Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Potassium channel toxin alpha-KTx 1.6

Gene
N/A
Organism
Mesobuthus martensii (Manchurian scorpion) (Buthus martensii)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Potent blocker of both large-conductance calcium-activated potassium channels (BKCa channels) and voltage-gated potassium channels (Kv1.3/KCNA3 (PubMed:9354615) and ERG1/Kv11.1/KCNH2 (PubMed:18687312)).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei48 – 481Basic residue of the functional dyadBy similarity
Sitei57 – 571Aromatic residue of the functional dyadBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Calcium-activated potassium channel impairing toxin, Ion channel impairing toxin, Neurotoxin, Potassium channel impairing toxin, Toxin, Voltage-gated potassium channel impairing toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium channel toxin alpha-KTx 1.6
Alternative name(s):
BmTX21 Publication
Neurotoxin TX2
OrganismiMesobuthus martensii (Manchurian scorpion) (Buthus martensii)
Taxonomic identifieri34649 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaScorpionesButhidaButhoideaButhidaeMesobuthus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 21211 PublicationAdd
BLAST
Chaini22 – 5837Potassium channel toxin alpha-KTx 1.61 PublicationPRO_0000035318Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei22 – 221Pyrrolidone carboxylic acid
Disulfide bondi28 ↔ 491 Publication
Disulfide bondi34 ↔ 541 Publication
Disulfide bondi38 ↔ 561 Publication

Keywords - PTMi

Disulfide bond, Pyrrolidone carboxylic acid

Expressioni

Tissue specificityi

Expressed by the venom gland.Curated

Structurei

Secondary structure

1
58
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi23 – 275Combined sources
Helixi32 – 343Combined sources
Helixi35 – 417Combined sources
Beta strandi47 – 504Combined sources
Beta strandi53 – 564Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2BMTNMR-A23-58[»]
ProteinModelPortaliQ9NII5.
SMRiQ9NII5. Positions 22-58.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9NII5.

Family & Domainsi

Domaini

Has the structural arrangement of an alpha-helix connected to a beta-sheet by disulfide bonds (CSalpha/beta).

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.30.30.10. 1 hit.
InterProiIPR003614. Scorpion_toxin-like.
IPR001947. Scorpion_toxinS_K_inh.
[Graphical view]
PfamiPF00451. Toxin_2. 1 hit.
[Graphical view]
PRINTSiPR00286. CHARYBDTOXIN.
ProDomiPD003586. Scorpion_toxinS. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF57095. SSF57095. 1 hit.
PROSITEiPS01138. SCORP_SHORT_TOXIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9NII5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKISFLLLLA IVICSIGWTE AQFTNVSCSA SSQCWPVCKK LFGTYRGKCM

NSKCRCYS
Length:58
Mass (Da):6,507
Last modified:October 1, 2000 - v1
Checksum:iF19AA7351B9708B8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF208300 Genomic DNA. Translation: AAF63972.1.
AF151537 mRNA. Translation: AAQ13576.1.
AF247058 Genomic DNA. Translation: AAK73518.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF208300 Genomic DNA. Translation: AAF63972.1.
AF151537 mRNA. Translation: AAQ13576.1.
AF247058 Genomic DNA. Translation: AAK73518.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2BMTNMR-A23-58[»]
ProteinModelPortaliQ9NII5.
SMRiQ9NII5. Positions 22-58.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiQ9NII5.

Family and domain databases

Gene3Di3.30.30.10. 1 hit.
InterProiIPR003614. Scorpion_toxin-like.
IPR001947. Scorpion_toxinS_K_inh.
[Graphical view]
PfamiPF00451. Toxin_2. 1 hit.
[Graphical view]
PRINTSiPR00286. CHARYBDTOXIN.
ProDomiPD003586. Scorpion_toxinS. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF57095. SSF57095. 1 hit.
PROSITEiPS01138. SCORP_SHORT_TOXIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genomic organization of three novel toxins from the scorpion Buthus martensi Karsch that are active on potassium channels."
    Dai L., Wu J.-J., Gu Y.-H., Lan Z.-D., Ling M.-H., Chi C.-W.
    Biochem. J. 346:805-809(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Molecular cloning and genomic organization of a K(+) channel toxin from the Chinese scorpion Buthus martensii Karsch."
    Zeng X.-C., Zhu Z.-H., Li W.-X., Zhu S.-Y., Peng F., Mao X., Liu H.
    Toxicon 39:407-410(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
  3. "Purification, characterization, and synthesis of three novel toxins from the Chinese scorpion Buthus martensi, which act on K+ channels."
    Romi-Lebrun R., Lebrun B., Martin-Eauclaire M.-F., Ishiguro M., Escoubas P., Wu F.Q., Hisada M., Pongs O., Nakajima T.
    Biochemistry 36:13473-13482(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 22-58, SYNTHESIS OF 22-58, FUNCTION, SUBCELLULAR LOCATION, PYROGLUTAMATE FORMATION AT GLN-22.
    Tissue: Venom.
  4. "A common 'hot spot' confers hERG blockade activity to alpha-scorpion toxins affecting K+ channels."
    Abdel-Mottaleb Y., Corzo G., Martin-Eauclaire M.F., Satake H., Ceard B., Peigneur S., Nambaru P., Bougis P.E., Possani L.D., Tytgat J.
    Biochem. Pharmacol. 76:805-815(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Solution structure of two new toxins from the venom of the Chinese scorpion Buthus martensi Karsch blockers of potassium channels."
    Blanc E., Romi-Lebrun R., Bornet O., Nakajima T., Darbon H.
    Biochemistry 37:12412-12418(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 23-58, DISULFIDE BONDS, SYNTHESIS OF 23-58.

Entry informationi

Entry nameiKAX16_MESMA
AccessioniPrimary (citable) accession number: Q9NII5
Secondary accession number(s): P58489
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: October 1, 2000
Last modified: June 24, 2015
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Scorpion potassium channel toxins
    Nomenclature of scorpion potassium channel toxins and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.