Reviewed, UniProtKB/Swiss-Prot Q9NP61 (ARFG3_HUMAN)

Names and origin

Protein names

Recommended name:
    ADP-ribosylation factor GTPase-activating protein 3
      Short name=ARF GAP 3

Gene names

Name:	ARFGAP3
Synonyms:	ARFGAP1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	516 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	GTPase-activating protein (GAP) for ADP ribosylation factor 1 (ARF1). Hydrolysis of ARF1-bound GTP may lead to dissociation of coatomer from Golgi-derived membranes to allow fusion with target membranes. Ref.8
Enzyme regulation	GAP activity stimulated by phosphatidylinositol 4,5-bisphosphate (PIP2). Ref.8
Subcellular location	Cytoplasm. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side. Note: Also found on peripheral punctate structures likely to be endoplasmic reticulum-Golgi intermediate compartment. Ref.11
Tissue specificity	Widely expressed. Highest expression in endocrine glands (pancreas, pituary gland, salivary gland, and prostate) and testis with a much higher expression in the testis than in the ovary. Ref.1
Developmental stage	Expressed at higher level in adult thymus, brain and lung, than in corresponding fetal tissues. Expressed at lower level in spleen, heart, kidney and liver during development.
Sequence similarities	Contains 1 Arf-GAP domain.
Caution	Was originally (Ref.1) termed ARFGAP1.

Ontologies

Keywords
Biological process	ER-Golgi transport Protein transport Transport
Cellular component	Cytoplasm Golgi apparatus Membrane
Coding sequence diversity	Polymorphism
Domain	Coiled coil Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	GTPase activation
PTM	Phosphoprotein
Technical term	3D-structure
Gene Ontology (GO)
Biological process	intracellular protein transport Ref.8 Non-traceable author statement. Source: UniProtKB protein secretion Ref.8 Inferred from expression pattern. Source: UniProtKB regulation of ARF GTPase activity Inferred from electronic annotation. Source: InterPro vesicle-mediated transport Ref.8 Non-traceable author statement. Source: UniProtKB
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell cytosol Ref.8 Inferred from direct assay. Source: UniProtKB nucleus Inferred from direct assay. Source: HPA
Molecular function	ARF GTPase activator activity Ref.8 Inferred from direct assay. Source: UniProtKB protein transporter activity Ref.8 Non-traceable author statement. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 516

516

ADP-ribosylation factor GTPase-activating protein 3

PRO_0000074193

Regions

Domain

10 – 126

117

Arf-GAP

Zinc finger

25 – 48

24

C4-type

Coiled coil

243 – 263

21

Potential

Compositional bias

348 – 377

30

Ser-rich

Amino acid modifications

Modified residue

143

1

Phosphoserine Ref.9

Modified residue

270

1

Phosphoserine Ref.12

Modified residue

331

1

Phosphoserine Ref.10 Ref.13

Modified residue

453

1

Phosphoserine Ref.13

Natural variations

Natural variant

231

1

S → G: dbSNP rs9607957.

VAR_055523

Natural variant

290

1

E → G in a breast cancer sample; somatic mutation. Ref.16

VAR_036177

Natural variant

355

1

S → R: dbSNP rs1018448. Ref.3 Ref.5 Ref.7

VAR_013447

Natural variant

370

1

S → G: dbSNP rs16986123.

VAR_055524

Natural variant

468

1

Q → H: dbSNP rs35498349.

VAR_055525

Natural variant

482

1

A → T: dbSNP rs36003980.

VAR_055526

Natural variant

490

1

Q → R: dbSNP rs11551619.

VAR_055527

Experimental info

Sequence conflict

339

1

K → R in BAA92076. Ref.7

Sequence conflict

401

1

T → A in BAA92076. Ref.7

Sequence conflict

458

1

S → P in BAB14236. Ref.7

Secondary structure

1

.

.

.

.

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.

.

.

