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Protein

Endoplasmin homolog

Gene

grp94

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

May play a role in late differentiation as well as in starvation response. When overexpressed, suppresses the ability to form normal fruiting bodies and impairs prespore differentiation as well as maturation into spores.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei83ATPBy similarity1
Binding sitei127ATPBy similarity1
Binding sitei140ATPBy similarity1
Binding sitei173ATP; via amide nitrogenBy similarity1
Binding sitei426ATPBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Ligandi

ATP-binding, Calcium, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Endoplasmin homolog
Alternative name(s):
92 kDa phosphoprotein
Glucose-regulated protein 94 homolog
Short name:
GRP-94 homolog
Gene namesi
Name:grp94
ORF Names:DDB_G0280057
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 3, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0280057. grp94.

Subcellular locationi

GO - Cellular componenti

  • endoplasmic reticulum Source: UniProtKB-SubCell
  • Golgi-associated vesicle Source: dictyBase
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 28Sequence analysisAdd BLAST28
ChainiPRO_000032769029 – 768Endoplasmin homologAdd BLAST740

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi83N-linked (GlcNAc...)Sequence analysis1
Glycosylationi317N-linked (GlcNAc...)Sequence analysis1
Glycosylationi423N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

Phosphorylated.Curated

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ9NKX1.
PRIDEiQ9NKX1.

Expressioni

Inductioni

Expression is greatly reduced following starvation.

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB0215015.

Structurei

3D structure databases

ProteinModelPortaliQ9NKX1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi765 – 768Prevents secretion from ERSequence analysis4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi260 – 315Glu-richAdd BLAST56
Compositional biasi283 – 287Poly-Thr5

Sequence similaritiesi

Belongs to the heat shock protein 90 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG0020. Eukaryota.
COG0326. LUCA.
InParanoidiQ9NKX1.
KOiK09487.
PhylomeDBiQ9NKX1.

Family and domain databases

Gene3Di3.30.565.10. 1 hit.
HAMAPiMF_00505. HSP90. 1 hit.
InterProiIPR003594. HATPase_C.
IPR001404. Hsp90_fam.
IPR020575. Hsp90_N.
IPR020568. Ribosomal_S5_D2-typ_fold.
[Graphical view]
PANTHERiPTHR11528. PTHR11528. 1 hit.
PfamiPF02518. HATPase_c. 1 hit.
PF00183. HSP90. 1 hit.
[Graphical view]
PIRSFiPIRSF002583. Hsp90. 1 hit.
PRINTSiPR00775. HEATSHOCK90.
SMARTiSM00387. HATPase_c. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEiPS00014. ER_TARGET. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9NKX1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSITKIFLI LGLFAFLLVA FAPSSSVATV NLESDGYTEA EAKLIEEKGE
60 70 80 90 100
KFTFQTEVNK LMNIIINSLY SKKEIFLREL ISNASDALDK IRFLALTNAD
110 120 130 140 150
LLGEGEQSNL DIHIKIDKAN NVLHITDRGV GMTKDELVRN LGTIAQSGTK
160 170 180 190 200
EFIKKVSDSA ESSNLIGQFG VGFYSLFLVA DSVVVTSKSN DDDQYVWTSD
210 220 230 240 250
SQSSYTIAKD PKGNTLGRGT RISLHIKDDS KEFLDQEVIK QLVKKYSQFI
260 270 280 290 300
NFPIYLYVSE EVEIPKEEQE DSKPITDDQV EETTTTTEEG EEETTTEEEG
310 320 330 340 350
QTEEKKTKTV YKWEELNDSK PLWMKAAKDV TKEEYTEFFR SLSKTQDTPI
360 370 380 390 400
TYSHFKTEGD TEFRSILYIP ENPPSNMFDL EAAGSGLKLF VRRVFITDNL
410 420 430 440 450
KELVPNWLRF LVGVIDSDDL PLNVSREMLQ QNKILDAIKK KVILVKFISM
460 470 480 490 500
IKELSEDEDK TKYNEFFKKF GSSMKLGAIE DQANKKRLTK YLLFPSSKEE
510 520 530 540 550
LTTFAGYVER MKEGQDQIYF ITGKSKDSVE ASPLIEQAIK KGYEVLFLVD
560 570 580 590 600
PIDEYLVPQL DKFDDKYKFT NLARSGVKFN EDKEEEDQRK QTAEEFKPLL
610 620 630 640 650
SYLKKTLSDK LEKVVISKVL ADSPSILVSN SWGVTANQER IMKAQAHQAN
660 670 680 690 700
AQPQFNSKKI MEINPSHPLI KKLLNRLNEF GEEDETTKVS AHVLYETSAL
710 720 730 740 750
TAGYSIDNPT NFADFIYKLM MINGDSLAQT NFETTKNENS GPSVSFGDDD
760
ENQQQDFQQP PQSTHDEL
Length:768
Mass (Da):87,269
Last modified:April 29, 2008 - v2
Checksum:i7EB40FDDC56059E0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti309T → I in BAA94290 (PubMed:10913338).Curated1
Sequence conflicti444 – 445LV → G in EAL67255 (PubMed:15875012).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB040814 mRNA. Translation: BAA94290.2.
AAFI02000035 Genomic DNA. Translation: EAL67255.1.
PIRiJC7352.
RefSeqiXP_641313.1. XM_636221.1.

Genome annotation databases

EnsemblProtistsiEAL67255; EAL67255; DDB_G0280057.
GeneIDi8622445.
KEGGiddi:DDB_G0280057.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB040814 mRNA. Translation: BAA94290.2.
AAFI02000035 Genomic DNA. Translation: EAL67255.1.
PIRiJC7352.
RefSeqiXP_641313.1. XM_636221.1.

3D structure databases

ProteinModelPortaliQ9NKX1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB0215015.

Proteomic databases

PaxDbiQ9NKX1.
PRIDEiQ9NKX1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiEAL67255; EAL67255; DDB_G0280057.
GeneIDi8622445.
KEGGiddi:DDB_G0280057.

Organism-specific databases

dictyBaseiDDB_G0280057. grp94.

Phylogenomic databases

eggNOGiKOG0020. Eukaryota.
COG0326. LUCA.
InParanoidiQ9NKX1.
KOiK09487.
PhylomeDBiQ9NKX1.

Miscellaneous databases

PROiQ9NKX1.

Family and domain databases

Gene3Di3.30.565.10. 1 hit.
HAMAPiMF_00505. HSP90. 1 hit.
InterProiIPR003594. HATPase_C.
IPR001404. Hsp90_fam.
IPR020575. Hsp90_N.
IPR020568. Ribosomal_S5_D2-typ_fold.
[Graphical view]
PANTHERiPTHR11528. PTHR11528. 1 hit.
PfamiPF02518. HATPase_c. 1 hit.
PF00183. HSP90. 1 hit.
[Graphical view]
PIRSFiPIRSF002583. Hsp90. 1 hit.
PRINTSiPR00775. HEATSHOCK90.
SMARTiSM00387. HATPase_c. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEiPS00014. ER_TARGET. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiENPL_DICDI
AccessioniPrimary (citable) accession number: Q9NKX1
Secondary accession number(s): Q54VP2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: April 29, 2008
Last modified: November 2, 2016
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.