Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

ADP-ribosylation factor GTPase-activating protein 3

Gene

ARFGAP3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GTPase-activating protein (GAP) for ADP ribosylation factor 1 (ARF1). Hydrolysis of ARF1-bound GTP may lead to dissociation of coatomer from Golgi-derived membranes to allow fusion with target membranes.1 Publication

Enzyme regulationi

GAP activity stimulated by phosphatidylinositol 4,5-bisphosphate (PIP2).1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri25 – 48C4-typePROSITE-ProRule annotationAdd BLAST24

GO - Molecular functioni

  • GTPase activator activity Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW
  • protein transporter activity Source: UniProtKB

GO - Biological processi

  • ER to Golgi vesicle-mediated transport Source: Reactome
  • intracellular protein transport Source: UniProtKB
  • protein secretion Source: UniProtKB
  • retrograde vesicle-mediated transport, Golgi to ER Source: Reactome
  • vesicle-mediated transport Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-6807878. COPI-mediated anterograde transport.
R-HSA-6811434. COPI-dependent Golgi-to-ER retrograde traffic.

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribosylation factor GTPase-activating protein 3
Short name:
ARF GAP 3
Gene namesi
Name:ARFGAP3
Synonyms:ARFGAP1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 22

Organism-specific databases

HGNCiHGNC:661. ARFGAP3.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: HPA
  • cytosol Source: UniProtKB
  • Golgi apparatus Source: HPA
  • Golgi membrane Source: UniProtKB-SubCell
  • membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi26286.
OpenTargetsiENSG00000242247.
PharmGKBiPA35024.

Polymorphism and mutation databases

BioMutaiARFGAP3.
DMDMi21263420.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000741931 – 516ADP-ribosylation factor GTPase-activating protein 3Add BLAST516

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei231PhosphoserineCombined sources1
Modified residuei270PhosphoserineCombined sources1
Modified residuei274PhosphoserineCombined sources1
Modified residuei331PhosphoserineCombined sources1
Modified residuei370PhosphoserineCombined sources1
Modified residuei428PhosphoserineCombined sources1
Modified residuei451PhosphoserineCombined sources1
Modified residuei453PhosphoserineCombined sources1
Modified residuei455PhosphoserineCombined sources1
Modified residuei457PhosphoserineCombined sources1
Modified residuei458PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9NP61.
MaxQBiQ9NP61.
PaxDbiQ9NP61.
PeptideAtlasiQ9NP61.
PRIDEiQ9NP61.
TopDownProteomicsiQ9NP61-1. [Q9NP61-1]

PTM databases

iPTMnetiQ9NP61.
PhosphoSitePlusiQ9NP61.

Expressioni

Tissue specificityi

Widely expressed. Highest expression in endocrine glands (pancreas, pituitary gland, salivary gland, and prostate) and testis with a much higher expression in the testis than in the ovary.1 Publication

Developmental stagei

Expressed at higher level in adult thymus, brain and lung, than in corresponding fetal tissues. Expressed at lower level in spleen, heart, kidney and liver during development.

Gene expression databases

BgeeiENSG00000242247.
CleanExiHS_ARFGAP1.
HS_ARFGAP3.
ExpressionAtlasiQ9NP61. baseline and differential.
GenevisibleiQ9NP61. HS.

Organism-specific databases

HPAiHPA000638.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
ZDHHC17Q8IUH52EBI-2875816,EBI-524753

Protein-protein interaction databases

BioGridi117668. 19 interactors.
IntActiQ9NP61. 14 interactors.
STRINGi9606.ENSP00000263245.

Structurei

Secondary structure

1516
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 18Combined sources13
Turni20 – 22Combined sources3
Beta strandi26 – 28Combined sources3
Turni38 – 41Combined sources4
Helixi46 – 55Combined sources10
Turni57 – 59Combined sources3
Beta strandi65 – 67Combined sources3
Helixi73 – 80Combined sources8
Helixi84 – 94Combined sources11
Helixi101 – 104Combined sources4
Helixi108 – 126Combined sources19
Beta strandi129 – 131Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CRWNMR-A1-136[»]
ProteinModelPortaliQ9NP61.
SMRiQ9NP61.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9NP61.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini10 – 126Arf-GAPPROSITE-ProRule annotationAdd BLAST117

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili243 – 263Sequence analysisAdd BLAST21

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi348 – 377Ser-richAdd BLAST30

Sequence similaritiesi

Contains 1 Arf-GAP domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri25 – 48C4-typePROSITE-ProRule annotationAdd BLAST24

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiKOG0706. Eukaryota.
COG5347. LUCA.
GeneTreeiENSGT00390000005436.
HOVERGENiHBG050563.
InParanoidiQ9NP61.
KOiK12493.
OMAiWDDSADS.
OrthoDBiEOG091G0AS9.
PhylomeDBiQ9NP61.
TreeFamiTF313985.

