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Protein

Patj homolog

Gene

Patj

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in cell polarity establishment. Probably participates in the assembly, positioning and maintenance of adherens junctions via its interaction with the SAC complex.1 Publication

GO - Molecular functioni

  • myosin II light chain binding Source: FlyBase
  • protein kinase C binding Source: FlyBase

GO - Biological processi

  • adherens junction maintenance Source: FlyBase
  • adherens junction organization Source: FlyBase
  • apical protein localization Source: FlyBase
  • cell-cell junction assembly Source: FlyBase
  • establishment of planar polarity Source: FlyBase
  • establishment or maintenance of apical/basal cell polarity Source: FlyBase
  • establishment or maintenance of cell polarity Source: FlyBase
  • establishment or maintenance of polarity of embryonic epithelium Source: FlyBase
  • establishment or maintenance of polarity of follicular epithelium Source: FlyBase
  • morphogenesis of an epithelium Source: FlyBase
  • morphogenesis of follicular epithelium Source: FlyBase
  • negative regulation of myosin-light-chain-phosphatase activity Source: FlyBase
  • photoreceptor cell maintenance Source: FlyBase
  • photoreceptor cell morphogenesis Source: FlyBase
  • pupal development Source: FlyBase
  • zonula adherens assembly Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Enzyme and pathway databases

SignaLinkiQ9NB04.

Names & Taxonomyi

Protein namesi
Recommended name:
Patj homolog
Gene namesi
Name:Patj
ORF Names:CG12021
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0067864. Patj.

Subcellular locationi

  • Membrane 2 Publications

  • Note: Membrane-associated. Localizes to the subapical domain of cells. Sdt is required for its localization. At mitotic cycles 13 and 14, during cellularization, it localizes at the leading edge of the invaginating membranes. It is then exclusively localized to the basal area of columnal epithelial cells. In the germline, it localizes at the membrane of the nurse cells during early and mid oogenesis. Later, it is found in the nuclear membrane in close association with actin filaments that connect the nuclei of the nurse cells with the plasma membrane.

GO - Cellular componenti

  • adherens junction Source: FlyBase
  • apical cortex Source: FlyBase
  • apical plasma membrane Source: FlyBase
  • apicolateral plasma membrane Source: FlyBase
  • cytoplasm Source: FlyBase
  • microtubule associated complex Source: FlyBase
  • nuclear envelope Source: FlyBase
  • plasma membrane Source: FlyBase
  • septate junction Source: FlyBase
  • subapical complex Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 871871Patj homologPRO_0000094591Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei682 – 6821Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9NB04.
PRIDEiQ9NB04.

PTM databases

iPTMnetiQ9NB04.

Expressioni

Tissue specificityi

Expressed in primary and some secondary epithelial cells such as ectodermal cells, salivary glands, foregut, hindgut and invaginating tracheal cells. Also expressed in specific cells of the peripheral nervous system. Expressed in the germline.2 Publications

Developmental stagei

Expressed both maternally and zygotically. Appears in mitotic cycle 11 when the furrows made by the imprint of the embryonic nuclei become apparent.1 Publication

Gene expression databases

BgeeiFBgn0067864.
GenevisibleiQ9NB04. DM.

Interactioni

Subunit structurei

Component of the SAC complex, a complex composed of crb, Patj and sdt. Interacts directly with nrx via its third and fourth PDZ domains. Interacts directly with par-6, possibly mediating a link between the SAC complex and the par-6 complex, which is composed of par-6, baz and aPKC.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
aPKCA1Z9X02EBI-442573,EBI-160861

GO - Molecular functioni

  • myosin II light chain binding Source: FlyBase
  • protein kinase C binding Source: FlyBase

Protein-protein interaction databases

BioGridi68832. 7 interactions.
IntActiQ9NB04. 6 interactions.
MINTiMINT-88981.
STRINGi7227.FBpp0072662.

