Q9NHA8 (BGBP3_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gram-negative bacteria-binding protein 3

Gene names

Name:	GNBP3
ORF Names:	CG5008

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	490 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the recognition of invading micro-organisms. Binds specifically to beta-1,3-glucan and activates the phenoloxidase cascade By similarity. UniProtKB Q8MU95
Subcellular location	Secreted By similarity UniProtKB Q8MU95.
Developmental stage	Expressed at very low levels during embryonic development. Expression increases during the larva stages and peaks at a moderate level during the late larval, prepupal and pupal stages, before decreasing in the adult. Ref.1
Sequence similarities	Belongs to the insect beta-1,3-glucan binding protein family.

Ontologies

Keywords
Biological process	Immunity Innate immunity
Cellular component	Secreted
Domain	Signal
PTM	Glycoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro innate immune response Inferred from electronic annotation. Source: UniProtKB-KW regulation of innate immune response Inferred from sequence or structural similarity. Source: UniProtKB response to fungus Non-traceable author statement. Source: FlyBase
Cellular component	extracellular region Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	bacterial cell surface binding Inferred from sequence or structural similarity. Source: UniProtKB hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 25

25

Potential

Chain

26 – 490

465

Gram-negative bacteria-binding protein 3

PRO_0000002819

Amino acid modifications

Glycosylation

362

1

N-linked (GlcNAc...) Potential

Glycosylation

373

1

N-linked (GlcNAc...) Potential

Natural variations

Natural variant

16

1

L → Q in strain: Netherlands line N22.

Natural variant

142

1

P → T in strain: Netherlands line N02 and Netherlands line N30.

Natural variant

213

1

P → L in strain: Netherlands line N02, Netherlands line N07, Netherlands line N17 and Netherlands line N30.

Natural variant

292

1

A → V in strain: Netherlands line N02 and Netherlands line N30.

Natural variant

399

1

K → I in strain: Netherlands line N22.

Natural variant

404

1

E → G in strain: Netherlands line N02 and Netherlands line N30.

Natural variant

408

1

A → G in strain: Netherlands line N22.

Natural variant

479

1

H → N in strain: Netherlands line N22.

Experimental info

Sequence conflict

196

1

R → K in AAF33851. Ref.1

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

490

Helix Strand Turn

Details...

