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Q9NJG9

- SUZ12_DROME

UniProt

Q9NJG9 - SUZ12_DROME

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Protein

Polycomb protein Su(z)12

Gene

Su(z)12

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Polycomb group (PcG) protein. While PcG proteins are generally required to maintain the transcriptionally repressive state of homeotic genes throughout development, this protein is specifically required during the first 6 hours of embryogenesis to establish the repressed state. Component of the Esc/E(z) complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. The Esc/E(z) complex is necessary but not sufficient for the repression of homeotic target genes, suggesting that the recruitment of the distinct PRC1 complex is also required.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri411 – 43424C2H2-typeAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: UniProtKB
  2. histone methyltransferase activity Source: FlyBase
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. dendrite morphogenesis Source: FlyBase
  2. gene silencing Source: UniProtKB
  3. histone H3-K27 methylation Source: GOC
  4. histone methylation Source: FlyBase
  5. muscle organ development Source: FlyBase
  6. negative regulation of response to gamma radiation Source: FlyBase
  7. negative regulation of transcription, DNA-templated Source: UniProtKB
  8. neurogenesis Source: FlyBase
  9. neuron development Source: FlyBase
  10. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Developmental protein, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_181712. Oxidative Stress Induced Senescence.
REACT_271477. PRC2 methylates histones and DNA.

Names & Taxonomyi

Protein namesi
Recommended name:
Polycomb protein Su(z)12
Alternative name(s):
Suppressor 12 of zeste protein
Gene namesi
Name:Su(z)12
ORF Names:CG8013
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0020887. Su(z)12.

Subcellular locationi

GO - Cellular componenti

  1. ESC/E(Z) complex Source: FlyBase
  2. histone methyltransferase complex Source: FlyBase
  3. nuclear chromatin Source: UniProtKB
  4. nucleus Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi274 – 2741G → D in Su(z)12-2; induces larval lethality; when homozygous.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 900900Polycomb protein Su(z)12PRO_0000047059Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei547 – 5471Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9NJG9.
PRIDEiQ9NJG9.

Expressioni

Gene expression databases

BgeeiQ9NJG9.

Interactioni

Subunit structurei

Component of the Esc/E(z) complex, composed of Caf1, esc, E(z), Su(z)12, and possibly pho. The Esc/E(z) complex may also associate with Pcl and Rpd3 during early embryogenesis. This complex is distinct from the PRC1 complex, which contains many other PcG proteins like Pc, Ph, Psc, Su(z)2. The two complexes however cooperate and interact together during the first 3 hours of development to establish PcG silencing. Interacts with corto in vitro.3 Publications

Protein-protein interaction databases

BioGridi71118. 4 interactions.
IntActiQ9NJG9. 5 interactions.
MINTiMINT-5227189.
STRINGi7227.FBpp0074686.

Structurei

Secondary structure

1
900
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi84 – 863Combined sources
Helixi88 – 903Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2YB8X-ray2.30A79-91[»]
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9NJG9.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni527 – 60377VEFS-boxAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi355 – 36612Asn-richAdd
BLAST
Compositional biasi699 – 879181Ser-richAdd
BLAST

Sequence similaritiesi

Contains 1 C2H2-type zinc finger.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri411 – 43424C2H2-typeAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiNOG313866.
GeneTreeiENSGT00390000012364.
InParanoidiQ9NJG9.
KOiK11463.
OMAiADNQMNE.
OrthoDBiEOG7DC23W.
PhylomeDBiQ9NJG9.

Family and domain databases

InterProiIPR019135. Polycomb_protein_VEFS-Box.
IPR015880. Znf_C2H2-like.
[Graphical view]
PfamiPF09733. VEFS-Box. 1 hit.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 1 hit.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9NJG9-1) [UniParc]FASTAAdd to Basket

