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Protein

Peroxisomal multifunctional enzyme A

Gene

mfeA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Enzyme acting on the peroxisomal beta-oxidation pathway for fatty acids. Protects the cells from the increase of the harmful xenobiotic fatty acids incorporated from their diets and optimizes cellular lipid composition for proper development.1 Publication

Catalytic activityi

(S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH.

Pathwayi: fatty acid beta-oxidation

This protein is involved in the pathway fatty acid beta-oxidation, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway fatty acid beta-oxidation and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei19NAD; via amide nitrogenBy similarity1
Binding sitei38NADBy similarity1
Binding sitei97NAD; via carbonyl oxygenBy similarity1
Binding sitei149SubstrateBy similarity1
Active sitei162Proton acceptorBy similarity1
Binding sitei412SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi11 – 35NADBy similarityAdd BLAST25
Nucleotide bindingi73 – 74NADBy similarity2
Nucleotide bindingi162 – 166NADBy similarity5
Nucleotide bindingi194 – 197NADBy similarity4

GO - Molecular functioni

GO - Biological processi

  • cAMP-mediated signaling Source: dictyBase
  • fatty acid beta-oxidation Source: UniProtKB-UniPathway
  • fatty acid metabolic process Source: dictyBase
  • sorocarp development Source: dictyBase

Keywordsi

Molecular functionOxidoreductase
Biological processFatty acid metabolism, Lipid metabolism
LigandNAD

Enzyme and pathway databases

ReactomeiR-DDI-193368 Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol
R-DDI-2046106 alpha-linolenic acid (ALA) metabolism
R-DDI-389887 Beta-oxidation of pristanoyl-CoA
R-DDI-390247 Beta-oxidation of very long chain fatty acids
UniPathwayiUPA00659

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxisomal multifunctional enzyme A
Short name:
MFE-A
Alternative name(s):
MFE-1
Including the following 1 domains:
3-hydroxyacyl-CoA dehydrogenase (EC:1.1.1.35)
Gene namesi
Name:mfeA
Synonyms:mfe1
ORF Names:DDB_G0291247
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyostelidsDictyostelialesDictyosteliaceaeDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 6, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0291247 mfeA

Subcellular locationi

GO - Cellular componenti

  • peroxisome Source: dictyBase

Keywords - Cellular componenti

Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003286201 – 441Peroxisomal multifunctional enzyme AAdd BLAST441

Proteomic databases

PaxDbiQ9NKW1

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB0201628

Structurei

3D structure databases

ProteinModelPortaliQ9NKW1
SMRiQ9NKW1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini331 – 440SCP2Add BLAST110

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 3023-hydroxyacyl-CoA dehydrogenaseAdd BLAST302

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410IG9H Eukaryota
ENOG410ZVEX LUCA
InParanoidiQ9NKW1
KOiK12405
OMAiQEMADGV
PhylomeDBiQ9NKW1

Family and domain databases

Gene3Di3.30.1050.10, 1 hit
InterProiView protein in InterPro
IPR036291 NAD(P)-bd_dom_sf
IPR003033 SCP2_sterol-bd_dom
IPR036527 SCP2_sterol-bd_dom_sf
IPR002347 SDR_fam
PfamiView protein in Pfam
PF00106 adh_short, 1 hit
PF02036 SCP2, 1 hit
PRINTSiPR00081 GDHRDH
PR00080 SDRFAMILY
SUPFAMiSSF51735 SSF51735, 1 hit
SSF55718 SSF55718, 1 hit

Sequencei

Sequence statusi: Complete.

Q9NKW1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALNFKDKVV IVTGAGGGIG KVYALEFAKR GAKVVVNDLG GSHTGQGSSS
60 70 80 90 100
KAADKVVEEI KAAGGTAVAN YDSVEDGEKI VQTAMDSFGG VDILINNAGI
110 120 130 140 150
LRDVSFGKMT DGDWDLVYRV HAKGAYKLSR AAWNHMREKN FGRIIMTSSA
160 170 180 190 200
AGLYGNFGQA NYGSMKMALV GLSNTLAQEG KSKNIHCNTI APIAASRLTE
210 220 230 240 250
SVMPPEILEQ MKPDYIVPLV LYLCHQDTTE TGGVFEVGAG WVSKVRLQRS
260 270 280 290 300
AGVYMKDLTP EKIKDNWAQI ESFDNPSYPT SASESVSGIL AAVNSKPADG
310 320 330 340 350
ESVLVRPPKV AVPKALAATP SGSVVVDGYN ASKIFTTIQG NIGAKGAELV
360 370 380 390 400
KKINGIYLIN IKKGTNTQAW ALDLKNGSGS IVVGAGSTKP NVTITVSDED
410 420 430 440
FVDIMTGKLN AQSAFTKGKL KISGNMGLAT KLGALMQGSK L
Length:441
Mass (Da):46,387
Last modified:October 1, 2000 - v1
Checksum:i2CEF3D9779D93BCA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB042104 mRNA Translation: BAA94961.1
AAFI02000177 Genomic DNA Translation: EAL61607.1
RefSeqiXP_635235.1, XM_630143.1

Genome annotation databases

EnsemblProtistsiEAL61607; EAL61607; DDB_G0291247
GeneIDi8628181
KEGGiddi:DDB_G0291247

Similar proteinsi

Entry informationi

Entry nameiMFEA_DICDI
AccessioniPrimary (citable) accession number: Q9NKW1
Secondary accession number(s): Q54EK5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: October 1, 2000
Last modified: March 28, 2018
This is version 102 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health