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Protein

DNA topoisomerase 3-alpha

Gene

Top3alpha

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Weakly relaxes negative supercoils and displays a distinct preference for binding single-stranded DNA.By similarity

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei356 – 3561O-(5'-phospho-DNA)-tyrosine intermediateBy similarity

GO - Molecular functioni

GO - Biological processi

  • developmental growth Source: FlyBase
  • DNA topological change Source: InterPro
  • double-strand break repair via homologous recombination Source: FlyBase
  • mitochondrial genome maintenance Source: FlyBase
  • tissue regeneration Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 3-alpha (EC:5.99.1.2)
Alternative name(s):
DNA topoisomerase III alpha
Gene namesi
Name:Top3alpha
ORF Names:CG10123
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0040268. Top3alpha.

Subcellular locationi

GO - Cellular componenti

  • chromosome Source: InterPro
  • mitochondrion Source: FlyBase
  • nucleus Source: FlyBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12501250DNA topoisomerase 3-alphaPRO_0000145195Add
BLAST

Proteomic databases

PaxDbiQ9NG98.
PRIDEiQ9NG98.

Expressioni

Gene expression databases

BgeeiQ9NG98.
GenevisibleiQ9NG98. DM.

Interactioni

Protein-protein interaction databases

IntActiQ9NG98. 4 interactions.
STRINGi7227.FBpp0080787.

Structurei

3D structure databases

ProteinModelPortaliQ9NG98.
SMRiQ9NG98. Positions 27-625.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini27 – 171145ToprimPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the type IA topoisomerase family.Curated
Contains 1 Toprim domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0550.
GeneTreeiENSGT00530000063430.
InParanoidiQ9NG98.
KOiK03165.
OMAiLVIHERN.
OrthoDBiEOG74R1Q0.
PhylomeDBiQ9NG98.

Family and domain databases

Gene3Di1.10.290.10. 1 hit.
1.10.460.10. 3 hits.
3.40.50.140. 1 hit.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013826. Topo_IA_cen_sub3.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR013498. Topo_IA_Znf.
IPR006171. Toprim_domain.
IPR001878. Znf_CCHC.
IPR010666. Znf_GRF.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 1 hit.
PfamiPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 1 hit.
PF06839. zf-GRF. 2 hits.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
SM00343. ZnF_C2HC. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9NG98-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKAVLTCFRP VVASHRRYCA NPGQAMKYLN VAEKNDAAKT IAGLLSNGAA
60 70 80 90 100
QRREGYSVYN KVFDFEAPVR GQNAKMVMTS VSGHMMQLAF QVSYKNWRTV
110 120 130 140 150
DPRSLFDAPV KKGVGSDYEP IKRTLEREVR GCQGLIIWTD CDREGENIGY
160 170 180 190 200
EIIDVCRAIK PNISVYRATF SEITTVAVRR ALQQLGQPDK RQSDAVDVRT
210 220 230 240 250
ELDLRTGAAI TRFQTMRLQR LFPEKIADKL ISYGSCQIPT LGFVAERYKE
260 270 280 290 300
IEAFVSEPFW KIKVLHTIDD LTVEFNWARN RLFDKEACEN YLLLCLAEPD
310 320 330 340 350
PRALVESVTV KPKHKWRPTP LDTVEMEKLG SRKLKLSAKE TMTIAEKLYT
360 370 380 390 400
KGFISYPRTE TNQFSKEFAL APLVEMQTGH RDWGAFAQRV IEWGPNPRNG
410 420 430 440 450
NKSDQAHPPI HPTKLAENLQ GNEARVYELV VRHFLACVSK DAVGSETLVH
460 470 480 490 500
IDIAGEKFTA NGLVIHERNY LDVYVYDKWS AKQIHHYENG QRFEPTEVSL
510 520 530 540 550
HEGATTAPPL LTEADLIALM EKHGIGTDAT HAEHINTIKE RGYIGVLDKG
560 570 580 590 600
FLVPGVIGMG LYEGYDAMEL ALAKPQLRAE FELDLKLICQ GQKDPKVVLT
610 620 630 640 650
EQIAKYKQAY QQITDKITAM DAKISARINE TPAANSAVQE GADGSAPSHG
660 670 680 690 700
IIQSIFQCPK CNEAPLALKP KKNQQGWYIG CNNFPDCKNA VWLPTECKDA
710 720 730 740 750
SVLDECCPTC GDGYRMLKFR LSTPYYRGVF GTPSGWYKTC LPCDNLFRTT
760 770 780 790 800
FNINLDSVKK VGGIVGEVRG GGGGPGPGPG GGGSGRGAGS GGWSSGPGGG
810 820 830 840 850
GSGGGGGSGG WGSGTGGGGS GGWGSGTGGG GLGGGKGKKP GGESKKSATK
860 870 880 890 900
KPPNEPKPKK TKEPKAAPNK KTSSKSSGSI RSFFTSAAPT NSASNGLDEF
910 920 930 940 950
FDSNDGFEDA MLAAAESVES SSQPKTISMV PLDDDIAAAF AADDDAEFEA
960 970 980 990 1000
LVNGGTMPTE SNGDQQLDKS LSEWIKEQDK ADERPMLWGT RERASLGTAA
1010 1020 1030 1040 1050
PTPPPKPAAK RPRWDSVERD STPPSSVPES ETVLCTGCQQ PARQNTVRKN
1060 1070 1080 1090 1100
GPNLGRLYYK CPKPDECNFF QWADEPPSSA KSKNSTGSAP QSTTSWGSNR
1110 1120 1130 1140 1150
VVTLPSIQQS NSQRGQSSMR SNSSSTVTIT QTKTKQQERN TATPGDGEEV
1160 1170 1180 1190 1200
MCNCGQLASQ LTVRKDGPNQ GRPFYACPTR EKSCGFFKWG DEDQNQGASS
1210 1220 1230 1240 1250
TSWGSANRNP PGRSQPTAIT SDGPKTRRCG LCRKEGHTRN KCPRKDEFDM
Length:1,250
Mass (Da):136,137
Last modified:August 15, 2003 - v2
Checksum:i108E4AC18EA5677A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti111 – 1111K → E in AAF71288 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF255733 mRNA. Translation: AAF71288.1.
AE014134 Genomic DNA. Translation: AAF53813.2.
BT015282 mRNA. Translation: AAT94511.1.
RefSeqiNP_523602.2. NM_078878.3.
UniGeneiDm.2659.

