Q9NL89 (BGBP_BOMMO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Beta-1,3-glucan-binding protein Short name=BGBP Alternative name(s): Beta-1,3-glucan recognition protein Short name=BetaGRP
Organism	Bombyx mori (Silk moth)
Taxonomic identifier	7091 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Lepidoptera › Glossata › Ditrysia › Bombycoidea › Bombycidae › Bombycinae › Bombyx

Protein attributes

Sequence length	495 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the recognition of invading micro-organisms. Binds specifically to beta-1,3-glucan and activates the phenoloxidase cascade. Ref.1 Ref.2
Subunit structure	Monomer. Ref.2
Subcellular location	Secreted By similarity UniProtKB Q76DI2.
Tissue specificity	Hemocytes, fat body and epidermal cells. Ref.1
Induction	By bacterial and yeast infection. Ref.1
Post-translational modification	Glycosylated Probable. Ref.1
Sequence similarities	Belongs to the insect beta-1,3-glucan binding protein family.
Mass spectrometry	Molecular mass is 54594 Da from positions 17 - 495. Determined by MALDI. Ref.1

Ontologies

Keywords
Biological process	Immunity Innate immunity
Cellular component	Secreted
Domain	Signal
PTM	Glycoprotein
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro innate immune response Inferred from electronic annotation. Source: UniProtKB-KW regulation of innate immune response Inferred from direct assay Ref.1. Source: UniProtKB
Cellular component	extracellular region Inferred from direct assay Ref.1. Source: UniProtKB
Molecular function	bacterial cell surface binding Inferred from direct assay Ref.1. Source: UniProtKB hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 16

16

Ref.1

Chain

17 – 495

479

Beta-1,3-glucan-binding protein Ref.1

PRO_0000002816

Amino acid modifications

Glycosylation

378

1

N-linked (GlcNAc...) Probable

Secondary structure

1

.

.

.

.

.

.

.

.

.

495

Helix Strand Turn

Details...

Beta strand

33 – 36

4

Beta strand

44 – 47

4

Beta strand

64 – 68

5

Beta strand

92 – 95

4

Sequences

	Sequence		Length	Mass (Da)
	Q9NL89 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 09249039F7456BF2	FASTA	495	55,802
	10 20 30 40 50 60 MYKTCVWVLL FKIVLCYEAP PATLEAIHPK GLRVSVPDEG FSLFAFHGKL NEEMEGLEAG 70 80 90 100 110 120 HWSRDITKPK NGRWIFRDRN AALKIGDKIY FWTFVIKDGL GYRQDNGEWT VEGFVDEAGN 130 140 150 160 170 180 PVNTEGSEIT PGVEFTSTSL NPESPQSIPN QPPDNLPAKP PSEGYPCELS LSTVSVPGFV 190 200 210 220 230 240 CKGQLLFEDQ FNIPIHRGKI WVPEVKFPGE PDFPFNVYLS DNAEVNDGKL IIKPATLESK 250 260 270 280 290 300 YGEDFVRQSL DLSERCTGTV GTAQCLREAS GPLILPPIIT AKISTRHQFA FKYGRVEIRA 310 320 330 340 350 360 KMPKGDWLYP EILLEPRDNI YGVRNYASGI LKIASVKGNA EFSKKLYAGP IMTGSDPYRS 370 380 390 400 410 420 FYLKENIGYE SWNNDFHNYT LEWRPDGITL LVDGESYGEI KPGEGFYNVA NSYKVEAAPQ 430 440 450 460 470 480 WLKGTIMAPF DELFYVSIGL NVAGIREFSE DISNKPWKNS ATKAMLKFWD ARSQWFPTWD 490 EDSALQVDYV KVFAI

References

[1]	"A pattern-recognition protein for beta-1,3-glucan. The binding domain and the cDNA cloning of beta-1,3-glucan recognition protein from the silkworm, Bombyx mori." Ochiai M., Ashida M. J. Biol. Chem. 275:4995-5002(2000) [PubMed: 10671539] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 17-84; 98-159; 183-199; 241-274; 305-332; 346-389; 415-423 AND 464-495, FUNCTION, TISSUE SPECIFICITY, INDUCTION, MASS SPECTROMETRY. Strain: Kinshu X Showa. Tissue: Hemocyte and Larval hemolymph.
[2]	"Purification of a beta-1,3-glucan recognition protein in the prophenoloxidase activating system from hemolymph of the silkworm, Bombyx mori." Ochiai M., Ashida M. J. Biol. Chem. 263:12056-12062(1988) [PubMed: 3136171] [Abstract] Cited for: FUNCTION, SUBUNIT.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AB026441 mRNA. Translation: BAA92243.1.

RefSeq

NP_001036840.1. NM_001043375.1.

UniGene

Bmo.370.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2RQE	NMR	-	A	17-118	[»]
3AQX	X-ray	2.05	A/B	17-118	[»]

ProteinModelPortal

Q9NL89.

ModBase

Search...

Protein family/group databases

CAZy

CBM39. Carbohydrate-Binding Module Family 39.
GH16. Glycoside Hydrolase Family 16.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

692379.

Organism-specific databases

CTD

692379.

Family and domain databases

InterPro

IPR008985. ConA-like_lec_gl.
IPR013320. ConA-like_subgrp.
IPR000757. Glyco_hydro_16.
[Graphical view]

Gene3D

G3DSA:2.60.120.200. ConA_like_subgrp. 1 hit.

Pfam

PF00722. Glyco_hydro_16. 1 hit.
[Graphical view]

SUPFAM

SSF49899. ConA_like_lec_gl. 1 hit.

ProtoNet

Search...

Entry information

Entry name

BGBP_BOMMO

Accession

Primary (citable) accession number: Q9NL89

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2004
Last sequence update:	October 1, 2000
Last modified:	September 21, 2011
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)