Reviewed, UniProtKB/Swiss-Prot Q9NJD8 (GMPR_ONCVO)

Names and origin

Protein names

Recommended name:
    GMP reductase
    EC=1.7.1.7
Alternative name(s):
    Guanosine 5'-monophosphate oxidoreductase
      Short name=Guanosine monophosphate reductase

Gene names

Name:

gmr-1

Organism

Onchocerca volvulus

Taxonomic identifier

6282 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Spirurida › Filarioidea › Onchocercidae › Onchocerca

Protein attributes

Sequence length	364 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the irreversible NADPH-dependent deamination of GMP to IMP. It functions in the conversion of nucleobase, nucleoside and nucleotide derivatives of G to A nucleotides, and in maintaining the intracellular balance of A and G nucleotides By similarity.
Catalytic activity	Inosine 5'-phosphate + NH3 + NADP+ = guanosine 5'-phosphate + NADPH.
Sequence similarities	Belongs to the IMPDH/GMPR family.

Ontologies

Keywords
Ligand	Metal-binding NADP Potassium
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	nucleotide metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	GMP reductase activity Inferred from electronic annotation. Source: EC potassium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 364	364	GMP reductase		PRO_0000093730
Regions
	Nucleotide binding	111 – 134	24	NADP By similarity
Sites
	Active site	189	1	Thioimidate intermediate By similarity
	Metal binding	184	1	Potassium; via carbonyl oxygen By similarity
	Metal binding	186	1	Potassium; via carbonyl oxygen By similarity
	Binding site	222	1	NADP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9NJD8-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 64224E6F9B14847A	FASTA	364	39,874
	10 20 30 40 50 60 MPTIENEPKL DFKDVLLRPK RSTLKSRADV DLVREFVFRN SKKKYVGIPI VASNMDTVGT 70 80 90 100 110 120 FEVAESLSKK RLFTTIHKHY SVDQWMEFVN RISSNQDILS QIGISSGISD YDFTKLKKIC 130 140 150 160 170 180 GLIPELQYIC LDVANGYSEV FVDFIRRVRE EFPRHTIFAG NVVTGEMTEE LILSGADVVK 190 200 210 220 230 240 VGIGSGSVCT TRKKAGVGYP QLSAVLECAD ASHGLNGHVM SDGGCTNPGD VAKALGAGAD 250 260 270 280 290 300 FVMIGGLFAG HDQCGGDTVE KDGQKYKLFY GMSSDTAMEK HEGSVAEYRA SEGKTITVPY 310 320 330 340 350 360 RGDISKTVQD LLGGLRSACT YTGAKKLKEL SKRATFVRVT QQTNEQYATF EISPSELQNL NIAV

References

[1]

"Identification of potential vaccine and drug target candidates by expressed sequence tag analysis and immunoscreening of Onchocerca volvulus larval cDNA libraries." Lizotte-Waniewski M., Tawe W., Guiliano D.B., Lu W., Liu J., Williams S.A., Lustigman S. Infect. Immun. 68:3491-3501(2000) [PubMed: 10816503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF153721 mRNA. Translation: AAF64252.1.
3D structure databases
SMR	Q9NJD8. Positions 1-341.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.7.1.7. 3103.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR005993. GMP_reduct1. IPR015875. IMP_DH/GMP_Rdtase_CS. IPR001093. IMP_DH_GMPRt. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF00478. IMPDH. 1 hit. [Graphical view]
PIRSF	PIRSF000235. GMP_reductase. 1 hit.
TIGRFAMs	TIGR01305. GMP_reduct_1. 1 hit.
PROSITE	PS00487. IMP_DH_GMP_RED. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GMPR_ONCVO

Accession

Primary (citable) accession number: Q9NJD8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	October 1, 2000
Last modified:	February 9, 2010
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)