Genome annotation databases

EnsemblMetazoaiFBtr0081246; FBpp0080787; FBgn0040268.
GeneIDi35236.
KEGGidme:Dmel_CG10123.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF255733 mRNA. Translation: AAF71288.1.
AE014134 Genomic DNA. Translation: AAF53813.2.
BT015282 mRNA. Translation: AAT94511.1.
RefSeqiNP_523602.2. NM_078878.3.
UniGeneiDm.2659.

3D structure databases

ProteinModelPortaliQ9NG98.
SMRiQ9NG98. Positions 27-625.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9NG98. 4 interactions.
STRINGi7227.FBpp0080787.

Proteomic databases

PaxDbiQ9NG98.
PRIDEiQ9NG98.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0081246; FBpp0080787; FBgn0040268.
GeneIDi35236.
KEGGidme:Dmel_CG10123.

Organism-specific databases

CTDi35236.
FlyBaseiFBgn0040268. Top3alpha.

Phylogenomic databases

eggNOGiCOG0550.
GeneTreeiENSGT00530000063430.
InParanoidiQ9NG98.
KOiK03165.
OMAiLVIHERN.
OrthoDBiEOG74R1Q0.
PhylomeDBiQ9NG98.

Miscellaneous databases

GenomeRNAii35236.
NextBioi792526.
PROiQ9NG98.

Gene expression databases

BgeeiQ9NG98.
GenevisibleiQ9NG98. DM.

Family and domain databases

Gene3Di1.10.290.10. 1 hit.
1.10.460.10. 3 hits.
3.40.50.140. 1 hit.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013826. Topo_IA_cen_sub3.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR013498. Topo_IA_Znf.
IPR006171. Toprim_domain.
IPR001878. Znf_CCHC.
IPR010666. Znf_GRF.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 1 hit.
PfamiPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 1 hit.
PF06839. zf-GRF. 2 hits.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
SM00343. ZnF_C2HC. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Drosophila melanogaster topoisomerase III alpha."
    Plank J.L., Reineke J.C., Wilson T.M., Hsieh T.-S.
    Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Embryo.
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  4. Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Rubin G.M., Celniker S.E.
    Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
    Tissue: Embryo.

Entry informationi

Entry nameiTOP3A_DROME
AccessioniPrimary (citable) accession number: Q9NG98
Secondary accession number(s): Q6AWG6, Q9VIV1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: August 15, 2003
Last modified: June 24, 2015
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.