Q9NB71 (HIW_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
E3 ubiquitin-protein ligase highwire
EC=6.3.2.-

Alternative name(s):
Protein pam/highwire/rpm-1

Gene names

Name:	hiw
ORF Names:	CG32592

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	5233 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	E3 ubiquitin-protein ligases mediate ubiquitination and subsequent proteasomal degradation of target proteins. Has a role during synaptogenesis; negative regulator of synaptic growth. Ref.1
Pathway	Protein modification; protein ubiquitination.
Subcellular location	Nucleus By similarity UniProtKB O75592.
Tissue specificity	Nervous system specific expression. Stage 13 embryos show expression in the CNS at the longitudinal axon tracts around which the synaptic neuropil forms. Expression outside the CNS starts at stage 16 in presynaptic terminals at the periactive zone which surround the active zone. NMJ and CNS expression is also seen in third instar larvae (at protein level). Ref.1
Domain	The PHR domains are compact beta-sandwich folds composed of 11 antiparallel strands and decorated with conserved apical loops. They are likely to play a structural role and mediate interactions with substrates or partners By similarity.
Disruption phenotype	Flies display NMJ synapses that grow exuberantly and are expanded in both the number of boutons and in the extent and length of branches. Ref.1
Sequence similarities	Belongs to the highwire family. Contains 1 DOC domain. Contains 1 filamin repeat. Contains 6 RCC1 repeats. Contains 1 RING-type zinc finger.
Sequence caution	The sequence AAF48411.2 differs from that shown. Reason: Erroneous gene model prediction. The sequence AAM29381.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Transcription Transcription regulation Ubl conjugation pathway
Cellular component	Nucleus
Domain	Repeat Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Ligase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	BMP signaling pathway Inferred from physical interaction PubMed 15046722. Source: UniProtKB adult walking behavior Non-traceable author statement PubMed 12490252. Source: FlyBase autophagy Inferred from direct assay PubMed 19786572. Source: FlyBase locomotion Non-traceable author statement PubMed 12490252. Source: FlyBase negative regulation of BMP signaling pathway Inferred from genetic interaction PubMed 15046722. Source: UniProtKB negative regulation of synaptic growth at neuromuscular junction Inferred from mutant phenotype PubMed 15046722. Source: UniProtKB synapse organization Inferred from direct assay PubMed 19786572. Source: FlyBase transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasmic membrane-bounded vesicle Inferred from direct assay Ref.1. Source: FlyBase nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from direct assay Ref.1. Source: FlyBase
