Q9NG98 (TOP3A_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA topoisomerase 3-alpha
EC=5.99.1.2

Alternative name(s):
DNA topoisomerase III alpha

Gene names

Name:	Top3alpha
ORF Names:	CG10123

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	1250 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. Weakly relaxes negative supercoils and displays a distinct preference for binding single-stranded DNA.
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.
Sequence similarities	Belongs to the type IA topoisomerase family. Contains 1 Toprim domain.

Ontologies

Keywords
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro double-strand break repair via homologous recombination Inferred from mutant phenotype. Source: FlyBase
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1250	1250	DNA topoisomerase 3-alpha		PRO_0000145195
Regions
	Domain	27 – 171	145	Toprim
Sites
	Active site	356	1	O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Experimental info
	Sequence conflict	111	1	K → E in AAF71288. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9NG98 [UniParc]. Last modified August 15, 2003. Version 2. Checksum: 108E4AC18EA5677A	FASTA	1,250	136,137
	10 20 30 40 50 60 MKAVLTCFRP VVASHRRYCA NPGQAMKYLN VAEKNDAAKT IAGLLSNGAA QRREGYSVYN 70 80 90 100 110 120 KVFDFEAPVR GQNAKMVMTS VSGHMMQLAF QVSYKNWRTV DPRSLFDAPV KKGVGSDYEP 130 140 150 160 170 180 IKRTLEREVR GCQGLIIWTD CDREGENIGY EIIDVCRAIK PNISVYRATF SEITTVAVRR 190 200 210 220 230 240 ALQQLGQPDK RQSDAVDVRT ELDLRTGAAI TRFQTMRLQR LFPEKIADKL ISYGSCQIPT 250 260 270 280 290 300 LGFVAERYKE IEAFVSEPFW KIKVLHTIDD LTVEFNWARN RLFDKEACEN YLLLCLAEPD 310 320 330 340 350 360 PRALVESVTV KPKHKWRPTP LDTVEMEKLG SRKLKLSAKE TMTIAEKLYT KGFISYPRTE 370 380 390 400 410 420 TNQFSKEFAL APLVEMQTGH RDWGAFAQRV IEWGPNPRNG NKSDQAHPPI HPTKLAENLQ 430 440 450 460 470 480 GNEARVYELV VRHFLACVSK DAVGSETLVH IDIAGEKFTA NGLVIHERNY LDVYVYDKWS 490 500 510 520 530 540 AKQIHHYENG QRFEPTEVSL HEGATTAPPL LTEADLIALM EKHGIGTDAT HAEHINTIKE 550 560 570 580 590 600 RGYIGVLDKG FLVPGVIGMG LYEGYDAMEL ALAKPQLRAE FELDLKLICQ GQKDPKVVLT 610 620 630 640 650 660 EQIAKYKQAY QQITDKITAM DAKISARINE TPAANSAVQE GADGSAPSHG IIQSIFQCPK 670 680 690 700 710 720 CNEAPLALKP KKNQQGWYIG CNNFPDCKNA VWLPTECKDA SVLDECCPTC GDGYRMLKFR 730 740 750 760 770 780 LSTPYYRGVF GTPSGWYKTC LPCDNLFRTT FNINLDSVKK VGGIVGEVRG GGGGPGPGPG 790 800 810 820 830 840 GGGSGRGAGS GGWSSGPGGG GSGGGGGSGG WGSGTGGGGS GGWGSGTGGG GLGGGKGKKP 850 860 870 880 890 900 GGESKKSATK KPPNEPKPKK TKEPKAAPNK KTSSKSSGSI RSFFTSAAPT NSASNGLDEF 910 920 930 940 950 960 FDSNDGFEDA MLAAAESVES SSQPKTISMV PLDDDIAAAF AADDDAEFEA LVNGGTMPTE 970 980 990 1000 1010 1020 SNGDQQLDKS LSEWIKEQDK ADERPMLWGT RERASLGTAA PTPPPKPAAK RPRWDSVERD 1030 1040 1050 1060 1070 1080 STPPSSVPES ETVLCTGCQQ PARQNTVRKN GPNLGRLYYK CPKPDECNFF QWADEPPSSA 1090 1100 1110 1120 1130 1140 KSKNSTGSAP QSTTSWGSNR VVTLPSIQQS NSQRGQSSMR SNSSSTVTIT QTKTKQQERN 1150 1160 1170 1180 1190 1200 TATPGDGEEV MCNCGQLASQ LTVRKDGPNQ GRPFYACPTR EKSCGFFKWG DEDQNQGASS 1210 1220 1230 1240 1250 TSWGSANRNP PGRSQPTAIT SDGPKTRRCG LCRKEGHTRN KCPRKDEFDM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Drosophila melanogaster topoisomerase III alpha." Plank J.L., Reineke J.C., Wilson T.M., Hsieh T.-S. Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Embryo.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[4]	Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Rubin G.M., Celniker S.E. Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF255733 mRNA. Translation: AAF71288.1. AE014134 Genomic DNA. Translation: AAF53813.2. BT015282 mRNA. Translation: AAT94511.1.
RefSeq	NP_523602.2. NM_078878.2.
UniGene	Dm.2659.
3D structure databases
ProteinModelPortal	Q9NG98.
SMR	Q9NG98. Positions 29-696.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9NG98.
Proteomic databases
PRIDE	Q9NG98.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0081246; FBpp0080787; FBgn0040268.
GeneID	35236.
KEGG	dme:Dmel_CG10123.
NMPDR	fig|7227.3.peg.3008.
Organism-specific databases
CTD	35236.
FlyBase	FBgn0040268. Top3alpha.
Phylogenomic databases
eggNOG	inNOG06744.
GeneTree	EMGT00050000000311.
InParanoid	Q9NG98.
OMA	LVIHERN.
OrthoDB	EOG43TX9H.
PhylomeDB	Q9NG98.
Gene expression databases
Bgee	Q9NG98.
GermOnline	CG10123. Drosophila melanogaster.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR023406. Topo_IA_AS. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013826. Topo_IA_cen_sub3. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR013498. Topo_IA_Znf. IPR006171. Toprim_domain. IPR001878. Znf_CCHC. IPR010666. Znf_GRF. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:1.10.290.10. Topo_IA_cen_sub3. 1 hit.
KO	K03165.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 1 hit. PF06839. zf-GRF. 2 hits. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. SM00343. ZnF_C2HC. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	792526.

Entry information

Entry name

TOP3A_DROME

Accession

Primary (citable) accession number: Q9NG98
Secondary accession number(s): Q6AWG6, Q9VIV1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	August 15, 2003
Last modified:	January 25, 2012
This is version 93 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project