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Q9NKW1

- MFEA_DICDI

UniProt

Q9NKW1 - MFEA_DICDI

Protein

Peroxisomal multifunctional enzyme A

Gene

mfeA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 82 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Enzyme acting on the peroxisomal beta-oxidation pathway for fatty acids. Protects the cells from the increase of the harmful xenobiotic fatty acids incorporated from their diets and optimizes cellular lipid composition for proper development.1 Publication

    Catalytic activityi

    (S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei19 – 191NAD; via amide nitrogenBy similarity
    Binding sitei38 – 381NADBy similarity
    Binding sitei97 – 971NAD; via carbonyl oxygenBy similarity
    Binding sitei149 – 1491SubstrateBy similarity
    Active sitei162 – 1621Proton acceptorBy similarity
    Binding sitei412 – 4121SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi11 – 3525NADBy similarityAdd
    BLAST
    Nucleotide bindingi73 – 742NADBy similarity
    Nucleotide bindingi162 – 1665NADBy similarity
    Nucleotide bindingi194 – 1974NADBy similarity

    GO - Molecular functioni

    1. 3-hydroxyacyl-CoA dehydrogenase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cAMP-mediated signaling Source: dictyBase
    2. fatty acid beta-oxidation Source: UniProtKB-UniPathway
    3. sorocarp development Source: dictyBase

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Fatty acid metabolism, Lipid metabolism

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    ReactomeiREACT_209874. Beta-oxidation of pristanoyl-CoA.
    REACT_209942. Beta-oxidation of very long chain fatty acids.
    REACT_221037. alpha-linolenic acid (ALA) metabolism.
    REACT_221982. Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol.
    UniPathwayiUPA00659.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Peroxisomal multifunctional enzyme A
    Short name:
    MFE-A
    Alternative name(s):
    MFE-1
    Including the following 1 domains:
    3-hydroxyacyl-CoA dehydrogenase (EC:1.1.1.35)
    Gene namesi
    Name:mfeA
    Synonyms:mfe1
    ORF Names:DDB_G0291247
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 6, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0291247. mfeA.

    Subcellular locationi

    Peroxisome 1 Publication

    GO - Cellular componenti

    1. peroxisome Source: dictyBase

    Keywords - Cellular componenti

    Peroxisome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 441441Peroxisomal multifunctional enzyme APRO_0000328620Add
    BLAST

    Proteomic databases

    PRIDEiQ9NKW1.

    Interactioni

    Protein-protein interaction databases

    STRINGi44689.DDB_0201628.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9NKW1.
    SMRiQ9NKW1. Positions 3-294.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini331 – 440110SCP2Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 3023023-hydroxyacyl-CoA dehydrogenaseAdd
    BLAST

    Sequence similaritiesi

    Contains 1 SCP2 domain.Curated

    Phylogenomic databases

    eggNOGiCOG1028.
    KOiK12405.
    OMAiHAKGAYK.
    PhylomeDBiQ9NKW1.

    Family and domain databases

    Gene3Di3.30.1050.10. 1 hit.
    3.40.50.720. 1 hit.
    InterProiIPR002198. DH_sc/Rdtase_SDR.
    IPR002347. Glc/ribitol_DH.
    IPR016040. NAD(P)-bd_dom.
    IPR003033. SCP2_sterol-bd_dom.
    [Graphical view]
    PfamiPF00106. adh_short. 1 hit.
    PF02036. SCP2. 1 hit.
    [Graphical view]
    PRINTSiPR00081. GDHRDH.
    PR00080. SDRFAMILY.
    SUPFAMiSSF55718. SSF55718. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9NKW1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MALNFKDKVV IVTGAGGGIG KVYALEFAKR GAKVVVNDLG GSHTGQGSSS    50
    KAADKVVEEI KAAGGTAVAN YDSVEDGEKI VQTAMDSFGG VDILINNAGI 100
    LRDVSFGKMT DGDWDLVYRV HAKGAYKLSR AAWNHMREKN FGRIIMTSSA 150
    AGLYGNFGQA NYGSMKMALV GLSNTLAQEG KSKNIHCNTI APIAASRLTE 200
    SVMPPEILEQ MKPDYIVPLV LYLCHQDTTE TGGVFEVGAG WVSKVRLQRS 250
    AGVYMKDLTP EKIKDNWAQI ESFDNPSYPT SASESVSGIL AAVNSKPADG 300
    ESVLVRPPKV AVPKALAATP SGSVVVDGYN ASKIFTTIQG NIGAKGAELV 350
    KKINGIYLIN IKKGTNTQAW ALDLKNGSGS IVVGAGSTKP NVTITVSDED 400
    FVDIMTGKLN AQSAFTKGKL KISGNMGLAT KLGALMQGSK L 441
    Length:441
    Mass (Da):46,387
    Last modified:October 1, 2000 - v1
    Checksum:i2CEF3D9779D93BCA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB042104 mRNA. Translation: BAA94961.1.
    AAFI02000177 Genomic DNA. Translation: EAL61607.1.
    RefSeqiXP_635235.1. XM_630143.1.

    Genome annotation databases

    EnsemblProtistsiDDB0201628; DDB0201628; DDB_G0291247.
    GeneIDi8628181.
    KEGGiddi:DDB_G0291247.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB042104 mRNA. Translation: BAA94961.1 .
    AAFI02000177 Genomic DNA. Translation: EAL61607.1 .
    RefSeqi XP_635235.1. XM_630143.1.

    3D structure databases

    ProteinModelPortali Q9NKW1.
    SMRi Q9NKW1. Positions 3-294.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDB_0201628.

    Proteomic databases

    PRIDEi Q9NKW1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0201628 ; DDB0201628 ; DDB_G0291247 .
    GeneIDi 8628181.
    KEGGi ddi:DDB_G0291247.

    Organism-specific databases

    dictyBasei DDB_G0291247. mfeA.

    Phylogenomic databases

    eggNOGi COG1028.
    KOi K12405.
    OMAi HAKGAYK.
    PhylomeDBi Q9NKW1.

    Enzyme and pathway databases

    UniPathwayi UPA00659 .
    Reactomei REACT_209874. Beta-oxidation of pristanoyl-CoA.
    REACT_209942. Beta-oxidation of very long chain fatty acids.
    REACT_221037. alpha-linolenic acid (ALA) metabolism.
    REACT_221982. Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol.

    Miscellaneous databases

    PROi Q9NKW1.

    Family and domain databases

    Gene3Di 3.30.1050.10. 1 hit.
    3.40.50.720. 1 hit.
    InterProi IPR002198. DH_sc/Rdtase_SDR.
    IPR002347. Glc/ribitol_DH.
    IPR016040. NAD(P)-bd_dom.
    IPR003033. SCP2_sterol-bd_dom.
    [Graphical view ]
    Pfami PF00106. adh_short. 1 hit.
    PF02036. SCP2. 1 hit.
    [Graphical view ]
    PRINTSi PR00081. GDHRDH.
    PR00080. SDRFAMILY.
    SUPFAMi SSF55718. SSF55718. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "MFE1, a member of the peroxisomal hydroxyacyl coenzyme A dehydrogenase family, affects fatty acid metabolism necessary for morphogenesis in Dictyostelium spp."
      Matsuoka S., Saito T., Kuwayama H., Morita N., Ochiai H., Maeda M.
      Eukaryot. Cell 2:638-645(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION.
      Strain: AX4.
    2. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.

    Entry informationi

    Entry nameiMFEA_DICDI
    AccessioniPrimary (citable) accession number: Q9NKW1
    Secondary accession number(s): Q54EK5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 8, 2008
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 82 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3