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.

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.

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.

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.

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516

Helix Strand Turn

Details...

Helix

6 – 18

13

Turn

20 – 22

3

Beta strand

26 – 28

3

Turn

38 – 41

4

Helix

46 – 55

10

Turn

57 – 59

3

Beta strand

65 – 67

3

Helix

73 – 80

8

Helix

84 – 94

11

Helix

101 – 104

4

Helix

108 – 126

19

Beta strand

129 – 131

3

Sequences

	Sequence		Length	Mass (Da)
	Q9NP61-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: E355E56A5D867F8E	FASTA	516	56,928
	10 20 30 40 50 60 MGDPSKQDIL TIFKRLRSVP TNKVCFDCGA KNPSWASITY GVFLCIDCSG SHRSLGVHLS 70 80 90 100 110 120 FIRSTELDSN WSWFQLRCMQ VGGNASASSF FHQHGCSTND TNAKYNSRAA QLYREKIKSL 130 140 150 160 170 180 ASQATRKHGT DLWLDSCVVP PLSPPPKEED FFASHVSPEV SDTAWASAIA EPSSLTSRPV 190 200 210 220 230 240 ETTLENNEGG QEQGPSVEGL NVPTKATLEV SSIIKKKPNQ AKKGLGAKKG SLGAQKLANT 250 260 270 280 290 300 CFNEIEKQAQ AADKMKEQED LAKVVSKEES IVSSLRLAYK DLEIQMKKDE KMNISGKKNV 310 320 330 340 350 360 DSDRLGMGFG NCRSVISHSV TSDMQTIEQE SPIMAKPRKK YNDDSDDSYF TSSSSYFDEP 370 380 390 400 410 420 VELRSSSFSS WDDSSDSYWK KETSKDTETV LKTTGYSDRP TARRKPDYEP VENTDEAQKK 430 440 450 460 470 480 FGNVKAISSD MYFGRQSQAD YETRARLERL SASSSISSAD LFEEPRKQPA GNYSLSSVLP 490 500 510 NAPDMAQFKQ GVRSVAGKLS VFANGVVTSI QDRYGS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization, chromosomal assignment, and tissue expression of a novel human gene belonging to the ARF GAP family." Zhang C., Yu Y., Zhang S., Liu M., Xing G., Wei H., Bi J., Liu X., Zhou G., Dong C., Hu Z., Zhang Y., Luo L., Wu C., Zhao S., He F. Genomics 63:400-408(2000) [PubMed: 10704287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Tissue: Placenta.
[2]	"Reevaluating human gene annotation: a second-generation analysis of chromosome 22." Collins J.E., Goward M.E., Cole C.G., Smink L.J., Huckle E.J., Knowles S., Bye J.M., Beare D.M., Dunham I. Genome Res. 13:27-36(2003) [PubMed: 12529303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"A genome annotation-driven approach to cloning the human ORFeome." Collins J.E., Wright C.L., Edwards C.A., Davis M.P., Grinham J.A., Cole C.G., Goward M.E., Aguado B., Mallya M., Mokrab Y., Huckle E.J., Beare D.M., Dunham I. Genome Biol. 5:RESEARCH84.1-RESEARCH84.11(2004) [PubMed: 15461802] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ARG-355.
[4]	"The DNA sequence of human chromosome 22." Dunham I., Hunt A.R., Collins J.E., Bruskiewich R., Beare D.M., Clamp M., Smink L.J., Ainscough R., Almeida J.P., Babbage A.K., Bagguley C., Bailey J., Barlow K.F., Bates K.N., Beasley O.P., Bird C.P., Blakey S.E., Bridgeman A.M. , Buck D., Burgess J., Burrill W.D., Burton J., Carder C., Carter N.P., Chen Y., Clark G., Clegg S.M., Cobley V.E., Cole C.G., Collier R.E., Connor R., Conroy D., Corby N.R., Coville G.J., Cox A.V., Davis J., Dawson E., Dhami P.D., Dockree C., Dodsworth S.J., Durbin R.M., Ellington A.G., Evans K.L., Fey J.M., Fleming K., French L., Garner A.A., Gilbert J.G.R., Goward M.E., Grafham D.V., Griffiths M.N.D., Hall C., Hall R.E., Hall-Tamlyn G., Heathcott R.W., Ho S., Holmes S., Hunt S.E., Jones M.C., Kershaw J., Kimberley A.M., King A., Laird G.K., Langford C.F., Leversha M.A., Lloyd C., Lloyd D.M., Martyn I.D., Mashreghi-Mohammadi M., Matthews L.H., Mccann O.T., Mcclay J., Mclaren S., McMurray A.A., Milne S.A., Mortimore B.J., Odell C.N., Pavitt R., Pearce A.V., Pearson D., Phillimore B.J.C.T., Phillips S.H., Plumb R.W., Ramsay H., Ramsey Y., Rogers L., Ross M.T., Scott C.E., Sehra H.K., Skuce C.D., Smalley S., Smith M.L., Soderlund C., Spragon L., Steward C.A., Sulston J.E., Swann R.M., Vaudin M., Wall M., Wallis J.M., Whiteley M.N., Willey D.L., Williams L., Williams S.A., Williamson H., Wilmer T.E., Wilming L., Wright C.L., Hubbard T., Bentley D.R., Beck S., Rogers J., Shimizu N., Minoshima S., Kawasaki K., Sasaki T., Asakawa S., Kudoh J., Shintani A., Shibuya K., Yoshizaki Y., Aoki N., Mitsuyama S., Roe B.A., Chen F., Chu L., Crabtree J., Deschamps S., Do A., Do T., Dorman A., Fang F., Fu Y., Hu P., Hua A., Kenton S., Lai H., Lao H.I., Lewis J., Lewis S., Lin S.