Molecular_function	ubiquitin-protein ligase activity Inferred from sequence or structural similarity. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 5233	5233	E3 ubiquitin-protein ligase highwire		PRO_0000055971
Regions
	Repeat	615 – 666	52	RCC1 1
	Repeat	669 – 724	56	RCC1 2
	Repeat	768 – 818	51	RCC1 3
	Repeat	931 – 983	53	RCC1 4
	Repeat	984 – 1033	50	RCC1 5
	Repeat	1035 – 1084	50	RCC1 6
	Repeat	2906 – 3000	95	Filamin
	Domain	4195 – 4374	180	DOC
	Zinc finger	4991 – 5042	52	RING-type; atypical
	Region	1436 – 1587	152	PHR domain 1 By similarity
	Region	2014 – 2169	156	PHR domain 2 By similarity
Amino acid modifications
	Modified residue	213	1	Phosphoserine Ref.5
	Modified residue	216	1	Phosphoserine Ref.5
Experimental info
	Sequence conflict	137	1	N → D in AAF76150. Ref.1
	Sequence conflict	604	1	C → R in AAF76150. Ref.1
	Sequence conflict	1620	1	F → S in AAF76150. Ref.1
	Sequence conflict	4652	1	G → E in AAF76150. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9NB71 [UniParc]. Last modified June 21, 2005. Version 2. Checksum: E5220FFDC91A58E7	FASTA	5,233	565,631
	10 20 30 40 50 60 MVGLLNPLKY GDHFYELYTN STRRKMQHDR KVLTKRKSRK DKSAAAMAAA AAAAVLEGNA 70 80 90 100 110 120 GAGGLVNPLD PPLVKEQLMI LPSFPVAHIE LDPNASKFAV FSAIRSTVLE AETRYSLFQE 130 140 150 160 170 180 GGAPSGQSGS NQKKWSNNVN SACCMMCAHS CMISNLLDCS CHAHMAMHGA VGPGNAGPGG 190 200 210 220 230 240 PVGVLVDGRI PAPVPVVVGG PGLPAEKRPR RDSANSDADS DTEEPTEREP VYATVPLIVG 250 260 270 280 290 300 AGLRSLFELI ADARHVHPLL CTKALKALLD VIQGQQPESF KLEPEELINP LYDLLLDLAT 310 320 330 340 350 360 MPAALNSATG AEANWSAMAC AALLGLCIAR GDTGKMLKAI AAMLMTPRQL SAQIVQLPVV 370 380 390 400 410 420 LATLQHTVIS AALNKPTRPD FHSHGVPHNS LIDEFPVKLP PLSTGAPITS PAMACDGVFV 430 440 450 460 470 480 YLLYGGTLLK IGTGFGGSYK GHIYAQNEDF SHERSAWLGY SGGQLYFRRT CRRSAGDQLQ 490 500 510 520 530 540 MVGLDTLAIK AMSPLSMLHM REGLNYVLFT DDDSLHAICS NRDDTLVVKK LNLYHNSYNI 550 560 570 580 590 600 DPPPFELPLQ LARKKFRTLG YAAFEDELLN QYQIQRIQSA HNSFEPKLPA PCRDADVDVM 610 620 630 640 650 660 GMACGKEFGL VRASNGRVYY YGKSAALGLK CVGRTPTLKL TELVISKAAN IVHVAVGHDG 670 680 690 700 710 720 IHALLVNDDG TVFFAGTARR GEDGDSSKNR RQPKAVKPKK MTKIDGHVVV HAACNNGTSA 730 740 750 760 