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Q9NP56

- PDE7B_HUMAN

UniProt

Q9NP56 - PDE7B_HUMAN

Protein

cAMP-specific 3',5'-cyclic phosphodiesterase 7B

Gene

PDE7B

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 126 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in the control of cAMP-mediated neural activity and cAMP metabolism in the brain.

    Catalytic activityi

    Adenosine 3',5'-cyclic phosphate + H2O = adenosine 5'-phosphate.

    Cofactori

    Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.By similarity

    Enzyme regulationi

    Inhibited by dipyridamole, IBMX and SCH 51866. Insensitive to zaprinast, rolipram, and milrinone.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei173 – 1731Proton donorBy similarity
    Metal bindingi177 – 1771Divalent metal cation 1By similarity
    Metal bindingi213 – 2131Divalent metal cation 1By similarity
    Metal bindingi214 – 2141Divalent metal cation 1By similarity
    Metal bindingi214 – 2141Divalent metal cation 2By similarity
    Metal bindingi323 – 3231Divalent metal cation 1By similarity

    GO - Molecular functioni

    1. 3',5'-cyclic-AMP phosphodiesterase activity Source: ProtInc
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. cAMP catabolic process Source: UniProtKB-UniPathway
    2. signal transduction Source: ProtInc
    3. synaptic transmission Source: ProtInc

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    cAMP, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_19327. G alpha (s) signalling events.
    UniPathwayiUPA00762; UER00747.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    cAMP-specific 3',5'-cyclic phosphodiesterase 7B (EC:3.1.4.53)
    Gene namesi
    Name:PDE7B
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 6

    Organism-specific databases

    HGNCiHGNC:8792. PDE7B.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: Reactome

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA33140.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 450450cAMP-specific 3',5'-cyclic phosphodiesterase 7BPRO_0000198836Add
    BLAST

    Proteomic databases

    PaxDbiQ9NP56.
    PRIDEiQ9NP56.

    PTM databases

    PhosphoSiteiQ9NP56.

    Expressioni

    Tissue specificityi

    Highly expressed in brain. Also expressed in heart, liver, skeletal muscle and pancreas.

    Gene expression databases

    BgeeiQ9NP56.
    CleanExiHS_PDE7B.
    GenevestigatoriQ9NP56.

    Organism-specific databases

    HPAiHPA023967.

    Interactioni

    Protein-protein interaction databases

    BioGridi118010. 2 interactions.
    IntActiQ9NP56. 1 interaction.
    STRINGi9606.ENSP00000310661.

    Structurei

    Secondary structure

    1
    450
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi105 – 1084
    Helixi109 – 1113
    Helixi119 – 1257
    Turni126 – 1283
    Helixi130 – 14112
    Helixi144 – 1474
    Helixi152 – 16413
    Beta strandi170 – 1745
    Helixi175 – 18915
    Helixi192 – 1943
    Turni195 – 1973
    Helixi200 – 21213
    Turni213 – 2164
    Helixi222 – 2276
    Helixi231 – 2355
    Turni236 – 2383
    Helixi241 – 25212
    Helixi253 – 2564
    Helixi265 – 28016
    Helixi284 – 2863
    Helixi287 – 29913
    Helixi308 – 32316
    Helixi326 – 3283
    Helixi331 – 35424
    Turni365 – 3673
    Helixi370 – 38011
    Helixi382 – 39312
    Beta strandi396 – 3983
    Helixi401 – 41212
    Turni426 – 4283
    Helixi429 – 4324

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1LXWmodel-A104-433[»]
    ProteinModelPortaliQ9NP56.
    SMRiQ9NP56. Positions 100-416.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni172 – 410239CatalyticBy similarityAdd
    BLAST

    Domaini

    Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain.

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiNOG300643.
    HOGENOMiHOG000220881.
    HOVERGENiHBG053543.
    KOiK01120.
    OMAiNNTTHSG.
    OrthoDBiEOG7M98G3.
    PhylomeDBiQ9NP56.
    TreeFamiTF314638.

    Family and domain databases

    Gene3Di1.10.1300.10. 1 hit.
    InterProiIPR003607. HD/PDEase_dom.
    IPR023088. PDEase.
    IPR002073. PDEase_catalytic_dom.
    IPR023174. PDEase_CS.
    [Graphical view]
    PfamiPF00233. PDEase_I. 1 hit.
    [Graphical view]
    PRINTSiPR00387. PDIESTERASE1.
    SMARTiSM00471. HDc. 1 hit.
    [Graphical view]
    PROSITEiPS00126. PDEASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9NP56-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSCLMVERCG EILFENPDQN AKCVCMLGDI RLRGQTGVRA ERRGSYPFID    50
    FRLLNSTTYS GEIGTKKKVK RLLSFQRYFH ASRLLRGIIP QAPLHLLDED 100
    YLGQARHMLS KVGMWDFDIF LFDRLTNGNS LVTLLCHLFN THGLIHHFKL 150
    DMVTLHRFLV MVQEDYHSQN PYHNAVHAAD VTQAMHCYLK EPKLASFLTP 200
    LDIMLGLLAA AAHDVDHPGV NQPFLIKTNH HLANLYQNMS VLENHHWRST 250
    IGMLRESRLL AHLPKEMTQD IEQQLGSLIL ATDINRQNEF LTRLKAHLHN 300
    KDLRLEDAQD RHFMLQIALK CADICNPCRI WEMSKQWSER VCEEFYRQGE 350
    LEQKFELEIS PLCNQQKDSI PSIQIGFMSY IVEPLFREWA HFTGNSTLSE 400
    NMLGHLAHNK AQWKSLLPRQ HRSRGSSGSG PDHDHAGQGT ESEEQEGDSP 450
    Length:450
    Mass (Da):51,835
    Last modified:October 1, 2000 - v1
    Checksum:iEC142BF3E28D0028
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB038040 mRNA. Translation: BAA96537.1.
    AJ251860 mRNA. Translation: CAB92441.1.
    AL360178, AL133319, AL138828 Genomic DNA. Translation: CAH73075.1.
    AL133319, AL138828, AL360178 Genomic DNA. Translation: CAH73332.1.
    AL138828, AL133319, AL360178 Genomic DNA. Translation: CAI95287.1.
    CH471051 Genomic DNA. Translation: EAW47957.1.
    BC075082 mRNA. Translation: AAH75082.1.
    BC075083 mRNA. Translation: AAH75083.1.
    CCDSiCCDS5175.1.
    PIRiJC7266.
    RefSeqiNP_061818.1. NM_018945.3.
    UniGeneiHs.744230.

