Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q9NP64 (NO40_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 112. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nucleolar protein of 40 kDa

Short name=pNO40
Alternative name(s):
Pnn-interacting nucleolar protein
Putative S1 RNA-binding domain protein
Short name=PS1D protein
Zinc finger CCHC domain-containing protein 17
Gene names
Name:ZCCHC17
Synonyms:PS1D
ORF Names:HSPC243, HSPC251, LDC4
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length241 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Subunit structure

Interacts with PNN. Associates with the 60S ribosomal subunit By similarity. Ref.1

Subcellular location

Nucleusnucleolus Ref.1.

Sequence similarities

Contains 1 CCHC-type zinc finger.

Contains 1 S1 motif domain.

Sequence caution

The sequence AAF36171.1 differs from that shown. Reason: Frameshift at position 48.

Ontologies

Keywords
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
   DomainZinc-finger
   LigandMetal-binding
Zinc
   Molecular functionRibonucleoprotein
   PTMAcetylation
Phosphoprotein
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentcytosolic large ribosomal subunit

Inferred from electronic annotation. Source: Ensembl

nucleolus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionpoly(A) RNA binding

Inferred from direct assay PubMed 22658674PubMed 22681889. Source: UniProtKB

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

JMJD6Q6NYC12EBI-746345,EBI-8464037

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9NP64-1)

Also known as: hpNO40;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9NP64-2)

Also known as: hpNO40s;

The sequence of this isoform differs from the canonical sequence as follows:
     1-24: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 241241Nucleolar protein of 40 kDa
PRO_0000096902

Regions

Domain16 – 8873S1 motif
Zinc finger131 – 14818CCHC-type
Compositional bias110 – 1134Poly-Arg
Compositional bias161 – 1677Poly-Glu
Compositional bias185 – 24056Lys-rich

Amino acid modifications

Modified residue1141Phosphoserine Ref.7 Ref.8 Ref.9
Modified residue1441N6-acetyllysine By similarity

Natural variations

Alternative sequence1 – 2424Missing in isoform 2.
VSP_015308

Experimental info

Sequence conflict2171R → G in AAF36171. Ref.2

Secondary structure

................ 241
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (hpNO40) [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: DA1B039DAC5F4A1D

FASTA24127,570
        10         20         30         40         50         60 
MNSGRPETME NLPALYTIFQ GEVAMVTDYG AFIKIPGCRK QGLVHRTHMS SCRVDKPSEI 

        70         80         90        100        110        120 
VDVGDKVWVK LIGREMKNDR IKVSLSMKVV NQGTGKDLDP NNVIIEQEER RRRSFQDYTG 

       130        140        150        160        170        180 
QKITLEAVLN TTCKKCGCKG HFAKDCFMQP GGTKYSLIPD EEEEKEEAKS AEFEKPDPTR 

       190        200        210        220        230        240 
NPSRKRKKEK KKKKHRDRKS SDSDSSDSES DTGKRARHTS KDSKAAKKKK KKKKHKKKHK 


E 

« Hide

Isoform 2 (hpNO40s) [UniParc].

Checksum: 9082B1330EE4DAAF
Show »