770 780 FVTKTGKLIM YGKDTAHCDA MGFVSELLEQ HVTKVALGKA HCVALNAKGQ LFSFGLNNKG 790 800 810 820 830 840 QCGRIFNKLQ PVKDVPPFAS SSTAAACFAS LLPLDKRLKL DFSTLCDYDD HNLVQGQCRV 850 860 870 880 890 900 CVICRECTGY NVSCVSALNV PLDQRLAGSI CPCGHGDAGC AKCGLCAACI ALQDSDEAKT 910 920 930 940 950 960 ELKPPPSDVQ QRQQRSKTLI MRRKERKGEL ETGAAGGGAA TPTDLDKDPP RVAPLAPQLL 970 980 990 1000 1010 1020 QLTSSSPVVQ VACGLHHTVV LTLAGEVYTF GSNQYGQLGS GDLQPVSGPV RVQVPGAISQ 1030 1040 1050 1060 1070 1080 VAAGSNHTVL LTSKGMVYTF GNYQKGQLGR LPSDYGLKPP PQDDDSPVGA GSDGGAGGSP 1090 1100 1110 1120 1130 1140 TVAPVGMPGP ERSQSPANVQ PSGSKEMPPL LPVLTQRQKF LWNCSPGAVF GLGPCYGKKV 1150 1160 1170 1180 1190 1200 TWIGANGDQT FIKIDESLIT AQMLPKMHVV ANKKTILLIP SIPLSFHTLS INRRDGSCTA 1210 1220 1230 1240 1250 1260 HYRGQTNFVK LMQAQPEQQQ PEINSNLDVA VTPVADSPAH ASTSSLLAAL TGTATAGVPI 1270 1280 1290 1300 1310 1320 NEQMSRSMHE ARNQIFEEQL PEVGSSAAAA AVAAPGTPVS AGSVPRSRRG GKQGTSSPEP 1330 1340 1350 1360 1370 1380 IPSPPQLAFT MDPTYNVLWV FDGAARKLRC HNVVASDIND SDANAATYRS LLSPELSLPD 1390 1400 1410 1420 1430 1440 RVDSRVARSQ ASLNLLACLD ILTSAQDNIP GCFEQPLLKQ TQQTAETQAG EFQVVNRFDN 1450 1460 1470 1480 1490 1500 FGGGWGYSGH SVEAIRFSAD TDIVICGFGM FGGRGEYSCK LKLFDLGGDG GGYEKEGILI 1510 1520 1530 1540 1550 1560 SETKEVPYEC GARSKHHILL PKPVSAVAGR WYLVWARIAG PSSDCGSCGQ ASVTTEDQVV 1570 1580 1590 1600 1610 1620 FSFKSSKKAN NGTDVNSGQI PAILYRLVTQ DCKQTPAQMD ADPVQRISRA FANSVSRECF 1630 1640 1650 1660 1670 1680 ESLVVLLSWS WDCFKLQLRE ERDRSRPLQL QQSLQYLGYV IKSCLRLLRK YTIEIYPQRN 1690 1700 1710 1720 1730 1740 SSTSVATGGG SNAAHGSGVV TTAKSVQSKP NKDKNTPRVV GNAGVMAKYF GDPSTSVAPA 1750 1760 1770 1780 1790 1800 MISSASSGGA PSTSASAAVA PGSGTPVTRK TNMENIQLAE CIGNVRALLI GIFCDDIFKD 1810 1820 1830 1840 1850 1860 IATDEGYELS LEILDECHLS FVACFDAFYP TSSLKWNCLC DLLAQMDRGA LHSRLLSAIL 1870 1880 1890 1900 1910 1920 AGLCSPSVKL RATFSLLSAA GNERQSIISP SDNSGLPMLS STDAHPYPVL VEQMIYRTQQ 1930 1940 1950 1960 1970 1980 EKSDFLSNSW TFKDVLVRLL DIIASPIRSR IEAIYSRSLG SLGYPGGKDC VNQGLIDNCC 1990 2000 2010 2020 2030 2040 HLLARVLAEI VYQTAMGEYD KLFMPPRTLH STGARFARCD VSRTWNTGNF GPDAIAFAVD 2050 2060 2070 2080 2090 2100 RPGVAIAGAM VYSGSGSYDY QLELLYDNTA DLQPQHKWET LESVSGSYDQ DAVHNDLAEI 2110 2120 2130 2140 2150 2160 KFDHPVHIKE NARYALRLCS QGARTCSGDA GMPAVRGPCG AQFHFYACDL SFNGTTPARG 2170 2180 2190 2200 2210 2220 QLPCILYYST PMKQDGHSAS GRTGDGSNVA THLEDRIMLL GPHEVSTRDT ALQIAADITK 2230 2240 2250 2260 2270 2280 KCTELLILAR NAMAASCSPS DNSSNHTQTI DSEHNITPIE EHMDINWANN SRTAALPTAI 2290 2300 2310 2320 2330 2340 DPQLSTARDL GKRIESFSKG LMETLKFDKR