Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q9NKX1

- ENPL_DICDI

UniProt

Q9NKX1 - ENPL_DICDI

Protein

Endoplasmin homolog

Gene

grp94

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 2 (29 Apr 2008)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    May play a role in late differentiation as well as in starvation response. When overexpressed, suppresses the ability to form normal fruiting bodies and impairs prespore differentiation as well as maturation into spores.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei83 – 831ATPBy similarity
    Binding sitei127 – 1271ATPBy similarity
    Binding sitei140 – 1401ATPBy similarity
    Binding sitei173 – 1731ATP; via amide nitrogenBy similarity
    Binding sitei426 – 4261ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW

    GO - Biological processi

    1. protein folding Source: InterPro
    2. response to stress Source: InterPro

    Keywords - Molecular functioni

    Chaperone

    Keywords - Ligandi

    ATP-binding, Calcium, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_218007. ATF6-alpha activates chaperone genes.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endoplasmin homolog
    Alternative name(s):
    92 kDa phosphoprotein
    Glucose-regulated protein 94 homolog
    Short name:
    GRP-94 homolog
    Gene namesi
    Name:grp94
    ORF Names:DDB_G0280057
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 3, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0280057. grp94.

    Subcellular locationi

    Endoplasmic reticulum. Golgi apparatus
    Note: In prespore cells, preferentially localizes in Golgi vesicles and cisternae. Colocalizes with trap1 in the prespore-specific vacuole.

    GO - Cellular componenti

    1. endoplasmic reticulum Source: UniProtKB-SubCell
    2. Golgi-associated vesicle Source: dictyBase

    Keywords - Cellular componenti

    Endoplasmic reticulum, Golgi apparatus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2828Sequence AnalysisAdd
    BLAST
    Chaini29 – 768740Endoplasmin homologPRO_0000327690Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi83 – 831N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi317 – 3171N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi423 – 4231N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    Phosphorylated.Curated

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    PRIDEiQ9NKX1.

    Expressioni

    Inductioni

    Expression is greatly reduced following starvation.

    Interactioni

    Protein-protein interaction databases

    STRINGi44689.DDB_0215015.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9NKX1.
    SMRiQ9NKX1. Positions 42-260.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi765 – 7684Prevents secretion from ERSequence Analysis

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi260 – 31556Glu-richAdd
    BLAST
    Compositional biasi283 – 2875Poly-Thr

    Sequence similaritiesi

    Belongs to the heat shock protein 90 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG0326.
    KOiK09487.
    PhylomeDBiQ9NKX1.