Family and domain databases

InterProiIPR001164. ArfGAP.
[Graphical view]
PfamiPF01412. ArfGap. 1 hit.
[Graphical view]
PRINTSiPR00405. REVINTRACTNG.
SMARTiSM00105. ArfGap. 1 hit.
[Graphical view]
PROSITEiPS50115. ARFGAP. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9NP61-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGDPSKQDIL TIFKRLRSVP TNKVCFDCGA KNPSWASITY GVFLCIDCSG
60 70 80 90 100
SHRSLGVHLS FIRSTELDSN WSWFQLRCMQ VGGNASASSF FHQHGCSTND
110 120 130 140 150
TNAKYNSRAA QLYREKIKSL ASQATRKHGT DLWLDSCVVP PLSPPPKEED
160 170 180 190 200
FFASHVSPEV SDTAWASAIA EPSSLTSRPV ETTLENNEGG QEQGPSVEGL
210 220 230 240 250
NVPTKATLEV SSIIKKKPNQ AKKGLGAKKG SLGAQKLANT CFNEIEKQAQ
260 270 280 290 300
AADKMKEQED LAKVVSKEES IVSSLRLAYK DLEIQMKKDE KMNISGKKNV
310 320 330 340 350
DSDRLGMGFG NCRSVISHSV TSDMQTIEQE SPIMAKPRKK YNDDSDDSYF
360 370 380 390 400
TSSSSYFDEP VELRSSSFSS WDDSSDSYWK KETSKDTETV LKTTGYSDRP
410 420 430 440 450
TARRKPDYEP VENTDEAQKK FGNVKAISSD MYFGRQSQAD YETRARLERL
460 470 480 490 500
SASSSISSAD LFEEPRKQPA GNYSLSSVLP NAPDMAQFKQ GVRSVAGKLS
510
VFANGVVTSI QDRYGS
Length:516
Mass (Da):56,928
Last modified:October 1, 2000 - v1
Checksum:iE355E56A5D867F8E
GO
Isoform 2 (identifier: Q9NP61-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     88-131: Missing.

Note: Gene prediction based on EST data.
Show »
Length:472
Mass (Da):51,990
Checksum:i6F3600081545890B
GO

Sequence cautioni

The sequence BAA92076 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAB14236 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti339K → R in BAA92076 (PubMed:14702039).Curated1
Sequence conflicti401T → A in BAA92076 (PubMed:14702039).Curated1
Sequence conflicti458S → P in BAB14236 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_055523231S → G.Corresponds to variant rs9607957dbSNPEnsembl.1
Natural variantiVAR_036177290E → G in a breast cancer sample; somatic mutation. 1 Publication1
Natural variantiVAR_013447355S → R.Combined sources3 PublicationsCorresponds to variant rs1018448dbSNPEnsembl.1
Natural variantiVAR_055524370S → G.Corresponds to variant rs16986123dbSNPEnsembl.1
Natural variantiVAR_055525468Q → H.Corresponds to variant rs35498349dbSNPEnsembl.1
Natural variantiVAR_055526482A → T.Corresponds to variant rs36003980dbSNPEnsembl.1
Natural variantiVAR_055527490Q → R.Corresponds to variant rs11551619dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_04688888 – 131Missing in isoform 2. CuratedAdd BLAST44

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF111847 mRNA. Translation: AAF40310.1.
AL159143 mRNA. Translation: CAB76901.1.
CR456382 mRNA. Translation: CAG30268.1.
AL049757, AL049758 Genomic DNA. Translation: CAI21510.1.
AL049758, AL049757 Genomic DNA. Translation: CAI20950.1.
BC005122 mRNA. Translation: AAH05122.1.
AL137598 mRNA. Translation: CAB70834.1.
AK002083 mRNA. Translation: BAA92076.1. Different initiation.
AK022768 mRNA. Translation: BAB14236.1. Different initiation.
CCDSiCCDS14042.1. [Q9NP61-1]
CCDS46722.1. [Q9NP61-2]
PIRiT46305.
RefSeqiNP_001135765.1. NM_001142293.1. [Q9NP61-2]
NP_055385.3. NM_014570.4. [Q9NP61-1]
UniGeneiHs.685225.