STNPFEMEIE IGATEVEESA DLRNGQSQSV 2350 2360 2370 2380 2390 2400 SQSQSQSQSV PINGNERTAD FEFAEQSAQQ SMPQHLHSDS EEAPLEVAGM AAGGGVSVAD 2410 2420 2430 2440 2450 2460 GSGGVAGVGS QAAAVQLLEV FNLAASNMFH TLLPLVYAHI ANLACSDPKS SVQILGLIKE 2470 2480 2490 2500 2510 2520 ILPHIAALNQ LHVSKDQRQP EPAIFATQTS GSGNSNSSST TSNHYCVVES DHPYKSASIS 2530 2540 2550 2560 2570 2580 SYRVEFPPCV QWLTIEFDPQ CGTAQLEDYL LLSIPMRPAS QAPPVPHVDD YLEQADNNVN 2590 2600 2610 2620 2630 2640 GAGDRRRNTG GGIAGSGAAP NTHQRSASVQ LTMASCCRSP GCGNAPGSAA APSSMPLRSQ 2650 2660 2670 2680 2690 2700 DPNDREWIVV KKFNTASTWL HNVLILPGNC VEFSLETASL YAQDPHNNRY GFKCLVVGYD 2710 2720 2730 2740 2750 2760 NPTSINASNS CLIRLEQELA YLGGMCSANL MKKELNLPDD KDVEDMSGIE ETINTHHTLL 2770 2780 2790 2800 2810 2820 SKGFALSEPQ LTVHQALESY LPIGSQSNER QFLKDFISGA PGSSGARLAA WLQPESRLDP 2830 2840 2850 2860 2870 2880 NKCELNTITE PLRYGWPSQV TVTIRDQYGD AVLVPELKVE IKAIPTGSGP NGSATGTGTS 2890 2900 2910 2920 2930 2940 CTSVAEVSAP GPNLWMRRAS RDTWGWGGMA PPPRINYEPT VKDKMVFKAI TFMKPYANYS 2950 2960 2970 2980 2990 3000 FEELRYASPV QTRVTELLNA KDMEDGTFSV QWTPSSVGAY CLAVTIDGIP LEEVYRVDVK 3010 3020 3030 3040 3050 3060 EGILPPPTQR NSAQRRPQAP SKLRRFQARH SSGLRIRSHP TLQSEQVGVV RVGGVISFID 3070 3080 3090 3100 3110 3120 EIENDDGVWL RLSTESIRQH CTMGWYPTEA WCLQFNQHLA RMLLQPVTDK EVNPVRKGVG 3130 3140 3150 3160 3170 3180 AEEDVEEQPP VTPSASGEAS PEPEPDPSPV LSPAKTKPGR FLSGHQSTNP FLYPAKHADL 3190 3200 3210 3220 3230 3240 AEREAQVQEE REKEEEQVDD EDADDREPEQ EALPAVELLP AHIGSAIAGV VGGGAIKLQA 3250 3260 3270 3280 3290 3300 LQKWFKGDAV DGPQPLTPSH SPPLAGVSVR ELVRAMGGQD SPRGNGNRSQ QEQDPEFSLA 3310 3320 3330 3340 3350 3360 SMRRPNYSAS QTAALLSTPK HTPKRSAVVA SETSGLEDEL SLLQITTTTT GQGEQQSELQ 3370 3380 3390 3400 3410 3420 LATTSTASSA SKRNPMGPIK RAMPPSFAES IRAVFAALLW HEGVVHDAMA CASFLKFHPG 3430 3440 3450 3460 3470 3480 LPKEGATVVT RRGESGDPRL QLSREQKAQQ RHSVEVANAG NYLNIRPSTL ETLTKSGNCS 3490 3500 3510 3520 3530 3540 LHNRSKYRKN LLSGGGGAIN SGDDTAQKLQ ALPEMVSVLP PALRCLVYLW EQICSGCVQI 3550 3560 3570 3580 3590 3600 VQSNALEQRE PRLLSPGSRD LNGDADTEGK EGKNSDQASA GEKDLGRKCK RKKKDDGSWC 3610 3620 3630 3640 3650 3660 EICELFLPMP VTYHMRIAHP GCGKSAKGKG YNSVGIFCEG WAGNCGEGGK GASSWFLMCD 3670 3680 3690 3700 3710 3720 PCRDRYLASC RSANNINSAA RQLESSAAEG NELNLFGVKS TTLIANAEVY TTMRENATFL 3730 3740 3750 3760 3770 3780 LELCSSSSSA SGAAGSLAAT SSSSKRSPQQ MSVVAMPVVI EHQLGNSDLK PSTSRCSRMA 3790 3800 3810 3820 3830 3840 RLSGSKFCPG VGSGAFRKSF VGGPPTAPEN VWLAPESFAC LECLGTAGHE DLPYEMFGLG 3850 3860 3870 3880 3890 3900 PNSNDNGYDR PLSEISYESC EPNNYDMLSG SLAPGTTAAA SVGGGNLSKF