    Family and domain databases

    Gene3Di3.30.565.10. 1 hit.
    HAMAPiMF_00505. HSP90.
    InterProiIPR003594. HATPase_ATP-bd.
    IPR001404. Hsp90_fam.
    IPR020575. Hsp90_N.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    [Graphical view]
    PANTHERiPTHR11528. PTHR11528. 1 hit.
    PfamiPF00183. HSP90. 1 hit.
    [Graphical view]
    PIRSFiPIRSF002583. Hsp90. 1 hit.
    PRINTSiPR00775. HEATSHOCK90.
    SMARTiSM00387. HATPase_c. 1 hit.
    [Graphical view]
    SUPFAMiSSF54211. SSF54211. 1 hit.
    SSF55874. SSF55874. 1 hit.
    PROSITEiPS00014. ER_TARGET. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9NKX1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKSITKIFLI LGLFAFLLVA FAPSSSVATV NLESDGYTEA EAKLIEEKGE    50
    KFTFQTEVNK LMNIIINSLY SKKEIFLREL ISNASDALDK IRFLALTNAD 100
    LLGEGEQSNL DIHIKIDKAN NVLHITDRGV GMTKDELVRN LGTIAQSGTK 150
    EFIKKVSDSA ESSNLIGQFG VGFYSLFLVA DSVVVTSKSN DDDQYVWTSD 200
    SQSSYTIAKD PKGNTLGRGT RISLHIKDDS KEFLDQEVIK QLVKKYSQFI 250
    NFPIYLYVSE EVEIPKEEQE DSKPITDDQV EETTTTTEEG EEETTTEEEG 300
    QTEEKKTKTV YKWEELNDSK PLWMKAAKDV TKEEYTEFFR SLSKTQDTPI 350
    TYSHFKTEGD TEFRSILYIP ENPPSNMFDL EAAGSGLKLF VRRVFITDNL 400
    KELVPNWLRF LVGVIDSDDL PLNVSREMLQ QNKILDAIKK KVILVKFISM 450
    IKELSEDEDK TKYNEFFKKF GSSMKLGAIE DQANKKRLTK YLLFPSSKEE 500
    LTTFAGYVER MKEGQDQIYF ITGKSKDSVE ASPLIEQAIK KGYEVLFLVD 550
    PIDEYLVPQL DKFDDKYKFT NLARSGVKFN EDKEEEDQRK QTAEEFKPLL 600
    SYLKKTLSDK LEKVVISKVL ADSPSILVSN SWGVTANQER IMKAQAHQAN 650
    AQPQFNSKKI MEINPSHPLI KKLLNRLNEF GEEDETTKVS AHVLYETSAL 700
    TAGYSIDNPT NFADFIYKLM MINGDSLAQT NFETTKNENS GPSVSFGDDD 750
    ENQQQDFQQP PQSTHDEL 768
    Length:768
    Mass (Da):87,269
    Last modified:April 29, 2008 - v2
    Checksum:i7EB40FDDC56059E0
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti309 – 3091T → I in BAA94290. (PubMed:10913338)Curated
    Sequence conflicti444 – 4452LV → G in EAL67255. (PubMed:15875012)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB040814 mRNA. Translation: BAA94290.2.
    AAFI02000035 Genomic DNA. Translation: EAL67255.1.
    PIRiJC7352.
    RefSeqiXP_641313.1. XM_636221.1.

    Genome annotation databases

    GeneIDi8622445.
    KEGGiddi:DDB_G0280057.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB040814 mRNA. Translation: BAA94290.2 .
    AAFI02000035 Genomic DNA. Translation: EAL67255.1 .
    PIRi JC7352.
    RefSeqi XP_641313.1. XM_636221.1.

    3D structure databases

    ProteinModelPortali Q9NKX1.
    SMRi Q9NKX1. Positions 42-260.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDB_0215015.

    Proteomic databases

    PRIDEi Q9NKX1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 8622445.
    KEGGi ddi:DDB_G0280057.

    Organism-specific databases

    dictyBasei DDB_G0280057. grp94.

    Phylogenomic databases

    eggNOGi COG0326.
    KOi K09487.
    PhylomeDBi Q9NKX1.

    Enzyme and pathway databases

    Reactomei REACT_218007. ATF6-alpha activates chaperone genes.

    Family and domain databases

    Gene3Di 3.30.565.10. 1 hit.
    HAMAPi MF_00505. HSP90.
    InterProi IPR003594. HATPase_ATP-bd.
    IPR001404. Hsp90_fam.
    IPR020575. Hsp90_N.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    [Graphical view ]
    PANTHERi PTHR11528. PTHR11528. 1 hit.
    Pfami PF00183. HSP90. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF002583. Hsp90. 1 hit.
    PRINTSi PR00775. HEATSHOCK90.
    SMARTi SM00387. HATPase_c. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54211. SSF54211. 1 hit.
    SSF55874. SSF55874. 1 hit.
    PROSITEi PS00014. ER_TARGET. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Involvement of the glucose-regulated protein 94 (Dd-GRP94) in starvation response of Dictyostelium discoideum cells."
      Morita T., Saitoh K., Takagi T., Maeda Y.
      Biochem. Biophys. Res. Commun. 274:323-331(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION.
    2. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    3. "Changes in spatial and temporal localization of Dictyostelium homologues of TRAP1 and GRP94 revealed by immunoelectron microscopy."
      Yamaguchi H., Morita T., Amagai A., Maeda Y.
      Exp. Cell Res. 303:415-424(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiENPL_DICDI
    AccessioniPrimary (citable) accession number: Q9NKX1
    Secondary accession number(s): Q54VP2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 8, 2008
    Last sequence update: April 29, 2008
    Last modified: October 1, 2014
    This is version 86 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3