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Protein

Hexokinase type 2

Gene

Hex-t2

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + D-hexose = ADP + D-hexose 6-phosphate.

Pathwayi: hexose metabolism

This protein is involved in the pathway hexose metabolism, which is part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the pathway hexose metabolism and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei128 – 1281ATP

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DME-170822. Regulation of Glucokinase by Glucokinase Regulatory Protein.
R-DME-70153. Glucose transport.
R-DME-70171. Glycolysis.
UniPathwayiUPA00242.

Names & Taxonomyi

Protein namesi
Recommended name:
Hexokinase type 2 (EC:2.7.1.1)
Gene namesi
Name:Hex-t2
Synonyms:Hex
ORF Names:CG32849
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0042710. Hex-t2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 486486Hexokinase type 2PRO_0000197597Add
BLAST

Proteomic databases

PaxDbiQ9NFT7.
PRIDEiQ9NFT7.

Expressioni

Gene expression databases

BgeeiQ9NFT7.
ExpressionAtlasiQ9NFT7. differential.
GenevisibleiQ9NFT7. DM.

Interactioni

Protein-protein interaction databases

IntActiQ9NFT7. 1 interaction.
STRINGi7227.FBpp0084383.

Structurei

3D structure databases

ProteinModelPortaliQ9NFT7.
SMRiQ9NFT7. Positions 51-479.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini36 – 477442HexokinasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni93 – 225133Hexokinase small subdomainPROSITE-ProRule annotationAdd
BLAST
Regioni167 – 19327Glucose-bindingSequence analysisAdd
BLAST
Regioni226 – 466241Hexokinase large subdomainPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the hexokinase family.PROSITE-ProRule annotationCurated
Contains 1 hexokinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1369. Eukaryota.
COG5026. LUCA.
GeneTreeiENSGT00390000017159.
InParanoidiQ9NFT7.
KOiK00844.
OMAiGEYRNCN.
OrthoDBiEOG7S21X5.
PhylomeDBiQ9NFT7.

Family and domain databases

InterProiIPR001312. Hexokinase.
IPR019807. Hexokinase_BS.
IPR022673. Hexokinase_C.
IPR022672. Hexokinase_N.
[Graphical view]
PANTHERiPTHR19443. PTHR19443. 1 hit.
PfamiPF00349. Hexokinase_1. 1 hit.
PF03727. Hexokinase_2. 1 hit.
[Graphical view]
PROSITEiPS00378. HEXOKINASE_1. 1 hit.
PS51748. HEXOKINASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9NFT7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRKSTRLLTH SLFGPVFKIL FHNKTVCGGC NRKMPSLVNT EIEAAVKGFL
60 70 80 90 100
IDQEKMTEVV ERMTKEIKMG LAKDTHARAV IKCFVSHVQD LPTGKERGKY
110 120 130 140 150
LALDLGGSNF RVLLVNLISN SDVETMSKGY NFPQTLMSGS GKALFDFLAE
160 170 180 190 200
CLSEFCHSHG LENESLALGF TFSFPLQQQG LSKGILVAWT KGFSCEGVVG
210 220 230 240 250
KNVVSLLQEA IDRRGDLKIN TVAILNDTVG TLMSCAFYHP NCRIGLIVGT
260 270 280 290 300
GSNACYVEKT VNAECFEGYQ TSPKPSMIIN CEWGAFGDNG VLEFVRTSYD
310 320 330 340 350
KAVDKVTPNP GKQTFEKCIS GMYMGELVRL VITDMIAKGF MFHGIISEKI
360 370 380 390 400
QERWSFKTAY ISDVESDAPG EYRNCNKVLS ELGILGCQEP DKEALRYICE
410 420 430 440 450
AVSSRSAKLC ACGLVTIINK MNINEVAIGI DGSVYRFHPK YHDMLQYHMK
460 470 480
KLLKPGVKFE LVVSEDGSGR GAALVAATAV QAKSKL
Length:486
Mass (Da):53,486
Last modified:June 21, 2004 - v4
Checksum:i79D011558E1E5ED6
GO

