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Q9NKX1

- ENPL_DICDI

UniProt

Q9NKX1 - ENPL_DICDI

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Protein
Endoplasmin homolog
Gene
grp94, DDB_G0280057
Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

May play a role in late differentiation as well as in starvation response. When overexpressed, suppresses the ability to form normal fruiting bodies and impairs prespore differentiation as well as maturation into spores.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei83 – 831ATP By similarity
Binding sitei127 – 1271ATP By similarity
Binding sitei140 – 1401ATP By similarity
Binding sitei173 – 1731ATP; via amide nitrogen By similarity
Binding sitei426 – 4261ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW

GO - Biological processi

  1. protein folding Source: InterPro
  2. response to stress Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Ligandi

ATP-binding, Calcium, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_218007. ATF6-alpha activates chaperone genes.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoplasmin homolog
Alternative name(s):
92 kDa phosphoprotein
Glucose-regulated protein 94 homolog
Short name:
GRP-94 homolog
Gene namesi
Name:grp94
ORF Names:DDB_G0280057
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 3, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0280057. grp94.

Subcellular locationi

Endoplasmic reticulum. Golgi apparatus
Note: In prespore cells, preferentially localizes in Golgi vesicles and cisternae. Colocalizes with trap1 in the prespore-specific vacuole.2 Publications

GO - Cellular componenti

  1. Golgi-associated vesicle Source: dictyBase
  2. endoplasmic reticulum Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2828 Reviewed prediction
Add
BLAST
Chaini29 – 768740Endoplasmin homologUniRule annotation
PRO_0000327690Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi83 – 831N-linked (GlcNAc...) Reviewed prediction
Glycosylationi317 – 3171N-linked (GlcNAc...) Reviewed prediction
Glycosylationi423 – 4231N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

Phosphorylated Reviewed prediction.UniRule annotation

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PRIDEiQ9NKX1.

Expressioni

Inductioni

Expression is greatly reduced following starvation.UniRule annotation

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB_0215015.

Structurei

3D structure databases

ProteinModelPortaliQ9NKX1.
SMRiQ9NKX1. Positions 42-260.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi765 – 7684Prevents secretion from ER Reviewed prediction

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi260 – 31556Glu-richUniRule annotation
Add
BLAST
Compositional biasi283 – 2875Poly-ThrUniRule annotation

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0326.
KOiK09487.
PhylomeDBiQ9NKX1.

Family and domain databases

Gene3Di3.30.565.10. 1 hit.
HAMAPiMF_00505. HSP90.
InterProiIPR003594. HATPase_ATP-bd.
IPR001404. Hsp90_fam.
IPR020575. Hsp90_N.
IPR020568. Ribosomal_S5_D2-typ_fold.
[Graphical view]
PANTHERiPTHR11528. PTHR11528. 1 hit.
PfamiPF00183. HSP90. 1 hit.
[Graphical view]
PIRSFiPIRSF002583. Hsp90. 1 hit.
PRINTSiPR00775. HEATSHOCK90.
SMARTiSM00387. HATPase_c. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEiPS00014. ER_TARGET. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9NKX1-1 [UniParc]FASTAAdd to Basket

