Q9NKX1 (ENPL_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endoplasmin homolog

Alternative name(s):
92 kDa phosphoprotein
Glucose-regulated protein 94 homolog
Short name=GRP-94 homolog

Gene names

Name:	grp94
ORF Names:	DDB_G0280057

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	768 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May play a role in late differentiation as well as in starvation response. When overexpressed, suppresses the ability to form normal fruiting bodies and impairs prespore differentiation as well as maturation into spores.
Subcellular location	Endoplasmic reticulum. Golgi apparatus. Note: In prespore cells, preferentially localizes in Golgi vesicles and cisternae. Colocalizes with trap1 in the prespore-specific vacuole. Ref.1 Ref.3
Induction	Expression is greatly reduced following starvation.
Post-translational modification	Phosphorylated Potential.
Sequence similarities	Belongs to the heat shock protein 90 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Golgi apparatus
Domain	Signal
Ligand	ATP-binding Calcium Nucleotide-binding
Molecular function	Chaperone
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein folding Inferred from electronic annotation. Source: InterPro response to stress Inferred from electronic annotation. Source: InterPro
Cellular_component	Golgi-associated vesicle Inferred from direct assay Ref.3. Source: dictyBase endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 28	28	Potential
	Chain	29 – 768	740	Endoplasmin homolog		PRO_0000327690
Regions
	Motif	765 – 768	4	Prevents secretion from ER Potential
	Compositional bias	260 – 315	56	Glu-rich
	Compositional bias	283 – 287	5	Poly-Thr
Sites
	Binding site	83	1	ATP By similarity
	Binding site	127	1	ATP By similarity
	Binding site	140	1	ATP By similarity
	Binding site	173	1	ATP; via amide nitrogen By similarity
	Binding site	426	1	ATP By similarity
Amino acid modifications
	Glycosylation	83	1	N-linked (GlcNAc...) Potential
	Glycosylation	317	1	N-linked (GlcNAc...) Potential
	Glycosylation	423	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	309	1	T → I in BAA94290. Ref.1
	Sequence conflict	444 – 445	2	LV → G in EAL67255. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9NKX1 [UniParc]. Last modified April 29, 2008. Version 2. Checksum: 7EB40FDDC56059E0	FASTA	768	87,269
	10 20 30 40 50 60 MKSITKIFLI LGLFAFLLVA FAPSSSVATV NLESDGYTEA EAKLIEEKGE KFTFQTEVNK 70 80 90 100 110 120 LMNIIINSLY SKKEIFLREL ISNASDALDK IRFLALTNAD LLGEGEQSNL DIHIKIDKAN 130 140 150 160 170 180 NVLHITDRGV GMTKDELVRN LGTIAQSGTK EFIKKVSDSA ESSNLIGQFG VGFYSLFLVA 190 200 210 220 230 240 DSVVVTSKSN DDDQYVWTSD SQSSYTIAKD PKGNTLGRGT RISLHIKDDS KEFLDQEVIK 250 260 270 280 290 300 QLVKKYSQFI NFPIYLYVSE EVEIPKEEQE DSKPITDDQV EETTTTTEEG EEETTTEEEG 310 320 330 340 350 360 QTEEKKTKTV YKWEELNDSK PLWMKAAKDV TKEEYTEFFR SLSKTQDTPI TYSHFKTEGD 370 380 390 400 410 420 TEFRSILYIP ENPPSNMFDL EAAGSGLKLF VRRVFITDNL KELVPNWLRF LVGVIDSDDL 430 440 450 460 470 480 PLNVSREMLQ QNKILDAIKK KVILVKFISM IKELSEDEDK TKYNEFFKKF GSSMKLGAIE 490 500 510 520 530 540 DQANKKRLTK YLLFPSSKEE LTTFAGYVER MKEGQDQIYF ITGKSKDSVE ASPLIEQAIK 550 560 570 580 590 600 KGYEVLFLVD PIDEYLVPQL DKFDDKYKFT NLARSGVKFN EDKEEEDQRK QTAEEFKPLL 610 620 630 640 650 660 SYLKKTLSDK LEKVVISKVL ADSPSILVSN SWGVTANQER IMKAQAHQAN AQPQFNSKKI 670 680 690 700 710 720 MEINPSHPLI KKLLNRLNEF GEEDETTKVS AHVLYETSAL TAGYSIDNPT NFADFIYKLM 730 740 750 760 MINGDSLAQT NFETTKNENS GPSVSFGDDD ENQQQDFQQP PQSTHDEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Involvement of the glucose-regulated protein 94 (Dd-GRP94) in starvation response of Dictyostelium discoideum cells." Morita T., Saitoh K., Takagi T., Maeda Y. Biochem. Biophys. Res. Commun. 274:323-331(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[3]	"Changes in spatial and temporal localization of Dictyostelium homologues of TRAP1 and GRP94 revealed by immunoelectron microscopy." Yamaguchi H., Morita T., Amagai A., Maeda Y. Exp. Cell Res. 303:415-424(2005) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB040814 mRNA. Translation: BAA94290.2. AAFI02000035 Genomic DNA. Translation: EAL67255.1.
PIR	JC7352.
RefSeq	XP_641313.1. XM_636221.1.
3D structure databases
ProteinModelPortal	Q9NKX1.
SMR	Q9NKX1. Positions 42-260.
ModBase	Search...
Protein-protein interaction databases
STRING	44689.DDB_0215015.
Proteomic databases
PRIDE	Q9NKX1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	8622445.
KEGG	ddi:DDB_G0280057.
Organism-specific databases
dictyBase	DDB_G0280057. grp94.
Phylogenomic databases
eggNOG	COG0326.
ProtClustDB	CLSZ2846806.
Family and domain databases
Gene3D	3.30.565.10. 1 hit.
InterPro	IPR015566. Endoplasmin. IPR003594. HATPase_ATP-bd. IPR001404. Hsp90. IPR020575. Hsp90_N. IPR020568. Ribosomal_S5_D2-typ_fold. [Graphical view]
PANTHER	PTHR11528. PTHR11528. 1 hit. PTHR11528:SF21. PTHR11528:SF21. 1 hit.
Pfam	PF00183. HSP90. 1 hit. [Graphical view]
PIRSF	PIRSF002583. Hsp90. 1 hit.
PRINTS	PR00775. HEATSHOCK90.
SMART	SM00387. HATPase_c. 1 hit. [Graphical view]
SUPFAM	SSF55874. ATP_bd_ATPase. 1 hit. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
PROSITE	PS00014. ER_TARGET. 1 hit. PS00298. HSP90. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ENPL_DICDI

Accession

Primary (citable) accession number: Q9NKX1
Secondary accession number(s): Q54VP2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	April 29, 2008
Last modified:	May 29, 2013
This is version 77 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)