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Q9NJG9 (SUZ12_DROME) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Polycomb protein Su(z)12
Alternative name(s):
Suppressor 12 of zeste protein
Gene names
Name:Su(z)12
ORF Names:CG8013
OrganismDrosophila melanogaster (Fruit fly) [Reference proteome]
Taxonomic identifier7227 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora

Protein attributes

Sequence length900 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Polycomb group (PcG) protein. While PcG proteins are generally required to maintain the transcriptionally repressive state of homeotic genes throughout development, this protein is specifically required during the first 6 hours of embryogenesis to establish the repressed state. Component of the Esc/E(z) complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. The Esc/E(z) complex is necessary but not sufficient for the repression of homeotic target genes, suggesting that the recruitment of the distinct PRC1 complex is also required.

Subunit structure

Component of the Esc/E(z) complex, composed of Caf1, esc, E(z), Su(z)12, and possibly pho. The Esc/E(z) complex may also associate with Pcl and Rpd3 during early embryogenesis. This complex is distinct from the PRC1 complex, which contains many other PcG proteins like Pc, Ph, Psc, Su(z)2. The two complexes however cooperate and interact together during the first 3 hours of development to establish PcG silencing. Interacts with corto in vitro. Ref.5 Ref.6 Ref.7

Subcellular location

Nucleus.

Sequence similarities

Belongs to the VEFS (VRN2-EMF2-FIS2-SU(Z)12) family.

Contains 1 C2H2-type zinc finger.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
   DomainZinc-finger
   LigandMetal-binding
Zinc
   Molecular functionChromatin regulator
Developmental protein
Repressor
   PTMPhosphoprotein
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processdendrite morphogenesis

Inferred from mutant phenotype PubMed 16547170. Source: FlyBase

gene silencing

Non-traceable author statement Ref.5. Source: UniProtKB

histone H3-K27 methylation

Inferred by curator Ref.6. Source: GOC

histone methylation

Inferred from direct assay Ref.6. Source: FlyBase

muscle organ development

Inferred from mutant phenotype PubMed 16547170. Source: FlyBase

negative regulation of response to gamma radiation

Inferred from mutant phenotype PubMed 18410726. Source: FlyBase

negative regulation of transcription, DNA-templated

Non-traceable author statement Ref.5. Source: UniProtKB

neurogenesis

Inferred from mutant phenotype PubMed 17287254PubMed 21549331. Source: FlyBase

neuron development

Inferred from mutant phenotype PubMed 16547170. Source: FlyBase

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentESC/E(Z) complex

Inferred from direct assay Ref.5Ref.6PubMed 18276122PubMed 22354997. Source: FlyBase

histone methyltransferase complex

Inferred from direct assay PubMed 16055700. Source: FlyBase

nuclear chromatin

Non-traceable author statement Ref.5. Source: UniProtKB

nucleus

Inferred from direct assay Ref.5PubMed 16618800. Source: FlyBase

   Molecular_functionDNA binding

Non-traceable author statement Ref.5. Source: UniProtKB

histone methyltransferase activity

Inferred from direct assay PubMed 16055700. Source: FlyBase

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9NJG9-1)

Also known as: B;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9NJG9-2)

Also known as: A;

The sequence of this isoform differs from the canonical sequence as follows:
     806-855: NTVLNKRQRY...LPATSNNASS → VEQAADAPEV...SPVANNCVGN
     856-900: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 900900Polycomb protein Su(z)12
PRO_0000047059

Regions

Zinc finger411 – 43424C2H2-type
Region527 – 60377VEFS-box
Compositional bias355 – 36612Asn-rich
Compositional bias699 – 879181Ser-rich

Amino acid modifications

Modified residue5471Phosphoserine Ref.8

Natural variations

Alternative sequence806 – 85550NTVLN…NNASS → VEQAADAPEVLTHSDNAVDV GIIDDECGGFGAVGVMNGVA SPVANNCVGN in isoform 2.
VSP_007033
Alternative sequence856 – 90045Missing in isoform 2.
VSP_007034

Experimental info

Mutagenesis2741G → D in Su(z)12-2; induces larval lethality; when homozygous.

