Reviewed, UniProtKB/Swiss-Prot Q9NHB0 (BGBP1_DROME)

Names and origin

Protein names

Recommended name:
    Gram-negative bacteria-binding protein 1

Gene names

Name:	GNBP1
ORF Names:	CG6895

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	494 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in the recognition of invading micro-organisms. Binds specifically to beta-1,3-glucan and lipopolysaccharide and induces expression of the antimicrobial peptides drosomycin, cecropin and attacin. Ref.1
Subcellular location	Cell membrane; Lipid-anchor › GPI-anchor. Note: Secreted and attached to the membrane by a GPI-anchor. Ref.1
Developmental stage	Expressed at moderate levels throughout the life cycle. Ref.1
Sequence similarities	Belongs to the insect beta-1,3-glucan binding protein family.
Sequence caution	The sequence AAF33849.1 differs from that shown. Reason: Frameshift at positions 457 and 468.

Ontologies

Keywords
Biological process	Immune response Innate immunity
Cellular component	Cell membrane Membrane
Domain	Signal
PTM	GPI-anchor Glycoprotein Lipoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	defense response to Gram-positive bacterium Inferred from mutant phenotype. Source: FlyBase innate immune response Non-traceable author statement. Source: FlyBase peptidoglycan catabolic process Inferred from direct assay. Source: FlyBase regulation of innate immune response Ref.1 Inferred from direct assay. Source: UniProtKB
Cellular component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-KW extracellular region Ref.1 Inferred from direct assay. Source: UniProtKB membrane fraction Ref.1 Inferred from direct assay. Source: FlyBase plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	bacterial binding Ref.1 Inferred from direct assay. Source: UniProtKB hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro lipopolysaccharide binding Traceable author statement. Source: FlyBase peptidoglycan binding Inferred from direct assay. Source: FlyBase polysaccharide binding Traceable author statement. Source: FlyBase protein binding Inferred from physical interaction. Source: FlyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 494	475	Gram-negative bacteria-binding protein 1		PRO_0000002817
Amino acid modifications
	Glycosylation	56	1	N-linked (GlcNAc...) Potential
	Glycosylation	81	1	N-linked (GlcNAc...) Potential
	Glycosylation	185	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	230	1	P → R in AAF33849. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9NHB0-1 [UniParc]. Last modified September 27, 2004. Version 2. Checksum: 95172030E5E61FAB	FASTA	494	55,531
	10 20 30 40 50 60 MPGLCIGILL LIGFGCTTAY KIPTPTVELL ETGFSVSIPD EEGVKVVAFN VNRNRNFTSF 70 80 90 100 110 120 INEGQYNVRL TEPQNGRWTT NFSSVPLRSQ DVLYLWTSVQ HQKAVYQDLA QPLPVCNLGG 130 140 150 160 170 180 EYRPRGCSPG DDDFTDDNQL STEDSALEPT APSVCEPSES QVSPQIGVSI CKGQLLFEET 190 200 210 220 230 240 FDQLNESLWI HDVRLPLDSK DAEFVLYDGK AKVHDGNLVI EPLLWSSYRP DLSIANSRLD 250 260 270 280 290 300 LSERCTGTHN RIKECILHST GSGPSGIMPP IVTPRISTKE TFAFQYGRIE IRAKLPKGDW 310 320 330 340 350 360 IVPLLLLEPL TEWYGQSGYE SGQLRVALAR GNSVLRMPRG KLVDGRSLYG GPVLSTDAHQ 370 380 390 400 410 420 REDLWLSKRK ISHFGDDFHT YSLDWSSNRL LFSVDGQVYG EMLNGFTELD ENPRWKQGGP 430 440 450 460 470 480 MAPFDKMFYI SLGVSVGGFG DFVDHLRTAT YEKPWANYHP QAKLQFHQAQ DQWLPTWKQP 490 ALKIDYVRVF ATDN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Gram-negative bacteria-binding protein, a pattern recognition receptor for lipopolysaccharide and beta-1,3-glucan that mediates the signaling for the induction of innate immune genes in Drosophila melanogaster cells." Kim Y.-S., Ryu J.-H., Han S.-J., Choi K.-H., Nam K.-B., Jang I.-H., Lemaitre B., Brey P.T., Lee W.-J. J. Biol. Chem. 275:32721-32727(2000) [PubMed: 10827089] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF228472 mRNA. Translation: AAF33849.1. Frameshift. AE014296 Genomic DNA. Translation: AAF49244.1.
RefSeq	NP_524142.2.
UniGene	Dm.2810
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	CBM39. Carbohydrate-Binding Module Family 39. GH16. Glycoside Hydrolase Family 16.
Genome annotation databases
Ensembl	FBgn0040323. Drosophila melanogaster. [Contig view]
GeneID	40034.
KEGG	dme:Dmel_CG6895.
Organism-specific databases
FlyBase	FBgn0040323. GNBP1.
Phylogenomic databases
HOGENOM	Q9NHB0.
Gene expression databases
ArrayExpress	Q9NHB0.
GermOnline	CG6895. Drosophila melanogaster.
Family and domain databases
InterPro	IPR013320. ConA-like_subgrp. IPR000757. Glyco_hydro_16. [Graphical view]
Gene3D	G3DSA:2.60.120.200. ConA_like_subgrp. 1 hit.
Pfam	PF00722. Glyco_hydro_16. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	816659.

Entry information

Entry name

BGBP1_DROME

Accession

Primary (citable) accession number: Q9NHB0
Secondary accession number(s): Q9VVR5

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2004
Last sequence update:	September 27, 2004
Last modified:	June 16, 2009
This is version 49 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project