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Q9NP97 (DLRB1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 127. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dynein light chain roadblock-type 1
Alternative name(s):
Bithoraxoid-like protein
Short name=BLP
Dynein light chain 2A, cytoplasmic
Dynein-associated protein Km23
Roadblock domain-containing protein 1
Gene names
Name:DYNLRB1
Synonyms:BITH, DNCL2A, DNLC2A, ROBLD1
ORF Names:HSPC162
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length96 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules.

Subunit structure

Homodimer. The cytoplasmic dynein 1 complex consists of two catalytic heavy chains (HCs) and a number of non-catalytic subunits presented by intermediate chains (ICs), light intermediate chains (LICs) and light chains (LCs); the composition seems to vary in respect to the IC, LIC and LC composition. The heavy chain homodimer serves as a scaffold for the probable homodimeric assembly of the respective non-catalytic subunits. The ICs and LICs bind directly to the HC dimer and the LCs assemble on the IC dimer. Self-associates. Interacts with DYNLRB2. Interacts with DYNC1I1 and DYNC1I2. Interacts with RAB6A isoform 1 (GTP-bound); the interaction is direct. Interacts with RAB6A isoform 2 (GDP-bound); the interaction is direct. Interacts with RAB6B (GDP-bound). Ref.10 Ref.11 Ref.13 Ref.14

Subcellular location

Cytoplasmcytoskeleton.

Tissue specificity

High expression in heart, liver, brain and pancreas; moderate in placenta, skeletal muscle and kidney; low in lung, prostate, testis, small intestine and colon. Isoform 1 expression is up-regulated in 64% hepatocellular carcinoma (HCC) patients. Ref.1

Sequence similarities

Belongs to the GAMAD family.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.
Isoform 1 (identifier: Q9NP97-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9NP97-2)

The sequence of this isoform differs from the canonical sequence as follows:
     28-47: IPIKSTMDNPTTTQYASLMH → GWEPLGHCGDRSRPPAQGCP
     48-96: Missing.
Note: May result from the retention of an intron in the cDNA.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.8
Chain2 – 9695Dynein light chain roadblock-type 1
PRO_0000220955

Amino acid modifications

Modified residue21N-acetylalanine By similarity

Natural variations

Alternative sequence28 – 4720IPIKS…ASLMH → GWEPLGHCGDRSRPPAQGCP in isoform 2.
VSP_007236
Alternative sequence48 – 9649Missing in isoform 2.
VSP_007237
Natural variant131S → R.
Corresponds to variant rs1063616 [ dbSNP | Ensembl ].
VAR_049124
Natural variant711I → F.
Corresponds to variant rs10036 [ dbSNP | Ensembl ].
VAR_049125

Secondary structure

................. 96
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: AAE1EFCD372897B3

FASTA9610,922
        10         20         30         40         50         60 
MAEVEETLKR LQSQKGVQGI IVVNTEGIPI KSTMDNPTTT QYASLMHSFI LKARSTVRDI 

        70         80         90 
DPQNDLTFLR IRSKKNEIMV APDKDYFLIV IQNPTE 

« Hide

Isoform 2 [UniParc].

Checksum: 17421DC72701A8A2
Show »

