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Q9NG98

- TOP3A_DROME

UniProt

Q9NG98 - TOP3A_DROME

Protein

DNA topoisomerase 3-alpha

Gene

Top3alpha

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 2 (15 Aug 2003)
      Previous versions | rss
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    Functioni

    Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. Weakly relaxes negative supercoils and displays a distinct preference for binding single-stranded DNA.By similarity

    Catalytic activityi

    ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei356 – 3561O-(5'-phospho-DNA)-tyrosine intermediateBy similarity

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. DNA topoisomerase activity Source: FlyBase
    3. DNA topoisomerase type I activity Source: UniProtKB-EC
    4. zinc ion binding Source: InterPro

    GO - Biological processi

    1. developmental growth Source: FlyBase
    2. DNA topological change Source: InterPro
    3. double-strand break repair via homologous recombination Source: FlyBase
    4. mitochondrial genome maintenance Source: FlyBase
    5. tissue regeneration Source: FlyBase

    Keywords - Molecular functioni

    Isomerase, Topoisomerase

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA topoisomerase 3-alpha (EC:5.99.1.2)
    Alternative name(s):
    DNA topoisomerase III alpha
    Gene namesi
    Name:Top3alpha
    ORF Names:CG10123
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome 2L

    Organism-specific databases

    FlyBaseiFBgn0040268. Top3alpha.

    Subcellular locationi

    GO - Cellular componenti

    1. chromosome Source: InterPro
    2. mitochondrion Source: FlyBase
    3. nucleus Source: FlyBase

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 12501250DNA topoisomerase 3-alphaPRO_0000145195Add
    BLAST

    Proteomic databases

    PaxDbiQ9NG98.
    PRIDEiQ9NG98.

    Expressioni

    Gene expression databases

    BgeeiQ9NG98.

    Interactioni

    Protein-protein interaction databases

    STRINGi7227.FBpp0080787.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9NG98.
    SMRiQ9NG98. Positions 27-625.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini27 – 171145ToprimPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the type IA topoisomerase family.Curated
    Contains 1 Toprim domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0550.
    GeneTreeiENSGT00530000063430.
    InParanoidiQ9NG98.
    KOiK03165.
    OMAiLVIHERN.
    OrthoDBiEOG74R1Q0.
    PhylomeDBiQ9NG98.

    Family and domain databases

    Gene3Di1.10.290.10. 1 hit.
    1.10.460.10. 3 hits.
    3.40.50.140. 1 hit.
    InterProiIPR000380. Topo_IA.
    IPR003601. Topo_IA_2.
    IPR023406. Topo_IA_AS.
    IPR013497. Topo_IA_cen.
    IPR013824. Topo_IA_cen_sub1.
    IPR013826. Topo_IA_cen_sub3.
    IPR023405. Topo_IA_core_domain.
    IPR003602. Topo_IA_DNA-bd.
    IPR013498. Topo_IA_Znf.
    IPR006171. Toprim_domain.
    IPR001878. Znf_CCHC.
    IPR010666. Znf_GRF.
    [Graphical view]
    PANTHERiPTHR11390. PTHR11390. 1 hit.
    PfamiPF01131. Topoisom_bac. 1 hit.
    PF01751. Toprim. 1 hit.
    PF01396. zf-C4_Topoisom. 1 hit.
    PF06839. zf-GRF. 2 hits.
    [Graphical view]
    PRINTSiPR00417. PRTPISMRASEI.
    SMARTiSM00437. TOP1Ac. 1 hit.
    SM00436. TOP1Bc. 1 hit.
    SM00493. TOPRIM. 1 hit.
    SM00343. ZnF_C2HC. 1 hit.
    [Graphical view]
    SUPFAMiSSF56712. SSF56712. 1 hit.
    PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
    PS50880. TOPRIM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9NG98-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKAVLTCFRP VVASHRRYCA NPGQAMKYLN VAEKNDAAKT IAGLLSNGAA     50
    QRREGYSVYN KVFDFEAPVR GQNAKMVMTS VSGHMMQLAF QVSYKNWRTV 100
    DPRSLFDAPV KKGVGSDYEP IKRTLEREVR GCQGLIIWTD CDREGENIGY 150
    EIIDVCRAIK PNISVYRATF SEITTVAVRR ALQQLGQPDK RQSDAVDVRT 200
    ELDLRTGAAI TRFQTMRLQR LFPEKIADKL ISYGSCQIPT LGFVAERYKE 250
    IEAFVSEPFW KIKVLHTIDD LTVEFNWARN RLFDKEACEN YLLLCLAEPD 300
    PRALVESVTV KPKHKWRPTP LDTVEMEKLG SRKLKLSAKE TMTIAEKLYT 350
    KGFISYPRTE TNQFSKEFAL APLVEMQTGH RDWGAFAQRV IEWGPNPRNG 400
    NKSDQAHPPI HPTKLAENLQ GNEARVYELV VRHFLACVSK DAVGSETLVH 450
    IDIAGEKFTA NGLVIHERNY LDVYVYDKWS AKQIHHYENG QRFEPTEVSL 500
    HEGATTAPPL LTEADLIALM EKHGIGTDAT HAEHINTIKE RGYIGVLDKG 550
    FLVPGVIGMG LYEGYDAMEL ALAKPQLRAE FELDLKLICQ GQKDPKVVLT 600
    EQIAKYKQAY QQITDKITAM DAKISARINE TPAANSAVQE GADGSAPSHG 650
    IIQSIFQCPK CNEAPLALKP KKNQQGWYIG CNNFPDCKNA VWLPTECKDA 700
    SVLDECCPTC GDGYRMLKFR LSTPYYRGVF GTPSGWYKTC LPCDNLFRTT 750
    FNINLDSVKK VGGIVGEVRG GGGGPGPGPG GGGSGRGAGS GGWSSGPGGG 800
    GSGGGGGSGG WGSGTGGGGS GGWGSGTGGG GLGGGKGKKP GGESKKSATK 850
    KPPNEPKPKK TKEPKAAPNK KTSSKSSGSI RSFFTSAAPT NSASNGLDEF 900
    FDSNDGFEDA MLAAAESVES SSQPKTISMV PLDDDIAAAF AADDDAEFEA 950
    LVNGGTMPTE SNGDQQLDKS LSEWIKEQDK ADERPMLWGT RERASLGTAA 1000
    PTPPPKPAAK RPRWDSVERD STPPSSVPES ETVLCTGCQQ PARQNTVRKN 1050
    GPNLGRLYYK CPKPDECNFF QWADEPPSSA KSKNSTGSAP QSTTSWGSNR 1100
    VVTLPSIQQS NSQRGQSSMR SNSSSTVTIT QTKTKQQERN TATPGDGEEV 1150
    MCNCGQLASQ LTVRKDGPNQ GRPFYACPTR EKSCGFFKWG DEDQNQGASS 1200
    TSWGSANRNP PGRSQPTAIT SDGPKTRRCG LCRKEGHTRN KCPRKDEFDM 1250
    Length:1,250
    Mass (Da):136,137
    Last modified:August 15, 2003 - v2
    Checksum:i108E4AC18EA5677A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti111 – 1111K → E in AAF71288. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF255733 mRNA. Translation: AAF71288.1.
    AE014134 Genomic DNA. Translation: AAF53813.2.
    BT015282 mRNA. Translation: AAT94511.1.
    RefSeqiNP_523602.2. NM_078878.3.
    UniGeneiDm.2659.

    Genome annotation databases

    EnsemblMetazoaiFBtr0081246; FBpp0080787; FBgn0040268.
    GeneIDi35236.
    KEGGidme:Dmel_CG10123.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF255733 mRNA. Translation: AAF71288.1 .
    AE014134 Genomic DNA. Translation: AAF53813.2 .
    BT015282 mRNA. Translation: AAT94511.1 .
    RefSeqi NP_523602.2. NM_078878.3.
    UniGenei Dm.2659.

    3D structure databases

    ProteinModelPortali Q9NG98.
    SMRi Q9NG98. Positions 27-625.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 7227.FBpp0080787.

    Proteomic databases

    PaxDbi Q9NG98.
    PRIDEi Q9NG98.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0081246 ; FBpp0080787 ; FBgn0040268 .
    GeneIDi 35236.
    KEGGi dme:Dmel_CG10123.

    Organism-specific databases

    CTDi 35236.
    FlyBasei FBgn0040268. Top3alpha.

    Phylogenomic databases

    eggNOGi COG0550.
    GeneTreei ENSGT00530000063430.
    InParanoidi Q9NG98.
    KOi K03165.
    OMAi LVIHERN.
    OrthoDBi EOG74R1Q0.
    PhylomeDBi Q9NG98.

    Miscellaneous databases

    GenomeRNAii 35236.
    NextBioi 792526.
    PROi Q9NG98.

    Gene expression databases

    Bgeei Q9NG98.

    Family and domain databases

    Gene3Di 1.10.290.10. 1 hit.
    1.10.460.10. 3 hits.
    3.40.50.140. 1 hit.
    InterProi IPR000380. Topo_IA.
    IPR003601. Topo_IA_2.
    IPR023406. Topo_IA_AS.
    IPR013497. Topo_IA_cen.
    IPR013824. Topo_IA_cen_sub1.
    IPR013826. Topo_IA_cen_sub3.
    IPR023405. Topo_IA_core_domain.
    IPR003602. Topo_IA_DNA-bd.
    IPR013498. Topo_IA_Znf.
    IPR006171. Toprim_domain.
    IPR001878. Znf_CCHC.
    IPR010666. Znf_GRF.
    [Graphical view ]
    PANTHERi PTHR11390. PTHR11390. 1 hit.
    Pfami PF01131. Topoisom_bac. 1 hit.
    PF01751. Toprim. 1 hit.
    PF01396. zf-C4_Topoisom. 1 hit.
    PF06839. zf-GRF. 2 hits.
    [Graphical view ]
    PRINTSi PR00417. PRTPISMRASEI.
    SMARTi SM00437. TOP1Ac. 1 hit.
    SM00436. TOP1Bc. 1 hit.
    SM00493. TOPRIM. 1 hit.
    SM00343. ZnF_C2HC. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56712. SSF56712. 1 hit.
    PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
    PS50880. TOPRIM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Drosophila melanogaster topoisomerase III alpha."
      Plank J.L., Reineke J.C., Wilson T.M., Hsieh T.-S.
      Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Embryo.
    2. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley.
    3. Cited for: GENOME REANNOTATION.
      Strain: Berkeley.
    4. Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Rubin G.M., Celniker S.E.
      Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Berkeley.
      Tissue: Embryo.

    Entry informationi

    Entry nameiTOP3A_DROME
    AccessioniPrimary (citable) accession number: Q9NG98
    Secondary accession number(s): Q6AWG6, Q9VIV1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 2001
    Last sequence update: August 15, 2003
    Last modified: October 1, 2014
    This is version 112 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3