Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q9NJG9

- SUZ12_DROME

UniProt

Q9NJG9 - SUZ12_DROME

Protein

Polycomb protein Su(z)12

Gene

Su(z)12

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Polycomb group (PcG) protein. While PcG proteins are generally required to maintain the transcriptionally repressive state of homeotic genes throughout development, this protein is specifically required during the first 6 hours of embryogenesis to establish the repressed state. Component of the Esc/E(z) complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. The Esc/E(z) complex is necessary but not sufficient for the repression of homeotic target genes, suggesting that the recruitment of the distinct PRC1 complex is also required.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri411 – 43424C2H2-typeAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB
    2. histone methyltransferase activity Source: FlyBase
    3. metal ion binding Source: UniProtKB-KW
    4. protein binding Source: UniProtKB

    GO - Biological processi

    1. dendrite morphogenesis Source: FlyBase
    2. gene silencing Source: UniProtKB
    3. histone H3-K27 methylation Source: GOC
    4. histone methylation Source: FlyBase
    5. muscle organ development Source: FlyBase
    6. negative regulation of response to gamma radiation Source: FlyBase
    7. negative regulation of transcription, DNA-templated Source: UniProtKB
    8. neurogenesis Source: FlyBase
    9. neuron development Source: FlyBase
    10. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Chromatin regulator, Developmental protein, Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Polycomb protein Su(z)12
    Alternative name(s):
    Suppressor 12 of zeste protein
    Gene namesi
    Name:Su(z)12
    ORF Names:CG8013
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome 3L

    Organism-specific databases

    FlyBaseiFBgn0020887. Su(z)12.

    Subcellular locationi

    GO - Cellular componenti

    1. ESC/E(Z) complex Source: FlyBase
    2. histone methyltransferase complex Source: FlyBase
    3. nuclear chromatin Source: UniProtKB
    4. nucleus Source: FlyBase

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi274 – 2741G → D in Su(z)12-2; induces larval lethality; when homozygous.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 900900Polycomb protein Su(z)12PRO_0000047059Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei547 – 5471Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ9NJG9.
    PRIDEiQ9NJG9.

    Expressioni

    Gene expression databases

    BgeeiQ9NJG9.

    Interactioni

    Subunit structurei

    Component of the Esc/E(z) complex, composed of Caf1, esc, E(z), Su(z)12, and possibly pho. The Esc/E(z) complex may also associate with Pcl and Rpd3 during early embryogenesis. This complex is distinct from the PRC1 complex, which contains many other PcG proteins like Pc, Ph, Psc, Su(z)2. The two complexes however cooperate and interact together during the first 3 hours of development to establish PcG silencing. Interacts with corto in vitro.3 Publications

    Protein-protein interaction databases

    BioGridi71118. 4 interactions.
    IntActiQ9NJG9. 5 interactions.
    MINTiMINT-5227189.
    STRINGi7227.FBpp0074686.

    Structurei

    Secondary structure

    1
    900
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi84 – 863
    Helixi88 – 903

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2YB8X-ray2.30A79-91[»]
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9NJG9.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni527 – 60377VEFS-boxAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi355 – 36612Asn-richAdd
    BLAST
    Compositional biasi699 – 879181Ser-richAdd
    BLAST

    Sequence similaritiesi

    Contains 1 C2H2-type zinc finger.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri411 – 43424C2H2-typeAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiNOG313866.
    GeneTreeiENSGT00390000012364.
    InParanoidiQ9NJG9.
    KOiK11463.
    OMAiADNQMNE.
    OrthoDBiEOG7DC23W.
    PhylomeDBiQ9NJG9.