FASTA475,060

References

« Hide 'large scale' references
[1]"Identification of two novel human dynein light chain genes, DNLC2A and DNLC2B, and their expression changes in hepatocellular carcinoma tissues from 68 Chinese patients."
Jiang J., Yu L., Huang X., Chen X., Li D., Zhang Y., Tang L., Zhao S.
Gene 281:103-113(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
Tissue: Liver and Testis.
[2]"Novel genes expressed in hematopoietic stem/progenitor cells from myelodysplastic syndrome patients."
Gu J., Huang Q., Yu Y., Xu S., Han Z., Fu G., Zhou J., Wang Y., Huang C., Ren S., Tu Y., Chen Z.
Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Hematopoietic stem cell.
[3]"BitH, a human homolog of bithorax Drosophila melanogaster gene, on chromosome 20q."
Fracchiolla N.S., Cortelezzi A., Lambertenghi-Deliliers G.
Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[4]"Km23: role in growth factor signaling."
Tang Q., Staub C.M., Mulder K.M.
Submitted (JAN-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Placenta.
[5]"Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells."
Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G., Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W., Tao J., Huang Q.-H., Zhou J., Hu G.-X. expand/collapse author list , Gu J., Chen S.-J., Chen Z.
Genome Res. 10:1546-1560(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Umbilical cord blood.
[6]"The DNA sequence and comparative analysis of human chromosome 20."
Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. expand/collapse author list , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.
Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Kidney and Lymph.
[8]"Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides."
Gevaert K., Goethals M., Martens L., Van Damme J., Staes A., Thomas G.R., Vandekerckhove J.
Nat. Biotechnol. 21:566-569(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-10.
Tissue: Platelet.
[9]Lubec G., Chen W.-Q., Sun Y.
Submitted (DEC-2008) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 16-52 AND 59-70, IDENTIFICATION BY MASS SPECTROMETRY.
Tissue: Fetal brain cortex.
[10]"The roadblock light chain binds a novel region of the cytoplasmic Dynein intermediate chain."
Susalka S.J., Nikulina K., Salata M.W., Vaughan P.S., King S.M., Vaughan K.T., Pfister K.K.
J. Biol. Chem. 277:32939-32946(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH DYNC1I1 AND DYNC1I2.
[11]"Rab6 family proteins interact with the dynein light chain protein DYNLRB1."
Wanschers B.F.J., van de Vorstenbosch R., Wijers M., Wieringa B., King S.M., Fransen J.
Cell Motil. Cytoskeleton 65:183-196(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH RAP6A AND RAP6B.
[12]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[13]"Structure and dynamics of the homodimeric dynein light chain km23."
Ilangovan U., Ding W., Zhong Y., Wilson C.L., Groppe J.C., Trbovich J.T., Zuniga J., Demeler B., Tang Q., Gao G., Mulder K.M., Hinck A.P.
J. Mol. Biol. 352:338-354(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR, SUBUNIT.
[14]"Crystal structure of human dynein light chain Dnlc2A: structural insights into the interaction with IC74."
Liu J.F., Wang Z.X., Wang X.Q., Tang Q., An X.M., Gui L.L., Liang D.C.
Biochem. Biophys. Res. Commun. 349:1125-1129(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS), SUBUNIT.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF132750 mRNA. Translation: AAL75951.1.
AF165516 mRNA. Translation: AAF86646.1.
AF178431 mRNA. Translation: AAK95342.1.
AY026513 mRNA. Translation: AAK18712.1.
AF161511 mRNA. Translation: AAF29126.1.
AL109923, AL136173 Genomic DNA. Translation: CAI21456.1.
AL136173, AL109923 Genomic DNA. Translation: CAI23308.1.
AL109923, AL136173 Genomic DNA. Translation: CAM28250.1.
AL136173, AL109923 Genomic DNA. Translation: CAM28343.1.
BC002481 mRNA. Translation: AAH02481.1.
BC007223 mRNA. No translation available.
CCDSCCDS13235.1. [Q9NP97-1]
RefSeqNP_001268656.1. NM_001281727.1.
NP_001268657.1. NM_001281728.1.
NP_001268658.1. NM_001281729.1.
NP_054902.1. NM_014183.3. [Q9NP97-1]
NP_808852.1. NM_177953.2.
UniGeneHs.593920.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1Z09NMR-A/B1-96[»]
2B95NMR-A/B1-96[»]
2E8JNMR-A/B1-96[»]
2HZ5X-ray2.10A/B1-96[»]
ProteinModelPortalQ9NP97.
SMRQ9NP97. Positions 1-96.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid123715. 22 interactions.
IntActQ9NP97. 10 interactions.
MINTMINT-1485399.
STRING9606.ENSP00000349679.

PTM databases

PhosphoSiteQ9NP97.

Proteomic databases

MaxQBQ9NP97.
PaxDbQ9NP97.
PRIDEQ9NP97.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000300469; ENSP00000300469; ENSG00000125971. [Q9NP97-2]
ENST00000357156; ENSP00000349679; ENSG00000125971. [Q9NP97-1]
GeneID83658.
KEGGhsa:83658.
UCSCuc002xal.3. human. [Q9NP97-1]

Organism-specific databases

CTD83658.
GeneCardsGC20P033104.
HGNCHGNC:15468. DYNLRB1.
HPACAB009845.
MIM607167. gene.
neXtProtNX_Q9NP97.
PharmGKBPA27436.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG309619.
HOGENOMHOG000232033.
HOVERGENHBG058598.
KOK10419.
OMAQKEARQH.
OrthoDBEOG7X0VKJ.
PhylomeDBQ9NP97.
TreeFamTF315165.

Gene expression databases

ArrayExpressQ9NP97.
BgeeQ9NP97.
CleanExHS_DYNLRB1.
GenevestigatorQ9NP97.

Family and domain databases

InterProIPR004942. Dynein_light-rel.
IPR016561. Dynein_light_roadblock-type.
[Graphical view]
PfamPF03259. Robl_LC7. 1 hit.
[Graphical view]
PIRSFPIRSF009998. DLC7. 1 hit.
SMARTSM00960. Robl_LC7. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSDYNLRB1. human.
EvolutionaryTraceQ9NP97.
GeneWikiDYNLRB1.
GenomeRNAi83658.
NextBio72623.
PROQ9NP97.
SOURCESearch...

Entry information

Entry nameDLRB1_HUMAN
AccessionPrimary (citable) accession number: Q9NP97
Secondary accession number(s): B1AKR5 expand/collapse secondary AC list , Q5TC72, Q96IV3, Q9NQM2
Entry history
Integrated into UniProtKB/Swiss-Prot: April 23, 2003
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 127 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 20

Human chromosome 20: entries, gene names and cross-references to MIM