Q9NJG9 (SUZ12_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Polycomb protein Su(z)12

Alternative name(s):
Suppressor 12 of zeste protein

Gene names

Name:	Su(z)12
ORF Names:	CG8013

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	900 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Polycomb group (PcG) protein. While PcG proteins are generally required to maintain the transcriptionally repressive state of homeotic genes throughout development, this protein is specifically required during the first 6 hours of embryogenesis to establish the repressed state. Component of the Esc/E(z) complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. The Esc/E(z) complex is necessary but not sufficient for the repression of homeotic target genes, suggesting that the recruitment of the distinct PRC1 complex is also required.
Subunit structure	Component of the Esc/E(z) complex, composed of Caf1, esc, E(z), Su(z)12, and possibly pho. The Esc/E(z) complex may also associate with Pcl and Rpd3 during early embryogenesis. This complex is distinct from the PRC1 complex, which contains many other PcG proteins like Pc, Ph, Psc, Su(z)2. The two complexes however cooperate and interact together during the first 3 hours of development to establish PcG silencing. Interacts with corto in vitro. Ref.5 Ref.6 Ref.7
Subcellular location	Nucleus.
Sequence similarities	Belongs to the VEFS (VRN2-EMF2-FIS2-SU(Z)12) family. Contains 1 C2H2-type zinc finger.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Chromatin regulator Developmental protein Repressor
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	dendrite morphogenesis Inferred from mutant phenotype PubMed 16547170. Source: FlyBase gene silencing Non-traceable author statement Ref.5. Source: UniProtKB histone H3-K27 methylation Inferred by curator Ref.6. Source: GOC muscle organ development Inferred from mutant phenotype PubMed 16547170. Source: FlyBase negative regulation of response to gamma radiation Inferred from mutant phenotype PubMed 18410726. Source: FlyBase negative regulation of transcription, DNA-dependent Non-traceable author statement Ref.5. Source: UniProtKB transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	ESC/E(Z) complex Inferred from direct assay Ref.5Ref.6PubMed 18276122PubMed 22354997. Source: FlyBase nuclear chromatin Non-traceable author statement Ref.5. Source: UniProtKB
Molecular_function	DNA binding Non-traceable author statement Ref.5. Source: UniProtKB histone methyltransferase activity Inferred from direct assay PubMed 16055700. Source: FlyBase zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9NJG9-1)

Also known as: B;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9NJG9-2)

Also known as: A;

The sequence of this isoform differs from the canonical sequence as follows:
     806-855: NTVLNKRQRY...LPATSNNASS → VEQAADAPEV...SPVANNCVGN
     856-900: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 900

900

Polycomb protein Su(z)12

PRO_0000047059

Regions

Zinc finger

411 – 434

24

C2H2-type

Region

527 – 603

77

VEFS-box

Compositional bias

355 – 366

12

Asn-rich

Compositional bias

699 – 879

181

Ser-rich

Amino acid modifications

Modified residue

547

1

Phosphoserine Ref.8

Natural variations

Alternative sequence

806 – 855

50

NTVLN…NNASS → VEQAADAPEVLTHSDNAVDV GIIDDECGGFGAVGVMNGVA SPVANNCVGN in isoform 2.

VSP_007033

Alternative sequence

856 – 900

45

Missing in isoform 2.

VSP_007034

Experimental info

Mutagenesis

274

1

G → D in Su(z)12-2; induces larval lethality; when homozygous.

Secondary structure

1

.

.

.

.

.

900

Helix Strand Turn

Details...