    Family and domain databases

    InterProiIPR019135. Polycomb_protein_VEFS-Box.
    IPR015880. Znf_C2H2-like.
    [Graphical view]
    PfamiPF09733. VEFS-Box. 1 hit.
    [Graphical view]
    SMARTiSM00355. ZnF_C2H2. 1 hit.
    [Graphical view]
    PROSITEiPS00028. ZINC_FINGER_C2H2_1. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9NJG9-1) [UniParc]FASTAAdd to Basket

    Also known as: B

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAPAKKREKD SNPDGSAANG IIGLTHGAPD ASNAGSTVPP TAEGQVKLNG    50
    HQQEQELFLQ AFEKPTQIYR YLRNRHETNP IFLNRTLSYM KERMSRNNKK 100
    RISFQVNSML ESITQKSEAV SQNYLHVIYD SLHEKLPARL DNESGEDLLQ 150
    EQLLCEAGES VSVETTLYKI TRSKRKDSTL DFQELLSKCS QIVYNPKDRV 200
    GEHATISIPL QTMRPMGEQH TLYKLLFRIK VLSPSTCNDE NAETPPNKRS 250
    RPNEKMFGSE LILYEKSSGF ITEGEYEAML QPLNSTSIKS FSPKKCTWET 300
    MPDSYIPLSL TYDVYQQSPM LKFHLTLSNE QLPEMISAPE LQRYVQHLDA 350
    VAEMNYNNNN YNNNNNCSGL KNGSGGGNST VCKTTPEHIQ IVYNFMYSNN 400
    TRQQTEYTQE LNCPWCGLDC LRLYALLKHL KLCHARFNFT YQPAGSGARI 450
    DVTINDAYDG SYAGSPYDLA GPSGSSFART CGPVRRTSVT SLMVCRPRRQ 500
    KTCLDEFLEL DEDEISNQRS YITGHNRLYH HTETCLPVHP KELDIDSEGE 550
    SDPLWLRQKT IQMIDEFSDV NEGEKELMKL WNLHVMRHGF VGDCQLPIAC 600
    EMFLDAKGTE IVRKNLYRNF ILHMCSLFDY GLIAAETVYK TVQKLQGLLS 650
    KYAAGQELMQ RQREEQLKYW LDVGMHKKQE DPKTLKSPQK PAPPADQAST 700
    SSASTSGSGS GSSSMQPPKR MPAHLKRGSA ASSPGVQSKG TENGTNGSNS 750
    SSSNSKNVAK KSADQPLSTL ANTRERRSEY GQKRNVSGSR LAATPASKRK 800
    LSSKDNTVLN KRQRYSDGSP GTGIGNGHGG GSGSGANRNK SNNHSLPATS 850
    NNASSSSSNS KRAIARRRST SERTKASGST GGGAGGVRTR LSVPAKYERR 900
    Length:900
    Mass (Da):100,104
    Last modified:October 1, 2000 - v1
    Checksum:i53BA0D83C49EC92F
    GO
    Isoform 2 (identifier: Q9NJG9-2) [UniParc]FASTAAdd to Basket

    Also known as: A

    The sequence of this isoform differs from the canonical sequence as follows:
         806-855: NTVLNKRQRY...LPATSNNASS → VEQAADAPEV...SPVANNCVGN
         856-900: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:855
    Mass (Da):95,316
    Checksum:iC10FFC6013954105
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei806 – 85550NTVLN…NNASS → VEQAADAPEVLTHSDNAVDV GIIDDECGGFGAVGVMNGVA SPVANNCVGN in isoform 2. 1 PublicationVSP_007033Add
    BLAST
    Alternative sequencei856 – 90045Missing in isoform 2. 1 PublicationVSP_007034Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF149047 mRNA. Translation: AAF73149.1.
    AE014296 Genomic DNA. Translation: AAF49094.2.
    AE014296 Genomic DNA. Translation: AAN11641.1.
    AY069809 mRNA. Translation: AAL39954.1.
    RefSeqiNP_652059.1. NM_143802.3. [Q9NJG9-2]
    NP_730465.1. NM_168826.2. [Q9NJG9-1]
    UniGeneiDm.1941.

    Genome annotation databases

    EnsemblMetazoaiFBtr0074917; FBpp0074686; FBgn0020887. [Q9NJG9-1]
    GeneIDi48071.
    KEGGidme:Dmel_CG8013.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF149047 mRNA. Translation: AAF73149.1 .
    AE014296 Genomic DNA. Translation: AAF49094.2 .
    AE014296 Genomic DNA. Translation: AAN11641.1 .
    AY069809 mRNA. Translation: AAL39954.1 .
    RefSeqi NP_652059.1. NM_143802.3. [Q9NJG9-2 ]
    NP_730465.1. NM_168826.2. [Q9NJG9-1 ]
    UniGenei Dm.1941.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2YB8 X-ray 2.30 A 79-91 [» ]
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 71118. 4 interactions.
    IntActi Q9NJG9. 5 interactions.
    MINTi MINT-5227189.
    STRINGi 7227.FBpp0074686.