FASTA21724,919

References

« Hide 'large scale' references
[1]"Molecular characterization of a novel nucleolar protein, pNO40."
Chang W.-L., Lee D.-C., Leu S., Huang Y.-M., Lu M.-C., Ouyang P.
Biochem. Biophys. Res. Commun. 307:569-577(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), ALTERNATIVE SPLICING, SUBCELLULAR LOCATION, INTERACTION WITH PNN.
Tissue: Kidney.
[2]"Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells."
Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G., Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W., Tao J., Huang Q.-H., Zhou J., Hu G.-X. expand/collapse author list , Gu J., Chen S.-J., Chen Z.
Genome Res. 10:1546-1560(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Umbilical cord blood.
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Placenta, Retinoblastoma and Teratocarcinoma.
[4]"The DNA sequence and biological annotation of human chromosome 1."
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. expand/collapse author list , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Kidney, Muscle and Uterus.
[7]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-114, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[8]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-114, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[9]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-114, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"Solution structure of the S1 RNA binding domain of human hypothetical protein FLJ11067."
RIKEN structural genomics initiative (RSGI)
Submitted (NOV-2005) to the PDB data bank
Cited for: STRUCTURE BY NMR OF 1-106.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF247661 mRNA. Translation: AAF72518.3.
AF151077 mRNA. Translation: AAF36163.1.
AF151085 mRNA. Translation: AAF36171.1. Frameshift.
AK001929 mRNA. Translation: BAA91984.1.
AK022506 mRNA. Translation: BAB14066.1.
AK024049 mRNA. Translation: BAB14799.1.
AL451070 Genomic DNA. Translation: CAH71849.1.
CH471059 Genomic DNA. Translation: EAX07621.1.
CH471059 Genomic DNA. Translation: EAX07624.1.
BC000685 mRNA. Translation: AAH00685.1.
BC007446 mRNA. Translation: AAH07446.1.
BC050609 mRNA. Translation: AAH50609.1.
RefSeqNP_001269495.1. NM_001282566.1.
NP_001269496.1. NM_001282567.1.
NP_001269497.1. NM_001282568.1.
NP_001269498.1. NM_001282569.1.
NP_001269499.1. NM_001282570.1.
NP_001269500.1. NM_001282571.1.
NP_001269501.1. NM_001282572.1.
NP_001269502.1. NM_001282573.1.
NP_001269503.1. NM_001282574.1.
NP_057589.2. NM_016505.3.
UniGeneHs.524094.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2CQONMR-A1-106[»]
ProteinModelPortalQ9NP64.
SMRQ9NP64. Positions 5-106.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid119598. 9 interactions.
IntActQ9NP64. 6 interactions.
MINTMINT-1447770.
STRING9606.ENSP00000343557.

PTM databases

PhosphoSiteQ9NP64.

Polymorphism databases

DMDM73921227.

Proteomic databases

PaxDbQ9NP64.
PRIDEQ9NP64.

Protocols and materials databases

DNASU51538.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000344147; ENSP00000343557; ENSG00000121766. [Q9NP64-1]
ENST00000373714; ENSP00000362819; ENSG00000121766. [Q9NP64-1]
GeneID51538.
KEGGhsa:51538.
UCSCuc001bsp.1. human. [Q9NP64-1]

Organism-specific databases

CTD51538.
GeneCardsGC01P031769.
HGNCHGNC:30246. ZCCHC17.
HPAHPA028280.
neXtProtNX_Q9NP64.
PharmGKBPA142670539.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG1185.
HOGENOMHOG000113809.
HOVERGENHBG080343.
InParanoidQ9NP64.
OrthoDBEOG7NCV4Q.
PhylomeDBQ9NP64.
TreeFamTF332136.

Gene expression databases

ArrayExpressQ9NP64.
BgeeQ9NP64.
CleanExHS_ZCCHC17.
GenevestigatorQ9NP64.

Family and domain databases

Gene3D2.40.50.140. 1 hit.
InterProIPR012340. NA-bd_OB-fold.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR022967. RNA-binding_domain_S1.
IPR001878. Znf_CCHC.
[Graphical view]
PfamPF00575. S1. 1 hit.
[Graphical view]
SMARTSM00316. S1. 1 hit.
SM00343. ZnF_C2HC. 1 hit.
[Graphical view]
SUPFAMSSF50249. SSF50249. 1 hit.
PROSITEPS50126. S1. 1 hit.
PS50158. ZF_CCHC. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSZCCHC17. human.
EvolutionaryTraceQ9NP64.
GeneWikiZCCHC17.
GenomeRNAi51538.
NextBio55305.
PROQ9NP64.

Entry information

Entry nameNO40_HUMAN
AccessionPrimary (citable) accession number: Q9NP64
Secondary accession number(s): D3DPN4 expand/collapse secondary AC list , Q6PKH4, Q9NYG4, Q9P0M8
Entry history
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: October 1, 2000
Last modified: April 16, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM