Q9NH52 (MES4_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Histone-lysine N-methyltransferase mes-4
EC=2.1.1.43

Alternative name(s):
Maternal-effect sterile protein 4

Gene names

Name:	mes-4
ORF Names:	Y2H9A.1

Organism

Caenorhabditis elegans

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	898 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Histone methyltransferase. Dimethylates 'Lys-36' of histone H3, a specific tag for epigenetic transcriptional activation. Plays a central role in early development and is responsible for all H3 'Lys-36' dimethylation until about the 40-cell stage. Indirectly involved in the global inactivation of the X chromosomes in germline cells, possibly by excluding the mes-2-mes-3-mes-6 repressive Polycomb complex from the autosomes. Not related to transcription elongation. Ref.1 Ref.3
Catalytic activity	S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone]. Ref.3
Subcellular location	Nucleus. Chromosome. Note: Specifically associated with the autosomes and with the distal tip of chromosome X. Colocalizes with methylated 'Lys-4' of histone H3. Ref.3
Tissue specificity	In adults, it is predominantly expressed in the germline, and weakly expressed in intestinal cells.
Developmental stage	Expressed both maternally and zygotically. Expressed in all cells of early embryos. In late embryos and L1 larva, it is weakly expressed in somatic cells, while it is expressed at intermediate levels in the primordial germ cells Z2 and Z3. Ref.1
Disruption phenotype	Derepression of X-linked genes in the germline. Ref.1 Ref.3
Sequence similarities	Belongs to the histone-lysine methyltransferase family. SET2 subfamily. Contains 2 PHD-type zinc fingers. Contains 1 post-SET domain. Contains 1 SET domain.

