Q9NIP6 (CP2B_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cardio acceleratory peptide 2b

Alternative name(s):
Capability protein
Myotropin-CAP2b-like protein

Cleaved into the following 3 chains:

1. CAP-1
   Alternative name(s):
   CAP2b-1
   Capa-1
2. CAP-2
   Alternative name(s):
   CAP2b-2
   Capa-2
3. CAP-3
   Alternative name(s):
   CAP2b-3
   Capa-3
   Myotropin
   Pyrokinin-1

Gene names

Name:	capa
Synonyms:	MT-CAP2b
ORF Names:	CG15520

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	151 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	CAP-1 and CAP-2, but not CAP-3 are ligands for the Capa receptor. CAP-1 and CAP-2 are probably components of the signal transduction pathway that leads to Malpighian tubule fluid secretion via the second messenger nitric oxide. CAP-3 may be a ligand for the CG8795 G protein coupled receptors. Ref.1 Ref.7 Ref.8
Subcellular location	Secreted. Note: Released from the neuroendocrine cells into the hemolymph. Ref.1
Tissue specificity	In larvae, the precursor peptide is exclusively present in a single pair of neuroendocrine cells in the labial neuromere (subesophageal ganglion) and three pairs of cells in the ventral ganglion abdominal neuromeres. Ref.1
Sequence similarities	Belongs to the pyrokinin family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Neuropeptide
PTM	Amidation Cleavage on pair of basic residues
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	cation transport Traceable author statement. Source: FlyBase epithelial fluid transport Inferred from direct assay Ref.1. Source: FlyBase neuropeptide signaling pathway Inferred from mutant phenotype Ref.1. Source: UniProtKB
Cellular component	extracellular region Inferred from direct assay Ref.1. Source: UniProtKB
Molecular function	myostimulatory hormone activity Inferred from mutant phenotype Ref.1. Source: UniProtKB neuropeptide hormone activity Inferred from mutant phenotype Ref.1. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CG7191	Q9VLX6	1	EBI-155503,EBI-121750

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Propeptide	22 – 28	7			PRO_0000029901
	Peptide	31 – 42	12	CAP-1 Ref.6		PRO_0000029902
	Propeptide	45 – 80	36			PRO_0000029903
	Peptide	83 – 92	10	CAP-2		PRO_0000029904
	Propeptide	95 – 113	19			PRO_0000029905
	Peptide	116 – 130	15	CAP-3		PRO_0000029906
	Propeptide	134 – 151	18			PRO_0000029907
Amino acid modifications
	Modified residue	42	1	Valine amide
	Modified residue	92	1	Valine amide
	Modified residue	130	1	Leucine amide

Sequences

	Sequence		Length	Mass (Da)
	Q9NIP6 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: F9E97535665B37E5	FASTA	151	16,507
	10 20 30 40 50 60 MKSMLVHIVL VIFIIAEFST AETDHDKNRR GANMGLYAFP RVGRSDPSLA NSLRDGLEAG 70 80 90 100 110 120 VLDGIYGDAS QEDYNEADFQ KKASGLVAFP RVGRGDAELR KWAHLLALQQ VLDKRTGPSA 130 140 150 SSGLWFGPRL GKRSVDAKSF ADISKGQKEL N

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Two nitridergic peptides are encoded by the gene capability in Drosophila melanogaster." Kean L., Cazenave W., Costes L., Broderick K.E., Graham S., Pollock V.P., Davies S.A., Veenstra J.A., Dow J.A. Am. J. Physiol. 282:R1297-R1307(2002) [PubMed: 11959669] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[2]	Vanden Broeck J.J.M. Submitted (NOV-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[5]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Head.
[6]	"Peptidomics of the larval Drosophila melanogaster central nervous system." Baggerman G., Cerstiaens A., De Loof A., Schoofs L. J. Biol. Chem. 277:40368-40374(2002) [PubMed: 12171930] [Abstract] Cited for: PROTEIN SEQUENCE OF 31-42 AND 83-92, AMIDATION. Tissue: Larva.
[7]	"Identification of G protein-coupled receptors for Drosophila PRXamide peptides, CCAP, corazonin, and AKH supports a theory of ligand-receptor coevolution." Park Y., Kim Y.-J., Adams M.E. Proc. Natl. Acad. Sci. U.S.A. 99:11423-11428(2002) [PubMed: 12177421] [Abstract] Cited for: FUNCTION. Strain: Canton-S.
[8]	"Molecular cloning and functional expression of a Drosophila receptor for the neuropeptides capa-1 and -2." Iversen A., Cazzamali G., Williamson M., Hauser F., Grimmelikhuijzen C.J.P. Biochem. Biophys. Res. Commun. 299:628-633(2002) [PubMed: 12459185] [Abstract] Cited for: FUNCTION.
[9]	"Neuropeptides and their precursors in the fruitfly, Drosophila melanogaster." Vanden Broeck J.J.M. Peptides 22:241-254(2001) [PubMed: 11179818] [Abstract] Cited for: REVIEW.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF203878 mRNA. Translation: AAF62876.1. AJ291724 mRNA. Translation: CAC17603.1. AE014297 Genomic DNA. Translation: AAF56969.2. AY069234 mRNA. Translation: AAL39379.1.
RefSeq	NP_524552.1. NM_079828.2.
UniGene	Dm.2221.
3D structure databases
ProteinModelPortal	Q9NIP6.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-22273N.
IntAct	Q9NIP6. 1 interaction.
MINT	MINT-783841.
STRING	Q9NIP6.
Proteomic databases
PRIDE	Q9NIP6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0085514; FBpp0084880; FBgn0039722.
GeneID	43541.
KEGG	dme:Dmel_CG15520.
NMPDR	fig|7227.3.peg.15331.
Organism-specific databases
CTD	43541.
FlyBase	FBgn0039722. capa.
Phylogenomic databases
eggNOG	inNOG11760.
GeneTree	EMGT00050000017710.
InParanoid	Q9NIP6.
OMA	KNRRGAN.
OrthoDB	EOG48SF9H.
PhylomeDB	Q9NIP6.
Gene expression databases
Bgee	Q9NIP6.
GermOnline	CG15520. Drosophila melanogaster.
Family and domain databases
PROSITE	PS00539. PYROKININ. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	834452.

Entry information

Entry name

CP2B_DROME

Accession

Primary (citable) accession number: Q9NIP6
Secondary accession number(s): Q9VAE2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 17, 2003
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project