Structurei

3D structure databases

ProteinModelPortaliQ9NB04.
SMRiQ9NB04. Positions 144-227, 254-401, 540-646, 719-811.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 6765L27PROSITE-ProRule annotationAdd
BLAST
Domaini149 – 22981PDZ 1PROSITE-ProRule annotationAdd
BLAST
Domaini318 – 40386PDZ 2PROSITE-ProRule annotationAdd
BLAST
Domaini559 – 64890PDZ 3PROSITE-ProRule annotationAdd
BLAST
Domaini728 – 81487PDZ 4PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi475 – 4817Poly-Thr
Compositional biasi699 – 7035Poly-Ala

Sequence similaritiesi

Belongs to the Patj family.Curated
Contains 1 L27 domain.PROSITE-ProRule annotation
Contains 4 PDZ (DHR) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3528. Eukaryota.
ENOG4110362. LUCA.
GeneTreeiENSGT00760000119017.
InParanoidiQ9NB04.
KOiK06095.
OMAiRMVCGRS.
OrthoDBiEOG091G0P7K.
PhylomeDBiQ9NB04.

Family and domain databases

Gene3Di2.30.42.10. 4 hits.
InterProiIPR004172. L27_dom.
IPR001478. PDZ.
[Graphical view]
PfamiPF00595. PDZ. 4 hits.
[Graphical view]
SMARTiSM00569. L27. 1 hit.
SM00228. PDZ. 4 hits.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 4 hits.
PROSITEiPS51022. L27. 1 hit.
PS50106. PDZ. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9NB04-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHLSADISSA LQQIEAVKKG IDESDDPKLQ MQTAESLSTI LGILQDPVFR
60 70 80 90 100
TIVHVQDSLS ELNAQLAQHP SMLPNDFDID VAGNLVLSLN GGEVMYDFDE
110 120 130 140 150
QRSSSHSHSA PGSPDKSGGV GEEPRPQSQN SKGAGVADLY ATDYAQIQAI
160 170 180 190 200
ELVNDGTGLG FGIIGARNSG VIVKTILPGG VADKDGRLRS GDHILQIGDV
210 220 230 240 250
NLHEMVSEQV AAVLRQSGTH VRLVVARPVE QSVPTPQYAL EPGTAVVPTR
260 270 280 290 300
VLVDPAELER YLISTGYPEI FGESSTASTP QTTTEDDRFV YRGETSMLID
310 320 330 340 350
PNIDLEELLA LPETEKLQVE LKKDANGLGI TIAGYVCEKE ELSGIFVKSV
360 370 380 390 400
SPGSAADLSG RIRVNDRIIE VDGQSLQGYS NHQAVELLKK SGQVVNLRLE
410 420 430 440 450
RYLRGPKFEQ LQQAIAANDK LPSSAPGTPS RAPMPTPVAT TSSATTTPSR
460 470 480 490 500
SITRELEEEA LPAPEAFMTT PPSVTTMTTT TLSSFGAGKQ LVAVRDSLDG
510 520 530 540 550
STKIIPTEVV PLADKTEAKN SGVITRHKYY TDPELSDDAE TEIIRKWQKI
560 570 580 590 600
VGSDVEVIVA QIKKFAVGGL GISLEGTVDV EGGREVRPHH YIRSILPDGP
610 620 630 640 650
VGVNGVLRSG DELLEVNGER LLGMNHLEVV AILKELPLDV RMVCGRNRNS
660 670 680 690 700
SLLPFSDDTL KKLSNNFENL LPATDRLVKA KSDGSLATAG SVADGDSVAA
710 720 730 740 750
AAASFSKLKS RSLEPLTGLA MWSSQPQIIE LVKGDRGLGF SILDYQDPLD
760 770 780 790 800
PNDTLIVIRS LVPGGVAQLD GRLIPGDRLL FVNSINLENA SLDQAVQALK
810 820 830 840 850
GASKGVVRIG VAKPLPMTDN SLKACSNAST TSEETLDAQP SPPALPTVAP
860 870
PAMPPSASMG AEPDLIPDWR N
Length:871
Mass (Da):92,852
Last modified:February 16, 2004 - v2
Checksum:iCE41E80AA2925B4D
GO