HRSYSMGQGW 3910 3920 3930 3940 3950 3960 ASLAQHNHPP PHHPQQQHHQ QQQMNLQLQQ HQAPPVDGQP KVVYRRRNNS TSEGDGSLLI 3970 3980 3990 4000 4010 4020 CYPSEHLRRL VPQKLLASVS VMQTASGEGT GKDHATGTLG LDQSAGQNGG GNLLLTRPAM 4030 4040 4050 4060 4070 4080 AFITQKHELD RLRAAMRRSL RIAACRIYAL QALNWLLRSV TQGVCLHDLM WWFVSSLNPT 4090 4100 4110 4120 4130 4140 GGHQPVERGE EASEPALEHP VAYTQISGRF AHLITQSLHV FLQSVADLTL HLPLGSPLQR 4150 4160 4170 4180 4190 4200 VAIQCFGIRF RQADHQFLHS SHVFGNISKI LSKSDEQNDA MAVSTILKPD CDVEHNQVHS 4210 4220 4230 4240 4250 4260 VATGGSSGAG ARLLCYTDLA GMFEVTVSSR PAMAESLTDN STETFWESDE EDRNKCKIIE 4270 4280 4290 4300 4310 4320 LSLTKLNYAC RYLLVHIDNS RDIQNKVLNV VFYAGQSLGD TNIIKSADVD PKACSWISAK 4330 4340 4350 4360 4370 4380 ICDDSCTHFR LELHGPENTL RVRQIKLLGL PIGGAVGSDD SSDHKHQPHL RLSHASRIQQ 4390 4400 4410 4420 4430 4440 QICEAETLRV FRLITGQVFG KLISNVSSDL VPPDSAGIGP PSGGAASTSL LADSLDLREH 4450 4460 4470 4480 4490 4500 MVGILFSRSK LSHLQKQVIV HIVHAIRKEA QRAKEDWELA NLAHVLKQSP QQQTAPALAA 4510 4520 4530 4540 4550 4560 SASCESTPER SRAPDTYCFE MLSMVLALSG SVVGRSYLSQ QHGLLRDLLG LLHTGSDRVQ 4570 4580 4590 4600 4610 4620 RQVTALLRRI LPEITPESFA ELLGVQRLPP ADYSIAHQSA SDFDMSRLGL LDIFLAVIAK 4630 4640 4650 4660 4670 4680 SLQLQVKVKT TVASTGPSGS GGVSGSSSGN GGAVLKAGQQ EKTPAFVRLW SSLDLSVQQL 4690 4700 4710 4720 4730 4740 RSRPPTGEPG TTDPFQFDAL PPRKESKRNL NQRWFLNGVI STKQAESIIS LIRDLASGKL 4750 4760 4770 4780 4790 4800 SEKWSQITKA AIAESVLNLT RLEEIYRSPE HCTKTSTLWL ALASLCVLER DHVEKLSSGQ 4810 4820 4830 4840 4850 4860 WSKLCDTRPL CSNHDDGETA AIIQCETCGS LCGDCDRFLH LNRKTRSHKR TVCKEEEEAI 4870 4880 4890 4900 4910 4920 RVELHESCGR TKLFWLLALA DSKTLKAMVE FRDGSHTIIS GPQEAVGRCR FCGLTGNSGL 4930 4940 4950 4960 4970 4980 LEIGNVCADA QCQEYAANSC LKTKPCGHAC GGVTGERKCL PCLQHVCHTR ENELAEELRD 4990 5000 5010 5020 5030 5040 PKLTQDADDM CMICFVEALS CAPSIHLECG HVFHYHCCKA VLEKRWSGPR ITFGFSLCPI 5050 5060 5070 5080 5090 5100 CKADIQHPLL SDILEPINGL KQDVKRKALM RIKYEGVVKD TDSKNVNMTQ LAMDRYAYYV 5110 5120 5130 5140 5150 5160 CFKCQKAYYG GEARCDAEIG EKFDPEELVC GGCSDVARAQ MCPKHGTDFL EYKCRYCCSV 5170 5180 5190 5200 5210 5220 AVFFCFGTTH FCDTCHDDFQ RLTNIPKVKL PQCPAGPKAK QLLGDECPLH VMHPPTGEEF 5230 ALGCGVCRNA QTF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Highwire regulates synaptic growth in Drosophila." Wan H.I., DiAntonio A., Fetter R.D., Bergstrom K., Strauss R., Goodman C.S. Neuron 26:313-329(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE. Strain: Canton-S.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 4083-5233. Strain: Berkeley. Tissue: Larva and Pupae.
