Q9NHV9 (VAV_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein vav
Short name=DroVav
Short name=dVAV

Gene names

Name:	vav
ORF Names:	CG7893

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	793 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Couples tyrosine kinase signals with the activation of the Rho/Rac GTPases. Probably plays a pivotal role as a signal transducer protein during fruit fly development.
Tissue specificity	Ubiquitous.
Developmental stage	Expressed both maternally and zygotically in all stages of development.
Post-translational modification	Phosphorylated on tyrosine residues.
Sequence similarities	Contains 1 CH (calponin-homology) domain. Contains 1 DH (DBL-homology) domain. Contains 1 PH domain. Contains 1 phorbol-ester/DAG-type zinc finger. Contains 1 SH2 domain. Contains 1 SH3 domain.
Sequence caution	The sequence AAM12266.1 differs from that shown. Reason:

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Domain	Repeat SH2 domain SH3 domain Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Guanine-nucleotide releasing factor
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	actin cytoskeleton reorganization Inferred from direct assay PubMed 15950967. Source: FlyBase actin filament organization Inferred from mutant phenotype PubMed 14527345. Source: FlyBase cell adhesion Inferred from mutant phenotype PubMed 14527345. Source: FlyBase cell migration Inferred from mutant phenotype PubMed 15950967. Source: FlyBase central nervous system development Inferred from genetic interaction PubMed 20147552. Source: FlyBase dorsal closure Inferred from mutant phenotype PubMed 15950967. Source: FlyBase intracellular signal transduction Inferred from electronic annotation. Source: InterPro melanotic encapsulation of foreign target Inferred from mutant phenotype PubMed 21947570. Source: FlyBase myoblast fusion Inferred from mutant phenotype PubMed 15950967. Source: FlyBase open tracheal system development Inferred from mutant phenotype PubMed 15950967. Source: FlyBase photoreceptor cell axon guidance Inferred from mutant phenotype PubMed 20147552. Source: FlyBase regulation of Rho protein signal transduction Inferred from electronic annotation. Source: InterPro regulation of cell shape Inferred from mutant phenotype PubMed 14527345. Source: FlyBase
Cellular_component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular_function	Rho guanyl-nucleotide exchange factor activity Inferred from electronic annotation. Source: InterPro guanyl-nucleotide exchange factor activity Inferred from direct assay PubMed 15950967. Source: FlyBase metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phospholipid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform A (identifier: Q9NHV9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform B (identifier: Q9NHV9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-171: MASSSSSNSF...EAIYKDLHST → MRRMRASRRP...LPSRRRPNPS

