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Protein

SIN3-HDAC complex-associated factor

Gene

SINHCAF

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Subunit of the Sin3 deacetylase complex (Sin3/HDAC), this subunit is important for the repression of genes encoding components of the TGF-beta signaling pathway (PubMed:22865885, PubMed:22984288). Core component of a SIN3A complex (composed of at least SINHCAF, SIN3A, HDAC1, SAP30, RBBP4, OGT and TET1) present in embryonic stem (ES) cells. Promotes the stability of SIN3A and its presence on chromatin and is essential for maintaining the potential of ES cells to proliferate rapidly, while ensuring a short G1-phase of the cell cycle, thereby preventing premature lineage priming (By similarity).By similarity2 Publications

GO - Biological processi

Names & Taxonomyi

Protein namesi
Recommended name:
SIN3-HDAC complex-associated factorImported
Alternative name(s):
Protein FAM60A
Tera protein homolog
Gene namesi
Name:SINHCAFImported
Synonyms:C12orf14, FAM60A
ORF Names:L4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiHostDB:ENSG00000139146.13
HGNCiHGNC:30702 SINHCAF
MIMi615027 gene
neXtProtiNX_Q9NP50

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi58516
OpenTargetsiENSG00000139146
PharmGKBiPA134921007

Polymorphism and mutation databases

BioMutaiFAM60A
DMDMi71151891

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001870861 – 221SIN3-HDAC complex-associated factorAdd BLAST221

Proteomic databases

EPDiQ9NP50
MaxQBiQ9NP50
PaxDbiQ9NP50
PeptideAtlasiQ9NP50
PRIDEiQ9NP50

PTM databases

iPTMnetiQ9NP50
PhosphoSitePlusiQ9NP50

Expressioni

Inductioni

Peaks during G1 and S phases of the cell cycle in U2OS cells. Up-regulated in squamous cell carcinoma (SCC), adenocarcinoma (AC), colon, ovary, rectum and stomach tumors.2 Publications

Gene expression databases

BgeeiENSG00000139146
CleanExiHS_FAM60A
ExpressionAtlasiQ9NP50 baseline and differential
GenevisibleiQ9NP50 HS

Organism-specific databases

HPAiHPA038167

Interactioni

Subunit structurei

Interacts with the Sin3/HDAC corepressor complex at least composed of BRMS1, BRMS1L, ING2, SAP30, SAP30L and HDAC1 (PubMed:22865885, PubMed:22984288). Found in a complex composed of at least SINHCAF, SIN3A, HDAC1, SAP30, RBBP4, OGT and TET1. Interacts with SIN3A and OGT (By similarity).By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
HDAC1Q135475EBI-741906,EBI-301834

Protein-protein interaction databases

BioGridi121842, 60 interactors
IntActiQ9NP50, 9 interactors
MINTiQ9NP50
STRINGi9606.ENSP00000337477

Structurei

3D structure databases

ProteinModelPortaliQ9NP50
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the SINHCAF family.Curated

Phylogenomic databases

eggNOGiENOG410IKPK Eukaryota
ENOG4111PRH LUCA
GeneTreeiENSGT00390000006485
HOVERGENiHBG053413
InParanoidiQ9NP50
OMAiCCSNKKS
OrthoDBiEOG091G0YQC
PhylomeDBiQ9NP50
TreeFamiTF323911

Family and domain databases

InterProiView protein in InterPro
IPR026065 FAM60A
PANTHERiPTHR13422 PTHR13422, 1 hit
PfamiView protein in Pfam
PF15396 FAM60A, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9NP50-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MFGFHKPKMY RSIEGCCICR AKSSSSRFTD SKRYEKDFQS CFGLHETRSG
60 70 80 90 100
DICNACVLLV KRWKKLPAGS KKNWNHVVDA RAGPSLKTTL KPKKVKTLSG
110 120 130 140 150
NRIKSNQISK LQKEFKRHNS DAHSTTSSAS PAQSPCYSNQ SDDGSDTEMA
160 170 180 190 200
SGSNRTPVFS FLDLTYWKRQ KICCGIIYKG RFGEVLIDTH LFKPCCSNKK
210 220
AAAEKPEEQG PEPLPISTQE W
Length:221
Mass (Da):24,852
Last modified:October 1, 2000 - v1
Checksum:iF5905C8AAC76AD45
GO
Isoform 2 (identifier: Q9NP50-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-148: Missing.

Show »
Length:73
Mass (Da):8,292
Checksum:i051702E1E409F11E
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_049027117R → H. Corresponds to variant dbSNP:rs2304459Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0147051 – 148Missing in isoform 2. 1 PublicationAdd BLAST148

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY598323 mRNA Translation: AAT06734.1
AF087885 mRNA Translation: AAP97184.1
AF212220 mRNA Translation: AAF87322.1
AK026932 mRNA Translation: BAB15592.1
AL157432 mRNA Translation: CAB75656.1
AC024940 Genomic DNA No translation available.
CH471116 Genomic DNA Translation: EAW88561.1
CH471116 Genomic DNA Translation: EAW88562.1
CH471116 Genomic DNA Translation: EAW88564.1
CH471116 Genomic DNA Translation: EAW88566.1
CH471116 Genomic DNA Translation: EAW88567.1
BC000024 mRNA Translation: AAH00024.1
BC004462 mRNA Translation: AAH04462.1
BC071966 mRNA Translation: AAH71966.1
CCDSiCCDS8723.1 [Q9NP50-1]
PIRiT46918
RefSeqiNP_001129283.1, NM_001135811.1 [Q9NP50-1]
NP_001129284.1, NM_001135812.1 [Q9NP50-1]
NP_067061.1, NM_021238.2 [Q9NP50-1]
XP_016875247.1, XM_017019758.1 [Q9NP50-1]
XP_016875248.1, XM_017019759.1 [Q9NP50-1]
XP_016875249.1, XM_017019760.1 [Q9NP50-1]
XP_016875250.1, XM_017019761.1 [Q9NP50-1]
XP_016875251.1, XM_017019762.1 [Q9NP50-1]
UniGeneiHs.505154

Genome annotation databases

EnsembliENST00000337682; ENSP00000337477; ENSG00000139146 [Q9NP50-1]
ENST00000454658; ENSP00000393279; ENSG00000139146 [Q9NP50-1]
ENST00000539409; ENSP00000443697; ENSG00000139146 [Q9NP50-2]
ENST00000542983; ENSP00000439952; ENSG00000139146 [Q9NP50-2]
ENST00000617411; ENSP00000479093; ENSG00000276371 [Q9NP50-1]
ENST00000631598; ENSP00000488852; ENSG00000276371 [Q9NP50-2]
ENST00000633702; ENSP00000488570; ENSG00000276371 [Q9NP50-1]
ENST00000633815; ENSP00000488406; ENSG00000276371 [Q9NP50-2]
GeneIDi58516
KEGGihsa:58516
UCSCiuc001rke.4 human [Q9NP50-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiSHCAF_HUMAN
AccessioniPrimary (citable) accession number: Q9NP50
Secondary accession number(s): D3DUV8, Q9BSZ8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: October 1, 2000
Last modified: May 23, 2018
This is version 126 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health