Reviewed, UniProtKB/Swiss-Prot Q9NKV0 (MLF_DROME)

Names and origin

Protein names

Recommended name:
    Myeloid leukemia factor
Alternative name(s):
    Myelodysplasia-myeloid leukemia factor
      Short name=dMLF

Gene names

Name:	Mlf
ORF Names:	CG8295

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	376 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Subunit structure	Interacts with DRE-binding factor Dref. Ref.1
Subcellular location	Cytoplasm. Ref.1
Tissue specificity	Expressed at high levels in unfertilized eggs, early embryos, pupae and adult males while a low level expression is found in adult females and larvae. Ref.1
Developmental stage	High levels are seen in unfertilized eggs and expression increases slightly during early embryo stages (2-3 hours). Levels are high in embryos until 4 hours after fertilization and then decrease gradually through embryonic and larval stages. Ref.1
Sequence similarities	Belongs to the MLF family.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	nucleus Inferred from direct assay. Source: FlyBase perinuclear region of cytoplasm Inferred from direct assay. Source: FlyBase polytene chromosome interband Inferred from direct assay. Source: FlyBase
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform D (identifier: Q9NKV0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform A (identifier: Q9NKV0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     271-337: Missing.

Isoform B (identifier: Q9NKV0-3)

The sequence of this isoform differs from the canonical sequence as follows:
     270-273: SGNI → RYNY
     274-376: Missing.

Isoform C (identifier: Q9NKV0-4)

The sequence of this isoform differs from the canonical sequence as follows:
     2-19: SLFGALMGDFDDDLGLMN → KQKRKSKSNRSQMELH