Beta strand

32 – 34

3

Beta strand

42 – 45

4

Beta strand

53 – 57

5

Beta strand

59 – 62

4

Beta strand

74 – 76

3

Beta strand

84 – 87

4

Beta strand

98 – 106

9

Sequences

	Sequence		Length	Mass (Da)
	Q9NHA8 [UniParc]. Last modified July 10, 2007. Version 2. Checksum: B442E16CC027D849	FASTA	490	55,211
	10 20 30 40 50 60 MADALRFVAW SCCLQLLFLL LGVQGYEVPK AKIDVFYPKG FEVSIPDEEG ITLFAFHGKL 70 80 90 100 110 120 NEEMEGLEAG TWARDIVKAK NGRWTFRDRI TALKPGDTLY YWTYVIYNGL GYREDDGSFV 130 140 150 160 170 180 VNGYSGNNAS PHPPVVPVST TPWTPPADPD IDIRLGCTTP KTEVNGAPTR CAGQLVFVDE 190 200 210 220 230 240 FNAAKLDPNK WKAERRFSGQ PDYEFNVYVD DAPETLCLAN GHVVLSTNTM KKQFKKGSGE 250 260 270 280 290 300 SLDLGEKCTG QANTHDCVRN GRTLNDGLPP MVTAQFSSKD FSFKYGRVEV RAKMPRAQWV 310 320 330 340 350 360 TPQIWLQPRR PIYGVDDYRS GQLRIAYTRP NGGNLDLYGA AVLFADEPLR SVKNCLKPGT 370 380 390 400 410 420 GNNSEDWSDS FHNYTLEWTP RELRWLVDGK EWCVQGSAKG SFSETTAAGK SLPQAQKLEE 430 440 450 460 470 480 GTGLAPFDQE FYLTFGLSVG GFNEYQHEIK PWNERAPQAQ KAFWKEVKKI RDHWLDEGHM 490 KIDYVKVYSL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Gram-negative bacteria-binding protein, a pattern recognition receptor for lipopolysaccharide and beta-1,3-glucan that mediates the signaling for the induction of innate immune genes in Drosophila melanogaster cells." Kim Y.-S., Ryu J.-H., Han S.-J., Choi K.-H., Nam K.-B., Jang I.-H., Lemaitre B., Brey P.T., Lee W.-J. J. Biol. Chem. 275:32721-32727(2000) [PubMed: 10827089] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
[2]	"Contrasting evolutionary patterns in Drosophila immune receptors." Jiggins F.M., Kim K.W. J. Mol. Evol. 63:769-780(2006) [PubMed: 17103056] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Netherlands line N01, Netherlands line N02, Netherlands line N03, Netherlands line N06, Netherlands line N07, Netherlands line N14, Netherlands line N15, Netherlands line N16, Netherlands line N17, Netherlands line N22, Netherlands line N29 and Netherlands line N30.
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF228474 mRNA. Translation: AAF33851.1.
AM050231 Genomic DNA. Translation: CAJ18903.1.
AM050232 Genomic DNA. Translation: CAJ18904.1.
AM050233 Genomic DNA. Translation: CAJ18905.1.
AM050234 Genomic DNA. Translation: CAJ18906.1.
AM050235 Genomic DNA. Translation: CAJ18907.1.
AM050236 Genomic DNA. Translation: CAJ18908.1.
AM050237 Genomic DNA. Translation: CAJ18909.1.
AM050238 Genomic DNA. Translation: CAJ18910.1.
AM050239 Genomic DNA. Translation: CAJ18911.1.
AM050240 Genomic DNA. Translation: CAJ18912.1.
AM050241 Genomic DNA. Translation: CAJ18913.1.
AM050242 Genomic DNA. Translation: CAJ18914.1.
AE014296 Genomic DNA. Translation: AAF50349.1.

RefSeq

NP_523986.2. NM_079262.3.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3IE4	X-ray	1.45	A/B	26-132	[»]

ProteinModelPortal

Q9NHA8.

SMR

Q9NHA8. Positions 26-126, 170-490.

ModBase

Search...

Protein-protein interaction databases

MINT

MINT-321664.

Protein family/group databases

CAZy

CBM39. Carbohydrate-Binding Module Family 39.
GH16. Glycoside Hydrolase Family 16.

Proteomic databases

PRIDE

Q9NHA8.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblMetazoa

FBtr0076510; FBpp0076237; FBgn0040321.

GeneID

39020.

KEGG

dme:Dmel_CG5008.

Organism-specific databases

CTD

39020.

FlyBase

FBgn0040321. GNBP3.

Phylogenomic databases

eggNOG

inNOG06871.

GeneTree

EMGT00050000015300.

InParanoid

Q9NHA8.

OMA

GQANTHD.

OrthoDB

EOG444J1V.

PhylomeDB

Q9NHA8.

Gene expression databases

Bgee

Q9NHA8.

GermOnline

CG5008. Drosophila melanogaster.

Family and domain databases

InterPro

IPR008985. ConA-like_lec_gl.
IPR013320. ConA-like_subgrp.
IPR000757. Glyco_hydro_16.
[Graphical view]

Gene3D

G3DSA:2.60.120.200. ConA_like_subgrp. 1 hit.

Pfam

PF00722. Glyco_hydro_16. 1 hit.
[Graphical view]

SUPFAM

SSF49899. ConA_like_lec_gl. 1 hit.

ProtoNet

Search...

Other

NextBio

811505.

Entry information

Entry name

BGBP3_DROME

Accession

Primary (citable) accession number: Q9NHA8
Secondary accession number(s): A0ZX55 , A0ZX56, A0ZX59, A0ZX64, Q9VSR4

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2004
Last sequence update:	July 10, 2007
Last modified:	January 25, 2012
This is version 66 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project