Reviewed, UniProtKB/Swiss-Prot Q9NJB5 (ONEC_DROME)

Names and origin

Protein names

Recommended name:
    Homeobox protein onecut

Gene names

Name:	onecut
ORF Names:	CG1922

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	1081 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Transcriptional regulator. Binds and recognize ATTG sites.
Subcellular location	Nucleus.
Tissue specificity	Neural-specific.
Sequence similarities	Belongs to the CUT homeobox family. Contains 1 CUT DNA-binding domain. Contains 1 homeobox DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Homeobox
Ligand	DNA-binding
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1081	1081	Homeobox protein onecut		PRO_0000202407
Regions
	DNA binding	751 – 837	87	CUT
	DNA binding	918 – 977	60	Homeobox
Experimental info
	Sequence conflict	104	1	R → T in AAF25796. Ref.1
	Sequence conflict	132	1	D → H in AAF25796. Ref.1
	Sequence conflict	305	1	R → A in AAF25796. Ref.1
	Sequence conflict	497	1	R → K in AAF25796. Ref.1
	Sequence conflict	847	1	S → R in AAF25796. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9NJB5-1 [UniParc]. Last modified April 27, 2001. Version 2. Checksum: 2913672792F194DA	FASTA	1,081	117,698
	10 20 30 40 50 60 MDSLNDIIDH QTFSQELVED ASEFITVGHH SERPSQSSQQ PNSGQDLTMS MQDIISCPVK 70 80 90 100 110 120 HRTCSASGSG SASGSDSVVM VIDALGQGNR QSAYQIVPQQ LQQRNMPLPF GLLERDRQHM 130 140 150 160 170 180 QHGREVNTSP VDFVSSDINL DGLTVDADVS QTDHSQETAV KQEQKLLIVQ SKSQDQSHRR 190 200 210 220 230 240 IRMLVDVSSV NSGLGVHVDD MDEISSDGVG CDDEGVTLSH QHLLEQEEQF GLTSHHPHLQ 250 260 270 280 290 300 PHTQIIHGLH QRSTHSEMGL DNGHGEVLSV IVHSQDSDKE DCEENDDGDA EGDLENEDDD 310 320 330 340 350 360 ERDSRSREQL LSHSSYQTLT SVNDRLSSPG FSQTSYATLT PIQPLPPIST MSEKFAYSGH 370 380 390 400 410 420 ISGGDSGDTD VNGDGAGGGV VEVGEVTNQS SEATGTVSIS SGNATSSVCS NNDCSSFSAL 430 440 450 460 470 480 SMPIGSGHLG LGVLSGVQSP FSSYEKLSSM ISPPPNNYLV SCDLHSSVSG RVINSSHLQL 490 500 510 520 530 540 SHNGNKKESG THEHTHRPAD VNGGKFSYTG HISRGDSVDN DVNGEKFSFS DHISGGDSGD 550 560 570 580 590 600 EDANREKFIY SDHISEGENG PDVNSGTNWL QMHSEREVRL HMPVPAELEA RFHISSERRT 610 620 630 640 650 660 RLNVPPARGS LSRHLAPNAP ICPADWKADD WKHSNAGVVS LTADMPVVVS LTPTPPPLRD 670 680 690 700 710 720 DSVSGIKQNE RSSPGHREQY FLDKSQEPSS VVADQCSPGI NGTPQSVCVI HQQSPSALGN 730 740 750 760 770 780 GFQSSSQQAK VSSCASPKGT VSSGGAVSNR IANSSDMEEI NTKDLAQRIS AELKRYSIPQ 790 800 810 820 830 840 AIFAQRVLCR SQGTLSDLLR NPKPWSKLKS GRETFRRMYK WLQEPEFQRM SALRMAAAQI 850 860 870 880 890 900 PQRAPLSSGM SLGSATGPSG STGTIPTDLD PHGGPTMIQN PLTNESDSSP ASTPVTSVLV 910 920 930 940 950 960 GSVVSCRRKE EPQIEQMPQP KKPRLVFTDL QRRTLQAIFK ETKRPSKEMQ VTIARQLGLE 970 980 990 1000 1010 1020 PTTVGNFFMN ARRRSMDKWR DDDSKSTMHV AHSRQQQDEQ QKDNAHTHSQ SQIQDQNHSQ 1030 1040 1050 1060 1070 1080 NQAIHNSMSQ DPYSNLHTTA MSPLGAFDED ADMDLELESH DFDLVDPDEH GDTNDPNGDM L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Drosophila onecut is a neural-specific transcriptional regulator." Nguyen D.N.T., Lai Z.C. Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF169227 mRNA. Translation: AAF25796.1. AE014135 Genomic DNA. Translation: AAF59330.2.
RefSeq	NP_524842.2.
UniGene	Dm.1788
3D structure databases
SMR	Q9NJB5. Positions 757-835.
ModBase	Search...
Genome annotation databases
Ensembl	FBgn0028996. Drosophila melanogaster. [Contig view]
GeneID	45816.
KEGG	dme:Dmel_CG1922.
NMPDR	fig|7227.3.peg.8420.
Organism-specific databases
FlyBase	FBgn0028996. onecut.
Phylogenomic databases
HOGENOM	Q9NJB5.
OMA	Q9NJB5. SMISPPP.
Enzyme and pathway databases
BioCyc	DMEL-XXX-02:DMEL-XXX-02-018012-MON.
Gene expression databases
ArrayExpress	Q9NJB5.
GermOnline	CG1922. Drosophila melanogaster.
Family and domain databases
InterPro	IPR003350. Hmoeo_CUT. IPR001356. Homeobox. IPR017970. Homeobox_CS. IPR012287. Homeodomain-rel. [Graphical view]
Gene3D	G3DSA:1.10.10.60. Homeodomain-rel. 1 hit.
Pfam	PF02376. CUT. 1 hit. PF00046. Homeobox. 1 hit. [Graphical view]
ProDom	PD000010. Homeobox. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00389. HOX. 1 hit. [Graphical view]
PROSITE	PS51042. CUT. 1 hit. PS00027. HOMEOBOX_1. False negative. PS50071. HOMEOBOX_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	838367.

Entry information

Entry name

ONEC_DROME

Accession

Primary (citable) accession number: Q9NJB5
Secondary accession number(s): Q9V4E4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	April 27, 2001
Last modified:	June 16, 2009
This is version 61 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project