Q9NGC3 (CEG1A_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Centaurin-gamma-1A

Gene names

Name:	cenG1A
ORF Names:	CG31811

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	995 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	GTPase-activating protein for the ADP ribosylation factor family Potential.
Sequence similarities	Belongs to the centaurin gamma-like family. Contains 1 ANK repeat. Contains 1 Arf-GAP domain. Contains 1 PH domain.
Sequence caution	The sequence AAL28369.1 differs from that shown. Reason: Erroneous translation. Wrong choice of frame. Isoform A: The sequence AAF66064.1 differs from that shown. Reason: a duplication of 79 residues inserted in position 130

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Domain	ANK repeat Zinc-finger
Ligand	GTP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	GTPase activation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	regulation of ARF GTPase activity Inferred from electronic annotation. Source: InterPro small GTPase mediated signal transduction Non-traceable author statement. Source: UniProtKB
Cellular component	nucleus Non-traceable author statement. Source: UniProtKB
Molecular function	ARF GTPase activator activity Inferred from electronic annotation. Source: InterPro GTP binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform B (identifier: Q9NGC3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform A (identifier: Q9NGC3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-68: Missing.
     69-125: AHPPQPLISN...SIRDHVVCIE → MLAVKNFFLP...VPDRSEKRKS

Isoform C (identifier: Q9NGC3-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-232: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 995	995	Centaurin-gamma-1A		PRO_0000074221
Regions
	Domain	453 – 682	230	PH
	Domain	679 – 822	144	Arf-GAP
	Repeat	866 – 898	33	ANK
	Nucleotide binding	149 – 156	8	GTP Potential
	Nucleotide binding	193 – 197	5	GTP Potential
	Nucleotide binding	249 – 252	4	GTP Potential
	Zinc finger	717 – 740	24	C4-type
	Region	137 – 340	204	Small GTPase-like
Natural variations
	Alternative sequence	1 – 232	232	Missing in isoform C.		VSP_000314
	Alternative sequence	1 – 68	68	Missing in isoform A.		VSP_000312
	Alternative sequence	69 – 125	57	AHPPQ…VVCIE → MLAVKNFFLPERAKAPETPQ RFSRMPEAFLRSIRRRSLRV KRAKSLVVPDRSEKRKS in isoform A.		VSP_000313
Experimental info
	Sequence conflict	334	1	N → D in AAO39542. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Isoform B [UniParc]. Last modified January 27, 2003. Version 2. Checksum: E2376662937C328F	FASTA	995	108,044
	10 20 30 40 50 60 MSNYHPHSHP HALSHPHPQQ VHNQLQNPHQ NQLPPPQRHN HAAPTAAASA SGAPSSSASA 70 80 90 100 110 120 SASASILVAH PPQPLISNSL AIRQEIQRFE SVHPSIYAIY ELIDLLPMAD AQIAQSIRDH 130 140 150 160 170 180 VVCIEDSFVN SQEWTISRSV PDLRLGIVGS LNSGKSALVH RYLTGSYMQE ESPEGGRFKK 190 200 210 220 230 240 EVFIDGQSYL LLIRDEGGAP EMQFAGWVDA VIFVFSLENE GSFNTVYNYY TKMAHFRNGQ 250 260 270 280 290 300 EIPMILVGTQ DAISERNPRV IDDTRARKLA SDLKRCSYYE TCATYGLNVE RVFQDACQKI 310 320 330 340 350 360 LSQRLPLPPQ VQPARPTTPQ GNRLGLAPYQ APTNGQRGQQ QLPLRMSADF AQAEQKLWSL 370 380 390 400 410 420 QAASSSTINE NNNITKYNPG AANSLQGDCS QVQLRDPRDL APPPGKELPT PTSTPTTSRK 430 440 450 460 470 480 SRRRSNLFIP SSSKKADKEK EPKSSELGSG RSIPIKQGYL YKRSSKSLNK EWKKKYVTLC 490 500 510 520 530 540 DDGRLTYHPS LHDYMDDVHG KEIPLQYVTV KVPGQKPRGS KSIITNSALT SSLMANGQRA 550 560 570 580 590 600 QNTLSDGIGC LTLAKDNQRK