Q9NJ98 (BGBP1_MANSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Beta-1,3-glucan-binding protein 1 Short name=BGBP-1 Alternative name(s): Beta-1,3-glucan recognition protein 1 Short name=BetaGRP-1
Organism	Manduca sexta (Tobacco hawkmoth) (Tobacco hornworm)
Taxonomic identifier	7130 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Lepidoptera › Glossata › Ditrysia › Bombycoidea › Sphingidae › Sphinginae › Sphingini › Manduca

Protein attributes

Sequence length	487 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the recognition of invading micro-organisms. Binds specifically to beta-1,3-glucan and activates the phenoloxidase cascade. Causes aggregation of invading micro-organisms. Ref.1
Subunit structure	Monomer. Ref.1
Subcellular location	Secreted Ref.1.
Tissue specificity	Fat body and hemolymph. Ref.1
Developmental stage	Expression is maintained at a moderate level during larval feeding and wandering stages. Ref.2
Post-translational modification	N-glycosylated. Ref.1
Sequence similarities	Belongs to the insect beta-1,3-glucan binding protein family.
Mass spectrometry	Molecular mass is 53583 Da from positions 20 - 487. Determined by MALDI. Ref.1

Ontologies

Keywords
Biological process	Immunity Innate immunity
Cellular component	Secreted
Domain	Signal
PTM	Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro innate immune response Inferred from electronic annotation. Source: UniProtKB-KW regulation of innate immune response Inferred from direct assay Ref.1. Source: UniProtKB
Cellular component	extracellular region Inferred from direct assay Ref.1. Source: UniProtKB
Molecular function	bacterial cell surface binding Inferred from direct assay Ref.1. Source: UniProtKB hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Ref.1
	Chain	20 – 487	468	Beta-1,3-glucan-binding protein 1 Ref.1		PRO_0000002821
Amino acid modifications
	Glycosylation	368	1	N-linked (GlcNAc...) Probable

Sequences

	Sequence		Length	Mass (Da)
	Q9NJ98 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 3BA4869C5ADE934A	FASTA	487	54,567
	10 20 30 40 50 60 MLKSVFVLFL VNYLNSVRCL EVPDAKLEAI YPKGLRVSIP DDGYTLFAFH GKLNEEMEGL 70 80 90 100 110 120 EAGHWSRDIT KAKNGRWIFR DRNAKLKIGD KIYFWTYILK DGLGYRQDNG EWTVTGYVNE 130 140 150 160 170 180 DGEPLDANFE PRSTASTAAP PQAGAGQAPG PSYPCELSVS EVSVPGFVCK GQMLFEDNFN 190 200 210 220 230 240 KPLADGRIWT PEIMFPGEPD YPFNVYMKET DNLHVGNGNL VIKPMPLVTA FGEDAIWKTL 250 260 270 280 290 300 DLSDRCTGLL GTAQCKRDPS DAIIVPPIVT AKINTKKTFA FKYGRVEISA KMPRGDWLVP 310 320 330 340 350 360 LIQLEPVNKN YGIRNYVSGL LRVACVKGNT EYIKTLVGGP IMSEAEPYRT ANLKEFISNE 370 380 390 400 410 420 PWTNEFHNYT LEWSPDAITM AVDGIVYGRV TAPAGGFYKE ANEQNVEAAA RWIQGSNIAP 430 440 450 460 470 480 FDDMFYISLG MDVGGVHEFP DEAINKPWKN TATKAMVNFW NARSQWNPTW LESEKALLVD YVRVYAL

References

[1]

"A beta1,3-glucan recognition protein from an insect, Manduca sexta, agglutinates microorganisms and activates the phenoloxidase cascade." Ma C., Kanost M.R. J. Biol. Chem. 275:7505-7514(2000) [PubMed: 10713054] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 20-42, FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MASS SPECTROMETRY, GLYCOSYLATION. Tissue: Fat body and Larval hemolymph.

[2]

"Beta-1,3-glucan recognition protein-2 (betaGRP-2) from Manduca sexta: an acute-phase protein that binds beta-1,3-glucan and lipoteichoic acid to aggregate fungi and bacteria and stimulate prophenoloxidase activation." Jiang H., Ma C., Lu Z.-Q., Kanost M.R. Insect Biochem. Mol. Biol. 34:89-100(2004) [PubMed: 14976985] [Abstract] Cited for: DEVELOPMENTAL STAGE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF177982 mRNA. Translation: AAF44011.1.
3D structure databases
ProteinModelPortal	Q9NJ98.
ModBase	Search...
Protein family/group databases
CAZy	CBM39. Carbohydrate-Binding Module Family 39. GH16. Glycoside Hydrolase Family 16.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR008985. ConA-like_lec_gl. IPR013320. ConA-like_subgrp. IPR000757. Glyco_hydro_16. [Graphical view]
Gene3D	G3DSA:2.60.120.200. ConA_like_subgrp. 1 hit.
Pfam	PF00722. Glyco_hydro_16. 1 hit. [Graphical view]
SUPFAM	SSF49899. ConA_like_lec_gl. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BGBP1_MANSE

Accession

Primary (citable) accession number: Q9NJ98

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2004
Last sequence update:	October 1, 2000
Last modified:	January 11, 2011
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)