Q9NDH7 (GLY2_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase
EC=2.4.1.155

Alternative name(s):
GlcNAc-TV
Glycosylation-related protein 2
N-acetylglucosaminyltransferase gly-2

Gene names

Name:	gly-2
ORF Names:	C55B7.2

Organism

Caenorhabditis elegans

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	669 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the addition of N-acetylglucosamine (GlcNAc) in beta 1-6 linkage to the alpha-linked mannose of biantennary N-linked oligosaccharides. Ref.1
Catalytic activity	UDP-N-acetyl-D-glucosamine + 6-(2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R = UDP + 6-(2,6-bis(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein By similarity.
Tissue specificity	Expressed in a complex subset of neurons in larvae and in the spermathecal and pharyngeal-intestinal valves and certain vulval cells of adults. Ref.1
Developmental stage	In embryos, expressed from the late comma stage. Ref.1
Sequence similarities	Belongs to the glycosyltransferase 18 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Ligand	Metal-binding
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	embryo development ending in birth or egg hatching Inferred from mutant phenotype. Source: WormBase protein N-linked glycosylation Inferred from direct assay Ref.1. Source: WormBase
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell Golgi stack Traceable author statement Ref.1. Source: WormBase integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW nucleus Inferred from direct assay Ref.1. Source: WormBase
Molecular function	alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase activity Inferred from electronic annotation. Source: EC calmodulin binding Inferred from physical interaction. Source: WormBase metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 669	669	Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase		PRO_0000288613
Regions
	Topological domain	1 – 7	7	Cytoplasmic Potential
	Transmembrane	8 – 28	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	29 – 669	641	Lumenal Potential
Amino acid modifications
	Glycosylation	30	1	N-linked (GlcNAc...) Potential
	Glycosylation	412	1	N-linked (GlcNAc...) Potential
	Glycosylation	437	1	N-linked (GlcNAc...) Potential
	Glycosylation	626	1	N-linked (GlcNAc...) Potential
Experimental info
	Mutagenesis	116	1	L → R: Loss of function. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9NDH7 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 783CFC8B131769D9	FASTA	669	77,835
	10 20 30 40 50 60 MRRRHRCVAL LFIFSAFITP LGFFYYTISN ESKRYSEESE KNYGYQTLEF TESPEEISVD 70 80 90 100 110 120 FDKYSQSECS RFPSNVEIEY PECLNKMKWI KNGWKTHHCY IENHIDGSEC SFRYYLSQVE 130 140 150 160 170 180 NYCPPMEHHG KRKGLAKISP SIRRLLPIFE SIPHYMKTRI NRLWKKWKEG AHEVMQKYPK 190 200 210 220 230 240 SMIERRKLNV LVFIGFLANE QKLNMAKKSD HGGPLGELLQ WSDLLATLSV IGHHLEVSTN 250 260 270 280 290 300 KNTLRNIVWK YMSRGPCQYV NNFRQQLDII FTDIMGFNIL RQHHRQFLLS NRCRIRLLDS 310 320 330 340 350 360 FGTHAEFTTK TYFVQNKKSL SGPFSQRNPW GGHGLDLRQH WTFYPHSDDN TFLGFVVDTE 370 380 390 400 410 420 GIDKKNNQMI PSALVYGKEQ YMWRDAEKPI DVLKRIVTVH STVADLDLKD SNISSIFKKV 430 440 450 460 470 480 QNHGFLNSEE ISQLLDNITI FFGLGFPLEG PAPLEAMAHG AVFINAKFKE PKSRLNYKFL 490 500 510 520 530 540 AEKPTLRKWT SQNPYMEKIG EPHVITVDIF NELELEEAIK RAISLKPKHF VPFEFTPAGM 550 560 570 580 590 600 LHRVALLLEK QELCDKIAYS KRWPPIDQMK IFRTLNADDS CETICHSKQL LCEPSYFPII 610 620 630 640 650 660 NSSPLLRREN LCSSTTSDSS PFAPFNCTIQ QSAFLFSCAS SPPISFEINR LCPCRDYIPE QHAICKKCL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Caenorhabditis elegans gene, gly-2, can rescue the N-acetylglucosaminyltransferase V mutation of Lec4 cells." Warren C.E., Krizus A., Roy P.J., Culotti J.G., Dennis J.W. J. Biol. Chem. 277:22829-22838(2002) [PubMed: 11937505] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, MUTAGENESIS OF LEU-116. Strain: Bristol N2.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF154122 mRNA. Translation: AAF74523.1. AY037800 mRNA. Translation: AAK94765.1. AY037802 mRNA. Translation: AAK94767.1. FO080958 Genomic DNA. Translation: CCD68089.1.
RefSeq	NP_491874.1. NM_059473.5.
UniGene	Cel.17900.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q9NDH7.
Protein family/group databases
CAZy	GT18. Glycosyltransferase Family 18.
Proteomic databases
PRIDE	Q9NDH7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	C55B7.2; C55B7.2; C55B7.2.
GeneID	172360.
KEGG	cel:C55B7.2.
NMPDR	fig|6239.3.peg.1613.
UCSC	C55B7.2.1. c. elegans.
Organism-specific databases
CTD	172360.
WormBase	C55B7.2; CE27887; WBGene00001627; gly-2.
Phylogenomic databases
eggNOG	meNOG04379.
GeneTree	EMGT00050000020799.
HOGENOM	HBG505889.
InParanoid	Q9NDH7.
OMA	EQHAICK.
PhylomeDB	Q9NDH7.
Gene expression databases
ArrayExpress	Q9NDH7.
Family and domain databases
InterPro	IPR024144. Glyco_trans_18. [Graphical view]
KO	K00744.
PANTHER	PTHR15075. PTHR15075. 1 hit.
ProtoNet	Search...
Other
NextBio	875195.

Entry information

Entry name

GLY2_CAEEL

Accession

Primary (citable) accession number: Q9NDH7
Secondary accession number(s): Q962C0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	October 1, 2000
Last modified:	December 14, 2011
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project