Also known as: B

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAPAKKREKD SNPDGSAANG IIGLTHGAPD ASNAGSTVPP TAEGQVKLNG
60 70 80 90 100
HQQEQELFLQ AFEKPTQIYR YLRNRHETNP IFLNRTLSYM KERMSRNNKK
110 120 130 140 150
RISFQVNSML ESITQKSEAV SQNYLHVIYD SLHEKLPARL DNESGEDLLQ
160 170 180 190 200
EQLLCEAGES VSVETTLYKI TRSKRKDSTL DFQELLSKCS QIVYNPKDRV
210 220 230 240 250
GEHATISIPL QTMRPMGEQH TLYKLLFRIK VLSPSTCNDE NAETPPNKRS
260 270 280 290 300
RPNEKMFGSE LILYEKSSGF ITEGEYEAML QPLNSTSIKS FSPKKCTWET
310 320 330 340 350
MPDSYIPLSL TYDVYQQSPM LKFHLTLSNE QLPEMISAPE LQRYVQHLDA
360 370 380 390 400
VAEMNYNNNN YNNNNNCSGL KNGSGGGNST VCKTTPEHIQ IVYNFMYSNN
410 420 430 440 450
TRQQTEYTQE LNCPWCGLDC LRLYALLKHL KLCHARFNFT YQPAGSGARI
460 470 480 490 500
DVTINDAYDG SYAGSPYDLA GPSGSSFART CGPVRRTSVT SLMVCRPRRQ
510 520 530 540 550
KTCLDEFLEL DEDEISNQRS YITGHNRLYH HTETCLPVHP KELDIDSEGE
560 570 580 590 600
SDPLWLRQKT IQMIDEFSDV NEGEKELMKL WNLHVMRHGF VGDCQLPIAC
610 620 630 640 650
EMFLDAKGTE IVRKNLYRNF ILHMCSLFDY GLIAAETVYK TVQKLQGLLS
660 670 680 690 700
KYAAGQELMQ RQREEQLKYW LDVGMHKKQE DPKTLKSPQK PAPPADQAST
710 720 730 740 750
SSASTSGSGS GSSSMQPPKR MPAHLKRGSA ASSPGVQSKG TENGTNGSNS
760 770 780 790 800
SSSNSKNVAK KSADQPLSTL ANTRERRSEY GQKRNVSGSR LAATPASKRK
810 820 830 840 850
LSSKDNTVLN KRQRYSDGSP GTGIGNGHGG GSGSGANRNK SNNHSLPATS
860 870 880 890 900
NNASSSSSNS KRAIARRRST SERTKASGST GGGAGGVRTR LSVPAKYERR
Length:900
Mass (Da):100,104
Last modified:October 1, 2000 - v1
Checksum:i53BA0D83C49EC92F
GO
Isoform 2 (identifier: Q9NJG9-2) [UniParc]FASTAAdd to Basket

Also known as: A

The sequence of this isoform differs from the canonical sequence as follows:
     806-855: NTVLNKRQRY...LPATSNNASS → VEQAADAPEV...SPVANNCVGN
     856-900: Missing.

Note: No experimental confirmation available.

Show »
Length:855
Mass (Da):95,316
Checksum:iC10FFC6013954105
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei806 – 85550NTVLN…NNASS → VEQAADAPEVLTHSDNAVDV GIIDDECGGFGAVGVMNGVA SPVANNCVGN in isoform 2. 1 PublicationVSP_007033Add
BLAST
Alternative sequencei856 – 90045Missing in isoform 2. 1 PublicationVSP_007034Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF149047 mRNA. Translation: AAF73149.1.
AE014296 Genomic DNA. Translation: AAF49094.2.
AE014296 Genomic DNA. Translation: AAN11641.1.
AY069809 mRNA. Translation: AAL39954.1.
RefSeqiNP_652059.1. NM_143802.3. [Q9NJG9-2]
NP_730465.1. NM_168826.2. [Q9NJG9-1]
UniGeneiDm.1941.

Genome annotation databases

EnsemblMetazoaiFBtr0074917; FBpp0074686; FBgn0020887. [Q9NJG9-1]
GeneIDi48071.
KEGGidme:Dmel_CG8013.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF149047 mRNA. Translation: AAF73149.1 .
AE014296 Genomic DNA. Translation: AAF49094.2 .
AE014296 Genomic DNA. Translation: AAN11641.1 .
AY069809 mRNA. Translation: AAL39954.1 .
RefSeqi NP_652059.1. NM_143802.3. [Q9NJG9-2 ]
NP_730465.1. NM_168826.2. [Q9NJG9-1 ]
UniGenei Dm.1941.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2YB8 X-ray 2.30 A 79-91 [» ]
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 71118. 4 interactions.
IntActi Q9NJG9. 5 interactions.
MINTi MINT-5227189.
STRINGi 7227.FBpp0074686.

