ID   ACE4_CAEBR              Reviewed;         604 AA.
AC   Q9NDG8; A8WTF3; Q620F3;
DT   07-JUN-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   24-JAN-2024, entry version 107.
DE   RecName: Full=Acetylcholinesterase 4;
DE            Short=AChE 4;
DE            EC=3.1.1.7;
DE   Flags: Precursor;
GN   Name=ace-4; ORFNames=CBG02827;
OS   Caenorhabditis briggsae.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6238;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=AF16;
RA   Combes D., Grauso M., Fedon Y., Toutant J.-P., Arpagaus M.;
RT   "A fourth acetylcholinesterase gene in Caenorhabditis briggsae.";
RL   Submitted (JUN-1999) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AF16;
RX   PubMed=14624247; DOI=10.1371/journal.pbio.0000045;
RA   Stein L.D., Bao Z., Blasiar D., Blumenthal T., Brent M.R., Chen N.,
RA   Chinwalla A., Clarke L., Clee C., Coghlan A., Coulson A., D'Eustachio P.,
RA   Fitch D.H.A., Fulton L.A., Fulton R.E., Griffiths-Jones S., Harris T.W.,
RA   Hillier L.W., Kamath R., Kuwabara P.E., Mardis E.R., Marra M.A.,
RA   Miner T.L., Minx P., Mullikin J.C., Plumb R.W., Rogers J., Schein J.E.,
RA   Sohrmann M., Spieth J., Stajich J.E., Wei C., Willey D., Wilson R.K.,
RA   Durbin R.M., Waterston R.H.;
RT   "The genome sequence of Caenorhabditis briggsae: a platform for comparative
RT   genomics.";
RL   PLoS Biol. 1:166-192(2003).
CC   -!- FUNCTION: Rapidly hydrolyzes choline released into the synapse.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetylcholine + H2O = acetate + choline + H(+);
CC         Xref=Rhea:RHEA:17561, ChEBI:CHEBI:15354, ChEBI:CHEBI:15355,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30089; EC=3.1.1.7;
CC   -!- SUBCELLULAR LOCATION: Synapse {ECO:0000250}. Secreted {ECO:0000250}.
CC       Cell membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the type-B carboxylesterase/lipase family.
CC       {ECO:0000305}.
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DR   EMBL; AF159505; AAF80378.1; -; mRNA.
DR   EMBL; HE601438; CAP23764.3; -; Genomic_DNA.
DR   AlphaFoldDB; Q9NDG8; -.
DR   SMR; Q9NDG8; -.
DR   STRING; 6238.Q9NDG8; -.
DR   ESTHER; caebr-ACHE4; ACHE.
DR   MEROPS; S09.980; -.
DR   GlyCosmos; Q9NDG8; 6 sites, No reported glycans.
DR   EnsemblMetazoa; CBG02827a.1; CBG02827a.1; WBGene00025805.
DR   WormBase; CBG02827a; CBP37642; WBGene00025805; Cbr-ace-4.
DR   eggNOG; KOG4389; Eukaryota.
DR   HOGENOM; CLU_006586_13_0_1; -.
DR   InParanoid; Q9NDG8; -.
DR   OMA; ENTWAIN; -.
DR   OrthoDB; 4386at2759; -.
DR   Proteomes; UP000008549; Chromosome II.
DR   GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR   GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR   GO; GO:0045202; C:synapse; IEA:UniProtKB-SubCell.
DR   GO; GO:0003990; F:acetylcholinesterase activity; IEA:UniProtKB-EC.
DR   Gene3D; 3.40.50.1820; alpha/beta hydrolase; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR002018; CarbesteraseB.
DR   InterPro; IPR019826; Carboxylesterase_B_AS.
DR   InterPro; IPR019819; Carboxylesterase_B_CS.
DR   InterPro; IPR000997; Cholinesterase.
DR   PANTHER; PTHR43918; ACETYLCHOLINESTERASE; 1.
DR   PANTHER; PTHR43918:SF2; CARBOXYLIC ESTER HYDROLASE; 1.
DR   Pfam; PF00135; COesterase; 1.
DR   PRINTS; PR00878; CHOLNESTRASE.
DR   SUPFAM; SSF53474; alpha/beta-Hydrolases; 1.
DR   PROSITE; PS00122; CARBOXYLESTERASE_B_1; 1.
DR   PROSITE; PS00941; CARBOXYLESTERASE_B_2; 1.
PE   2: Evidence at transcript level;
KW   Cell membrane; Disulfide bond; Glycoprotein; Hydrolase; Membrane;
KW   Neurotransmitter degradation; Reference proteome; Secreted;
KW   Serine esterase; Signal; Synapse.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255"
FT   CHAIN           24..604
FT                   /note="Acetylcholinesterase 4"
FT                   /id="PRO_0000008611"
FT   ACT_SITE        219
FT                   /note="Acyl-ester intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10039"
FT   ACT_SITE        347
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        477
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   CARBOHYD        96
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        128
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        274
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        299
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        400
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        446
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        88..115
FT                   /evidence="ECO:0000250"
FT   DISULFID        273..284
FT                   /evidence="ECO:0000250"
FT   DISULFID        426..561
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   604 AA;  69952 MW;  73D4B6A653B325C4 CRC64;
     MKPKLVFLPF LIFITVFIEE SEAVHPVVLE TKLGDIRGNE FFFLSKKIRT FFGVPFAEPP
     VEEFRFRKPR EKKQWKKLFD ATKPANACFQ TRDNYNTSFW GSEMWNANTQ ISEDCLYLNI
     WAPADAYNLT VMVWFFGGGF YSGSPSLSIY DGRALAATQH VIVVNINYRL GPFGFLYLDH
     PDAPGNMGLL DQQLALHWIR QNIVSFGGNP DKVSVFGQSA GAASIVAHLI APGSRGLFKN
     AILQSGSLEN TWAINSPFRA KQKSEKLLEL VGCNKTTVEN SMSCLRLVSP EQLSLSTWNI
     SLTYLEFPFV IVSRDKHFFG HLDARAALRE GDFNRDVNLM IGMNKDEGNY WNIYQLPQFF
     DKAEPPELTR HQFDNLIDST FSIQPDIIRS AAKYIYSDPN CTDHGRKTRF YAGQMNQIVG
     DYFFSCDSLW LADQFFLFLQ ICSTPNGSLK NPPKVFVYYF TQSSSANPWP KWTGAMHGYE
     IEYVFGVPLS YSKIYKRREQ IFSRKIMQFW ASFAKNGTPR LRVLKNSEHW PEFNEHNNYR
     WMQLRSGSNI RPIKRRKETE CQFWRRVKDT EYTAYLTQEY SSSCHINSYR ILLFIPFFFI
     FSAF
//