Reviewed, UniProtKB/Swiss-Prot Q9NL89 (BGBP_BOMMO)

Names and origin

Protein names	Recommended name:     Beta-1,3-glucan-binding protein       Short name=BGBP Alternative name(s):     Beta-1,3-glucan recognition protein       Short name=BetaGRP
Organism	Bombyx mori (Silk moth)
Taxonomic identifier	7091 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Lepidoptera › Glossata › Ditrysia › Bombycoidea › Bombycidae › Bombycinae › Bombyx

Protein attributes

Sequence length	495 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in the recognition of invading micro-organisms. Binds specifically to beta-1,3-glucan and activates the phenoloxidase cascade. Ref.1 Ref.2
Subunit structure	Monomer. Ref.2
Subcellular location	Secreted By similarity. UniProtKB Q76DI2
Tissue specificity	Hemocytes, fat body and epidermal cells. Ref.1
Induction	By bacterial and yeast infection. Ref.1
Post-translational modification	Glycosylated Probable. Ref.1
Sequence similarities	Belongs to the insect beta-1,3-glucan binding protein family.
Mass spectrometry	Molecular mass is 54594 Da from positions 17 - 495. Determined by MALDI. Ref.1

Ontologies

Keywords
Biological process	Immune response Innate immunity
Cellular component	Secreted
Domain	Signal
PTM	Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro innate immune response Inferred from electronic annotation. Source: UniProtKB-KW regulation of innate immune response Ref.1 Inferred from direct assay. Source: UniProtKB
Cellular component	extracellular region Ref.1 Inferred from direct assay. Source: UniProtKB
Molecular function	bacterial binding Ref.1 Inferred from direct assay. Source: UniProtKB hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 16	16	Ref.1
	Chain	17 – 495	479	Beta-1,3-glucan-binding protein Ref.1		PRO_0000002816
Amino acid modifications
	Glycosylation	378	1	N-linked (GlcNAc...) Probable

Sequences

	Sequence		Length	Mass (Da)
	Q9NL89-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 09249039F7456BF2	FASTA	495	55,802
	10 20 30 40 50 60 MYKTCVWVLL FKIVLCYEAP PATLEAIHPK GLRVSVPDEG FSLFAFHGKL NEEMEGLEAG 70 80 90 100 110 120 HWSRDITKPK NGRWIFRDRN AALKIGDKIY FWTFVIKDGL GYRQDNGEWT VEGFVDEAGN 130 140 150 160 170 180 PVNTEGSEIT PGVEFTSTSL NPESPQSIPN QPPDNLPAKP PSEGYPCELS LSTVSVPGFV 190 200 210 220 230 240 CKGQLLFEDQ FNIPIHRGKI WVPEVKFPGE PDFPFNVYLS DNAEVNDGKL IIKPATLESK 250 260 270 280 290 300 YGEDFVRQSL DLSERCTGTV GTAQCLREAS GPLILPPIIT AKISTRHQFA FKYGRVEIRA 310 320 330 340 350 360 KMPKGDWLYP EILLEPRDNI YGVRNYASGI LKIASVKGNA EFSKKLYAGP IMTGSDPYRS 370 380 390 400 410 420 FYLKENIGYE SWNNDFHNYT LEWRPDGITL LVDGESYGEI KPGEGFYNVA NSYKVEAAPQ 430 440 450 460 470 480 WLKGTIMAPF DELFYVSIGL NVAGIREFSE DISNKPWKNS ATKAMLKFWD ARSQWFPTWD 490 EDSALQVDYV KVFAI

References

[1]

"A pattern-recognition protein for beta-1,3-glucan. The binding domain and the cDNA cloning of beta-1,3-glucan recognition protein from the silkworm, Bombyx mori." Ochiai M., Ashida M. J. Biol. Chem. 275:4995-5002(2000) [PubMed: 10671539] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 17-84; 98-159; 183-199; 241-274; 305-332; 346-389; 415-423 AND 464-495, FUNCTION, TISSUE SPECIFICITY, INDUCTION, MASS SPECTROMETRY. Strain: Kinshu X Showa. Tissue: Hemocyte and Larval hemolymph.

[2]

"Purification of a beta-1,3-glucan recognition protein in the prophenoloxidase activating system from hemolymph of the silkworm, Bombyx mori." Ochiai M., Ashida M. J. Biol. Chem. 263:12056-12062(1988) [PubMed: 3136171] [Abstract] Cited for: FUNCTION, SUBUNIT.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB026441 mRNA. Translation: BAA92243.1.
RefSeq	NP_001036840.1.
UniGene	Bmo.370
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	CBM39. Carbohydrate-Binding Module Family 39. GH16. Glycoside Hydrolase Family 16.
Genome annotation databases
GeneID	692379.
Family and domain databases
InterPro	IPR013320. ConA-like_subgrp. IPR000757. Glyco_hydro_16. [Graphical view]
Gene3D	G3DSA:2.60.120.200. ConA_like_subgrp. 1 hit.
Pfam	PF00722. Glyco_hydro_16. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BGBP_BOMMO

Accession

Primary (citable) accession number: Q9NL89

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2004
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)