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Q9NKX1 (ENPL_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Endoplasmin homolog
Alternative name(s):
92 kDa phosphoprotein
Glucose-regulated protein 94 homolog
Short name=GRP-94 homolog
Gene names
Name:grp94
ORF Names:DDB_G0280057
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length768 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

May play a role in late differentiation as well as in starvation response. When overexpressed, suppresses the ability to form normal fruiting bodies and impairs prespore differentiation as well as maturation into spores. HAMAP-Rule MF_00505

Subcellular location

Endoplasmic reticulum. Golgi apparatus. Note: In prespore cells, preferentially localizes in Golgi vesicles and cisternae. Colocalizes with trap1 in the prespore-specific vacuole. Ref.1 Ref.3

Induction

Expression is greatly reduced following starvation. HAMAP-Rule MF_00505

Post-translational modification

Phosphorylated Potential. HAMAP-Rule MF_00505

Sequence similarities

Belongs to the heat shock protein 90 family.

Ontologies

Keywords
   Cellular componentEndoplasmic reticulum
Golgi apparatus
   DomainSignal
   LigandATP-binding
Calcium
Nucleotide-binding
   Molecular functionChaperone
   PTMGlycoprotein
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processprotein folding

Inferred from electronic annotation. Source: InterPro

response to stress

Inferred from electronic annotation. Source: InterPro

   Cellular_componentGolgi-associated vesicle

Inferred from direct assay Ref.3. Source: dictyBase

endoplasmic reticulum

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2828 Potential
Chain29 – 768740Endoplasmin homolog HAMAP-Rule MF_00505
PRO_0000327690

Regions

Motif765 – 7684Prevents secretion from ER Potential
Compositional bias260 – 31556Glu-rich HAMAP-Rule MF_00505
Compositional bias283 – 2875Poly-Thr HAMAP-Rule MF_00505

Sites

Binding site831ATP By similarity
Binding site1271ATP By similarity
Binding site1401ATP By similarity
Binding site1731ATP; via amide nitrogen By similarity
Binding site4261ATP By similarity

Amino acid modifications

Glycosylation831N-linked (GlcNAc...) Potential
Glycosylation3171N-linked (GlcNAc...) Potential
Glycosylation4231N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict3091T → I in BAA94290. Ref.1
Sequence conflict444 – 4452LV → G in EAL67255. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q9NKX1 [UniParc].

Last modified April 29, 2008. Version 2.
Checksum: 7EB40FDDC56059E0

FASTA76887,269
        10         20         30         40         50         60 
MKSITKIFLI LGLFAFLLVA FAPSSSVATV NLESDGYTEA EAKLIEEKGE KFTFQTEVNK 

        70         80         90        100        110        120 
LMNIIINSLY SKKEIFLREL ISNASDALDK IRFLALTNAD LLGEGEQSNL DIHIKIDKAN 

       130        140        150        160        170        180 
NVLHITDRGV GMTKDELVRN LGTIAQSGTK EFIKKVSDSA ESSNLIGQFG VGFYSLFLVA 

       190        200        210        220        230        240 
DSVVVTSKSN DDDQYVWTSD SQSSYTIAKD PKGNTLGRGT RISLHIKDDS KEFLDQEVIK 

       250        260        270        280        290        300 
QLVKKYSQFI NFPIYLYVSE EVEIPKEEQE DSKPITDDQV EETTTTTEEG EEETTTEEEG 

       310        320        330        340        350        360 
QTEEKKTKTV YKWEELNDSK PLWMKAAKDV TKEEYTEFFR SLSKTQDTPI TYSHFKTEGD 

       370        380        390        400        410        420 
TEFRSILYIP ENPPSNMFDL EAAGSGLKLF VRRVFITDNL KELVPNWLRF LVGVIDSDDL 

       430        440        450        460        470        480 
PLNVSREMLQ QNKILDAIKK KVILVKFISM IKELSEDEDK TKYNEFFKKF GSSMKLGAIE 

       490        500        510        520        530        540 
DQANKKRLTK YLLFPSSKEE LTTFAGYVER MKEGQDQIYF ITGKSKDSVE ASPLIEQAIK 

       550        560        570        580        590        600 
KGYEVLFLVD PIDEYLVPQL DKFDDKYKFT NLARSGVKFN EDKEEEDQRK QTAEEFKPLL 

       610        620        630        640        650        660 
SYLKKTLSDK LEKVVISKVL ADSPSILVSN SWGVTANQER IMKAQAHQAN AQPQFNSKKI 

       670        680        690        700        710        720 
MEINPSHPLI KKLLNRLNEF GEEDETTKVS AHVLYETSAL TAGYSIDNPT NFADFIYKLM 

       730        740        750        760 
MINGDSLAQT NFETTKNENS GPSVSFGDDD ENQQQDFQQP PQSTHDEL 

« Hide

References

« Hide 'large scale' references
[1]"Involvement of the glucose-regulated protein 94 (Dd-GRP94) in starvation response of Dictyostelium discoideum cells."
Morita T., Saitoh K., Takagi T., Maeda Y.
Biochem. Biophys. Res. Commun. 274:323-331(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION.
[2]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[3]"Changes in spatial and temporal localization of Dictyostelium homologues of TRAP1 and GRP94 revealed by immunoelectron microscopy."
Yamaguchi H., Morita T., Amagai A., Maeda Y.
Exp. Cell Res. 303:415-424(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB040814 mRNA. Translation: BAA94290.2.
AAFI02000035 Genomic DNA. Translation: EAL67255.1.
PIRJC7352.
RefSeqXP_641313.1. XM_636221.1.

3D structure databases

ProteinModelPortalQ9NKX1.
SMRQ9NKX1. Positions 42-260.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDB_0215015.

Proteomic databases

PRIDEQ9NKX1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID8622445.
KEGGddi:DDB_G0280057.

Organism-specific databases

dictyBaseDDB_G0280057. grp94.

Phylogenomic databases

eggNOGCOG0326.
PhylomeDBQ9NKX1.

Family and domain databases

Gene3D3.30.565.10. 1 hit.
HAMAPMF_00505. HSP90.
InterProIPR003594. HATPase_ATP-bd.
IPR001404. Hsp90_fam.
IPR020575. Hsp90_N.
IPR020568. Ribosomal_S5_D2-typ_fold.
[Graphical view]
PANTHERPTHR11528. PTHR11528. 1 hit.
PfamPF00183. HSP90. 1 hit.
[Graphical view]
PIRSFPIRSF002583. Hsp90. 1 hit.
PRINTSPR00775. HEATSHOCK90.
SMARTSM00387. HATPase_c. 1 hit.
[Graphical view]
SUPFAMSSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEPS00014. ER_TARGET. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameENPL_DICDI
AccessionPrimary (citable) accession number: Q9NKX1
Secondary accession number(s): Q54VP2
Entry history
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: April 29, 2008
Last modified: July 9, 2014
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase