Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q9NAV8 (Q9NAV8_9ANNE) Unreviewed, UniProtKB/TrEMBL

Last modified April 16, 2014. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein names
OrganismAmphitrite ornata EMBL AAF97245.1
Taxonomic identifier129555 [NCBI]
Taxonomic lineageEukaryotaMetazoaLophotrochozoaAnnelidaPolychaetaScolecidaTerebellidaAmphitrite

Protein attributes

Sequence length138 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Sequence similarities

Belongs to the globin family. RuleBase RU000356

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Region3 – 42Sulfate 1 binding PDB 4DWU PDB 1EWA PDB 2QFN PDB 4DWT PDB 3K3U PDB 3KUN PDB 3LB1 PDB 3LB2 PDB 3LB3 PDB 3LB4 PDB 1EW6 PDB 3MOU PDB 3MYN PDB 3MYM PDB 3KUO PDB 3O7N PDB 3OJ1 PDB 3OK5 PDB 3ORD PDB 4FH6 PDB 4FH7 PDB 4ILZ PDB 4GZG PDB 4HSX PDB 4JAC
Region3 – 42Sulfate 2 binding PDB 4ILZ
Region30 – 312Sulfate 2 binding PDB 4ILZ
Region30 – 312Sulfate 4 binding PDB 4DWU PDB 4DWT PDB 3K3U PDB 3MOU
Region32 – 354Sulfate 5 binding PDB 4DWU PDB 4DWT PDB 3K3U PDB 3MOU PDB 3O7N PDB 4FH6 PDB 4FH7
Region38 – 403Sulfate 8 binding PDB 3K3U
Region82 – 865Sulfate 11 binding
Region82 – 832Sulfate 12 binding PDB 4FH7
Region97 – 1004Sulfate 5 binding PDB 4DWU PDB 4DWT PDB 3K3U PDB 3MOU PDB 3O7N PDB 4FH6 PDB 4FH7
Region118 – 1192Sulfate 15 binding PDB 3KUO PDB 4FH7

Sites

Metal binding901Iron (heme axial ligand); via tele nitrogen PDB 4DWU PDB 1EWA PDB 2QFK PDB 3DR9 PDB 4DWT PDB 3K3U PDB 3KUN PDB 3LB1 PDB 3LB2 PDB 3LB3 PDB 3LB4 PDB 1EW6 PDB 3MOU PDB 3MYN PDB 3O7N PDB 4FH6 PDB 4FH7 PDB 4KMV PDB 4HSW PDB 4KMW PDB 4KN3 PDB 4JAC
Binding site31Sulfate 3; via amide nitrogen PDB 3O7N
Binding site331Sulfate 6 PDB 2QFN PDB 3LB4 PDB 3KUO PDB 3O7N PDB 4HSX PDB 4JAC
Binding site331Sulfate 7 PDB 2QFK PDB 2QFN PDB 3LB1 PDB 3LB2 PDB 3LB3 PDB 3MYN PDB 3MYM PDB 3ORD
Binding site411Sulfate 6; via amide nitrogen PDB 2QFN PDB 3LB4 PDB 3KUO PDB 3O7N PDB 4HSX PDB 4JAC
Binding site411Sulfate 7; via amide nitrogen PDB 2QFK PDB 2QFN PDB 3LB1 PDB 3LB2 PDB 3LB3 PDB 3MOU PDB 3MYN PDB 3MYM PDB 3ORD
Binding site481Sulfate 9 PDB 4FH7
Binding site561Azide PDB 4JAC
Binding site561Cyanide PDB 3KUN PDB 3MYN PDB 3MYM PDB 3KUO
Binding site561Heme (covalent) PDB 4GZG
Binding site561Sulfate 10 PDB 4DWU PDB 4DWT
Binding site881Sulfate 13; via amide nitrogen PDB 4FH7
Binding site991Sulfate 14 PDB 3LB3
Binding site1101Sulfate 15 PDB 3KUO PDB 4FH7
Binding site1101Sulfate 16 PDB 3K3U PDB 4HSX

Sequences

Sequence LengthMass (Da)Tools
Q9NAV8 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: E63F8C82A7CB90A9

FASTA13815,660
        10         20         30         40         50         60 
MGFKQDIATI RGDLRTYAQD IFLAFLNKYP DERRYFKNYV GKSDQELKSM AKFGDHTEKV 

        70         80         90        100        110        120 
FNLMMEVADR ATDCVPLASD ANTLVQMKQH SSLTTGNFEK LFVALVEYMR ASGQSFDSQS 

