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Q9NP56 (PDE7B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 124. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
cAMP-specific 3',5'-cyclic phosphodiesterase 7B

EC=3.1.4.53
Gene names
Name:PDE7B
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length450 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in the control of cAMP-mediated neural activity and cAMP metabolism in the brain.

Catalytic activity

Adenosine 3',5'-cyclic phosphate + H2O = adenosine 5'-phosphate.

Cofactor

Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.

Enzyme regulation

Inhibited by dipyridamole, IBMX and SCH 51866. Insensitive to zaprinast, rolipram, and milrinone.

Pathway

Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.

Tissue specificity

Highly expressed in brain. Also expressed in heart, liver, skeletal muscle and pancreas.

Domain

Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain.

Sequence similarities

Belongs to the cyclic nucleotide phosphodiesterase family. PDE7 subfamily.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 450450cAMP-specific 3',5'-cyclic phosphodiesterase 7B
PRO_0000198836

Regions

Region172 – 410239Catalytic By similarity

Sites

Active site1731Proton donor By similarity
Metal binding1771Divalent metal cation 1 By similarity
Metal binding2131Divalent metal cation 1 By similarity
Metal binding2141Divalent metal cation 1 By similarity
Metal binding2141Divalent metal cation 2 By similarity
Metal binding3231Divalent metal cation 1 By similarity

Secondary structure

...................................................... 450
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q9NP56 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: EC142BF3E28D0028

FASTA45051,835
        10         20         30         40         50         60 
MSCLMVERCG EILFENPDQN AKCVCMLGDI RLRGQTGVRA ERRGSYPFID FRLLNSTTYS 

        70         80         90        100        110        120 
GEIGTKKKVK RLLSFQRYFH ASRLLRGIIP QAPLHLLDED YLGQARHMLS KVGMWDFDIF 

       130        140        150        160        170        180 
LFDRLTNGNS LVTLLCHLFN THGLIHHFKL DMVTLHRFLV MVQEDYHSQN PYHNAVHAAD 

       190        200        210        220        230        240 
VTQAMHCYLK EPKLASFLTP LDIMLGLLAA AAHDVDHPGV NQPFLIKTNH HLANLYQNMS 

       250        260        270        280        290        300 
VLENHHWRST IGMLRESRLL AHLPKEMTQD IEQQLGSLIL ATDINRQNEF LTRLKAHLHN 

       310        320        330        340        350        360 
KDLRLEDAQD RHFMLQIALK CADICNPCRI WEMSKQWSER VCEEFYRQGE LEQKFELEIS 

       370        380        390        400        410        420 
PLCNQQKDSI PSIQIGFMSY IVEPLFREWA HFTGNSTLSE NMLGHLAHNK AQWKSLLPRQ 

       430        440        450 
HRSRGSSGSG PDHDHAGQGT ESEEQEGDSP 

« Hide

References

« Hide 'large scale' references
[1]"Identification of human PDE7B, a cAMP-specific phosphodiesterase."
Sasaki T., Kotera J., Yuasa K., Omori K.
Biochem. Biophys. Res. Commun. 271:575-583(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[2]"Cloning and characterisation of the human and mouse PDE7B, a novel cAMP-specific nucleotide phosphodiesterase."
Gardner C.E., Robas N.M., Cawkill D., Fidock M.D.
Biochem. Biophys. Res. Commun. 272:186-192(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Fetal brain.
[3]"The DNA sequence and analysis of human chromosome 6."
Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. expand/collapse author list , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[6]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB038040 mRNA. Translation: BAA96537.1.
AJ251860 mRNA. Translation: CAB92441.1.
AL360178, AL133319, AL138828 Genomic DNA. Translation: CAH73075.1.
AL133319, AL138828, AL360178 Genomic DNA. Translation: CAH73332.1.
AL138828, AL133319, AL360178 Genomic DNA. Translation: CAI95287.1.
CH471051 Genomic DNA. Translation: EAW47957.1.
BC075082 mRNA. Translation: AAH75082.1.
BC075083 mRNA. Translation: AAH75083.1.
CCDSCCDS5175.1.
PIRJC7266.
RefSeqNP_061818.1. NM_018945.3.
UniGeneHs.744230.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1LXWmodel-A104-433[»]
ProteinModelPortalQ9NP56.
SMRQ9NP56. Positions 100-416.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid118010. 2 interactions.
IntActQ9NP56. 1 interaction.
STRING9606.ENSP00000310661.

Chemistry

BindingDBQ9NP56.
ChEMBLCHEMBL2111411.
DrugBankDB00651. Dyphylline.
DB00920. Ketotifen.
GuidetoPHARMACOLOGY1306.

PTM databases

PhosphoSiteQ9NP56.

Polymorphism databases

DMDM13626185.

Proteomic databases

PaxDbQ9NP56.
PRIDEQ9NP56.

Protocols and materials databases

DNASU27115.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000308191; ENSP00000310661; ENSG00000171408.
GeneID27115.
KEGGhsa:27115.
UCSCuc003qgp.3. human.

Organism-specific databases

CTD27115.
GeneCardsGC06P136214.
HGNCHGNC:8792. PDE7B.
HPAHPA023967.
MIM604645. gene.
neXtProtNX_Q9NP56.
PharmGKBPA33140.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG300643.
HOGENOMHOG000220881.
HOVERGENHBG053543.
KOK01120.
OMANNTTHSG.
OrthoDBEOG7M98G3.
PhylomeDBQ9NP56.
TreeFamTF314638.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.
UniPathwayUPA00762; UER00747.

Gene expression databases

BgeeQ9NP56.
CleanExHS_PDE7B.
GenevestigatorQ9NP56.

Family and domain databases

Gene3D1.10.1300.10. 1 hit.
InterProIPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view]
PfamPF00233. PDEase_I. 1 hit.
[Graphical view]
PRINTSPR00387. PDIESTERASE1.
SMARTSM00471. HDc. 1 hit.
[Graphical view]
PROSITEPS00126. PDEASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi27115.
NextBio49800.
PROQ9NP56.
SOURCESearch...

Entry information

Entry namePDE7B_HUMAN
AccessionPrimary (citable) accession number: Q9NP56
Secondary accession number(s): Q5W154
Entry history
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: October 1, 2000
Last modified: July 9, 2014
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 6

Human chromosome 6: entries, gene names and cross-references to MIM