific databases

    HGNCiHGNC:15468. DYNLRB1.

    Subcellular locationi

    GO - Cellular componenti

    1. centrosome Source: UniProtKB
    2. cytoplasm Source: UniProtKB
    3. cytoplasmic dynein complex Source: UniProtKB
    4. membrane Source: UniProtKB
    5. microtubule Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton, Dynein, Microtubule

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA27436.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 9695Dynein light chain roadblock-type 1PRO_0000220955Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiQ9NP97.
    PaxDbiQ9NP97.
    PRIDEiQ9NP97.

    PTM databases

    PhosphoSiteiQ9NP97.

    Expressioni

    Tissue specificityi

    High expression in heart, liver, brain and pancreas; moderate in placenta, skeletal muscle and kidney; low in lung, prostate, testis, small intestine and colon. Isoform 1 expression is up-regulated in 64% hepatocellular carcinoma (HCC) patients.1 Publication

    Gene expression databases

    ArrayExpressiQ9NP97.
    BgeeiQ9NP97.
    CleanExiHS_DYNLRB1.
    GenevestigatoriQ9NP97.

    Organism-specific databases

    HPAiCAB009845.

    Interactioni

    Subunit structurei

    Homodimer. The cytoplasmic dynein 1 complex consists of two catalytic heavy chains (HCs) and a number of non-catalytic subunits presented by intermediate chains (ICs), light intermediate chains (LICs) and light chains (LCs); the composition seems to vary in respect to the IC, LIC and LC composition. The heavy chain homodimer serves as a scaffold for the probable homodimeric assembly of the respective non-catalytic subunits. The ICs and LICs bind directly to the HC dimer and the LCs assemble on the IC dimer. Self-associates. Interacts with DYNLRB2. Interacts with DYNC1I1 and DYNC1I2. Interacts with RAB6A isoform 1 (GTP-bound); the interaction is direct. Interacts with RAB6A isoform 2 (GDP-bound); the interaction is direct. Interacts with RAB6B (GDP-bound).4 Publications

    Protein-protein interaction databases

    BioGridi123715. 22 interactions.
    IntActiQ9NP97. 10 interactions.
    MINTiMINT-1485399.
    STRINGi9606.ENSP00000349679.

    Structurei

    Secondary structure

    1
    96
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi6 – 127
    Beta strandi17 – 237
    Beta strandi25 – 273
    Beta strandi29 – 346
    Helixi36 – 6025
    Beta strandi66 – 7510
    Beta strandi77 – 815
    Beta strandi87 – 926

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1Z09NMR-A/B1-96[»]
    2B95NMR-A/B1-96[»]
    2E8JNMR-A/B1-96[»]
    2HZ5X-ray2.10A/B1-96[»]
    ProteinModelPortaliQ9NP97.
    SMRiQ9NP97. Positions 1-96.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9NP97.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the GAMAD family.Curated

    Phylogenomic databases

    eggNOGiNOG309619.
    HOGENOMiHOG000232033.
    HOVERGENiHBG058598.
    KOiK10419.
    OMAiQKEARQH.
    OrthoDBiEOG7X0VKJ.
    PhylomeDBiQ9NP97.
    TreeFamiTF315165.

    Family and domain databases

    InterProiIPR004942. Dynein_light-rel.
    IPR016561. Dynein_light_roadblock-type.
    [Graphical view]
    PfamiPF03259. Robl_LC7. 1 hit.
    [Graphical view]
    PIRSFiPIRSF009998. DLC7. 1 hit.
    SMARTiSM00960. Robl_LC7. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Note: Additional isoforms seem to exist.

    Isoform 1 (identifier: Q9NP97-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAEVEETLKR LQSQKGVQGI IVVNTEGIPI KSTMDNPTTT QYASLMHSFI   50
    LKARSTVRDI DPQNDLTFLR IRSKKNEIMV APDKDYFLIV IQNPTE 96
    Length:96
    Mass (Da):10,922
    Last modified:January 23, 2007 - v3
    Checksum:iAAE1EFCD372897B3
    GO
    Isoform 2 (identifier: Q9NP97-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         28-47: IPIKSTMDNPTTTQYASLMH → GWEPLGHCGDRSRPPAQGCP
         48-96: Missing.

    Note: May result from the retention of an intron in the cDNA.