Secondary structure

..... 900
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (B) [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 53BA0D83C49EC92F

FASTA900100,104
        10         20         30         40         50         60 
MAPAKKREKD SNPDGSAANG IIGLTHGAPD ASNAGSTVPP TAEGQVKLNG HQQEQELFLQ 

        70         80         90        100        110        120 
AFEKPTQIYR YLRNRHETNP IFLNRTLSYM KERMSRNNKK RISFQVNSML ESITQKSEAV 

       130        140        150        160        170        180 
SQNYLHVIYD SLHEKLPARL DNESGEDLLQ EQLLCEAGES VSVETTLYKI TRSKRKDSTL 

       190        200        210        220        230        240 
DFQELLSKCS QIVYNPKDRV GEHATISIPL QTMRPMGEQH TLYKLLFRIK VLSPSTCNDE 

       250        260        270        280        290        300 
NAETPPNKRS RPNEKMFGSE LILYEKSSGF ITEGEYEAML QPLNSTSIKS FSPKKCTWET 

       310        320        330        340        350        360 
MPDSYIPLSL TYDVYQQSPM LKFHLTLSNE QLPEMISAPE LQRYVQHLDA VAEMNYNNNN 

       370        380        390        400        410        420 
YNNNNNCSGL KNGSGGGNST VCKTTPEHIQ IVYNFMYSNN TRQQTEYTQE LNCPWCGLDC 

       430        440        450        460        470        480 
LRLYALLKHL KLCHARFNFT YQPAGSGARI DVTINDAYDG SYAGSPYDLA GPSGSSFART 

       490        500        510        520        530        540 
CGPVRRTSVT SLMVCRPRRQ KTCLDEFLEL DEDEISNQRS YITGHNRLYH HTETCLPVHP 

       550        560        570        580        590        600 
KELDIDSEGE SDPLWLRQKT IQMIDEFSDV NEGEKELMKL WNLHVMRHGF VGDCQLPIAC 

       610        620        630        640        650        660 
EMFLDAKGTE IVRKNLYRNF ILHMCSLFDY GLIAAETVYK TVQKLQGLLS KYAAGQELMQ 

       670        680        690        700        710        720 
RQREEQLKYW LDVGMHKKQE DPKTLKSPQK PAPPADQAST SSASTSGSGS GSSSMQPPKR 

       730        740        750        760        770        780 
MPAHLKRGSA ASSPGVQSKG TENGTNGSNS SSSNSKNVAK KSADQPLSTL ANTRERRSEY 

       790        800        810        820        830        840 
GQKRNVSGSR LAATPASKRK LSSKDNTVLN KRQRYSDGSP GTGIGNGHGG GSGSGANRNK 

       850        860        870        880        890        900 
SNNHSLPATS NNASSSSSNS KRAIARRRST SERTKASGST GGGAGGVRTR LSVPAKYERR 

« Hide

Isoform 2 (A) [UniParc].

Checksum: C10FFC6013954105
Show »

