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Protein

Patj homolog

Gene

Patj

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in cell polarity establishment. Probably participates in the assembly, positioning and maintenance of adherens junctions via its interaction with the SAC complex.1 Publication

Caution

Was originally thought to be the Disks lost (Dlt) protein. However, PubMed:14667407 showed that it is not the case and renamed it Patj. This drastically changes the first conclusions drawn about its essential function, since the mutant used contained defects for another protein, which is now called Dlt. If its association with proteins involved in cell polarization complexes is clear, Patj is not essential since its absence apparently does not lead to important defects.Curated

GO - Molecular functioni

  • myosin II light chain binding Source: FlyBase
  • protein kinase C binding Source: FlyBase

GO - Biological processi

  • adherens junction maintenance Source: FlyBase
  • adherens junction organization Source: FlyBase
  • apical protein localization Source: FlyBase
  • cell-cell junction assembly Source: FlyBase
  • establishment of planar polarity Source: FlyBase
  • establishment or maintenance of apical/basal cell polarity Source: FlyBase
  • establishment or maintenance of cell polarity Source: FlyBase
  • establishment or maintenance of polarity of embryonic epithelium Source: FlyBase
  • establishment or maintenance of polarity of follicular epithelium Source: FlyBase
  • morphogenesis of an epithelium Source: FlyBase
  • morphogenesis of follicular epithelium Source: FlyBase
  • negative regulation of myosin-light-chain-phosphatase activity Source: FlyBase
  • photoreceptor cell maintenance Source: FlyBase
  • photoreceptor cell morphogenesis Source: FlyBase
  • pupal development Source: FlyBase
  • zonula adherens assembly Source: FlyBase

Keywordsi

Molecular functionDevelopmental protein

Enzyme and pathway databases

SignaLinkiQ9NB04

Names & Taxonomyi

Protein namesi
Recommended name:
Patj homolog
Gene namesi
Name:Patj
ORF Names:CG12021
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0067864 Patj

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000945911 – 871Patj homologAdd BLAST871

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei682Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9NB04
PRIDEiQ9NB04

PTM databases

iPTMnetiQ9NB04

Expressioni

Tissue specificityi

Expressed in primary and some secondary epithelial cells such as ectodermal cells, salivary glands, foregut, hindgut and invaginating tracheal cells. Also expressed in specific cells of the peripheral nervous system. Expressed in the germline.2 Publications

Developmental stagei

Expressed both maternally and zygotically. Appears in mitotic cycle 11 when the furrows made by the imprint of the embryonic nuclei become apparent.1 Publication

Gene expression databases

BgeeiFBgn0067864
ExpressionAtlasiQ9NB04 baseline and differential
GenevisibleiQ9NB04 DM

Interactioni

Subunit structurei

Component of the SAC complex, a complex composed of crb, Patj and sdt. Interacts directly with nrx via its third and fourth PDZ domains. Interacts directly with par-6, possibly mediating a link between the SAC complex and the par-6 complex, which is composed of par-6, baz and aPKC.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
aPKCA1Z9X02EBI-442573,EBI-160861

GO - Molecular functioni

  • myosin II light chain binding Source: FlyBase
  • protein kinase C binding Source: FlyBase

Protein-protein interaction databases

BioGridi68832, 21 interactors
IntActiQ9NB04, 6 interactors
MINTiQ9NB04
STRINGi7227.FBpp0072662

Structurei

3D structure databases

ProteinModelPortaliQ9NB04
SMRiQ9NB04
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 67L27PROSITE-ProRule annotationAdd BLAST65
Domaini149 – 229PDZ 1PROSITE-ProRule annotationAdd BLAST81
Domaini318 – 403PDZ 2PROSITE-ProRule annotationAdd BLAST86
Domaini559 – 648PDZ 3PROSITE-ProRule annotationAdd BLAST90
Domaini728 – 814PDZ 4PROSITE-ProRule annotationAdd BLAST87

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi475 – 481Poly-Thr7
Compositional biasi699 – 703Poly-Ala5

Sequence similaritiesi

Belongs to the Patj family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3528 Eukaryota
ENOG4110362 LUCA
GeneTreeiENSGT00760000119017
InParanoidiQ9NB04
KOiK06095
OMAiRMVCGRS
OrthoDBiEOG091G0P7K
PhylomeDBiQ9NB04

Family and domain databases

InterProiView protein in InterPro
IPR004172 L27_dom
IPR001478 PDZ
IPR036034 PDZ_sf
PfamiView protein in Pfam
PF00595 PDZ, 4 hits
SMARTiView protein in SMART
SM00569 L27, 1 hit
SM00228 PDZ, 4 hits
SUPFAMiSSF50156 SSF50156, 4 hits
PROSITEiView protein in PROSITE
PS51022 L27, 1 hit
PS50106 PDZ, 4 hits

Sequencei

Sequence statusi: Complete.