    Show »
    Length:47
    Mass (Da):5,060
    Checksum:i17421DC72701A8A2
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti13 – 131S → R.
    Corresponds to variant rs1063616 [ dbSNP | Ensembl ].
    VAR_049124
    Natural varianti71 – 711I → F.
    Corresponds to variant rs10036 [ dbSNP | Ensembl ].
    VAR_049125

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei28 – 4720IPIKS…ASLMH → GWEPLGHCGDRSRPPAQGCP in isoform 2. 1 PublicationVSP_007236Add
    BLAST
    Alternative sequencei48 – 9649Missing in isoform 2. 1 PublicationVSP_007237Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF132750 mRNA. Translation: AAL75951.1.
    AF165516 mRNA. Translation: AAF86646.1.
    AF178431 mRNA. Translation: AAK95342.1.
    AY026513 mRNA. Translation: AAK18712.1.
    AF161511 mRNA. Translation: AAF29126.1.
    AL109923, AL136173 Genomic DNA. Translation: CAI21456.1.
    AL136173, AL109923 Genomic DNA. Translation: CAI23308.1.
    AL109923, AL136173 Genomic DNA. Translation: CAM28250.1.
    AL136173, AL109923 Genomic DNA. Translation: CAM28343.1.
    BC002481 mRNA. Translation: AAH02481.1.
    BC007223 mRNA. No translation available.
    CCDSiCCDS13235.1. [Q9NP97-1]
    RefSeqiNP_001268656.1. NM_001281727.1.
    NP_001268657.1. NM_001281728.1.
    NP_001268658.1. NM_001281729.1.
    NP_054902.1. NM_014183.3. [Q9NP97-1]
    NP_808852.1. NM_177953.2.
    UniGeneiHs.593920.

    Genome annotation databases

    EnsembliENST00000300469; ENSP00000300469; ENSG00000125971. [Q9NP97-2]
    ENST00000357156; ENSP00000349679; ENSG00000125971. [Q9NP97-1]
    GeneIDi83658.
    KEGGihsa:83658.
    UCSCiuc002xal.3. human. [Q9NP97-1]

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF132750 mRNA. Translation: AAL75951.1 .
    AF165516 mRNA. Translation: AAF86646.1 .
    AF178431 mRNA. Translation: AAK95342.1 .
    AY026513 mRNA. Translation: AAK18712.1 .
    AF161511 mRNA. Translation: AAF29126.1 .
    AL109923 , AL136173 Genomic DNA. Translation: CAI21456.1 .
    AL136173 , AL109923 Genomic DNA. Translation: CAI23308.1 .
    AL109923 , AL136173 Genomic DNA. Translation: CAM28250.1 .
    AL136173 , AL109923 Genomic DNA. Translation: CAM28343.1 .
    BC002481 mRNA. Translation: AAH02481.1 .
    BC007223 mRNA. No translation available.
    CCDSi CCDS13235.1. [Q9NP97-1 ]
    RefSeqi NP_001268656.1. NM_001281727.1.
    NP_001268657.1. NM_001281728.1.
    NP_001268658.1. NM_001281729.1.
    NP_054902.1. NM_014183.3. [Q9NP97-1 ]
    NP_808852.1. NM_177953.2.
    UniGenei Hs.593920.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1Z09 NMR - A/B 1-96 [» ]
    2B95 NMR - A/B 1-96 [» ]
    2E8J NMR - A/B 1-96 [» ]
    2HZ5 X-ray 2.10 A/B 1-96 [» ]
    ProteinModelPortali Q9NP97.
    SMRi Q9NP97. Positions 1-96.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 123715. 22 interactions.
    IntActi Q9NP97. 10 interactions.
    MINTi MINT-1485399.
    STRINGi 9606.ENSP00000349679.

    PTM databases

    PhosphoSitei Q9NP97.

    Proteomic databases

    MaxQBi Q9NP97.
    PaxDbi Q9NP97.
    PRIDEi Q9NP97.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000300469 ; ENSP00000300469 ; ENSG00000125971 . [Q9NP97-2 ]
    ENST00000357156 ; ENSP00000349679 ; ENSG00000125971 . [Q9NP97-1 ]
    GeneIDi 83658.
    KEGGi hsa:83658.
    UCSCi uc002xal.3. human. [Q9NP97-1 ]

    Organism-specific databases

    CTDi 83658.
    GeneCardsi GC20P033104.
    HGNCi HGNC:15468. DYNLRB1.
    HPAi CAB009845.
    MIMi 607167. gene.
    neXtProti NX_Q9NP97.
    PharmGKBi PA27436.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG309619.
    HOGENOMi HOG000232033.
    HOVERGENi HBG058598.
    KOi K10419.
    OMAi QKEARQH.
    OrthoDBi EOG7X0VKJ.
    PhylomeDBi Q9NP97.
    TreeFami TF315165.

