Q9NJH0 (EF1G_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Elongation factor 1-gamma
Short name=EF-1-gamma

Alternative name(s):
eEF-1B gamma

Gene names

Name:	Ef1gamma
Synonyms:	Ef1g
ORF Names:	CG11901

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	431 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Probably plays a role in anchoring the complex to other cellular components.
Subunit structure	EF-1 is composed of four subunits: alpha, beta, delta, and gamma.
Sequence similarities	Contains 1 EF-1-gamma C-terminal domain. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.
Sequence caution	The sequence AAN71367.1 differs from that shown. Reason: Erroneous initiation. The sequence AAS93749.1 differs from that shown. Reason: Erroneous termination at position 322. Translated as Lys.

Ontologies

Keywords
Biological process	Protein biosynthesis
Molecular function	Elongation factor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	salivary gland cell autophagic cell death Inferred from expression pattern. Source: FlyBase
Cellular component	eukaryotic translation elongation factor 1 complex Inferred from electronic annotation. Source: InterPro microtubule associated complex Inferred from direct assay. Source: FlyBase nucleus Inferred from direct assay. Source: FlyBase
Molecular function	translation elongation factor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 431	431	Elongation factor 1-gamma		PRO_0000208824
Regions
	Domain	2 – 84	83	GST N-terminal
	Domain	86 – 212	127	GST C-terminal
	Domain	272 – 431	160	EF-1-gamma C-terminal
Experimental info
	Sequence conflict	112	1	V → D in AAF26671. Ref.1
	Sequence conflict	311	1	A → T in AAS93749. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q9NJH0 [UniParc]. Last modified April 27, 2001. Version 2. Checksum: 9303BACAA3E160D1	FASTA	431	48,968
	10 20 30 40 50 60 MVKGTLYTYP ENFRAYKALI AAQYSGAQVK VADNFKFGET NKSAEFLKKF PGGKVPAFET 70 80 90 100 110 120 AEGQYLSESN AIAYLLANEQ LRGGKCPFVQ AQVQQWISFA DNEIVPASCA WVFPLLGILP 130 140 150 160 170 180 QQKNSTAKQE AEAVLQQLNQ KLQDATFLAG ERITLADIVV FSSLLHLYEY VLEPSVRSAF 190 200 210 220 230 240 GNVNRWFVTI LNQKQVQAVV KDYKLCEKAL VFDPKKYAEF QAKTGAAKPQ QQAQQQKQEK 250 260 270 280 290 300 KPKEKKEAPK KAAEPAEELD AADEALAAEP KSKDPFDALP KGTFNFDDFK RVYSNEDEAK 310 320 330 340 350 360 SIPYFFDKFD AENYSIWFGE YKYNEELSKV FMSCNLITGM FQRLDKMRKA AFASVCLFGE 370 380 390 400 410 420 DGNSTISGIW VWRGQDLAFT LSPDWQIDYE VYDWKKLDAK SEETKKLVTQ YFSWSGTDKD 430 GRKFNQGKIF K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the Drosophila translational elongation factor 1 gamma (EF1g)." Chaney L.C., Hitte C., Kinzy T., Horn M., Rabinow L. Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Strain: Canton-S.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
[5]	Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Rubin G.M., Celniker S.E. Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF148813 mRNA. Translation: AAF26670.1. AF148814 Genomic DNA. Translation: AAF26671.1. AE014297 Genomic DNA. Translation: AAF56877.2. AE014297 Genomic DNA. Translation: AAN14165.1. BT001612 mRNA. Translation: AAN71367.1. Different initiation. BT012478 mRNA. Translation: AAS93749.1. Sequence problems.
RefSeq	NP_001189308.1. NM_001202379.1. NP_652000.1. NM_143743.2. NP_733280.1. NM_170401.2.
3D structure databases
ProteinModelPortal	Q9NJH0.
SMR	Q9NJH0. Positions 1-211, 272-431.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-18800N.
IntAct	Q9NJH0. 2 interactions.
MINT	MINT-879235.
STRING	Q9NJH0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0085392; FBpp0084761; FBgn0029176. FBtr0085393; FBpp0084762; FBgn0029176. FBtr0302442; FBpp0291632; FBgn0029176.
GeneID	44791.
KEGG	dme:Dmel_CG11901.
UCSC	CG11901-RA. d. melanogaster.
Organism-specific databases
CTD	44791.
FlyBase	FBgn0029176. Ef1gamma.
Phylogenomic databases
eggNOG	inNOG07112.
GeneTree	EMGT00050000017845.
InParanoid	Q9NJH0.
OMA	DISVACN.
OrthoDB	EOG4M906Z.
PhylomeDB	Q9NJH0.
Gene expression databases
Bgee	Q9NJH0.
GermOnline	CG11901. Drosophila melanogaster.
Family and domain databases
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR004046. GST_C. IPR012336. Thioredoxin-like_fold. IPR001662. Transl_elong_EF1_G_con. [Graphical view]
Gene3D	G3DSA:1.20.1050.10. GST_C_like. 1 hit. G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit. G3DSA:3.30.70.1010. Transl_elong_EF1_G_con. 1 hit.
KO	K03233.
Pfam	PF00647. EF1G. 1 hit. PF00043. GST_C. 1 hit. PF02798. GST_N. 1 hit. [Graphical view]
SUPFAM	SSF47616. GST_C_like. 1 hit. SSF52833. Thiordxn-like_fd. 1 hit. SSF89942. Transl_elong_EF1_G_con. 1 hit.
PROSITE	PS50040. EF1G_C. 1 hit. PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	837636.

Entry information

Entry name

EF1G_DROME

Accession

Primary (citable) accession number: Q9NJH0
Secondary accession number(s): A4V3K9 , Q6NL63, Q8IGT6, Q9NJH1, Q9VAM7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	April 27, 2001
Last modified:	January 25, 2012
This is version 91 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project