-P., Loh P., Malaj E., Nguyen T., Pan H., Phan S., Qi S., Qian Y., Ray L., Ren Q., Shaull S., Sloan D., Song L., Wang Q., Wang Y., Wang Z., White J., Willingham D., Wu H., Yao Z., Zhan M., Zhang G., Chissoe S., Murray J., Miller N., Minx P., Fulton R., Johnson D., Bemis G., Bentley D., Bradshaw H., Bourne S., Cordes M., Du Z., Fulton L., Goela D., Graves T., Hawkins J., Hinds K., Kemp K., Latreille P., Layman D., Ozersky P., Rohlfing T., Scheet P., Walker C., Wamsley A., Wohldmann P., Pepin K., Nelson J., Korf I., Bedell J.A., Hillier L.W., Mardis E., Waterston R., Wilson R., Emanuel B.S., Shaikh T., Kurahashi H., Saitta S., Budarf M.L., McDermid H.E., Johnson A., Wong A.C.C., Morrow B.E., Edelmann L., Kim U.J., Shizuya H., Simon M.I., Dumanski J.P., Peyrard M., Kedra D., Seroussi E., Fransson I., Tapia I., Bruder C.E., O'Brien K.P., Wilkinson P., Bodenteich A., Hartman K., Hu X., Khan A.S., Lane L., Tilahun Y., Wright H. Nature 402:489-495(1999) [PubMed: 10591208] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ARG-355. Tissue: Lung.
[6]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Blocker H., Heubner D., Hoerlein A., Michel G., Wedler H., Kohrer K., Ottenwalder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 44-516. Tissue: Testis.
[7]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 118-516, VARIANT ARG-355. Tissue: Placenta.
[8]	"Functional characterization of novel human ARFGAP3." Liu X., Zhang C., Xing G., Chen Q., He F. FEBS Lett. 490:79-83(2001) [PubMed: 11172815] [Abstract] Cited for: FUNCTION, ENZYME REGULATION.
[9]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-143, MASS SPECTROMETRY. Tissue: Epithelium.
[10]	"Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra." Yu L.-R., Zhu Z., Chan K.C., Issaq H.J., Dimitrov D.S., Veenstra T.D. J. Proteome Res. 6:4150-4162(2007) [PubMed: 17924679] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-331, MASS SPECTROMETRY. Tissue: Epithelium.
[11]	"Two human ARFGAPs associated with COP-I-coated vesicles." Frigerio G., Grimsey N., Dale M., Majoul I., Duden R. Traffic 8:1644-1655(2007) [PubMed: 17760859] [Abstract] Cited for: SUBCELLULAR LOCATION.
[12]	"Automated phosphoproteome analysis for cultured cancer cells by two-dimensional nanoLC-MS using a calcined titania/C18 biphasic column." Imami K., Sugiyama N., Kyono Y., Tomita M., Ishihama Y. Anal. Sci. 24:161-166(2008) [PubMed: 18187866] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-270, MASS SPECTROMETRY.
[13]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-331 AND SER-453, MASS SPECTROMETRY.
[14]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[15]	"Solution structure of the ARFGAP domain of ADP-ribosylation factor GTPase-activating protein 3 (ARFGAP 3)." RIKEN structural genomics initiative (RSGI) Submitted (NOV-2005) to the PDB data bank Cited for: STRUCTURE BY NMR OF 1-136.
[16]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed: 16959974] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] GLY-290.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AF111847 mRNA. Translation: AAF40310.1.
AL159143 mRNA. Translation: CAB76901.1.
CR456382 mRNA. Translation: CAG30268.1.
AL049757, AL049758 Genomic DNA. Translation: CAI21510.1.
AL049758, AL049757 Genomic DNA. Translation: CAI20950.1.
BC005122 mRNA. Translation: AAH05122.1.
AL137598 mRNA. Translation: CAB70834.1.
AK002083 mRNA. Translation: BAA92076.1. Different initiation.
AK022768 mRNA. Translation: BAB14236.1. Different initiation.

