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Protein

Serine/threonine-protein phosphatase 5

Gene

pph-5

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine/threonine-protein phosphatase (PubMed:23569206, PubMed:26593036). Dephosphorylates cdc-37 (PubMed:23569206). Probably by dephosphorylating separase sep-1, may be involved in sep-1-mediated exocytosis of cortical granules during meiotic anaphase and mitotic cytokinesis (PubMed:21878498).3 Publications

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.2 Publications

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 Mg2+ or Mn2+ cations per subunit.By similarity

Enzyme regulationi

Autoinhibited. In the autoinhibited state, the TPR domain interacts with the catalytic region and prevents substrate access to the catalytic pocket (By similarity). daf-21/Hsp90 probably releases the autoinhibition by the TPR repeat (PubMed:26593036, PubMed:23569206).By similarity2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi241Magnesium or manganese 1By similarity1
Metal bindingi243Magnesium or manganese 1By similarity1
Binding sitei243SubstrateBy similarity1
Metal bindingi270Magnesium or manganese 1By similarity1
Metal bindingi270Magnesium or manganese 2By similarity1
Binding sitei274SubstrateBy similarity1
Metal bindingi302Magnesium or manganese 2By similarity1
Active sitei303Proton donor/acceptorBy similarity1
Metal bindingi351Magnesium or manganese 2By similarity1
Binding sitei399SubstrateBy similarity1
Metal bindingi426Magnesium or manganese 2By similarity1
Binding sitei426SubstrateBy similarity1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • phosphoprotein phosphatase activity Source: UniProtKB

GO - Biological processi

  • negative regulation of exocytosis Source: WormBase
  • protein localization to organelle Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiR-CEL-5675221. Negative regulation of MAPK pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase 5By similarity (EC:3.1.3.162 Publications)
Gene namesi
Name:pph-5Imported
ORF Names:Y39B6A.2Imported
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome V

Organism-specific databases

WormBaseiY39B6A.2a; CE36619; WBGene00012665; pph-5.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: WormBase
  • mitotic spindle Source: WormBase
  • protein complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

Pathology & Biotechi

Disruption phenotypei

RNAi-mediated knockdown causes no visible phenotype.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi375P → E in av101; no obvious defects. Restores exocytosis of cortical granules, chromosome segregation, cytokinesis and polar body extrusion in a sep-1 (ax110) mutant background. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004378701 – 496Serine/threonine-protein phosphatase 5CuratedAdd BLAST496

Proteomic databases

EPDiQ9NES8.
PaxDbiQ9NES8.
PeptideAtlasiQ9NES8.

Expressioni

Tissue specificityi

Expressed in somatic and germline tissues.1 Publication

Gene expression databases

ExpressionAtlasiQ9NES8. differential.

Interactioni

Subunit structurei

Interacts (via catalytic region) with daf-21/Hsp90 (via TPR repeat-binding and central region); the interaction promotes pph-5 phosphatase activity.1 Publication

Protein-protein interaction databases

DIPiDIP-25900N.
IntActiQ9NES8. 3 interactors.
MINTiMINT-1080507.
STRINGi6239.Y39B6A.2a.2.

Structurei

3D structure databases

ProteinModelPortaliQ9NES8.
SMRiQ9NES8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati29 – 62TPR 1Sequence analysisAdd BLAST34
Repeati63 – 95TPR 2Sequence analysisAdd BLAST33
Repeati96 – 129TPR 3Sequence analysisAdd BLAST34

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni184 – 496CatalyticCuratedAdd BLAST313
Regioni302 – 303Substrate bindingBy similarity2

Sequence similaritiesi

Contains 3 TPR repeats.Sequence analysis

Keywords - Domaini

Repeat, TPR repeat

Phylogenomic databases

eggNOGiKOG0376. Eukaryota.
COG0639. LUCA.
GeneTreeiENSGT00530000063173.
HOGENOMiHOG000172698.
InParanoidiQ9NES8.
KOiK04460.
OMAiWMGRGPS.
PhylomeDBiQ9NES8.