    Miscellaneous databases

    ChiTaRSi DYNLRB1. human.
    EvolutionaryTracei Q9NP97.
    GeneWikii DYNLRB1.
    GenomeRNAii 83658.
    NextBioi 72623.
    PROi Q9NP97.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9NP97.
    Bgeei Q9NP97.
    CleanExi HS_DYNLRB1.
    Genevestigatori Q9NP97.

    Family and domain databases

    InterProi IPR004942. Dynein_light-rel.
    IPR016561. Dynein_light_roadblock-type.
    [Graphical view ]
    Pfami PF03259. Robl_LC7. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF009998. DLC7. 1 hit.
    SMARTi SM00960. Robl_LC7. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of two novel human dynein light chain genes, DNLC2A and DNLC2B, and their expression changes in hepatocellular carcinoma tissues from 68 Chinese patients."
      Jiang J., Yu L., Huang X., Chen X., Li D., Zhang Y., Tang L., Zhao S.
      Gene 281:103-113(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
      Tissue: Liver and Testis.
    2. "Novel genes expressed in hematopoietic stem/progenitor cells from myelodysplastic syndrome patients."
      Gu J., Huang Q., Yu Y., Xu S., Han Z., Fu G., Zhou J., Wang Y., Huang C., Ren S., Tu Y., Chen Z.
      Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Hematopoietic stem cell.
    3. "BitH, a human homolog of bithorax Drosophila melanogaster gene, on chromosome 20q."
      Fracchiolla N.S., Cortelezzi A., Lambertenghi-Deliliers G.
      Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    4. "Km23: role in growth factor signaling."
      Tang Q., Staub C.M., Mulder K.M.
      Submitted (JAN-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Tissue: Placenta.
    5. "Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells."
      Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G., Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W., Tao J., Huang Q.-H., Zhou J., Hu G.-X.
      , Gu J., Chen S.-J., Chen Z.
      Genome Res. 10:1546-1560(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Umbilical cord blood.
    6. "The DNA sequence and comparative analysis of human chromosome 20."
      Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E.
      , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.
      Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Tissue: Kidney and Lymph.
    8. "Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides."
      Gevaert K., Goethals M., Martens L., Van Damme J., Staes A., Thomas G.R., Vandekerckhove J.
      Nat. Biotechnol. 21:566-569(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-10.
      Tissue: Platelet.
    9. Lubec G., Chen W.-Q., Sun Y.
      Submitted (DEC-2008) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 16-52 AND 59-70, IDENTIFICATION BY MASS SPECTROMETRY.
      Tissue: Fetal brain cortex.
    10. "The roadblock light chain binds a novel region of the cytoplasmic Dynein intermediate chain."
      Susalka S.J., Nikulina K., Salata M.W., Vaughan P.S., King S.M., Vaughan K.T., Pfister K.K.
      J. Biol. Chem. 277:32939-32946(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH DYNC1I1 AND DYNC1I2.
    11. "Rab6 family proteins interact with the dynein light chain protein DYNLRB1."
      Wanschers B.F.J., van de Vorstenbosch R., Wijers M., Wieringa B., King S.M., Fransen J.
      Cell Motil. Cytoskeleton 65:183-196(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH RAP6A AND RAP6B.
    12. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    13. Cited for: STRUCTURE BY NMR, SUBUNIT.
    14. "Crystal structure of human dynein light chain Dnlc2A: structural insights into the interaction with IC74."
      Liu J.F., Wang Z.X., Wang X.Q., Tang Q., An X.M., Gui L.L., Liang D.C.
      Biochem. Biophys. Res. Commun. 349:1125-1129(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS), SUBUNIT.

    Entry informationi

    Entry nameiDLRB1_HUMAN
    AccessioniPrimary (citable) accession number: Q9NP97
    Secondary accession number(s): B1AKR5
    , Q5TC72, Q96IV3, Q9NQM2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 23, 2003
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 128 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 20
      Human chromosome 20: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3