Helix

84 – 86

3

Helix

88 – 90

3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (B) [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 53BA0D83C49EC92F	FASTA	900	100,104
	10 20 30 40 50 60 MAPAKKREKD SNPDGSAANG IIGLTHGAPD ASNAGSTVPP TAEGQVKLNG HQQEQELFLQ 70 80 90 100 110 120 AFEKPTQIYR YLRNRHETNP IFLNRTLSYM KERMSRNNKK RISFQVNSML ESITQKSEAV 130 140 150 160 170 180 SQNYLHVIYD SLHEKLPARL DNESGEDLLQ EQLLCEAGES VSVETTLYKI TRSKRKDSTL 190 200 210 220 230 240 DFQELLSKCS QIVYNPKDRV GEHATISIPL QTMRPMGEQH TLYKLLFRIK VLSPSTCNDE 250 260 270 280 290 300 NAETPPNKRS RPNEKMFGSE LILYEKSSGF ITEGEYEAML QPLNSTSIKS FSPKKCTWET 310 320 330 340 350 360 MPDSYIPLSL TYDVYQQSPM LKFHLTLSNE QLPEMISAPE LQRYVQHLDA VAEMNYNNNN 370 380 390 400 410 420 YNNNNNCSGL KNGSGGGNST VCKTTPEHIQ IVYNFMYSNN TRQQTEYTQE LNCPWCGLDC 430 440 450 460 470 480 LRLYALLKHL KLCHARFNFT YQPAGSGARI DVTINDAYDG SYAGSPYDLA GPSGSSFART 490 500 510 520 530 540 CGPVRRTSVT SLMVCRPRRQ KTCLDEFLEL DEDEISNQRS YITGHNRLYH HTETCLPVHP 550 560 570 580 590 600 KELDIDSEGE SDPLWLRQKT IQMIDEFSDV NEGEKELMKL WNLHVMRHGF VGDCQLPIAC 610 620 630 640 650 660 EMFLDAKGTE IVRKNLYRNF ILHMCSLFDY GLIAAETVYK TVQKLQGLLS KYAAGQELMQ 670 680 690 700 710 720 RQREEQLKYW LDVGMHKKQE DPKTLKSPQK PAPPADQAST SSASTSGSGS GSSSMQPPKR 730 740 750 760 770 780 MPAHLKRGSA ASSPGVQSKG TENGTNGSNS SSSNSKNVAK KSADQPLSTL ANTRERRSEY 790 800 810 820 830 840 GQKRNVSGSR LAATPASKRK LSSKDNTVLN KRQRYSDGSP GTGIGNGHGG GSGSGANRNK 850 860 870 880 890 900 SNNHSLPATS NNASSSSSNS KRAIARRRST SERTKASGST GGGAGGVRTR LSVPAKYERR
	Isoform 2 (A) [UniParc]. Checksum: C10FFC6013954105	FASTA	855	95,316
	10 20 30 40 50 60 MAPAKKREKD SNPDGSAANG IIGLTHGAPD ASNAGSTVPP TAEGQVKLNG HQQEQELFLQ 70 80 90 100 110 120 AFEKPTQIYR YLRNRHETNP IFLNRTLSYM KERMSRNNKK RISFQVNSML ESITQKSEAV 130 140 150 160 170 180 SQNYLHVIYD SLHEKLPARL DNESGEDLLQ EQLLCEAGES VSVETTLYKI TRSKRKDSTL 190 200 210 220 230 240 DFQELLSKCS QIVYNPKDRV GEHATISIPL QTMRPMGEQH TLYKLLFRIK VLSPSTCNDE 250 260 270 280 290 300 NAETPPNKRS RPNEKMFGSE LILYEKSSGF ITEGEYEAML QPLNSTSIKS FSPKKCTWET 310 320 330 340 350 360 MPDSYIPLSL TYDVYQQSPM LKFHLTLSNE QLPEMISAPE LQRYVQHLDA VAEMNYNNNN 370 380 390 400 410 420 YNNNNNCSGL KNGSGGGNST VCKTTPEHIQ IVYNFMYSNN TRQQTEYTQE LNCPWCGLDC 430 440 450 460 470 480 LRLYALLKHL KLCHARFNFT YQPAGSGARI DVTINDAYDG SYAGSPYDLA GPSGSSFART 490 500 510 520 530 540 CGPVRRTSVT SLMVCRPRRQ KTCLDEFLEL DEDEISNQRS YITGHNRLYH HTETCLPVHP 550 560 570 580 590 600 KELDIDSEGE SDPLWLRQKT IQMIDEFSDV NEGEKELMKL WNLHVMRHGF VGDCQLPIAC 610 620 630 640 650 660 EMFLDAKGTE IVRKNLYRNF ILHMCSLFDY GLIAAETVYK TVQKLQGLLS KYAAGQELMQ 670 680 690 700 710 720 RQREEQLKYW LDVGMHKKQE DPKTLKSPQK PAPPADQAST SSASTSGSGS GSSSMQPPKR 730 740 750 760 770 780 MPAHLKRGSA ASSPGVQSKG TENGTNGSNS SSSNSKNVAK KSADQPLSTL ANTRERRSEY 790 800 810 820 830 840 GQKRNVSGSR LAATPASKRK LSSKDVEQAA DAPEVLTHSD NAVDVGIIDD ECGGFGAVGV 850 MNGVASPVAN NCVGN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Su(z)12, a novel Drosophila Polycomb group gene that is conserved in vertebrates and plants." Birve A., Sengupta A.K., Beuchle D., Larsson J., Kennison J.A., Rasmuson-Lestander A., Mueller J. Development 128:3371-3379(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE (ISOFORM 1), CHARACTERIZATION, MUTANT SU(Z)12-2.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING. Strain: Berkeley.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: Berkeley. Tissue: Embryo.
[5]	"Drosophila Enhancer of zeste/ESC complexes have a histone H3 methyltransferase activity that marks chromosomal Polycomb sites." Czermin B., Melfi R., McCabe D., Seitz V., Imhof A., Pirrotta V. Cell 111:185-196(2002) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN AN ESC/E(Z) COMPLEX WITH CAF1; ESC; E(Z) AND RPD3, METHYLTRANSFERASE ACTIVITY OF THE COMPLEX.
[6]	"Histone methyltransferase activity of a Drosophila Polycomb group repressor complex." Mueller J., Hart C.M., Francis N.J., Vargas M.L., Sengupta A., Wild B., Miller E.L., O'Connor M.B., Kingston R.E., Simon J.A. Cell 111:197-208(2002) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN AN ESC/E(Z) COMPLEX WITH CAF1; ESC AND E(Z), METHYLTRANSFERASE ACTIVITY OF THE COMPLEX.
[7]	"A 1-megadalton ESC/E(Z) complex from Drosophila that contains polycomblike and RPD3." Tie F., Prasad-Sinha J., Birve A., Rasmuson-Lestander A., Harte P.J. Mol. Cell. Biol. 23:3352-3362(2003) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN AN ESC/E(Z) COMPLEX WITH CAF1; ESC; E(Z); PCL AND RPD3.
[8]	"An integrated chemical, mass spectrometric and computational strategy for (quantitative) phosphoproteomics: application to Drosophila melanogaster Kc167 cells." Bodenmiller B., Mueller L.N., Pedrioli P.G.A., Pflieger D., Juenger M.A., Eng J.K., Aebersold R., Tao W.A. Mol. Biosyst. 3:275-286(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-547, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF149047 mRNA. Translation: AAF73149.1.
AE014296 Genomic DNA. Translation: AAF49094.2.
AE014296 Genomic DNA. Translation: AAN11641.1.
AY069809 mRNA. Translation: AAL39954.1.