Family and domain databases

Gene3Di1.25.40.10. 1 hit.
3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
IPR013235. PPP_dom.
IPR006186. Ser/Thr-sp_prot-phosphatase.
IPR011236. Ser/Thr_PPase_5.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR019734. TPR_repeat.
[Graphical view]
PANTHERiPTHR11668:SF21. PTHR11668:SF21. 2 hits.
PfamiPF00149. Metallophos. 1 hit.
PF08321. PPP5. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
SM00028. TPR. 2 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 1 hit.
SSF56300. SSF56300. 1 hit.
PROSITEiPS50005. TPR. 2 hits.
PS50293. TPR_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9NES8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAATITDDIV ATVLESIEEK SYEDEKEKAG MIKDEANQFF KDQVYDVAAD
60 70 80 90 100
LYSVAIEIHP TAVLYGNRAQ AYLKKELYGS ALEDADNAIA IDPSYVKGFY
110 120 130 140 150
RRATANMALG RFKKALTDYQ AVVKVCPNDK DARAKFDECS KIVRRQKFEA
160 170 180 190 200
AISTDHDKKT VAETLDINAM AIEDSYDGPR LEDKITKEFV LQLIKTFKNQ
210 220 230 240 250
QKLHKKYAFK MLLEFYNYVK SLPTMVEITV PTGKKFTICG DVHGQFYDLC
260 270 280 290 300
NIFEINGYPS ETNPYLFNGD FVDRGSFSVE TIFTMIGFKL LYPNHFFMSR
310 320 330 340 350
GNHESDVMNK MYGFEGEVKA KYTQQMCDMF TETFCWLPLC HLINEKIFVC
360 370 380 390 400
HGGLFKEDGV TLEDIRKTDR NRQPPDEGIM CDLLWSDPQP INGRSPSKRG
410 420 430 440 450
VGCQFGPDVT SKWCETNGIE YVVRSHEVKP EGYEMHHNGQ CFTVFSAPNY
460 470 480 490
CDQMNNKGAF ITITGDNLTP RFTPFDAVPH PKLPPMAYAN SLFGFN
Length:496
Mass (Da):56,462
Last modified:September 13, 2004 - v2
Checksum:i2C872FF1700384EE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX284605 Genomic DNA. Translation: CAC51076.2.
PIRiT45058.
RefSeqiNP_001256849.1. NM_001269920.1.
UniGeneiCel.1820.

Genome annotation databases

EnsemblMetazoaiY39B6A.2a; Y39B6A.2a; WBGene00012665.
GeneIDi180263.
KEGGicel:CELE_Y39B6A.2.
UCSCiY39B6A.2. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX284605 Genomic DNA. Translation: CAC51076.2.
PIRiT45058.
RefSeqiNP_001256849.1. NM_001269920.1.
UniGeneiCel.1820.

3D structure databases

ProteinModelPortaliQ9NES8.
SMRiQ9NES8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-25900N.
IntActiQ9NES8. 3 interactors.
MINTiMINT-1080507.
STRINGi6239.Y39B6A.2a.2.

Proteomic databases

EPDiQ9NES8.
PaxDbiQ9NES8.
PeptideAtlasiQ9NES8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiY39B6A.2a; Y39B6A.2a; WBGene00012665.
GeneIDi180263.
KEGGicel:CELE_Y39B6A.2.
UCSCiY39B6A.2. c. elegans.

Organism-specific databases

CTDi180263.
WormBaseiY39B6A.2a; CE36619; WBGene00012665; pph-5.

Phylogenomic databases

eggNOGiKOG0376. Eukaryota.
COG0639. LUCA.
GeneTreeiENSGT00530000063173.
HOGENOMiHOG000172698.
InParanoidiQ9NES8.
KOiK04460.
OMAiWMGRGPS.
PhylomeDBiQ9NES8.

Enzyme and pathway databases

ReactomeiR-CEL-5675221. Negative regulation of MAPK pathway.

Gene expression databases

ExpressionAtlasiQ9NES8. differential.

Family and domain databases

Gene3Di1.25.40.10. 1 hit.
3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
IPR013235. PPP_dom.
IPR006186. Ser/Thr-sp_prot-phosphatase.
IPR011236. Ser/Thr_PPase_5.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR019734. TPR_repeat.
[Graphical view]
PANTHERiPTHR11668:SF21. PTHR11668:SF21. 2 hits.
PfamiPF00149. Metallophos. 1 hit.
PF08321. PPP5. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
SM00028. TPR. 2 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 1 hit.
SSF56300. SSF56300. 1 hit.
PROSITEiPS50005. TPR. 2 hits.
PS50293. TPR_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPPP5_CAEEL
AccessioniPrimary (citable) accession number: Q9NES8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2016
Last sequence update: September 13, 2004
Last modified: November 30, 2016
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.