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 793	793	Protein vav		PRO_0000080983
Regions
	Domain	18 – 136	119	CH
	Domain	216 – 396	181	DH
	Domain	432 – 541	110	PH
	Domain	622 – 726	105	SH2
	Domain	726 – 788	63	SH3
	Zinc finger	552 – 601	50	Phorbol-ester/DAG-type
Natural variations
	Alternative sequence	1 – 171	171	MASSS…DLHST → MRRMRASRRPCFPTARAAMC ASYPSISPWMWLAPRRMPHP MRSHPRRNRMPANRNHRWPP QRQAFLCPLHRGHLWAVWCP PPPHRRRFWSARASGCSERP PPSTTTAPRWRPPSTSMPTS TARTTRRSTRICATLPSRRR PNPS in isoform B.		VSP_027801
Experimental info
	Sequence conflict	53	1	T → M in AAF28765. Ref.1
	Sequence conflict	148	1	F → L in AAF28765. Ref.1
	Sequence conflict	372	1	L → M in AAF28765. Ref.1
	Sequence conflict	503	1	R → E in AAF28765. Ref.1
	Sequence conflict	645 – 664	20	LLRVR…MYALS → PVASSSAGPIHCPRDDVCAY in AAF28765. Ref.1
	Sequence conflict	739	1	P → Q in AAF28765. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform A [UniParc]. Last modified July 11, 2002. Version 2. Checksum: 1031929FE4FB8BF3	FASTA	793	91,355
	10 20 30 40 50 60 MASSSSSNSF GGVAGVNGDL WRECVAWLTR CKVIPPDHKA AQPDAEIRIL AMTLRDGVLL 70 80 90 100 110 120 CNLVIHLDPS SLDPREFNRK PQMAQFLCSK NIKLFLDVCH NNFGIRDADL FEPTMLYDLT 130 140 150 160 170 180 NFHRVLITLS KLSQCRKVQQ LHPDLIGFNL QLSPTERSHS DEAIYKDLHS TTTDNIACNG 190 200 210 220 230 240 TGYDHTNTKE EEVYQDLCAL HRTSRSQTAS STSFEQRDYV IRELIDTESN YLDVLTALKT 250 260 270 280 290 300 KFMGPLERHL NQDELRLIFP RIRELVDIHT KFLDKLRESL TPNAKVKMAQ VFLDFREPFL 310 320 330 340 350 360 IYGEFCSLLL GAIDYLADVC KKNQIIDQLV QKCERDYNVG KLQLRDILSV PMQRILKYHL 370 380 390 400 410 420 LLDKLVKETS PLHDDYRSLE RAKEAMIDVS QYINEVKRDS DHLVIIQKVK DSICDIHLLQ 430 440 450 460 470 480 NGNGSDLLQY GRLLLDGELH IKAHEDQKTK LRYAFVFDKI LIMVKALHIK TGDMQYTYRD 490 500 510 520 530 540 SHNLADYRVE QSHSRRTIGR DTRFKYQLLL ARKSGKTAFT LYLKSEHERD KWRKALTEAM 550 560 570 580 590 600 ESLEPPGCQS TDHKMEIYTF DAPTTCRHCS KFLKGRIHQG YRCKVCQISV HKGCISSTGR 610 620 630 640 650 660 CKQNPVSVPP PVCDRQLSEF NWFAGNMDRE TAAHRLENRR IGTYLLRVRP QGPSTAHETM 670 680 690 700 710 720 YALSLKTDDN VIKHMKINQE NSGDSMLYCL SSRRHFKTIV ELVSYYERND LGENFAGLNQ 730 740 750 760 770 780 SLQWPYKEVI ATALYDYEPK AGSNQLQLRT DCQVLVIGKD GDSKGWWRGK IGDTVGYFPK 790 EYVQEQKLAS EEL
	Isoform B [UniParc]. Checksum: A53E704D4DEEF085	FASTA	766	88,953
	10 20 30 40 50 60 MRRMRASRRP CFPTARAAMC ASYPSISPWM WLAPRRMPHP MRSHPRRNRM PANRNHRWPP 70 80 90 100 110 120 QRQAFLCPLH RGHLWAVWCP PPPHRRRFWS ARASGCSERP PPSTTTAPRW RPPSTSMPTS 130 140 150 160 170 180 TARTTRRSTR ICATLPSRRR PNPSTTDNIA CNGTGYDHTN TKEEEVYQDL CALHRTSRSQ 190 200 210 220 230 240 TASSTSFEQR DYVIRELIDT ESNYLDVLTA LKTKFMGPLE RHLNQDELRL IFPRIRELVD 250 260 270 280 290 300 IHTKFLDKLR ESLTPNAKVK MAQVFLDFRE PFLIYGEFCS LLLGAIDYLA DVCKKNQIID 310 320 330 340 350 360 QLVQKCERDY NVGKLQLRDI LSVPMQRILK YHLLLDKLVK ETSPLHDDYR SLERAKEAMI 370 380 390 400 410 420 DVSQYINEVK RDSDHLVIIQ KVKDSICDIH LLQNGNGSDL LQYGRLLLDG ELHIKAHEDQ 430 440 450 460 470 480 KTKLRYAFVF DKILIMVKAL HIKTGDMQYT YRDSHNLADY RVEQSHSRRT IGRDTRFKYQ 490 500 510 520 530 540 LLLARKSGKT AFTLYLKSEH ERDKWRKALT EAMESLEPPG CQSTDHKMEI YTFDAPTTCR 550 560 570 580 590 600 HCSKFLKGRI HQGYRCKVCQ ISVHKGCISS TGRCKQNPVS VPPPVCDRQL SEFNWFAGNM 610 620 630 640 650 660 DRETAAHRLE NRRIGTYLLR VRPQGPSTAH ETMYALSLKT DDNVIKHMKI NQENSGDSML 670 680 690 700 710 720 YCLSSRRHFK TIVELVSYYE RNDLGENFAG LNQSLQWPYK EVIATALYDY EPKAGSNQLQ 730 740 750 760 LRTDCQVLVI GKDGDSKGWW RGKIGDTVGY FPKEYVQEQK LASEEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of the Drosophila melanogaster homologue of the mammalian signal transducer protein, Vav." Dekel I., Russek N., Jones T., Mortin M.A., Katzav S. FEBS Lett. 472:99-104(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A). Tissue: Embryo.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING. Strain: Berkeley.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A). Strain: Berkeley. Tissue: Embryo.
[5]	Stapleton M., Carlson J.W., Frise E., Kapadia B., Park S., Wan K.H., Yu C., Celniker S.E. Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B). Strain: Berkeley. Tissue: Head, Larva and Pupae.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF218780 mRNA. Translation: AAF28765.1. AE014298 Genomic DNA. Translation: AAF48943.3. AE014298 Genomic DNA. Translation: AAN09485.1. AY069536 mRNA. Translation: AAL39681.1. AY095173 mRNA. Translation: AAM12266.1. Sequence problems. BT029984 mRNA. Translation: ABM92858.1.
RefSeq	NP_573372.1. NM_133144.4. NP_728235.1. NM_167642.3.
UniGene	Dm.218.
3D structure databases
ProteinModelPortal	Q9NHV9.
SMR	Q9NHV9. Positions 12-601, 618-787.
ModBase	Search...
Proteomic databases
PaxDb	Q9NHV9.
PRIDE	Q9NHV9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0074677; FBpp0074448; FBgn0040068. FBtr0074678; FBpp0074449; FBgn0040068.
GeneID	32920.
KEGG	dme:Dmel_CG7893.
UCSC	CG7893-RA. d. melanogaster.
Organism-specific databases
CTD	32920.
FlyBase	FBgn0040068. vav.
Phylogenomic databases
eggNOG	NOG326494.
GeneTree	ENSGT00700000104383.
InParanoid	Q9NHV9.
KO	K05730.
OrthoDB	EOG4W3R30.
Gene expression databases
Bgee	Q9NHV9.
GermOnline	CG7893. Drosophila melanogaster.
Family and domain databases
Gene3D	1.10.418.10. 1 hit. 1.20.900.10. 1 hit. 2.30.29.30. 1 hit. 3.30.505.10. 1 hit.
InterPro	IPR001715. CH-domain. IPR000219. DH-domain. IPR011993. PH_like_dom. IPR001849. Pleckstrin_homology. IPR002219. Prot_Kinase_C-like_PE/DAG-bd. IPR000980. SH2. IPR001452. SH3_domain. [Graphical view]
Pfam	PF00130. C1_1. 1 hit. PF00307. CH. 1 hit. PF00621. RhoGEF. 1 hit. PF00017. SH2. 1 hit. PF00018. SH3_1. 1 hit. [Graphical view]
SMART	SM00109. C1. 1 hit. SM00033. CH. 1 hit. SM00233. PH. 1 hit. SM00325. RhoGEF. 1 hit. SM00252. SH2. 1 hit. SM00326. SH3. 1 hit. [Graphical view]
SUPFAM	SSF47576. Calponin-homology. 1 hit. SSF48065. DH-domain. 1 hit.
PROSITE	PS50021. CH. 1 hit. PS00741. DH_1. False negative. PS50010. DH_2. 1 hit. PS50003. PH_DOMAIN. 1 hit. PS50001. SH2. 1 hit. PS50002. SH3. 1 hit. PS00479. ZF_DAG_PE_1. 1 hit. PS50081. ZF_DAG_PE_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	32920.
NextBio	781038.

Entry information

Entry name

VAV_DROME

Accession

Primary (citable) accession number: Q9NHV9
Secondary accession number(s): A2RVJ3 , A4V4R6, Q8SWT3, Q8T061, Q9VWJ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2002
Last sequence update:	July 11, 2002
Last modified:	May 1, 2013
This is version 107 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project