    Proteomic databases

    PaxDbi Q9NJG9.
    PRIDEi Q9NJG9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0074917 ; FBpp0074686 ; FBgn0020887 . [Q9NJG9-1 ]
    GeneIDi 48071.
    KEGGi dme:Dmel_CG8013.

    Organism-specific databases

    CTDi 48071.
    FlyBasei FBgn0020887. Su(z)12.

    Phylogenomic databases

    eggNOGi NOG313866.
    GeneTreei ENSGT00390000012364.
    InParanoidi Q9NJG9.
    KOi K11463.
    OMAi ADNQMNE.
    OrthoDBi EOG7DC23W.
    PhylomeDBi Q9NJG9.

    Miscellaneous databases

    ChiTaRSi Su(z)12. drosophila.
    EvolutionaryTracei Q9NJG9.
    GenomeRNAii 48071.
    NextBioi 839264.
    PROi Q9NJG9.

    Gene expression databases

    Bgeei Q9NJG9.

    Family and domain databases

    InterProi IPR019135. Polycomb_protein_VEFS-Box.
    IPR015880. Znf_C2H2-like.
    [Graphical view ]
    Pfami PF09733. VEFS-Box. 1 hit.
    [Graphical view ]
    SMARTi SM00355. ZnF_C2H2. 1 hit.
    [Graphical view ]
    PROSITEi PS00028. ZINC_FINGER_C2H2_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Su(z)12, a novel Drosophila Polycomb group gene that is conserved in vertebrates and plants."
      Birve A., Sengupta A.K., Beuchle D., Larsson J., Kennison J.A., Rasmuson-Lestander A., Mueller J.
      Development 128:3371-3379(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE (ISOFORM 1), CHARACTERIZATION, MUTANT SU(Z)12-2.
    2. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley.
    3. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
      Strain: Berkeley.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: Berkeley.
      Tissue: Embryo.
    5. "Drosophila Enhancer of zeste/ESC complexes have a histone H3 methyltransferase activity that marks chromosomal Polycomb sites."
      Czermin B., Melfi R., McCabe D., Seitz V., Imhof A., Pirrotta V.
      Cell 111:185-196(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN AN ESC/E(Z) COMPLEX WITH CAF1; ESC; E(Z) AND RPD3, METHYLTRANSFERASE ACTIVITY OF THE COMPLEX.
    6. "Histone methyltransferase activity of a Drosophila Polycomb group repressor complex."
      Mueller J., Hart C.M., Francis N.J., Vargas M.L., Sengupta A., Wild B., Miller E.L., O'Connor M.B., Kingston R.E., Simon J.A.
      Cell 111:197-208(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN AN ESC/E(Z) COMPLEX WITH CAF1; ESC AND E(Z), METHYLTRANSFERASE ACTIVITY OF THE COMPLEX.
    7. "A 1-megadalton ESC/E(Z) complex from Drosophila that contains polycomblike and RPD3."
      Tie F., Prasad-Sinha J., Birve A., Rasmuson-Lestander A., Harte P.J.
      Mol. Cell. Biol. 23:3352-3362(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN AN ESC/E(Z) COMPLEX WITH CAF1; ESC; E(Z); PCL AND RPD3.
    8. "An integrated chemical, mass spectrometric and computational strategy for (quantitative) phosphoproteomics: application to Drosophila melanogaster Kc167 cells."
      Bodenmiller B., Mueller L.N., Pedrioli P.G.A., Pflieger D., Juenger M.A., Eng J.K., Aebersold R., Tao W.A.
      Mol. Biosyst. 3:275-286(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-547, IDENTIFICATION BY MASS SPECTROMETRY.

    Entry informationi

    Entry nameiSUZ12_DROME
    AccessioniPrimary (citable) accession number: Q9NJG9
    Secondary accession number(s): Q8T9D8, Q9VW55
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 28, 2003
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 109 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3