FASTA85595,316

References

« Hide 'large scale' references
[1]"Su(z)12, a novel Drosophila Polycomb group gene that is conserved in vertebrates and plants."
Birve A., Sengupta A.K., Beuchle D., Larsson J., Kennison J.A., Rasmuson-Lestander A., Mueller J.
Development 128:3371-3379(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE (ISOFORM 1), CHARACTERIZATION, MUTANT SU(Z)12-2.
[2]"The genome sequence of Drosophila melanogaster."
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. expand/collapse author list , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Berkeley.
[3]"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review."
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. expand/collapse author list , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
Strain: Berkeley.
[4]"A Drosophila full-length cDNA resource."
Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E.
Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: Berkeley.
Tissue: Embryo.
[5]"Drosophila Enhancer of zeste/ESC complexes have a histone H3 methyltransferase activity that marks chromosomal Polycomb sites."
Czermin B., Melfi R., McCabe D., Seitz V., Imhof A., Pirrotta V.
Cell 111:185-196(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN AN ESC/E(Z) COMPLEX WITH CAF1; ESC; E(Z) AND RPD3, METHYLTRANSFERASE ACTIVITY OF THE COMPLEX.
[6]"Histone methyltransferase activity of a Drosophila Polycomb group repressor complex."
Mueller J., Hart C.M., Francis N.J., Vargas M.L., Sengupta A., Wild B., Miller E.L., O'Connor M.B., Kingston R.E., Simon J.A.
Cell 111:197-208(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN AN ESC/E(Z) COMPLEX WITH CAF1; ESC AND E(Z), METHYLTRANSFERASE ACTIVITY OF THE COMPLEX.
[7]"A 1-megadalton ESC/E(Z) complex from Drosophila that contains polycomblike and RPD3."
Tie F., Prasad-Sinha J., Birve A., Rasmuson-Lestander A., Harte P.J.
Mol. Cell. Biol. 23:3352-3362(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN AN ESC/E(Z) COMPLEX WITH CAF1; ESC; E(Z); PCL AND RPD3.
[8]"An integrated chemical, mass spectrometric and computational strategy for (quantitative) phosphoproteomics: application to Drosophila melanogaster Kc167 cells."
Bodenmiller B., Mueller L.N., Pedrioli P.G.A., Pflieger D., Juenger M.A., Eng J.K., Aebersold R., Tao W.A.
Mol. Biosyst. 3:275-286(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-547, IDENTIFICATION BY MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF149047 mRNA. Translation: AAF73149.1.
AE014296 Genomic DNA. Translation: AAF49094.2.
AE014296 Genomic DNA. Translation: AAN11641.1.
AY069809 mRNA. Translation: AAL39954.1.
RefSeqNP_652059.1. NM_143802.3.
NP_730465.1. NM_168826.2.
UniGeneDm.1941.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2YB8X-ray2.30A79-91[»]
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid71118. 4 interactions.
IntActQ9NJG9. 5 interactions.
MINTMINT-5227189.
STRING7227.FBpp0074686.

Proteomic databases

PaxDbQ9NJG9.
PRIDEQ9NJG9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaFBtr0074917; FBpp0074686; FBgn0020887. [Q9NJG9-1]
GeneID48071.
KEGGdme:Dmel_CG8013.

Organism-specific databases

CTD48071.
FlyBaseFBgn0020887. Su(z)12.

Phylogenomic databases

eggNOGNOG313866.
GeneTreeENSGT00390000012364.
InParanoidQ9NJG9.
KOK11463.
OMAADNQMNE.
OrthoDBEOG7DC23W.
PhylomeDBQ9NJG9.

Gene expression databases

BgeeQ9NJG9.

Family and domain databases

InterProIPR019135. Polycomb_protein_VEFS-Box.
IPR015880. Znf_C2H2-like.
[Graphical view]
PfamPF09733. VEFS-Box. 1 hit.
[Graphical view]
SMARTSM00355. ZnF_C2H2. 1 hit.
[Graphical view]
PROSITEPS00028. ZINC_FINGER_C2H2_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSSu(z)12. drosophila.
EvolutionaryTraceQ9NJG9.
GenomeRNAi48071.
NextBio839264.
PROQ9NJG9.

Entry information

Entry nameSUZ12_DROME
AccessionPrimary (citable) accession number: Q9NJG9
Secondary accession number(s): Q8T9D8, Q9VW55
Entry history
Integrated into UniProtKB/Swiss-Prot: March 28, 2003
Last sequence update: October 1, 2000
Last modified: April 16, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase