Q9NEW6 (RSP3_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable splicing factor, arginine/serine-rich 3

Alternative name(s):
CeSF2
CeSF2/ASF

Gene names

Name:	rsp-3
ORF Names:	Y111B2A.18

Organism

Caenorhabditis elegans [Reference proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	258 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays an essential role in embryogenesis. Ref.1 Ref.3
Subunit structure	Interacts with spk-1. Ref.1
Subcellular location	Nucleus By similarity UniProtKB Q07955.
Tissue specificity	Predominantly coexpressed with spk-1 in adult hermaphrodite germlines. Ref.1
Post-translational modification	Directly phosphorylated by spk-1 in vitro on serine residues of the RS domain. Ref.1
Sequence similarities	Belongs to the splicing factor SR family. Contains 2 RRM (RNA recognition motif) domains.

Ontologies

Keywords
Biological process	mRNA processing mRNA splicing
Cellular component	Nucleus
Domain	Repeat
Ligand	RNA-binding
Molecular function	Developmental protein
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	embryo development ending in birth or egg hatching Inferred from mutant phenotype Ref.3. Source: UniProtKB mRNA splice site selection Inferred from sequence or structural similarity Ref.3. Source: WormBase
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	RNA binding Non-traceable author statement Ref.3. Source: UniProtKB nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 258	258	Probable splicing factor, arginine/serine-rich 3		PRO_0000081950
Regions
	Domain	9 – 83	75	RRM 1
	Domain	123 – 197	75	RRM 2
	Compositional bias	84 – 116	33	Gly-rich (hinge region)
	Compositional bias	199 – 256	58	Arg/Ser-rich (RS domain)
Experimental info
	Sequence conflict	90	1	G → S in AAG36874. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9NEW6 [UniParc]. Last modified October 1, 2002. Version 2. Checksum: 35ABA5DA89AEF315	FASTA	258	28,680
	10 20 30 40 50 60 MPRGGSEDQK VYVGNLPGDV REKEVEDIFH KYGRIKYVDI KSGRGPAFAF VEFEDHRDAE 70 80 90 100 110 120 DAVRARDGYE FDGRRIRVEF TRGVGPRGPG GRPLQDGGDH RGGDFRGGRG GGRGGGPQRR 130 140 150 160 170 180 TGYRVIVEGL PPTGSWQDLK DHMRDAGDVC YADVARDGTG VVEFTRYEDV KYAVRKLDDT 190 200 210 220 230 240 KFRSHEGETA YIRVREDNSS GGGSGGGGRD RSRSRSPRAE RRASPKYSPR RSRSRSRSRS 250 RSRSRSASRS PSRSPSPQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"SPK-1, a C. elegans SR protein kinase homologue, is essential for embryogenesis and required for germline development." Kuroyanagi H., Kimura T., Wada K., Hisamoto N., Matsumoto K., Hagiwara M. Mech. Dev. 99:51-64(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, PHOSPHORYLATION OF RS DOMAIN, TISSUE SPECIFICITY.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[3]	"Functional characterization of SR and SR-related genes in Caenorhabditis elegans." Longman D., Johnstone I.L., Caceres J.F. EMBO J. 19:1625-1637(2000) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION, FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF242767 mRNA. Translation: AAG36874.1. AL132904 Genomic DNA. Translation: CAC35847.2.
RefSeq	NP_499649.2. NM_067248.4.
3D structure databases
ProteinModelPortal	Q9NEW6.
SMR	Q9NEW6. Positions 4-93, 124-197.
ModBase	Search...
Proteomic databases
PaxDb	Q9NEW6.
PRIDE	Q9NEW6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	Y111B2A.18.1; Y111B2A.18.1; Y111B2A.18. Y111B2A.18.2; Y111B2A.18.2; Y111B2A.18. Y111B2A.18.3; Y111B2A.18.3; Y111B2A.18.
GeneID	176688.
KEGG	cel:CELE_Y111B2A.18.
UCSC	Y111B2A.18.1. c. elegans.
Organism-specific databases
CTD	176688.
WormBase	Y111B2A.18; CE31089; WBGene00004700; rsp-3.
Phylogenomic databases
eggNOG	COG0724.
GeneTree	ENSGT00700000104103.
HOGENOM	HOG000276234.
InParanoid	Q9NEW6.
KO	K12890.
OMA	MPRGGSE.
Family and domain databases
Gene3D	3.30.70.330. 2 hits.
InterPro	IPR012677. Nucleotide-bd_a/b_plait. IPR000504. RRM_dom. [Graphical view]
Pfam	PF00076. RRM_1. 2 hits. [Graphical view]
SMART	SM00360. RRM. 2 hits. [Graphical view]
PROSITE	PS50102. RRM. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	893608.

Entry information

Entry name

RSP3_CAEEL

Accession

Primary (citable) accession number: Q9NEW6
Secondary accession number(s): Q9GQI7

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 21, 2004
Last sequence update:	October 1, 2002
Last modified:	May 1, 2013
This is version 79 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project