Reviewed, UniProtKB/Swiss-Prot Q9NDG8 (ACE4_CAEBR)

Names and origin

Protein names

Recommended name:
    Acetylcholinesterase 4
      Short name=AChE 4
    EC=3.1.1.7

Gene names

Name:	ace-4
ORF Names:	CBG02827

Organism

Caenorhabditis briggsae [Complete proteome]

Taxonomic identifier

6238 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	604 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Rapidly hydrolyzes choline released into the synapse By similarity.
Catalytic activity	Acetylcholine + H2O = choline + acetate.
Subcellular location	Cell junction › synapse By similarity. Secreted By similarity. Cell membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the type-B carboxylesterase/lipase family.
Sequence caution	The sequence CAP23764.2 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Neurotransmitter degradation
Cellular component	Cell junction Cell membrane Membrane Secreted Synapse
Domain	Signal
Molecular function	Hydrolase Serine esterase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	neurotransmitter catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell synapse Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acetylcholinesterase activity Inferred from electronic annotation. Source: EC cholinesterase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 604	581	Acetylcholinesterase 4		PRO_0000008611
Sites
	Active site	219	1	Acyl-ester intermediate By similarity
	Active site	347	1	Charge relay system By similarity
	Active site	477	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	96	1	N-linked (GlcNAc...) Potential
	Glycosylation	128	1	N-linked (GlcNAc...) Potential
	Glycosylation	274	1	N-linked (GlcNAc...) Potential
	Glycosylation	299	1	N-linked (GlcNAc...) Potential
	Glycosylation	400	1	N-linked (GlcNAc...) Potential
	Glycosylation	446	1	N-linked (GlcNAc...) Potential
	Disulfide bond	88 ↔ 115		By similarity
	Disulfide bond	273 ↔ 284		By similarity
	Disulfide bond	426 ↔ 561		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9NDG8-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 73D4B6A653B325C4	FASTA	604	69,952
	10 20 30 40 50 60 MKPKLVFLPF LIFITVFIEE SEAVHPVVLE TKLGDIRGNE FFFLSKKIRT FFGVPFAEPP 70 80 90 100 110 120 VEEFRFRKPR EKKQWKKLFD ATKPANACFQ TRDNYNTSFW GSEMWNANTQ ISEDCLYLNI 130 140 150 160 170 180 WAPADAYNLT VMVWFFGGGF YSGSPSLSIY DGRALAATQH VIVVNINYRL GPFGFLYLDH 190 200 210 220 230 240 PDAPGNMGLL DQQLALHWIR QNIVSFGGNP DKVSVFGQSA GAASIVAHLI APGSRGLFKN 250 260 270 280 290 300 AILQSGSLEN TWAINSPFRA KQKSEKLLEL VGCNKTTVEN SMSCLRLVSP EQLSLSTWNI 310 320 330 340 350 360 SLTYLEFPFV IVSRDKHFFG HLDARAALRE GDFNRDVNLM IGMNKDEGNY WNIYQLPQFF 370 380 390 400 410 420 DKAEPPELTR HQFDNLIDST FSIQPDIIRS AAKYIYSDPN CTDHGRKTRF YAGQMNQIVG 430 440 450 460 470 480 DYFFSCDSLW LADQFFLFLQ ICSTPNGSLK NPPKVFVYYF TQSSSANPWP KWTGAMHGYE 490 500 510 520 530 540 IEYVFGVPLS YSKIYKRREQ IFSRKIMQFW ASFAKNGTPR LRVLKNSEHW PEFNEHNNYR 550 560 570 580 590 600 WMQLRSGSNI RPIKRRKETE CQFWRRVKDT EYTAYLTQEY SSSCHINSYR ILLFIPFFFI FSAF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A fourth acetylcholinesterase gene in Caenorhabditis briggsae." Combes D., Grauso M., Fedon Y., Toutant J.-P., Arpagaus M. Submitted (JUN-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: AF16.
[2]	"The genome sequence of Caenorhabditis briggsae: a platform for comparative genomics." Stein L.D., Bao Z., Blasiar D., Blumenthal T., Brent M.R., Chen N., Chinwalla A., Clarke L., Clee C., Coghlan A., Coulson A., D'Eustachio P., Fitch D.H.A., Fulton L.A., Fulton R.E., Griffiths-Jones S., Harris T.W., Hillier L.W. , Kamath R., Kuwabara P.E., Mardis E.R., Marra M.A., Miner T.L., Minx P., Mullikin J.C., Plumb R.W., Rogers J., Schein J.E., Sohrmann M., Spieth J., Stajich J.E., Wei C., Willey D., Wilson R.K., Durbin R.M., Waterston R.H. PLoS Biol. 1:166-192(2003) [PubMed: 14624247] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AF16.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF159505 mRNA. Translation: AAF80378.1. CAAC02000450 Genomic DNA. Translation: CAP23764.2. Sequence problems.
3D structure databases
SMR	Q9NDG8. Positions 27-567.
ModBase	Search...
Protein family/group databases
MEROPS	S09.980.
Organism-specific databases
WormBase	WBGene00025805. Cbr-ace-4.
Phylogenomic databases
HOGENOM	HBG716688.
PhylomeDB	Q9NDG8.
Enzyme and pathway databases
BRENDA	3.1.1.7. 261794.
Family and domain databases
InterPro	IPR002018. CarbesteraseB. IPR019826. Carboxylesterase_B_AS. IPR019819. Carboxylesterase_B_CS. IPR000997. Cholinesterase. [Graphical view]
PANTHER	PTHR11559. CarbesteraseB. 1 hit.
Pfam	PF00135. COesterase. 1 hit. [Graphical view]
PRINTS	PR00878. CHOLNESTRASE.
PROSITE	PS00122. CARBOXYLESTERASE_B_1. 1 hit. PS00941. CARBOXYLESTERASE_B_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACE4_CAEBR

Accession

Primary (citable) accession number: Q9NDG8
Secondary accession number(s): A8WTF3, Q620F3

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2004
Last sequence update:	October 1, 2000
Last modified:	February 9, 2010
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project