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 376	376	Myeloid leukemia factor		PRO_0000220756
Regions
	Region	96 – 202	107	Interaction with DREF
	Compositional bias	236 – 243	8	Poly-Asp
Amino acid modifications
	Modified residue	323	1	Phosphothreonine Ref.6
Natural variations
	Alternative sequence	2 – 19	18	SLFGA…LGLMN → KQKRKSKSNRSQMELH in isoform C.		VSP_026174
	Alternative sequence	270 – 273	4	SGNI → RYNY in isoform B.		VSP_026175
	Alternative sequence	271 – 337	67	Missing in isoform A.		VSP_026176
	Alternative sequence	274 – 376	103	Missing in isoform B.		VSP_026177
Experimental info
	Sequence conflict	139	1	R → K in AAN71684. Ref.5
	Sequence conflict	262	1	S → T in AAN71684. Ref.5
	Sequence conflict	357	1	F → Y in AAN71684. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Isoform D [UniParc]. Last modified June 26, 2007. Version 2. Checksum: AD61C1CBD15AD0D0	FASTA	376	41,377
	10 20 30 40 50 60 MSLFGALMGD FDDDLGLMNN HMNHTMNAMN MQMRSMNRLM NSFMPDPFMQ VSPFDQGFQQ 70 80 90 100 110 120 NALMERPQMP AMPAMGLFGM PMMPNFNRLL NADIGGNSGA SFCQSTVMTM SSGPDGRPQI 130 140 150 160 170 180 YQASTSTKTG PGGVRETRRT VQDSRTGVKK MAIGHHIGER AHIIEKEQDM RSGQLEERQE 190 200 210 220 230 240 FINLEEGEAE QFDREFTSRA SRGAVQSRHH AGGMQAIMPA RPAAHTSTLT IEPVEDDDDD 250 260 270 280 290 300 DDDCVIQEQQ PVRSSAGRHY SSAPTAPQNS GNIATAAAPT PTSSPTTYDT SNGNNNNYVS 310 320 330 340 350 360 SRRSYLRNGH GHSLATPRRP LRTPPSSPLA TVSTSPSIHP HPYAANPRRQ QRAVKHFHTE 370 DEAASSYKAV KRGKKK
	Isoform A. Checksum: 4C50951D548FD9AD	FASTA	309	34,414
	10 20 30 40 50 60 MSLFGALMGD FDDDLGLMNN HMNHTMNAMN MQMRSMNRLM NSFMPDPFMQ VSPFDQGFQQ 70 80 90 100 110 120 NALMERPQMP AMPAMGLFGM PMMPNFNRLL NADIGGNSGA SFCQSTVMTM SSGPDGRPQI 130 140 150 160 170 180 YQASTSTKTG PGGVRETRRT VQDSRTGVKK MAIGHHIGER AHIIEKEQDM RSGQLEERQE 190 200 210 220 230 240 FINLEEGEAE QFDREFTSRA SRGAVQSRHH AGGMQAIMPA RPAAHTSTLT IEPVEDDDDD 250 260 270 280 290 300 DDDCVIQEQQ PVRSSAGRHY SSAPTAPQNS IHPHPYAANP RRQQRAVKHF HTEDEAASSY KAVKRGKKK
	Isoform B. Checksum: FC4DEAE1AF77A9B1	FASTA	273	30,402
	10 20 30 40 50 60 MSLFGALMGD FDDDLGLMNN HMNHTMNAMN MQMRSMNRLM NSFMPDPFMQ VSPFDQGFQQ 70 80 90 100 110 120 NALMERPQMP AMPAMGLFGM PMMPNFNRLL NADIGGNSGA SFCQSTVMTM SSGPDGRPQI 130 140 150 160 170 180 YQASTSTKTG PGGVRETRRT VQDSRTGVKK MAIGHHIGER AHIIEKEQDM RSGQLEERQE 190 200 210 220 230 240 FINLEEGEAE QFDREFTSRA SRGAVQSRHH AGGMQAIMPA RPAAHTSTLT IEPVEDDDDD 250 260 270 DDDCVIQEQQ PVRSSAGRHY SSAPTAPQNR YNY
	Isoform C. Checksum: 0AACAEBA9F00D790	FASTA	374	41,431
	10 20 30 40 50 60 MKQKRKSKSN RSQMELHNHM NHTMNAMNMQ MRSMNRLMNS FMPDPFMQVS PFDQGFQQNA 70 80 90 100 110 120 LMERPQMPAM PAMGLFGMPM MPNFNRLLNA DIGGNSGASF CQSTVMTMSS GPDGRPQIYQ 130 140 150 160 170 180 ASTSTKTGPG GVRETRRTVQ DSRTGVKKMA IGHHIGERAH IIEKEQDMRS GQLEERQEFI 190 200 210 220 230 240 NLEEGEAEQF DREFTSRASR GAVQSRHHAG GMQAIMPARP AAHTSTLTIE PVEDDDDDDD 250 260 270 280 290 300 DCVIQEQQPV RSSAGRHYSS APTAPQNSGN IATAAAPTPT SSPTTYDTSN GNNNNYVSSR 310 320 330 340 350 360 RSYLRNGHGH SLATPRRPLR TPPSSPLATV STSPSIHPHP YAANPRRQQR AVKHFHTEDE 370 AASSYKAVKR GKKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of a Drosophila homologue of the human myelodysplasia/myeloid leukemia factor (MLF)." Ohno K., Takahashi Y., Hirose F., Inoue Y.H., Taguchi O., Nishida Y., Matsukage A., Yamaguchi M. Gene 260:133-143(2000) [PubMed: 11137299] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), INTERACTION WITH DREF, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[2]	"Suppression of polyglutamine toxicity by a Drosophila homolog of myeloid leukemia factor 1." Kazemi-Esfarjani P., Benzer S. Hum. Mol. Genet. 11:2657-2672(2002) [PubMed: 12354791] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B). Tissue: Head.
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
[5]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A). Strain: Berkeley. Tissue: Embryo.
[6]	"Phosphoproteome analysis of Drosophila melanogaster embryos." Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P. J. Proteome Res. 7:1675-1682(2008) [PubMed: 18327897] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-323, MASS SPECTROMETRY. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB043986 mRNA. Translation: BAA96391.1. AY037049 mRNA. Translation: AAK63191.1. AE013599 Genomic DNA. Translation: AAF58093.2. AE013599 Genomic DNA. Translation: AAS64849.1. AE013599 Genomic DNA. Translation: AAM70968.2. AE013599 Genomic DNA. Translation: AAM70969.1. AY118583 mRNA. Translation: AAM49952.1. BT001895 mRNA. Translation: AAN71684.1.
RefSeq	NP_523753.1. NP_725501.2. NP_725502.1. NP_995847.1.
UniGene	Dm.12915
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9NKV0. 7 interactions.
Proteomic databases
PRIDE	Q9NKV0.
Genome annotation databases
Ensembl	FBgn0034051. Drosophila melanogaster. [Contig view]
GeneID	36750.
KEGG	dme:Dmel_CG8295.
Organism-specific databases
FlyBase	FBgn0034051. Mlf.
Phylogenomic databases
OMA	Q9NKV0. ASFCQST.
Enzyme and pathway databases
BioCyc	DMEL-XXX-02:DMEL-XXX-02-004834-MON.
Gene expression databases
ArrayExpress	Q9NKV0.
GermOnline	CG8295. Drosophila melanogaster.
Family and domain databases
InterPro	IPR019376. M-m_leukemia_factor_1-interact. [Graphical view]
Pfam	PF10248. Mlf1IP. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	800181.

Entry information

Entry name

MLF_DROME

Accession

Primary (citable) accession number: Q9NKV0
Secondary accession number(s): A1ZA61 , A1ZA62, Q540Y3, Q8IG90, Q95P43, Q9V7G3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 2, 2001
Last sequence update:	June 26, 2007
Last modified:	June 16, 2009
This is version 56 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project