IPI

IPI00299263.

PIR

T46305.

RefSeq

NP_001135765.1.
NP_055385.3.

UniGene

Hs.685225

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2CRW	NMR	-	A	1-136	[»]

ModBase

Search...

PTM databases

PhosphoSite

Q9NP61.

Proteomic databases

PeptideAtlas

Q9NP61.

PRIDE

Q9NP61.

Genome annotation databases

Ensembl

ENSG00000100262. Homo sapiens. [Contig view]

GeneID

26286.

KEGG

hsa:26286.

Organism-specific databases

GeneCards

GC22M041517.

H-InvDB

HIX0009609.
HIX0016547.

HGNC

HGNC:661. ARFGAP3.

HPA

HPA000638.

MIM

612439. gene.

PharmGKB

PA35024.

GenAtlas

Search...

Phylogenomic databases

HOVERGEN

Q9NP61.

OMA

Q9NP61. KPDYEPV.

Gene expression databases

ArrayExpress

Q9NP61.

Bgee

Q9NP61.

CleanEx

HS_ARFGAP1.
HS_ARFGAP3.

GermOnline

ENSG00000100262. Homo sapiens.

Family and domain databases

InterPro

IPR001164. ArfGAP.
[Graphical view]

Pfam

PF01412. ArfGap. 1 hit.
[Graphical view]

PRINTS

PR00405. REVINTRACTNG.

SMART

SM00105. ArfGap. 1 hit.
[Graphical view]

PROSITE

PS50115. ARFGAP. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

48617.

SOURCE

Search...

Entry information

Entry name

ARFG3_HUMAN

Accession

Primary (citable) accession number: Q9NP61
Secondary accession number(s): Q9BSC6 , Q9H9J0, Q9NT10, Q9NUP5, Q9Y4V3, Q9Y4V4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 27, 2002
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)