Q9NB04-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHLSADISSA LQQIEAVKKG IDESDDPKLQ MQTAESLSTI LGILQDPVFR
60 70 80 90 100
TIVHVQDSLS ELNAQLAQHP SMLPNDFDID VAGNLVLSLN GGEVMYDFDE
110 120 130 140 150
QRSSSHSHSA PGSPDKSGGV GEEPRPQSQN SKGAGVADLY ATDYAQIQAI
160 170 180 190 200
ELVNDGTGLG FGIIGARNSG VIVKTILPGG VADKDGRLRS GDHILQIGDV
210 220 230 240 250
NLHEMVSEQV AAVLRQSGTH VRLVVARPVE QSVPTPQYAL EPGTAVVPTR
260 270 280 290 300
VLVDPAELER YLISTGYPEI FGESSTASTP QTTTEDDRFV YRGETSMLID
310 320 330 340 350
PNIDLEELLA LPETEKLQVE LKKDANGLGI TIAGYVCEKE ELSGIFVKSV
360 370 380 390 400
SPGSAADLSG RIRVNDRIIE VDGQSLQGYS NHQAVELLKK SGQVVNLRLE
410 420 430 440 450
RYLRGPKFEQ LQQAIAANDK LPSSAPGTPS RAPMPTPVAT TSSATTTPSR
460 470 480 490 500
SITRELEEEA LPAPEAFMTT PPSVTTMTTT TLSSFGAGKQ LVAVRDSLDG
510 520 530 540 550
STKIIPTEVV PLADKTEAKN SGVITRHKYY TDPELSDDAE TEIIRKWQKI
560 570 580 590 600
VGSDVEVIVA QIKKFAVGGL GISLEGTVDV EGGREVRPHH YIRSILPDGP
610 620 630 640 650
VGVNGVLRSG DELLEVNGER LLGMNHLEVV AILKELPLDV RMVCGRNRNS
660 670 680 690 700
SLLPFSDDTL KKLSNNFENL LPATDRLVKA KSDGSLATAG SVADGDSVAA
710 720 730 740 750
AAASFSKLKS RSLEPLTGLA MWSSQPQIIE LVKGDRGLGF SILDYQDPLD
760 770 780 790 800
PNDTLIVIRS LVPGGVAQLD GRLIPGDRLL FVNSINLENA SLDQAVQALK
810 820 830 840 850
GASKGVVRIG VAKPLPMTDN SLKACSNAST TSEETLDAQP SPPALPTVAP
860 870
PAMPPSASMG AEPDLIPDWR N
Length:871
Mass (Da):92,852
Last modified:February 16, 2004 - v2
Checksum:iCE41E80AA2925B4D
GO

Sequence cautioni

The sequence AAD43031 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti349S → N in AAF81829 (PubMed:11076972).Curated1
Sequence conflicti359S → N in AAF81829 (PubMed:11076972).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF103942 mRNA Translation: AAD43031.1 Different initiation.
AF274350 mRNA Translation: AAF81829.1
AE014296 Genomic DNA Translation: AAN11498.1
AF132193 mRNA Translation: AAD34781.1
RefSeqiNP_477342.1, NM_057994.5
NP_599144.1, NM_134317.4
NP_728674.1, NM_167917.4
UniGeneiDm.820

Genome annotation databases

EnsemblMetazoaiFBtr0072779; FBpp0072662; FBgn0067864
FBtr0072781; FBpp0072664; FBgn0067864
FBtr0301625; FBpp0290839; FBgn0067864
GeneIDi44100
KEGGidme:Dmel_CG12021

Similar proteinsi

Entry informationi

Entry nameiPATJ_DROME
AccessioniPrimary (citable) accession number: Q9NB04
Secondary accession number(s): Q9UA56, Q9XZ35
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 16, 2004
Last sequence update: February 16, 2004
Last modified: May 23, 2018
This is version 135 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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