Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Calcium-dependent protein kinase 3

Gene

CPK3

Organism
Plasmodium falciparum (isolate 3D7)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Calcium-dependent kinase that may play a role in the sexual reproduction and mosquito transmission of P.falciparum.By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by calcium.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei147 – 1471ATPPROSITE-ProRule annotation
Active sitei238 – 2381Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi124 – 1329ATPPROSITE-ProRule annotation
Calcium bindingi431 – 442121PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi466 – 477122PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi501 – 512123PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi542 – 553124PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Differentiation

Keywords - Ligandi

ATP-binding, Calcium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-dependent protein kinase 3 (EC:2.7.11.1)
Alternative name(s):
PfCDPK3
Gene namesi
Name:CPK3
Synonyms:CDPK3
ORF Names:MAL3P3.17, PFC0420w
OrganismiPlasmodium falciparum (isolate 3D7)
Taxonomic identifieri36329 [NCBI]
Taxonomic lineageiEukaryotaAlveolataApicomplexaAconoidasidaHaemosporidaPlasmodiidaePlasmodiumPlasmodium (Laverania)
Proteomesi
  • UP000001450 Componenti: Chromosome 3

Organism-specific databases

EuPathDBiPlasmoDB:PF3D7_0310100.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 562562Calcium-dependent protein kinase 3PRO_0000085839Add
BLAST

Expressioni

Developmental stagei

Expressed in sexual stages, but not in asexual stages.1 Publication

Interactioni

GO - Molecular functioni

Structurei

Secondary structure

1
562
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi388 – 41427Combined sources
Helixi418 – 43013Combined sources
Beta strandi435 – 4384Combined sources
Helixi440 – 44910Combined sources
Helixi458 – 4658Combined sources
Beta strandi470 – 4745Combined sources
Helixi475 – 4828Combined sources
Helixi485 – 4873Combined sources
Helixi490 – 50011Combined sources
Helixi510 – 51910Combined sources
Beta strandi520 – 5223Combined sources
Helixi528 – 54114Combined sources
Beta strandi543 – 5497Combined sources
Helixi551 – 5588Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3K21X-ray1.15A388-560[»]
ProteinModelPortaliQ9NJU9.
SMRiQ9NJU9. Positions 105-560.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9NJU9.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini118 – 373256Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini418 – 45336EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini453 – 48735EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini488 – 52336EF-hand 3PROSITE-ProRule annotationAdd
BLAST
Domaini529 – 56234EF-hand 4PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.PROSITE-ProRule annotation
Contains 4 EF-hand domains.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

HOGENOMiHOG000233030.
InParanoidiQ9NJU9.
KOiK13412.
OMAiSTGCEYA.
PhylomeDBiQ9NJU9.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF13499. EF-hand_7. 2 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 4 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00018. EF_HAND_1. 3 hits.
PS50222. EF_HAND_2. 4 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9NJU9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNDLIIKNNK KGSCDVIIKY KCKKSDENIK RRKSSHKYIK NKSVVLGRSI
60 70 80 90 100
MTNKKEKLKG ALKYKGSKKE IKICNKKSMI KNDKDENTTL KSMKSDNFKF
110 120 130 140 150
SRRGFILSFT GNLEDFYNLS KEPLGKGTYG CVYKATDKLL KISRAVKVVS
160 170 180 190 200
KKKLKNIPRF RQEIDIMKNL DHPNVVKLLE TFEDSNQIYL VMELCTGGEL
210 220 230 240 250
FDKIVKKGCF VETFASFIMK QIFSVLNYLH IRNICHRDIK PENFLFYDMT
260 270 280 290 300
PESLIKIIDF GLASYFTHNN YEMKTKAGTP YYVAPQVLTG SYNYKCDMWS
310 320 330 340 350
SGVLFYILLC GYPPFFGESD HEILSMVKKG KYQFKGKEWN NISEEAKDLI
360 370 380 390 400
KRCLTMDADK RICASEALQH PWFKKKKYAF NMDMKMDIHV LENFKNYGLL
410 420 430 440 450
LKFQKLAMTI IAQQSNDYDV EKLKSTFLVL DEDGKGYITK EQLKKGLEKD
460 470 480 490 500
GLKLPYNFDL LLDQIDSDGS GKIDYTEFIA AALDRKQLSK KLIYCAFRVF
510 520 530 540 550
DVDNDGEITT AELAHILYNG NKKGNITQRD VNRVKRMIRD VDKNNDGKID
560
FHEFSEMMKL KF
Length:562
Mass (Da):65,288
Last modified:October 1, 2000 - v1
Checksum:i80DB5BA4194AC2E3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF106064 Genomic DNA. Translation: AAF63154.1.
AL844502 Genomic DNA. Translation: CAB11118.4.
PIRiT18445.
RefSeqiXP_001351174.1. XM_001351138.1.