LSEKLSLLGA GSIAAGAGGE PLKSNSSQQT SGDEGIAMSN 610 620 630 640 650 660 SNSQTFIAGE VANAGNKLEA QTPNVKKRHR RMKSSSVKAN EADDNDGYEF YIVSLDSKQW 670 680 690 700 710 720 HFEAANSEER DEWVAAVEQE IFKSLQSIES SKTKQATSTD LAAMLAIRQR VPGNGFCVDC 730 740 750 760 770 780 GAPNPEWASL NLGVLMCIEC SGVHRNLGSH ISKVRSLGLD DWPSPHLSVM LAIGNSLANS 790 800 810 820 830 840 VWESNTRQRV KPTSQASRED KERWVRSKYE AKEFLTPLGN GSSAHPSPSP GQQLIEAVIR 850 860 870 880 890 900 ADIKSIVSIL ANCPSEVTNA NVSARDVRTP LLLACAIGNL AIAQLLIWNG ANIKHTDHEG 910 920 930 940 950 960 RTCLAYARAA QSLATAKSIK AAAAAQAGTT IPAPAPPTNG GIPAPQYNVE DTTALVELLE 970 980 990 GLGCPEAAPL TASGTLPRRR DTLGTPYEKS VSGVI
	Isoform A [UniParc]. Checksum: C21F862D0D7BFA89	FASTA	927	101,282
	10 20 30 40 50 60 MLAVKNFFLP ERAKAPETPQ RFSRMPEAFL RSIRRRSLRV KRAKSLVVPD RSEKRKSDSF 70 80 90 100 110 120 VNSQEWTISR SVPDLRLGIV GSLNSGKSAL VHRYLTGSYM QEESPEGGRF KKEVFIDGQS 130 140 150 160 170 180 YLLLIRDEGG APEMQFAGWV DAVIFVFSLE NEGSFNTVYN YYTKMAHFRN GQEIPMILVG 190 200 210 220 230 240 TQDAISERNP RVIDDTRARK LASDLKRCSY YETCATYGLN VERVFQDACQ KILSQRLPLP 250 260 270 280 290 300 PQVQPARPTT PQGNRLGLAP YQAPTNGQRG QQQLPLRMSA DFAQAEQKLW SLQAASSSTI 310 320 330 340 350 360 NENNNITKYN PGAANSLQGD CSQVQLRDPR DLAPPPGKEL PTPTSTPTTS RKSRRRSNLF 370 380 390 400 410 420 IPSSSKKADK EKEPKSSELG SGRSIPIKQG YLYKRSSKSL NKEWKKKYVT LCDDGRLTYH 430 440 450 460 470 480 PSLHDYMDDV HGKEIPLQYV TVKVPGQKPR GSKSIITNSA LTSSLMANGQ RAQNTLSDGI 490 500 510 520 530 540 GCLTLAKDNQ RKLSEKLSLL GAGSIAAGAG GEPLKSNSSQ QTSGDEGIAM SNSNSQTFIA 550 560 570 580 590 600 GEVANAGNKL EAQTPNVKKR HRRMKSSSVK ANEADDNDGY EFYIVSLDSK QWHFEAANSE 610 620 630 640 650 660 ERDEWVAAVE QEIFKSLQSI ESSKTKQATS TDLAAMLAIR QRVPGNGFCV DCGAPNPEWA 670 680 690 700 710 720 SLNLGVLMCI ECSGVHRNLG SHISKVRSLG LDDWPSPHLS VMLAIGNSLA NSVWESNTRQ 730 740 750 760 770 780 RVKPTSQASR EDKERWVRSK YEAKEFLTPL GNGSSAHPSP SPGQQLIEAV IRADIKSIVS 790 800 810 820 830 840 ILANCPSEVT NANVSARDVR TPLLLACAIG NLAIAQLLIW NGANIKHTDH EGRTCLAYAR 850 860 870 880 890 900 AAQSLATAKS IKAAAAAQAG TTIPAPAPPT NGGIPAPQYN VEDTTALVEL LEGLGCPEAA 910 920 PLTASGTLPR RRDTLGTPYE KSVSGVI
	Isoform C [UniParc]. Checksum: A108BCCB57496A48	FASTA	763	82,506
	10 20 30 40 50 60 MAHFRNGQEI PMILVGTQDA ISERNPRVID DTRARKLASD LKRCSYYETC ATYGLNVERV 70 80 90 100 110 120 FQDACQKILS QRLPLPPQVQ PARPTTPQGN RLGLAPYQAP TNGQRGQQQL PLRMSADFAQ 130 140 150 160 170 180 AEQKLWSLQA ASSSTINENN NITKYNPGAA NSLQGDCSQV QLRDPRDLAP PPGKELPTPT 190 200 210 220 230 240 STPTTSRKSR RRSNLFIPSS SKKADKEKEP KSSELGSGRS IPIKQGYLYK RSSKSLNKEW 250 260 270 280 290 300 KKKYVTLCDD GRLTYHPSLH DYMDDVHGKE IPLQYVTVKV PGQKPRGSKS IITNSALTSS 310 320 330 340 350 360 LMANGQRAQN TLSDGIGCLT LAKDNQRKLS EKLSLLGAGS IAAGAGGEPL KSNSSQQTSG 370 380 390 400 410 420 DEGIAMSNSN SQTFIAGEVA NAGNKLEAQT PNVKKRHRRM KSSSVKANEA DDNDGYEFYI 430 440 450 460 470 480 VSLDSKQWHF EAANSEERDE WVAAVEQEIF KSLQSIESSK TKQATSTDLA AMLAIRQRVP 490 500 510 520 530 540 GNGFCVDCGA PNPEWASLNL GVLMCIECSG VHRNLGSHIS KVRSLGLDDW PSPHLSVMLA 550 560 570 580 590 600 IGNSLANSVW ESNTRQRVKP TSQASREDKE RWVRSKYEAK EFLTPLGNGS SAHPSPSPGQ 610 620 630 640 650 660 QLIEAVIRAD IKSIVSILAN CPSEVTNANV SARDVRTPLL LACAIGNLAI AQLLIWNGAN 670 680 690 700 710 720 IKHTDHEGRT CLAYARAAQS LATAKSIKAA AAAQAGTTIP APAPPTNGGI PAPQYNVEDT 730 740 750 760 TALVELLEGL GCPEAAPLTA SGTLPRRRDT LGTPYEKSVS GVI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of novel ARF-GTPase activating protein (GAP)-like proteins in the Drosophila genome." Hendrick A.G., Harrington L.S. Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A).