    Genome annotation databases

    EnsembliENST00000308191; ENSP00000310661; ENSG00000171408.
    GeneIDi27115.
    KEGGihsa:27115.
    UCSCiuc003qgp.3. human.

    Polymorphism databases

    DMDMi13626185.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB038040 mRNA. Translation: BAA96537.1 .
    AJ251860 mRNA. Translation: CAB92441.1 .
    AL360178 , AL133319 , AL138828 Genomic DNA. Translation: CAH73075.1 .
    AL133319 , AL138828 , AL360178 Genomic DNA. Translation: CAH73332.1 .
    AL138828 , AL133319 , AL360178 Genomic DNA. Translation: CAI95287.1 .
    CH471051 Genomic DNA. Translation: EAW47957.1 .
    BC075082 mRNA. Translation: AAH75082.1 .
    BC075083 mRNA. Translation: AAH75083.1 .
    CCDSi CCDS5175.1.
    PIRi JC7266.
    RefSeqi NP_061818.1. NM_018945.3.
    UniGenei Hs.744230.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1LXW model - A 104-433 [» ]
    ProteinModelPortali Q9NP56.
    SMRi Q9NP56. Positions 100-416.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 118010. 2 interactions.
    IntActi Q9NP56. 1 interaction.
    STRINGi 9606.ENSP00000310661.

    Chemistry

    BindingDBi Q9NP56.
    ChEMBLi CHEMBL2111411.
    DrugBanki DB00651. Dyphylline.
    DB00920. Ketotifen.
    GuidetoPHARMACOLOGYi 1306.

    PTM databases

    PhosphoSitei Q9NP56.

    Polymorphism databases

    DMDMi 13626185.

    Proteomic databases

    PaxDbi Q9NP56.
    PRIDEi Q9NP56.

    Protocols and materials databases

    DNASUi 27115.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000308191 ; ENSP00000310661 ; ENSG00000171408 .
    GeneIDi 27115.
    KEGGi hsa:27115.
    UCSCi uc003qgp.3. human.

    Organism-specific databases

    CTDi 27115.
    GeneCardsi GC06P136214.
    HGNCi HGNC:8792. PDE7B.
    HPAi HPA023967.
    MIMi 604645. gene.
    neXtProti NX_Q9NP56.
    PharmGKBi PA33140.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG300643.
    HOGENOMi HOG000220881.
    HOVERGENi HBG053543.
    KOi K01120.
    OMAi NNTTHSG.
    OrthoDBi EOG7M98G3.
    PhylomeDBi Q9NP56.
    TreeFami TF314638.

    Enzyme and pathway databases

    UniPathwayi UPA00762 ; UER00747 .
    Reactomei REACT_19327. G alpha (s) signalling events.

    Miscellaneous databases

    GenomeRNAii 27115.
    NextBioi 49800.
    PROi Q9NP56.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9NP56.
    CleanExi HS_PDE7B.
    Genevestigatori Q9NP56.

    Family and domain databases

    Gene3Di 1.10.1300.10. 1 hit.
    InterProi IPR003607. HD/PDEase_dom.
    IPR023088. PDEase.
    IPR002073. PDEase_catalytic_dom.
    IPR023174. PDEase_CS.
    [Graphical view ]
    Pfami PF00233. PDEase_I. 1 hit.
    [Graphical view ]
    PRINTSi PR00387. PDIESTERASE1.
    SMARTi SM00471. HDc. 1 hit.
    [Graphical view ]
    PROSITEi PS00126. PDEASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of human PDE7B, a cAMP-specific phosphodiesterase."
      Sasaki T., Kotera J., Yuasa K., Omori K.
      Biochem. Biophys. Res. Commun. 271:575-583(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    2. "Cloning and characterisation of the human and mouse PDE7B, a novel cAMP-specific nucleotide phosphodiesterase."
      Gardner C.E., Robas N.M., Cawkill D., Fidock M.D.
      Biochem. Biophys. Res. Commun. 272:186-192(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Fetal brain.
    3. "The DNA sequence and analysis of human chromosome 6."
      Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D.
      , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
      Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    6. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
      Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
      Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Leukemic T-cell.

    Entry informationi

    Entry nameiPDE7B_HUMAN
    AccessioniPrimary (citable) accession number: Q9NP56
    Secondary accession number(s): Q5W154
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 27, 2001
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 126 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 6
      Human chromosome 6: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    4. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3