Sequence cautioni

The sequence AAG22892.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAG22894.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAG22896.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAG22898.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAG22900.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAG22902.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAG22904.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAG22906.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAG22908.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAG22910.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAG22912.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAG22914.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAG22916.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAG22918.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAG22920.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAG22922.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAG22924.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAG22926.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAG22928.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAL13623.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence CAB67701.1 differs from that shown. Reason: Erroneous gene model prediction. Was originally thought to correspond to two different genes.Curated
The sequence CAB72132.1 differs from that shown. Reason: Erroneous gene model prediction. Was originally thought to correspond to two different genes.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti167 – 1671A → P (PubMed:11063694).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti194 – 1941S → N in strain: HFL97e3_15. 1 Publication
Natural varianti252 – 2521S → T in strain: ZIM(S)e3_24. 1 Publication
Natural varianti380 – 3801S → N in strain: SC96e3_12.3 and ZIM(S)e3_35. 1 Publication
Natural varianti484 – 4841S → N in strain: DPF96e3_23.1, SC96e3_12.3, HFL97e3_8, HFL97e3_12, HFL97e3_16, ZIM(S)e3_24 and ZIM(S)e3_35. 1 Publication
Natural varianti486 – 4861L → I in strain: DPF96e3_23.1, SC96e3_12.3, HFL97e3_8, HFL97e3_12, HFL97e3_16 and ZIM(S)e3_24. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF257590 Genomic DNA. Translation: AAG22892.1. Different initiation.
AF257591 Genomic DNA. Translation: AAG22894.1. Different initiation.
AF257592 Genomic DNA. Translation: AAG22896.1. Different initiation.
AF257593 Genomic DNA. Translation: AAG22898.1. Different initiation.
AF257594 Genomic DNA. Translation: AAG22900.1. Different initiation.
AF257595 Genomic DNA. Translation: AAG22902.1. Different initiation.
AF257596 Genomic DNA. Translation: AAG22904.1. Different initiation.
AF257597 Genomic DNA. Translation: AAG22906.1. Different initiation.
AF257598 Genomic DNA. Translation: AAG22908.1. Different initiation.
AF257599 Genomic DNA. Translation: AAG22910.1. Different initiation.
AF257600 Genomic DNA. Translation: AAG22912.1. Different initiation.
AF257601 Genomic DNA. Translation: AAG22914.1. Different initiation.
AF257602 Genomic DNA. Translation: AAG22916.1. Different initiation.
AF257603 Genomic DNA. Translation: AAG22918.1. Different initiation.
AF257604 Genomic DNA. Translation: AAG22920.1. Different initiation.
AF257605 Genomic DNA. Translation: AAG22922.1. Different initiation.
AF257606 Genomic DNA. Translation: AAG22924.1. Different initiation.
AF257607 Genomic DNA. Translation: AAG22926.1. Different initiation.
AF257608 Genomic DNA. Translation: AAG22928.1. Different initiation.
AJ271350 Genomic DNA. Translation: CAB67701.1. Sequence problems.
AJ271350 Genomic DNA. Translation: CAB72132.1. Sequence problems.
AE014297 Genomic DNA. Translation: AAN14073.2.
AY058394 mRNA. Translation: AAL13623.1. Different initiation.
RefSeqiNP_733151.2. NM_170272.3.

Genome annotation databases

EnsemblMetazoaiFBtr0085011; FBpp0084383; FBgn0042710.
GeneIDi43191.
KEGGidme:Dmel_CG32849.
UCSCiCG32849-RA. d. melanogaster.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF257590 Genomic DNA. Translation: AAG22892.1. Different initiation.
AF257591 Genomic DNA. Translation: AAG22894.1. Different initiation.
AF257592 Genomic DNA. Translation: AAG22896.1. Different initiation.
AF257593 Genomic DNA. Translation: AAG22898.1. Different initiation.
AF257594 Genomic DNA. Translation: AAG22900.1. Different initiation.
AF257595 Genomic DNA. Translation: AAG22902.1. Different initiation.
AF257596 Genomic DNA. Translation: AAG22904.1. Different initiation.
AF257597 Genomic DNA. Translation: AAG22906.1. Different initiation.
AF257598 Genomic DNA. Translation: AAG22908.1. Different initiation.
AF257599 Genomic DNA. Translation: AAG22910.1. Different initiation.
AF257600 Genomic DNA. Translation: AAG22912.1. Different initiation.
AF257601 Genomic DNA. Translation: AAG22914.1. Different initiation.
AF257602 Genomic DNA. Translation: AAG22916.1. Different initiation.
AF257603 Genomic DNA. Translation: AAG22918.1. Different initiation.
AF257604 Genomic DNA. Translation: AAG22920.1. Different initiation.
AF257605 Genomic DNA. Translation: AAG22922.1. Different initiation.
AF257606 Genomic DNA. Translation: AAG22924.1. Different initiation.
AF257607 Genomic DNA. Translation: AAG22926.1. Different initiation.
AF257608 Genomic DNA. Translation: AAG22928.1. Different initiation.
AJ271350 Genomic DNA. Translation: CAB67701.1. Sequence problems.
AJ271350 Genomic DNA. Translation: CAB72132.1. Sequence problems.
AE014297 Genomic DNA. Translation: AAN14073.2.
AY058394 mRNA. Translation: AAL13623.1. Different initiation.
RefSeqiNP_733151.2. NM_170272.3.

3D structure databases

ProteinModelPortaliQ9NFT7.
SMRiQ9NFT7. Positions 51-479.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9NFT7. 1 interaction.
STRINGi7227.FBpp0084383.

Proteomic databases

PaxDbiQ9NFT7.
PRIDEiQ9NFT7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0085011; FBpp0084383; FBgn0042710.
GeneIDi43191.
KEGGidme:Dmel_CG32849.
UCSCiCG32849-RA. d. melanogaster.

Organism-specific databases

CTDi43191.
FlyBaseiFBgn0042710. Hex-t2.

Phylogenomic databases

eggNOGiKOG1369. Eukaryota.
COG5026. LUCA.
GeneTreeiENSGT00390000017159.
InParanoidiQ9NFT7.
KOiK00844.
OMAiGEYRNCN.
OrthoDBiEOG7S21X5.
PhylomeDBiQ9NFT7.

Enzyme and pathway databases

UniPathwayiUPA00242.
ReactomeiR-DME-170822. Regulation of Glucokinase by Glucokinase Regulatory Protein.
R-DME-70153. Glucose transport.
R-DME-70171. Glycolysis.

Miscellaneous databases

GenomeRNAii43191.
PROiQ9NFT7.

Gene expression databases

BgeeiQ9NFT7.
ExpressionAtlasiQ9NFT7. differential.
GenevisibleiQ9NFT7. DM.

Family and domain databases

InterProiIPR001312. Hexokinase.
IPR019807. Hexokinase_BS.
IPR022673. Hexokinase_C.
IPR022672. Hexokinase_N.
[Graphical view]
PANTHERiPTHR19443. PTHR19443. 1 hit.
PfamiPF00349. Hexokinase_1. 1 hit.
PF03727. Hexokinase_2. 1 hit.
[Graphical view]
PROSITEiPS00378. HEXOKINASE_1. 1 hit.
PS51748. HEXOKINASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Contrasting molecular population genetics of four hexokinases in Drosophila melanogaster, D. simulans and D. yakuba."
    Duvernell D.D., Eanes W.F.
    Genetics 156:1191-1201(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS ASN-194; THR-252; ASN-380; ASN-484 AND ILE-486.
    Strain: DPF96e3_23.1, DPF96e3_3.0, DPF96e3_4.2, DPF96e3_74.2, DPF96e3_84.3, HFL97e3_12, HFL97e3_13, HFL97e3_15, HFL97e3_16, HFL97e3_8, SC96e3_12.3, SC96e3_19.4, VT97e3_1, VT97e3_39, VT97e3_41, ZIM(H)e3_38.4, ZIM(H)e3_39, ZIM(S)e3_24 and ZIM(S)e3_35.
  2. Deobagkar D.D., Kulkarni G.V., Deobagkar D.N.
    Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Oregon-K.
  3. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  4. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  5. "A Drosophila full-length cDNA resource."
    Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E.
    Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-486.
    Strain: Berkeley.
    Tissue: Head.

Entry informationi

Entry nameiHXK2_DROME
AccessioniPrimary (citable) accession number: Q9NFT7
Secondary accession number(s): Q95U08, Q9NFT8, Q9VBF1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: June 21, 2004
Last modified: June 8, 2016
This is version 123 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.