[2]	"An exploration of the sequence of a 2.9-Mb region of the genome of Drosophila melanogaster: the Adh region." Ashburner M., Misra S., Roote J., Lewis S.E., Blazej R.G., Davis T., Doyle C., Galle R.F., George R.A., Harris N.L., Hartzell G., Harvey D.A., Hong L., Houston K.A., Hoskins R.A., Johnson G., Martin C., Moshrefi A.R. , Palazzolo M., Reese M.G., Spradling A.C., Tsang G., Wan K.H., Whitelaw K., Celniker S.E., Rubin G.M. Genetics 153:179-219(1999) [PubMed: 10471707] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING. Strain: Berkeley.
[5]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A). Strain: Berkeley. Tissue: Embryo and Ovary.
[6]	Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M., Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A., Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A. , Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S., Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E. Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B). Strain: Berkeley. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF254741 mRNA. Translation: AAF66064.1. Sequence problems. AE014134 Genomic DNA. Translation: AAF53343.2. AE014134 Genomic DNA. Translation: AAF53349.2. AE014134 Genomic DNA. Translation: AAF53350.2. AY122158 mRNA. Translation: AAM52670.1. AY060821 mRNA. Translation: AAL28369.1. Sequence problems. BT003538 mRNA. Translation: AAO39542.1.
RefSeq	NP_523562.2. NM_078838.2. NP_723849.1. NM_165067.1. NP_723850.1. NM_165068.1.
UniGene	Dm.1977.
3D structure databases
ProteinModelPortal	Q9NGC3.
SMR	Q9NGC3. Positions 141-304, 449-686, 704-967.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-21141N.
IntAct	Q9NGC3. 38 interactions.
MINT	MINT-300715.
STRING	Q9NGC3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0080546; FBpp0080123; FBgn0028509.
GeneID	34803.
KEGG	dme:Dmel_CG31811.
Organism-specific databases
CTD	34803.
FlyBase	FBgn0028509. cenG1A.
Phylogenomic databases
GeneTree	EMGT00050000002016.
InParanoid	Q9NGC3.
OMA	SALWHAN.
OrthoDB	EOG44MW6Z.
PhylomeDB	Q9NGC3.
Gene expression databases
ArrayExpress	Q9NGC3.
Bgee	Q9NGC3.
GermOnline	CG31811. Drosophila melanogaster.
Family and domain databases
InterPro	IPR002110. Ankyrin_rpt. IPR020683. Ankyrin_rpt-contain_dom. IPR001164. ArfGAP. IPR013684. MIRO-like. IPR011993. PH_type. IPR001849. Pleckstrin_homology. [Graphical view]
Gene3D	G3DSA:1.25.40.20. ANK. 1 hit. G3DSA:2.30.29.30. PH_type. 2 hits.
KO	K12491.
Pfam	PF12796. Ank_2. 1 hit. PF01412. ArfGap. 1 hit. PF08477. Miro. 1 hit. PF00169. PH. 1 hit. [Graphical view]
PRINTS	PR00405. REVINTRACTNG.
SMART	SM00248. ANK. 1 hit. SM00105. ArfGap. 1 hit. SM00233. PH. 1 hit. [Graphical view]
SUPFAM	SSF48403. ANK. 1 hit. SSF57863. ArfGAP. 1 hit.
PROSITE	PS50297. ANK_REP_REGION. 1 hit. PS50088. ANK_REPEAT. 1 hit. PS50115. ARFGAP. 1 hit. PS50003. PH_DOMAIN. 1 hit. PS51419. RAB. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	790290.

Entry information

Entry name

CEG1A_DROME

Accession

Primary (citable) accession number: Q9NGC3
Secondary accession number(s): Q86P12 , Q8MR30, Q95SF3, Q9NKE5, Q9VJV4, Q9VJV5, Q9VJV7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 27, 2003
Last sequence update:	January 27, 2003
Last modified:	January 25, 2012
This is version 97 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project