Genome annotation databases

EnsemblProtistsiPFC0420w:mRNA; PFC0420w:pep; PFC0420w.
GeneDBiPF3D7_0310100.1:pep.
GeneIDi814416.
KEGGipfa:PFC0420w.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF106064 Genomic DNA. Translation: AAF63154.1.
AL844502 Genomic DNA. Translation: CAB11118.4.
PIRiT18445.
RefSeqiXP_001351174.1. XM_001351138.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3K21X-ray1.15A388-560[»]
ProteinModelPortaliQ9NJU9.
SMRiQ9NJU9. Positions 105-560.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiPFC0420w:mRNA; PFC0420w:pep; PFC0420w.
GeneDBiPF3D7_0310100.1:pep.
GeneIDi814416.
KEGGipfa:PFC0420w.

Organism-specific databases

EuPathDBiPlasmoDB:PF3D7_0310100.

Phylogenomic databases

HOGENOMiHOG000233030.
InParanoidiQ9NJU9.
KOiK13412.
OMAiSTGCEYA.
PhylomeDBiQ9NJU9.

Miscellaneous databases

EvolutionaryTraceiQ9NJU9.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF13499. EF-hand_7. 2 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 4 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00018. EF_HAND_1. 3 hits.
PS50222. EF_HAND_2. 4 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sexual stage-specific expression of a third calcium-dependent protein kinase from Plasmodium falciparum."
    Li J.-L., Baker D.A., Cox L.S.
    Biochim. Biophys. Acta 1491:341-349(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], POSSIBLE FUNCTION, DEVELOPMENTAL STAGE.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Isolate 3D7.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Isolate 3D7.
  4. "Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13."
    Hall N., Pain A., Berriman M., Churcher C.M., Harris B., Harris D., Mungall K.L., Bowman S., Atkin R., Baker S., Barron A., Brooks K., Buckee C.O., Burrows C., Cherevach I., Chillingworth C., Chillingworth T., Christodoulou Z.
    , Clark L., Clark R., Corton C., Cronin A., Davies R.M., Davis P., Dear P., Dearden F., Doggett J., Feltwell T., Goble A., Goodhead I., Gwilliam R., Hamlin N., Hance Z., Harper D., Hauser H., Hornsby T., Holroyd S., Horrocks P., Humphray S., Jagels K., James K.D., Johnson D., Kerhornou A., Knights A., Konfortov B., Kyes S., Larke N., Lawson D., Lennard N., Line A., Maddison M., Mclean J., Mooney P., Moule S., Murphy L., Oliver K., Ormond D., Price C., Quail M.A., Rabbinowitsch E., Rajandream M.A., Rutter S., Rutherford K.M., Sanders M., Simmonds M., Seeger K., Sharp S., Smith R., Squares R., Squares S., Stevens K., Taylor K., Tivey A., Unwin L., Whitehead S., Woodward J.R., Sulston J.E., Craig A., Newbold C., Barrell B.G.
    Nature 419:527-531(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Isolate 3D7.

Entry informationi

Entry nameiCDPK3_PLAF7
AccessioniPrimary (citable) accession number: Q9NJU9
Secondary accession number(s): O77333
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2004
Last sequence update: October 1, 2000
Last modified: June 8, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.