SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q9NG98

- TOP3A_DROME

UniProt

Q9NG98 - TOP3A_DROME

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
DNA topoisomerase 3-alpha
Gene
Top3alpha, CG10123
Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. Weakly relaxes negative supercoils and displays a distinct preference for binding single-stranded DNA.

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei356 – 3561O-(5'-phospho-DNA)-tyrosine intermediate By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. DNA topoisomerase activity Source: FlyBase
  4. DNA topoisomerase type I activity Source: UniProtKB-EC
  5. zinc ion binding Source: InterPro

GO - Biological processi

  1. DNA topological change Source: InterPro
  2. developmental growth Source: FlyBase
  3. double-strand break repair via homologous recombination Source: FlyBase
  4. mitochondrial genome maintenance Source: FlyBase
  5. tissue regeneration Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 3-alpha (EC:5.99.1.2)
Alternative name(s):
DNA topoisomerase III alpha
Gene namesi
Name:Top3alpha
ORF Names:CG10123
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0040268. Top3alpha.

Subcellular locationi

GO - Cellular componenti

  1. chromosome Source: InterPro
  2. mitochondrion Source: FlyBase
  3. nucleus Source: FlyBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12501250DNA topoisomerase 3-alpha
PRO_0000145195Add
BLAST

Proteomic databases

PaxDbiQ9NG98.
PRIDEiQ9NG98.

Expressioni

Gene expression databases

BgeeiQ9NG98.

Interactioni

Protein-protein interaction databases

STRINGi7227.FBpp0080787.

Structurei

3D structure databases

ProteinModelPortaliQ9NG98.
SMRiQ9NG98. Positions 27-625.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini27 – 171145Toprim
Add
BLAST

Sequence similaritiesi

Contains 1 Toprim domain.

Phylogenomic databases

eggNOGiCOG0550.
GeneTreeiENSGT00530000063430.
InParanoidiQ9NG98.
KOiK03165.
OMAiLVIHERN.
OrthoDBiEOG74R1Q0.
PhylomeDBiQ9NG98.

Family and domain databases

Gene3Di1.10.290.10. 1 hit.
1.10.460.10. 3 hits.
3.40.50.140. 1 hit.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013826. Topo_IA_cen_sub3.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR013498. Topo_IA_Znf.
IPR006171. Toprim_domain.
IPR001878. Znf_CCHC.
IPR010666. Znf_GRF.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 1 hit.
PfamiPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 1 hit.
PF06839. zf-GRF. 2 hits.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
SM00343. ZnF_C2HC. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9NG98-1 [UniParc]FASTAAdd to Basket

« Hide

MKAVLTCFRP VVASHRRYCA NPGQAMKYLN VAEKNDAAKT IAGLLSNGAA     50
QRREGYSVYN KVFDFEAPVR GQNAKMVMTS VSGHMMQLAF QVSYKNWRTV 100
DPRSLFDAPV KKGVGSDYEP IKRTLEREVR GCQGLIIWTD CDREGENIGY 150
EIIDVCRAIK PNISVYRATF SEITTVAVRR ALQQLGQPDK RQSDAVDVRT 200
ELDLRTGAAI TRFQTMRLQR LFPEKIADKL ISYGSCQIPT LGFVAERYKE 250
IEAFVSEPFW KIKVLHTIDD LTVEFNWARN RLFDKEACEN YLLLCLAEPD 300
PRALVESVTV KPKHKWRPTP LDTVEMEKLG SRKLKLSAKE TMTIAEKLYT 350
KGFISYPRTE TNQFSKEFAL APLVEMQTGH RDWGAFAQRV IEWGPNPRNG 400
NKSDQAHPPI HPTKLAENLQ GNEARVYELV VRHFLACVSK DAVGSETLVH 450
IDIAGEKFTA NGLVIHERNY LDVYVYDKWS AKQIHHYENG QRFEPTEVSL 500
HEGATTAPPL LTEADLIALM EKHGIGTDAT HAEHINTIKE RGYIGVLDKG 550
FLVPGVIGMG LYEGYDAMEL ALAKPQLRAE FELDLKLICQ GQKDPKVVLT 600
EQIAKYKQAY QQITDKITAM DAKISARINE TPAANSAVQE GADGSAPSHG 650
IIQSIFQCPK CNEAPLALKP KKNQQGWYIG CNNFPDCKNA VWLPTECKDA 700
SVLDECCPTC GDGYRMLKFR LSTPYYRGVF GTPSGWYKTC LPCDNLFRTT 750
FNINLDSVKK VGGIVGEVRG GGGGPGPGPG GGGSGRGAGS GGWSSGPGGG 800
GSGGGGGSGG WGSGTGGGGS GGWGSGTGGG GLGGGKGKKP GGESKKSATK 850
KPPNEPKPKK TKEPKAAPNK KTSSKSSGSI RSFFTSAAPT NSASNGLDEF 900
FDSNDGFEDA MLAAAESVES SSQPKTISMV PLDDDIAAAF AADDDAEFEA 950
LVNGGTMPTE SNGDQQLDKS LSEWIKEQDK ADERPMLWGT RERASLGTAA 1000
PTPPPKPAAK RPRWDSVERD STPPSSVPES ETVLCTGCQQ PARQNTVRKN 1050
GPNLGRLYYK CPKPDECNFF QWADEPPSSA KSKNSTGSAP QSTTSWGSNR 1100
VVTLPSIQQS NSQRGQSSMR SNSSSTVTIT QTKTKQQERN TATPGDGEEV 1150
MCNCGQLASQ LTVRKDGPNQ GRPFYACPTR EKSCGFFKWG DEDQNQGASS 1200
TSWGSANRNP PGRSQPTAIT SDGPKTRRCG LCRKEGHTRN KCPRKDEFDM 1250
Length:1,250
Mass (Da):136,137
Last modified:August 15, 2003 - v2
Checksum:i108E4AC18EA5677A
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti111 – 1111K → E in AAF71288. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF255733 mRNA. Translation: AAF71288.1.
AE014134 Genomic DNA. Translation: AAF53813.2.
BT015282 mRNA. Translation: AAT94511.1.
RefSeqiNP_523602.2. NM_078878.3.
UniGeneiDm.2659.

Genome annotation databases

EnsemblMetazoaiFBtr0081246; FBpp0080787; FBgn0040268.
GeneIDi35236.
KEGGidme:Dmel_CG10123.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF255733 mRNA. Translation: AAF71288.1 .
AE014134 Genomic DNA. Translation: AAF53813.2 .
BT015282 mRNA. Translation: AAT94511.1 .
RefSeqi NP_523602.2. NM_078878.3.
UniGenei Dm.2659.

3D structure databases

ProteinModelPortali Q9NG98.
SMRi Q9NG98. Positions 27-625.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 7227.FBpp0080787.

Proteomic databases

PaxDbi Q9NG98.
PRIDEi Q9NG98.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0081246 ; FBpp0080787 ; FBgn0040268 .
GeneIDi 35236.
KEGGi dme:Dmel_CG10123.

Organism-specific databases

CTDi 35236.
FlyBasei FBgn0040268. Top3alpha.

Phylogenomic databases

eggNOGi COG0550.
GeneTreei ENSGT00530000063430.
InParanoidi Q9NG98.
KOi K03165.
OMAi LVIHERN.
OrthoDBi EOG74R1Q0.
PhylomeDBi Q9NG98.

Miscellaneous databases

GenomeRNAii 35236.
NextBioi 792526.
PROi Q9NG98.

Gene expression databases

Bgeei Q9NG98.

Family and domain databases

Gene3Di 1.10.290.10. 1 hit.
1.10.460.10. 3 hits.
3.40.50.140. 1 hit.
InterProi IPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013826. Topo_IA_cen_sub3.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR013498. Topo_IA_Znf.
IPR006171. Toprim_domain.
IPR001878. Znf_CCHC.
IPR010666. Znf_GRF.
[Graphical view ]
PANTHERi PTHR11390. PTHR11390. 1 hit.
Pfami PF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 1 hit.
PF06839. zf-GRF. 2 hits.
[Graphical view ]
PRINTSi PR00417. PRTPISMRASEI.
SMARTi SM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
SM00343. ZnF_C2HC. 1 hit.
[Graphical view ]
SUPFAMi SSF56712. SSF56712. 1 hit.
PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Drosophila melanogaster topoisomerase III alpha."
    Plank J.L., Reineke J.C., Wilson T.M., Hsieh T.-S.
    Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Embryo.
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  4. Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Rubin G.M., Celniker S.E.
    Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
    Tissue: Embryo.

Entry informationi

Entry nameiTOP3A_DROME
AccessioniPrimary (citable) accession number: Q9NG98
Secondary accession number(s): Q6AWG6, Q9VIV1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: August 15, 2003
Last modified: July 9, 2014
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi