Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Peroxisomal multifunctional enzyme A

Gene

mfeA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Enzyme acting on the peroxisomal beta-oxidation pathway for fatty acids. Protects the cells from the increase of the harmful xenobiotic fatty acids incorporated from their diets and optimizes cellular lipid composition for proper development.1 Publication

Catalytic activityi

(S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH.

Pathway:ifatty acid beta-oxidation

This protein is involved in the pathway fatty acid beta-oxidation, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway fatty acid beta-oxidation and in Lipid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei19 – 191NAD; via amide nitrogenBy similarity
Binding sitei38 – 381NADBy similarity
Binding sitei97 – 971NAD; via carbonyl oxygenBy similarity
Binding sitei149 – 1491SubstrateBy similarity
Active sitei162 – 1621Proton acceptorBy similarity
Binding sitei412 – 4121SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi11 – 3525NADBy similarityAdd
BLAST
Nucleotide bindingi73 – 742NADBy similarity
Nucleotide bindingi162 – 1665NADBy similarity
Nucleotide bindingi194 – 1974NADBy similarity

GO - Molecular functioni

GO - Biological processi

  • cAMP-mediated signaling Source: dictyBase
  • fatty acid beta-oxidation Source: UniProtKB-UniPathway
  • sorocarp development Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

ReactomeiREACT_294321. Beta-oxidation of pristanoyl-CoA.
REACT_320074. Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol.
REACT_328800. alpha-linolenic acid (ALA) metabolism.
REACT_341516. Beta-oxidation of very long chain fatty acids.
UniPathwayiUPA00659.

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxisomal multifunctional enzyme A
Short name:
MFE-A
Alternative name(s):
MFE-1
Including the following 1 domains:
3-hydroxyacyl-CoA dehydrogenase (EC:1.1.1.35)
Gene namesi
Name:mfeA
Synonyms:mfe1
ORF Names:DDB_G0291247
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195 Componentsi: Chromosome 6, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0291247. mfeA.

Subcellular locationi

GO - Cellular componenti

  • peroxisome Source: dictyBase
Complete GO annotation...

Keywords - Cellular componenti

Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 441441Peroxisomal multifunctional enzyme APRO_0000328620Add
BLAST

Proteomic databases

PRIDEiQ9NKW1.

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB0201628.

Structurei

3D structure databases

ProteinModelPortaliQ9NKW1.
SMRiQ9NKW1. Positions 3-294.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini331 – 440110SCP2Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 3023023-hydroxyacyl-CoA dehydrogenaseAdd
BLAST

Sequence similaritiesi

Contains 1 SCP2 domain.Curated

Phylogenomic databases

eggNOGiCOG1028.
InParanoidiQ9NKW1.
KOiK12405.
OMAiMMANLQR.
PhylomeDBiQ9NKW1.

Family and domain databases

Gene3Di3.30.1050.10. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR003033. SCP2_sterol-bd_dom.
[Graphical view]
PfamiPF00106. adh_short. 1 hit.
PF02036. SCP2. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF55718. SSF55718. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9NKW1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALNFKDKVV IVTGAGGGIG KVYALEFAKR GAKVVVNDLG GSHTGQGSSS
60 70 80 90 100
KAADKVVEEI KAAGGTAVAN YDSVEDGEKI VQTAMDSFGG VDILINNAGI
110 120 130 140 150
LRDVSFGKMT DGDWDLVYRV HAKGAYKLSR AAWNHMREKN FGRIIMTSSA
160 170 180 190 200
AGLYGNFGQA NYGSMKMALV GLSNTLAQEG KSKNIHCNTI APIAASRLTE
210 220 230 240 250
SVMPPEILEQ MKPDYIVPLV LYLCHQDTTE TGGVFEVGAG WVSKVRLQRS
260 270 280 290 300
AGVYMKDLTP EKIKDNWAQI ESFDNPSYPT SASESVSGIL AAVNSKPADG
310 320 330 340 350
ESVLVRPPKV AVPKALAATP SGSVVVDGYN ASKIFTTIQG NIGAKGAELV
360 370 380 390 400
KKINGIYLIN IKKGTNTQAW ALDLKNGSGS IVVGAGSTKP NVTITVSDED
410 420 430 440
FVDIMTGKLN AQSAFTKGKL KISGNMGLAT KLGALMQGSK L
Length:441
Mass (Da):46,387
Last modified:October 1, 2000 - v1
Checksum:i2CEF3D9779D93BCA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB042104 mRNA. Translation: BAA94961.1.
AAFI02000177 Genomic DNA. Translation: EAL61607.1.
RefSeqiXP_635235.1. XM_630143.1.

Genome annotation databases

EnsemblProtistsiDDB0201628; DDB0201628; DDB_G0291247.
GeneIDi8628181.
KEGGiddi:DDB_G0291247.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB042104 mRNA. Translation: BAA94961.1.
AAFI02000177 Genomic DNA. Translation: EAL61607.1.
RefSeqiXP_635235.1. XM_630143.1.

3D structure databases

ProteinModelPortaliQ9NKW1.
SMRiQ9NKW1. Positions 3-294.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB0201628.

Proteomic databases

PRIDEiQ9NKW1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiDDB0201628; DDB0201628; DDB_G0291247.
GeneIDi8628181.
KEGGiddi:DDB_G0291247.

Organism-specific databases

dictyBaseiDDB_G0291247. mfeA.

Phylogenomic databases

eggNOGiCOG1028.
InParanoidiQ9NKW1.
KOiK12405.
OMAiMMANLQR.
PhylomeDBiQ9NKW1.

Enzyme and pathway databases

UniPathwayiUPA00659.
ReactomeiREACT_294321. Beta-oxidation of pristanoyl-CoA.
REACT_320074. Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol.
REACT_328800. alpha-linolenic acid (ALA) metabolism.
REACT_341516. Beta-oxidation of very long chain fatty acids.

Miscellaneous databases

PROiQ9NKW1.

Family and domain databases

Gene3Di3.30.1050.10. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR003033. SCP2_sterol-bd_dom.
[Graphical view]
PfamiPF00106. adh_short. 1 hit.
PF02036. SCP2. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF55718. SSF55718. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "MFE1, a member of the peroxisomal hydroxyacyl coenzyme A dehydrogenase family, affects fatty acid metabolism necessary for morphogenesis in Dictyostelium spp."
    Matsuoka S., Saito T., Kuwayama H., Morita N., Ochiai H., Maeda M.
    Eukaryot. Cell 2:638-645(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION.
    Strain: AX4.
  2. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.

Entry informationi

Entry nameiMFEA_DICDI
AccessioniPrimary (citable) accession number: Q9NKW1
Secondary accession number(s): Q54EK5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: October 1, 2000
Last modified: June 24, 2015
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.