« Hide

MKSITKIFLI LGLFAFLLVA FAPSSSVATV NLESDGYTEA EAKLIEEKGE    50
KFTFQTEVNK LMNIIINSLY SKKEIFLREL ISNASDALDK IRFLALTNAD 100
LLGEGEQSNL DIHIKIDKAN NVLHITDRGV GMTKDELVRN LGTIAQSGTK 150
EFIKKVSDSA ESSNLIGQFG VGFYSLFLVA DSVVVTSKSN DDDQYVWTSD 200
SQSSYTIAKD PKGNTLGRGT RISLHIKDDS KEFLDQEVIK QLVKKYSQFI 250
NFPIYLYVSE EVEIPKEEQE DSKPITDDQV EETTTTTEEG EEETTTEEEG 300
QTEEKKTKTV YKWEELNDSK PLWMKAAKDV TKEEYTEFFR SLSKTQDTPI 350
TYSHFKTEGD TEFRSILYIP ENPPSNMFDL EAAGSGLKLF VRRVFITDNL 400
KELVPNWLRF LVGVIDSDDL PLNVSREMLQ QNKILDAIKK KVILVKFISM 450
IKELSEDEDK TKYNEFFKKF GSSMKLGAIE DQANKKRLTK YLLFPSSKEE 500
LTTFAGYVER MKEGQDQIYF ITGKSKDSVE ASPLIEQAIK KGYEVLFLVD 550
PIDEYLVPQL DKFDDKYKFT NLARSGVKFN EDKEEEDQRK QTAEEFKPLL 600
SYLKKTLSDK LEKVVISKVL ADSPSILVSN SWGVTANQER IMKAQAHQAN 650
AQPQFNSKKI MEINPSHPLI KKLLNRLNEF GEEDETTKVS AHVLYETSAL 700
TAGYSIDNPT NFADFIYKLM MINGDSLAQT NFETTKNENS GPSVSFGDDD 750
ENQQQDFQQP PQSTHDEL 768
Length:768
Mass (Da):87,269
Last modified:April 29, 2008 - v2
Checksum:i7EB40FDDC56059E0
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti309 – 3091T → I in BAA94290. 1 Publication
Sequence conflicti444 – 4452LV → G in EAL67255. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB040814 mRNA. Translation: BAA94290.2.
AAFI02000035 Genomic DNA. Translation: EAL67255.1.
PIRiJC7352.
RefSeqiXP_641313.1. XM_636221.1.

Genome annotation databases

GeneIDi8622445.
KEGGiddi:DDB_G0280057.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB040814 mRNA. Translation: BAA94290.2 .
AAFI02000035 Genomic DNA. Translation: EAL67255.1 .
PIRi JC7352.
RefSeqi XP_641313.1. XM_636221.1.

3D structure databases

ProteinModelPortali Q9NKX1.
SMRi Q9NKX1. Positions 42-260.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0215015.

Proteomic databases

PRIDEi Q9NKX1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 8622445.
KEGGi ddi:DDB_G0280057.

Organism-specific databases

dictyBasei DDB_G0280057. grp94.

Phylogenomic databases

eggNOGi COG0326.
KOi K09487.
PhylomeDBi Q9NKX1.

Enzyme and pathway databases

Reactomei REACT_218007. ATF6-alpha activates chaperone genes.

Family and domain databases

Gene3Di 3.30.565.10. 1 hit.
HAMAPi MF_00505. HSP90.
InterProi IPR003594. HATPase_ATP-bd.
IPR001404. Hsp90_fam.
IPR020575. Hsp90_N.
IPR020568. Ribosomal_S5_D2-typ_fold.
[Graphical view ]
PANTHERi PTHR11528. PTHR11528. 1 hit.
Pfami PF00183. HSP90. 1 hit.
[Graphical view ]
PIRSFi PIRSF002583. Hsp90. 1 hit.
PRINTSi PR00775. HEATSHOCK90.
SMARTi SM00387. HATPase_c. 1 hit.
[Graphical view ]
SUPFAMi SSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEi PS00014. ER_TARGET. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Involvement of the glucose-regulated protein 94 (Dd-GRP94) in starvation response of Dictyostelium discoideum cells."
    Morita T., Saitoh K., Takagi T., Maeda Y.
    Biochem. Biophys. Res. Commun. 274:323-331(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION.
  2. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  3. "Changes in spatial and temporal localization of Dictyostelium homologues of TRAP1 and GRP94 revealed by immunoelectron microscopy."
    Yamaguchi H., Morita T., Amagai A., Maeda Y.
    Exp. Cell Res. 303:415-424(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiENPL_DICDI
AccessioniPrimary (citable) accession number: Q9NKX1
Secondary accession number(s): Q54VP2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: April 29, 2008
Last modified: September 3, 2014
This is version 85 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi