Q9NFT9 (HXK1_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hexokinase type 1
EC=2.7.1.1

Gene names

Name:	Hex-t1
Synonyms:	Hex
ORF Names:	CG33102

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	465 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + D-hexose = ADP + D-hexose 6-phosphate.
Pathway	Carbohydrate metabolism; hexose metabolism.
Sequence similarities	Belongs to the hexokinase family.

Ontologies

Keywords
Biological process	Glycolysis
Coding sequence diversity	Polymorphism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular glucose homeostasis Inferred from Biological aspect of Ancestor. Source: RefGenome glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytosol Inferred from Biological aspect of Ancestor. Source: RefGenome
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW fructokinase activity Inferred from Biological aspect of Ancestor. Source: RefGenome glucokinase activity Inferred from Biological aspect of Ancestor. Source: RefGenome mannokinase activity Inferred from Biological aspect of Ancestor. Source: RefGenome
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 465	465	Hexokinase type 1		PRO_0000197596
Regions
	Region	139 – 165	27	Glucose-binding Potential
Sites
	Binding site	88	1	ATP By similarity
Natural variations
	Natural variant	33	1	N → H in strain: HFL97e3_12 and ZIM(S)e3_24. Ref.1
	Natural variant	243	1	Y → F in strain: DPF96e3_3.0, DPF96e3_4.2, DPF96e3_23.1, DPF96e3_74.2, VT97e3_41, SC96e3_12.3, HFL97e3_8, HFL97e3_12, HFL97e3_15, ZIM(S)e3_24 and ZIM(S)e3_35. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9NFT9 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 67B611920D56B6DD	FASTA	465	52,260
	10 20 30 40 50 60 MANTFNPEED FPEVYKVCKL FNPSIDDLEK IKNAMDREIT MGLSRDHHDR STVPCHLSYV 70 80 90 100 110 120 QDLPTGRERG QFLALEMMPT NCRIMLVKFS SERDIYTSSK CVIMPHTVAA GRGTEVFTFL 130 140 150 160 170 180 ATSIANFVKE KKVDKDNLPL GIAFAFTLKK LALDVGILVS WTKEFGAQGA IGKDVVQLLR 190 200 210 220 230 240 DALAKFPEIS VDVMGIINVG AGSLLALCWA QPDTRIGLIM GSIANSCYVE RVERCETYEG 250 260 270 280 290 300 DEYRKLMIIN SDWAHFGDTG QLDFIRNEYD RQLDTESINP GTRIYEKFSG ALCMGELVRI 310 320 330 340 350 360 IVLRLMKSGA IFAEDRRDYI GIQWKLDMVS LIEIVSDPPG VYTKAQEVMD KFRIRHCKER 370 380 390 400 410 420 DLAALKYICD TVTNRAAMLV ASGVSCLIDR MRLPQISIAV DGGIYRLHPT FSTVLNKYTR 430 440 450 460 LLADPNYNFE FVITQDSCGV GAAIMAGMAH ANKYKTDAKL FTMDY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Contrasting molecular population genetics of four hexokinases in Drosophila melanogaster, D. simulans and D. yakuba." Duvernell D.D., Eanes W.F. Genetics 156:1191-1201(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS HIS-33 AND PHE-243. Strain: DPF96e3_23.1, DPF96e3_3.0, DPF96e3_4.2, DPF96e3_74.2, DPF96e3_84.3, HFL97e3_12, HFL97e3_13, HFL97e3_15, HFL97e3_16, HFL97e3_8, SC96e3_12.3, SC96e3_19.4, VT97e3_1, VT97e3_39, VT97e3_41, ZIM(H)e3_38.4, ZIM(H)e3_39, ZIM(S)e3_24 and ZIM(S)e3_35.
[2]	Deobagkar D.D., Kulkarni G.V., Deobagkar D.N. Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Oregon-K.
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[5]	Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Rubin G.M., Celniker S.E. Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Testis.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF257590 Genomic DNA. Translation: AAG22891.1. AF257591 Genomic DNA. Translation: AAG22893.1. AF257592 Genomic DNA. Translation: AAG22895.1. AF257593 Genomic DNA. Translation: AAG22897.1. AF257594 Genomic DNA. Translation: AAG22899.1. AF257595 Genomic DNA. Translation: AAG22901.1. AF257596 Genomic DNA. Translation: AAG22903.1. AF257597 Genomic DNA. Translation: AAG22905.1. AF257598 Genomic DNA. Translation: AAG22907.1. AF257599 Genomic DNA. Translation: AAG22909.1. AF257600 Genomic DNA. Translation: AAG22911.1. AF257601 Genomic DNA. Translation: AAG22913.1. AF257602 Genomic DNA. Translation: AAG22915.1. AF257603 Genomic DNA. Translation: AAG22917.1. AF257604 Genomic DNA. Translation: AAG22919.1. AF257605 Genomic DNA. Translation: AAG22921.1. AF257606 Genomic DNA. Translation: AAG22923.1. AF257607 Genomic DNA. Translation: AAG22925.1. AF257608 Genomic DNA. Translation: AAG22927.1. AJ271350 Genomic DNA. Translation: CAB72131.1. AE014297 Genomic DNA. Translation: AAF56591.2. BT015307 mRNA. Translation: AAT94535.1.
RefSeq	NP_788744.1. NM_176567.2.
UniGene	Dm.21469.
3D structure databases
ProteinModelPortal	Q9NFT9.
SMR	Q9NFT9. Positions 21-449.
ModBase	Search...
Protein-protein interaction databases
STRING	7227.FBpp0084382.
Proteomic databases
PRIDE	Q9NFT9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0085010; FBpp0084382; FBgn0042711.
GeneID	117364.
KEGG	dme:Dmel_CG33102.
UCSC	CG33102-RA. d. melanogaster.
Organism-specific databases
CTD	117364.
FlyBase	FBgn0042711. Hex-t1.
Phylogenomic databases
eggNOG	COG5026.
GeneTree	ENSGT00390000017159.
InParanoid	Q9NFT9.
KO	K00844.
OMA	PTNCRIM.
OrthoDB	EOG4TDZ1G.
PhylomeDB	Q9NFT9.
Enzyme and pathway databases
UniPathway	UPA00242.
Gene expression databases
Bgee	Q9NFT9.
GermOnline	CG33102. Drosophila melanogaster.
Family and domain databases
InterPro	IPR001312. Hexokinase. IPR022673. Hexokinase_C. IPR022672. Hexokinase_N. [Graphical view]
PANTHER	PTHR19443. PTHR19443. 1 hit.
Pfam	PF00349. Hexokinase_1. 1 hit. PF03727. Hexokinase_2. 1 hit. [Graphical view]
PRINTS	PR00475. HEXOKINASE.
ProtoNet	Search...
Other
GenomeRNAi	117364.
NextBio	841931.

Entry information

Entry name

HXK1_DROME

Accession

Primary (citable) accession number: Q9NFT9
Secondary accession number(s): Q6AWE1, Q9VBF1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project