       130 
WDRFGKNLVS ALSSAGMK 

« Hide

References

[1]"The crystal structure and amino acid sequence of dehaloperoxidase from Amphitrite ornata indicate common ancestry with globins."
LaCount M.W., Zhang E., Chen Y.P., Han K., Whitton M.M., Lincoln D.E., Woodin S.A., Lebioda L.
J. Biol. Chem. 275:18712-18716(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.78 ANGSTROMS) OF 2-138 IN COMPLEX WITH HEME AND SULFATE.
[2]"Amphitrite ornata, a marine worm, contains two dehaloperoxidase genes."
Han K., Woodin S.A., Lincoln D.E., Fielman K.T., Ely B.
Mar. Biotechnol. 3:287-292(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
[3]"X-ray crystal structural analysis of the binding site in the ferric and oxyferrous forms of the recombinant heme dehaloperoxidase cloned from Amphitrite ornata."
de Serrano V., Chen Z., Davis M.F., Franzen S.
Acta Crystallogr. D 63:1094-1101(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.62 ANGSTROMS) OF 2-138 IN COMPLEX WITH HEME AND SULFATE.
[4]"Distal histidine conformational flexibility in dehaloperoxidase from Amphitrite ornata."
Chen Z., de Serrano V., Betts L., Franzen S.
Acta Crystallogr. D 65:34-40(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.26 ANGSTROMS) OF 2-138 IN COMPLEX WITH HEME.
[5]"The V59W mutation blocks the distal pocket of the hemoglobin dehaloperoxidase from Amphitrite ornata."
Davis M.F., de Serrano V., Gracz H., Vendreix F.A.P., Somasundram A., Negrerie M., Franzen S.
Submitted (OCT-2009) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (1.63 ANGSTROMS) OF 2-138 IN COMPLEX WITH HEME AND SULFATE.
[6]"X-ray structure of the metcyano form of dehaloperoxidase from Amphitrite ornata: evidence for photoreductive dissociation of the iron-cyanide bond."
de Serrano V.S., Davis M.F., Gaff J.F., Zhang Q., Chen Z., D'Antonio E.L., Bowden E.F., Rose R., Franzen S.
Acta Crystallogr. D 66:770-782(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.26 ANGSTROMS) OF 2-138 IN COMPLEX WITH CYANIDE; HEME AND SULFATE.
[7]"Internal binding of halogenated phenols in dehaloperoxidase-hemoglobin inhibits peroxidase function."
Thompson M.K., Davis M.F., de Serrano V., Nicoletti F.P., Howes B.D., Smulevich G., Franzen S.
Biophys. J. 99:1586-1595(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.06 ANGSTROMS) OF 2-138 IN COMPLEX WITH HEME AND SULFATE.
[8]"Characterization of the Inhibitor Binding Site of the Dehaloperoxidase-Hemoglobin from Amphitrite ornata using High-Pressure Xenon Derivatization."
de Serrano V.S., Franzen S.
Submitted (APR-2010) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (1.71 ANGSTROMS) OF 2-138 IN COMPLEX WITH HEME AND SULFATE.
[9]"Mutation of Methionine-86 in Dehaloperoxidase-hemoglobin: Effects of the Asp-His-Fe Triad in a 3/3 Globin."
D'Antonio E.L., D'Antonio J., Grazs H., de Serrano V.S., Thompson M.K., Ghiladi R.A., Franzen S., Bowden E.F.
Submitted (MAY-2010) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (2.19 ANGSTROMS) OF 2-138 IN COMPLEX WITH CYANIDE; HEME AND SULFATE.
[10]"The V59W mutation blocks the distal pocket of the hemoglobin - dehaloperoxidase from Amphitirite ornata."
Davis M.F., de Serrano V.S., Gracz H., Vendreix F.A.P., Somasundram A., Negrerie M., Franzen S.
Submitted (JUL-2010) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (1.72 ANGSTROMS) OF 2-138 IN COMPLEX WITH HEME AND SULFATE.
[11]"Effect of the H55D mutation on the kinetics and structure of dehaloperoxidase-hemoglobin A."
Zhao J., De Serrano V.S., S. Franzen.
Submitted (AUG-2010) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (1.72 ANGSTROMS) OF 2-138 IN COMPLEX WITH HEME AND SULFATE.
[12]"Effect of the H55D mutation on the kinetics and structure of dehaloperoxidase-hemoglobin A."
Zhao J., De Serrano V.S., Franzen S.
Submitted (AUG-2010) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (1.52 ANGSTROMS) OF 2-138 IN COMPLEX WITH SULFATE.
[13]"Structural evidence for stabilization of inhibitor binding by a protein cavity in the dehaloperoxidase-hemoglobin from Amphitrite ornata."
de Serrano V., Franzen S.
Biopolymers 98:27-35(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.22 ANGSTROMS) OF 2-138 IN COMPLEX WITH SULFATE.
[14]"A unique role for the distal histidine observed in carbonmonoxy dehaloperoxidase-hemoglobin A structures."
de Serrano V.S., Zhao J., Franzen S.
Submitted (FEB-2012) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (2.05 ANGSTROMS) OF 2-138 IN COMPLEX WITH HEME AND SULFATE.
[15]"The role of distal histidine in carbonmonoxide DHP structure."
Zhao J., Franzen S.
Submitted (SEP-2012) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (1.49 ANGSTROMS) OF 2-138 IN COMPLEX WITH HEME AND SULFATE.
[16]"Role of polarity of the distal pocket in the control of inhibitor binding in dehaloperoxidase-hemoglobin."
Plummer A., Thompson M.K., Franzen S.
Biochemistry 52:2218-2227(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.12 ANGSTROMS) OF 2-138 IN COMPLEX WITH HEME AND SULFATE.
[17]"Structural and kinetic study of an internal substrate binding site in dehaloperoxidase-hemoglobin A from Amphitrite ornata."
Zhao J., de Serrano V., Zhao J., Le P., Franzen S.
Biochemistry 52:2427-2439(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.44 ANGSTROMS) OF 2-138 IN COMPLEX WITH HEME AND SULFATE.
[18]"Complexes of dual-function hemoglobin/dehaloperoxidase with substrate 2,4,6-trichlorophenol are inhibitory and indicate binding of halophenol to compound I."
Wang C., Lovelace L.L., Sun S., Dawson J.H., Lebioda L.
Biochemistry 52:6203-6210(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.44 ANGSTROMS) OF 2-138 IN COMPLEX WITH HEME.
[19]"The role of T56 in controlling the flexibility of the distal histidine in dehaloperoxidase-hemoglobin from Amphitrite ornata."
Jiang S., Wright I., Swartz P., Franzen S.
Biochim. Biophys. Acta 1834:2020-2029(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.93 ANGSTROMS) IN COMPLEX WITH AZIDE; HEME AND SULFATE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF284381 mRNA. Translation: AAF97245.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1EW6X-ray1.78A/B2-138[»]
1EWAX-ray2.50A/B2-138[»]
2QFKX-ray1.62A/B2-138[»]
2QFNX-ray1.62A/B1-138[»]
3DR9X-ray1.26A/B2-138[»]
3K3UX-ray1.63A/B2-138[»]
3KUNX-ray1.26A/B2-138[»]
3KUOX-ray1.26A/B2-138[»]
3LB1X-ray1.76A/B2-138[»]
3LB2X-ray1.06A/B2-138[»]
3LB3X-ray1.85A/B2-138[»]
3LB4X-ray1.56A/B2-138[»]
3MOUX-ray1.71A/B2-138[»]
3MYMX-ray1.72A/B2-138[»]
3MYNX-ray2.19A/B2-138[»]
3O7NX-ray1.72A/B2-138[»]
3OJ1X-ray1.52A/B2-138[»]
3OK5X-ray1.72A/B2-138[»]
3ORDX-ray2.22A/B2-138[»]
4DWTX-ray2.05A/B2-138[»]
4DWUX-ray1.44A/B2-138[»]
4FH6X-ray1.44A/B2-138[»]
4FH7X-ray1.74A/B2-138[»]
4GZGX-ray1.49A/B2-138[»]
4HSWX-ray1.22A/B2-138[»]
4HSXX-ray1.12A/B2-138[»]
4ILZX-ray1.91A/B2-138[»]
4JACX-ray1.93A/B1-138[»]
4KMVX-ray1.44A/B2-138[»]
4KMWX-ray1.79A/B2-138[»]
4KN3X-ray1.78A/B2-138[»]
ProteinModelPortalQ9NAV8.
SMRQ9NAV8. Positions 2-138.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.10.490.10. 1 hit.
InterProIPR000971. Globin.
IPR009050. Globin-like.
IPR012292. Globin_dom.
[Graphical view]
PfamPF00042. Globin. 1 hit.
[Graphical view]
SUPFAMSSF46458. SSF46458. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceQ9NAV8.

Entry information

Entry nameQ9NAV8_9ANNE
AccessionPrimary (citable) accession number: Q9NAV8
Entry history
Integrated into UniProtKB/TrEMBL: October 1, 2000
Last sequence update: October 1, 2000
Last modified: April 16, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)