Ontologies

Keywords
Cellular component	Chromosome Nucleus
Domain	Repeat Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Chromatin regulator Developmental protein Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	RNA interference Inferred from mutant phenotype. Source: WormBase determination of adult lifespan Inferred from mutant phenotype. Source: WormBase embryo development ending in birth or egg hatching Inferred from mutant phenotype. Source: WormBase germ-line sex determination Inferred from mutant phenotype Ref.1. Source: UniProtKB negative regulation of vulval development Inferred from mutant phenotype. Source: WormBase
Cellular component	X chromosome Inferred from direct assay. Source: WormBase autosome Inferred from direct assay. Source: WormBase nuclear chromosome Inferred from direct assay Ref.1. Source: UniProtKB
Molecular function	histone methyltransferase activity (H3-K36 specific) Inferred from mutant phenotype Ref.3. Source: WormBase zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 898	898	Histone-lysine N-methyltransferase mes-4		PRO_0000186083
Regions
	Domain	536 – 669	134	SET
	Domain	671 – 687	17	Post-SET
	Zinc finger	126 – 214	89	PHD-type 1
	Zinc finger	303 – 355	53	PHD-type 2
Experimental info
	Mutagenesis	877 – 898	22	Missing in BN87; maternal-effect mutation. Progeny defects in gonad proliferation. Germ cell degeneration. Abolishes histone methyltransferase activity. Ref.1 Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q9NH52 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 0FA6BC754FC9E295	FASTA	898	100,065
	10 20 30 40 50 60 MLPSSGDSSK ENCAPQDGIV EKEGPNKPFK KKNVKQRNAT PQGAGSETSS NAEDKQEEAP 70 80 90 100 110 120 ILTNAPKDII SMQIQETKAF LSEYEEFVDT DYPKTTFTHV PRKFLSVSPF TPIEADAEGE 130 140 150 160 170 180 FLPDADSKCV ICHEEKDKTG KSDLITCHGY INGEMDDGKR LLGNIPMFPC RSKFHASCMI 190 200 210 220 230 240 NYNSGGFHFQ YAARLECQAR LLCPLHCCNS CNLDHHKQSA YVGDIAECAL CLRAFHLTSC 250 260 270 280 290 300 YPSGGRDLNV SITIGGKVEK FEMIICPAHY LPGADVQFYN KHKKRKNAVT VVPKADVTMK 310 320 330 340 350 360 SHIKACCVIG CEKSSNSKTI MCKTCCRSFH SGCREVETLN GKPIPDDQCE SCVCGDPIPQ 370 380 390 400 410 420 NTLILAKWTD NSFWLALTLD WYKYPTGNRG NINFERLGYT VVQWLIPQEN DKEKQPLMSI 430 440 450 460 470 480 VPVSDIARLT KNYFSLAKNS TLRNLWEEKY EEQADTALKR CPYVCKTVFG RLRTSVYYKC 490 500 510 520 530 540 EPKLEEYHNN EVCNCEGADR CTKLSCQYLA DDYECPPSCS KKGVCHNRQV SMGIVSEKIK 550 560 570 580 590 600 LAATLCKGYG VFAKGQIEKD EYICEYVGEI IDKAEKKRRL DSVSISRDFQ ANHYMMELHK 610 620 630 640 650 660 GLTVDAARYG NISRYINHSC DPNAASFVTK VFVKKTKEGS LYDTRSYIRA IRTIDDGDEI 670 680 690 700 710 720 TFSYNMNNEE NLPDCECGAE NCMGTMGKAK REKPEVADSS EKAAKKNKSS KKKSVKNQNR 730 740 750 760 770 780 KSQEAGKNGT ASKKSEISPS KPSTSSASST SFVQQASWPI SQNKKNLKKN SNQPVADTGS 790 800 810 820 830 840 TLSTSTELNF HEKPQELLSP VSSRSRAASS STPRAQKSKS RRDDVESEAP PVKRATPSLQ 850 860 870 880 890 TIQETGKAIE FPATKSAITK ARALSTQSVP SPHTVEVKVR AISTRGRVQK ETKRFEPI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Regulation of the different chromatin states of autosomes and X chromosomes in the germ line of C. elegans." Fong Y., Bender L., Wang W., Strome S. Science 296:2235-2238(2002) [PubMed: 12077420] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION, DEVELOPMENTAL STAGE, FUNCTION, MUTAGENESIS OF 877-VAL--ILE-898, DISRUPTION PHENOTYPE.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[3]	"MES-4: an autosome-associated histone methyltransferase that participates in silencing the X chromosomes in the C. elegans germ line." Bender L.B., Suh J., Carroll C.R., Fong Y., Fingerman I.M., Briggs S.D., Cao R., Zhang Y., Reinke V., Strome S. Development 133:3907-3917(2006) [PubMed: 16968818] [Abstract] Cited for: FUNCTION, ENZYME ACTIVITY, SUBCELLULAR LOCATION, MUTAGENESIS OF 877-VAL--ILE-898, DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF233290 mRNA. Translation: AAF62516.1. AL021448 Genomic DNA. Translation: CAA16276.2.
PIR	T26577.
RefSeq	NP_506333.1. NM_073932.3.
UniGene	Cel.6195.
3D structure databases
ProteinModelPortal	Q9NH52.
SMR	Q9NH52. Positions 303-358, 453-687.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9NH52. 4 interactions.
MINT	MINT-1040434.
STRING	Q9NH52.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	Y2H9A.1.1; Y2H9A.1.1; Y2H9A.1. Y2H9A.1.2; Y2H9A.1.2; Y2H9A.1.
GeneID	179824.
KEGG	cel:Y2H9A.1.
NMPDR	fig|6239.3.peg.20284.
UCSC	Y2H9A.1. c. elegans.
Organism-specific databases
CTD	43351.
WormBase	Y2H9A.1; CE27781; WBGene00003222; mes-4.
Phylogenomic databases
eggNOG	meNOG06667.
GeneTree	EMGT00050000000655.
InParanoid	Q9NH52.
OMA	YAARLEC.
PhylomeDB	Q9NH52.
Gene expression databases
ArrayExpress	Q9NH52.
Family and domain databases
InterPro	IPR003616. Post-SET_dom. IPR001214. SET_dom. IPR001841. Znf_RING. [Graphical view]
KO	K07117.
Pfam	PF00856. SET. 1 hit. [Graphical view]
SMART	SM00508. PostSET. 1 hit. SM00184. RING. 2 hits. SM00317. SET. 1 hit. [Graphical view]
PROSITE	PS50868. POST_SET. 1 hit. PS50280. SET. 1 hit. PS01359. ZF_PHD_1. False negative. PS50016. ZF_PHD_2. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	907012.

Entry information

Entry name

MES4_CAEEL

Accession

Primary (citable) accession number: Q9NH52
Secondary accession number(s): Q9XXS4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 28, 2003
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project