Genome annotation databases

EnsembliENST00000263245; ENSP00000263245; ENSG00000242247. [Q9NP61-1]
ENST00000437119; ENSP00000388791; ENSG00000242247. [Q9NP61-2]
GeneIDi26286.
KEGGihsa:26286.
UCSCiuc003bdd.3. human. [Q9NP61-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF111847 mRNA. Translation: AAF40310.1.
AL159143 mRNA. Translation: CAB76901.1.
CR456382 mRNA. Translation: CAG30268.1.
AL049757, AL049758 Genomic DNA. Translation: CAI21510.1.
AL049758, AL049757 Genomic DNA. Translation: CAI20950.1.
BC005122 mRNA. Translation: AAH05122.1.
AL137598 mRNA. Translation: CAB70834.1.
AK002083 mRNA. Translation: BAA92076.1. Different initiation.
AK022768 mRNA. Translation: BAB14236.1. Different initiation.
CCDSiCCDS14042.1. [Q9NP61-1]
CCDS46722.1. [Q9NP61-2]
PIRiT46305.
RefSeqiNP_001135765.1. NM_001142293.1. [Q9NP61-2]
NP_055385.3. NM_014570.4. [Q9NP61-1]
UniGeneiHs.685225.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CRWNMR-A1-136[»]
ProteinModelPortaliQ9NP61.
SMRiQ9NP61.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117668. 19 interactors.
IntActiQ9NP61. 14 interactors.
STRINGi9606.ENSP00000263245.

PTM databases

iPTMnetiQ9NP61.
PhosphoSitePlusiQ9NP61.

Polymorphism and mutation databases

BioMutaiARFGAP3.
DMDMi21263420.

Proteomic databases

EPDiQ9NP61.
MaxQBiQ9NP61.
PaxDbiQ9NP61.
PeptideAtlasiQ9NP61.
PRIDEiQ9NP61.
TopDownProteomicsiQ9NP61-1. [Q9NP61-1]

Protocols and materials databases

DNASUi26286.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000263245; ENSP00000263245; ENSG00000242247. [Q9NP61-1]
ENST00000437119; ENSP00000388791; ENSG00000242247. [Q9NP61-2]
GeneIDi26286.
KEGGihsa:26286.
UCSCiuc003bdd.3. human. [Q9NP61-1]

Organism-specific databases

CTDi26286.
DisGeNETi26286.
GeneCardsiARFGAP3.
HGNCiHGNC:661. ARFGAP3.
HPAiHPA000638.
MIMi612439. gene.
neXtProtiNX_Q9NP61.
OpenTargetsiENSG00000242247.
PharmGKBiPA35024.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0706. Eukaryota.
COG5347. LUCA.
GeneTreeiENSGT00390000005436.
HOVERGENiHBG050563.
InParanoidiQ9NP61.
KOiK12493.
OMAiWDDSADS.
OrthoDBiEOG091G0AS9.
PhylomeDBiQ9NP61.
TreeFamiTF313985.

Enzyme and pathway databases

ReactomeiR-HSA-6807878. COPI-mediated anterograde transport.
R-HSA-6811434. COPI-dependent Golgi-to-ER retrograde traffic.

Miscellaneous databases

ChiTaRSiARFGAP3. human.
EvolutionaryTraceiQ9NP61.
GeneWikiiARFGAP3.
GenomeRNAii26286.
PROiQ9NP61.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000242247.
CleanExiHS_ARFGAP1.
HS_ARFGAP3.
ExpressionAtlasiQ9NP61. baseline and differential.
GenevisibleiQ9NP61. HS.

Family and domain databases

InterProiIPR001164. ArfGAP.
[Graphical view]
PfamiPF01412. ArfGap. 1 hit.
[Graphical view]
PRINTSiPR00405. REVINTRACTNG.
SMARTiSM00105. ArfGap. 1 hit.
[Graphical view]
PROSITEiPS50115. ARFGAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiARFG3_HUMAN
AccessioniPrimary (citable) accession number: Q9NP61
Secondary accession number(s): E9PB03
, Q9BSC6, Q9H9J0, Q9NT10, Q9NUP5, Q9Y4V3, Q9Y4V4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 27, 2002
Last sequence update: October 1, 2000
Last modified: November 30, 2016
This is version 158 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

Was originally termed ARFGAP1.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 22
    Human chromosome 22: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.