RefSeq

NP_652059.1. NM_143802.3.
NP_730465.1. NM_168826.2.

UniGene

Dm.1941.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2YB8	X-ray	2.30	A	79-91	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9NJG9. 5 interactions.

STRING

7227.FBpp0074686.

Proteomic databases

PaxDb

Q9NJG9.

PRIDE

Q9NJG9.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblMetazoa

FBtr0074917; FBpp0074686; FBgn0020887.

GeneID

48071.

KEGG

dme:Dmel_CG8013.

Organism-specific databases

CTD

48071.

FlyBase

FBgn0020887. Su(z)12.

Phylogenomic databases

eggNOG

NOG313866.

GeneTree

ENSGT00390000012364.

InParanoid

Q9NJG9.

KO

K11463.

OMA

SHRNSRN.

OrthoDB

EOG4S7H52.

PhylomeDB

Q9NJG9.

Gene expression databases

Bgee

Q9NJG9.

GermOnline

CG8013. Drosophila melanogaster.

Family and domain databases

InterPro

IPR019135. Polycomb_protein_VEFS-Box.
IPR015880. Znf_C2H2-like.
[Graphical view]

Pfam

PF09733. VEFS-Box. 1 hit.
[Graphical view]

SMART

SM00355. ZnF_C2H2. 1 hit.
[Graphical view]

PROSITE

PS00028. ZINC_FINGER_C2H2_1. 1 hit.
PS50157. ZINC_FINGER_C2H2_2. False negative.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

Su(z)12. drosophila.

EvolutionaryTrace

Q9NJG9.

GenomeRNAi

48071.

NextBio

839264.

Entry information

Entry name

SUZ12_DROME

Accession

Primary (citable) accession number: Q9NJG9
Secondary accession number(s): Q8T9D8, Q9VW55

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 28, 2003
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 99 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project