Sequence cautioni

The sequence AAD43031 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti349 – 3491S → N in AAF81829 (PubMed:11076972).Curated
Sequence conflicti359 – 3591S → N in AAF81829 (PubMed:11076972).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF103942 mRNA. Translation: AAD43031.1. Different initiation.
AF274350 mRNA. Translation: AAF81829.1.
AE014296 Genomic DNA. Translation: AAN11498.1.
AF132193 mRNA. Translation: AAD34781.1.
RefSeqiNP_477342.1. NM_057994.5.
NP_599144.1. NM_134317.4.
NP_728674.1. NM_167917.4.
UniGeneiDm.820.

Genome annotation databases

EnsemblMetazoaiFBtr0072779; FBpp0072662; FBgn0067864.
FBtr0072781; FBpp0072664; FBgn0067864.
FBtr0301625; FBpp0290839; FBgn0067864.
GeneIDi44100.
KEGGidme:Dmel_CG12021.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF103942 mRNA. Translation: AAD43031.1. Different initiation.
AF274350 mRNA. Translation: AAF81829.1.
AE014296 Genomic DNA. Translation: AAN11498.1.
AF132193 mRNA. Translation: AAD34781.1.
RefSeqiNP_477342.1. NM_057994.5.
NP_599144.1. NM_134317.4.
NP_728674.1. NM_167917.4.
UniGeneiDm.820.

3D structure databases

ProteinModelPortaliQ9NB04.
SMRiQ9NB04. Positions 144-227, 254-401, 540-646, 719-811.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi68832. 7 interactions.
IntActiQ9NB04. 6 interactions.
MINTiMINT-88981.
STRINGi7227.FBpp0072662.

PTM databases

iPTMnetiQ9NB04.

Proteomic databases

PaxDbiQ9NB04.
PRIDEiQ9NB04.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0072779; FBpp0072662; FBgn0067864.
FBtr0072781; FBpp0072664; FBgn0067864.
FBtr0301625; FBpp0290839; FBgn0067864.
GeneIDi44100.
KEGGidme:Dmel_CG12021.

Organism-specific databases

CTDi10207.
FlyBaseiFBgn0067864. Patj.

Phylogenomic databases

eggNOGiKOG3528. Eukaryota.
ENOG4110362. LUCA.
GeneTreeiENSGT00760000119017.
InParanoidiQ9NB04.
KOiK06095.
OMAiRMVCGRS.
OrthoDBiEOG091G0P7K.
PhylomeDBiQ9NB04.

Enzyme and pathway databases

SignaLinkiQ9NB04.

Miscellaneous databases

GenomeRNAii44100.
PROiQ9NB04.

Gene expression databases

BgeeiFBgn0067864.
GenevisibleiQ9NB04. DM.

Family and domain databases

Gene3Di2.30.42.10. 4 hits.
InterProiIPR004172. L27_dom.
IPR001478. PDZ.
[Graphical view]
PfamiPF00595. PDZ. 4 hits.
[Graphical view]
SMARTiSM00569. L27. 1 hit.
SM00228. PDZ. 4 hits.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 4 hits.
PROSITEiPS51022. L27. 1 hit.
PS50106. PDZ. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPATJ_DROME
AccessioniPrimary (citable) accession number: Q9NB04
Secondary accession number(s): Q9UA56, Q9XZ35
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 16, 2004
Last sequence update: February 16, 2004
Last modified: September 7, 2016
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Caution

Was originally thought to be the Disks lost (Dlt) protein. However, PubMed:14667407 showed that it is not the case and renamed it Patj. This drastically changes the first conclusions drawn about its essential function, since the mutant used contained defects for another protein, which is now called Dlt. If its association with proteins involved in cell polarization complexes is clear, Patj is not essential since its absence apparently does not lead to important defects.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.