Proteomic databases

PaxDbi Q9NJG9.
PRIDEi Q9NJG9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0074917 ; FBpp0074686 ; FBgn0020887 . [Q9NJG9-1 ]
GeneIDi 48071.
KEGGi dme:Dmel_CG8013.

Organism-specific databases

CTDi 48071.
FlyBasei FBgn0020887. Su(z)12.

Phylogenomic databases

eggNOGi NOG313866.
GeneTreei ENSGT00390000012364.
InParanoidi Q9NJG9.
KOi K11463.
OMAi ADNQMNE.
OrthoDBi EOG7DC23W.
PhylomeDBi Q9NJG9.

Enzyme and pathway databases

Reactomei REACT_181712. Oxidative Stress Induced Senescence.
REACT_271477. PRC2 methylates histones and DNA.

Miscellaneous databases

EvolutionaryTracei Q9NJG9.
GenomeRNAii 48071.
NextBioi 839264.
PROi Q9NJG9.

Gene expression databases

Bgeei Q9NJG9.

Family and domain databases

InterProi IPR019135. Polycomb_protein_VEFS-Box.
IPR015880. Znf_C2H2-like.
[Graphical view ]
Pfami PF09733. VEFS-Box. 1 hit.
[Graphical view ]
SMARTi SM00355. ZnF_C2H2. 1 hit.
[Graphical view ]
PROSITEi PS00028. ZINC_FINGER_C2H2_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Su(z)12, a novel Drosophila Polycomb group gene that is conserved in vertebrates and plants."
    Birve A., Sengupta A.K., Beuchle D., Larsson J., Kennison J.A., Rasmuson-Lestander A., Mueller J.
    Development 128:3371-3379(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE (ISOFORM 1), CHARACTERIZATION, MUTANT SU(Z)12-2.
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
    Strain: Berkeley.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: Berkeley.
    Tissue: Embryo.
  5. "Drosophila Enhancer of zeste/ESC complexes have a histone H3 methyltransferase activity that marks chromosomal Polycomb sites."
    Czermin B., Melfi R., McCabe D., Seitz V., Imhof A., Pirrotta V.
    Cell 111:185-196(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN AN ESC/E(Z) COMPLEX WITH CAF1; ESC; E(Z) AND RPD3, METHYLTRANSFERASE ACTIVITY OF THE COMPLEX.
  6. "Histone methyltransferase activity of a Drosophila Polycomb group repressor complex."
    Mueller J., Hart C.M., Francis N.J., Vargas M.L., Sengupta A., Wild B., Miller E.L., O'Connor M.B., Kingston R.E., Simon J.A.
    Cell 111:197-208(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN AN ESC/E(Z) COMPLEX WITH CAF1; ESC AND E(Z), METHYLTRANSFERASE ACTIVITY OF THE COMPLEX.
  7. "A 1-megadalton ESC/E(Z) complex from Drosophila that contains polycomblike and RPD3."
    Tie F., Prasad-Sinha J., Birve A., Rasmuson-Lestander A., Harte P.J.
    Mol. Cell. Biol. 23:3352-3362(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN AN ESC/E(Z) COMPLEX WITH CAF1; ESC; E(Z); PCL AND RPD3.
  8. "An integrated chemical, mass spectrometric and computational strategy for (quantitative) phosphoproteomics: application to Drosophila melanogaster Kc167 cells."
    Bodenmiller B., Mueller L.N., Pedrioli P.G.A., Pflieger D., Juenger M.A., Eng J.K., Aebersold R., Tao W.A.
    Mol. Biosyst. 3:275-286(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-547, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiSUZ12_DROME
AccessioniPrimary (citable) accession number: Q9NJG9
Secondary accession number(s): Q8T9D8, Q9VW55
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 28, 2003
Last sequence update: October 1, 2000
Last modified: November 26, 2014
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3