[5]	"Phosphoproteome analysis of Drosophila melanogaster embryos." Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P. J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-213 AND SER-216, MASS SPECTROMETRY. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF262977 mRNA. Translation: AAF76150.1. AE014298 Genomic DNA. Translation: AAF48411.2. Sequence problems. AY113376 mRNA. Translation: AAM29381.1. Different initiation.
RefSeq	NP_511159.3. NM_078604.3.
UniGene	Dm.3259.
3D structure databases
ProteinModelPortal	Q9NB71.
SMR	Q9NB71. Positions 597-783, 928-1064, 1431-1587, 2015-2170.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9NB71. 1 interaction.
STRING	7227.FBpp0073787.
Proteomic databases
PaxDb	Q9NB71.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0073970; FBpp0073787; FBgn0030600.
GeneID	32429.
KEGG	dme:Dmel_CG32592.
UCSC	CG32592-RA. d. melanogaster.
Organism-specific databases
CTD	32429.
FlyBase	FBgn0030600. hiw.
Phylogenomic databases
eggNOG	COG5184.
GeneTree	ENSGT00390000011403.
InParanoid	Q9NB71.
KO	K10693.
OMA	DAQCQEY.
OrthoDB	EOG4ZPC91.
PhylomeDB	Q9NB71.
Enzyme and pathway databases
UniPathway	UPA00143.
Gene expression databases
Bgee	Q9NB71.
GermOnline	CG32592. Drosophila melanogaster.
Family and domain databases
Gene3D	2.130.10.30. 4 hits. 2.60.40.10. 2 hits. 3.30.40.10. 1 hit.
InterPro	IPR004939. APC_su10/DOC_dom. IPR017868. Filamin/ABP280_repeat-like. IPR008979. Galactose-bd-like. IPR027047. Highwire/Pam/Rpm-1. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. IPR012983. PHR. IPR009091. RCC1/BLIP-II. IPR000408. Reg_chr_condens. IPR001841. Znf_RING. IPR013083. Znf_RING/FYVE/PHD. [Graphical view]
PANTHER	PTHR12846. PTHR12846. 1 hit.
Pfam	PF08005. PHR. 2 hits. PF00415. RCC1. 1 hit. [Graphical view]
PRINTS	PR00633. RCCNDNSATION.
SMART	SM00184. RING. 2 hits. [Graphical view]
SUPFAM	SSF49785. Gal_bind_like. 1 hit. SSF81296. Ig_E-set. 1 hit. SSF50985. RCC1/BLIP-II. 1 hit.
PROSITE	PS51284. DOC. 1 hit. PS50194. FILAMIN_REPEAT. 1 hit. PS00625. RCC1_1. False negative. PS00626. RCC1_2. 2 hits. PS50012. RCC1_3. 1 hit. PS00518. ZF_RING_1. False negative. PS50089. ZF_RING_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	hiw. drosophila.
GenomeRNAi	32429.
NextBio	778423.

Entry information

Entry name

HIW_DROME

Accession

Primary (citable) accession number: Q9NB71
Secondary accession number(s): Q8MZ36, Q9VXZ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	June 21, 2005
Last modified:	May 1, 2013
This is version 93 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project