Q9NAV8 (Q9NAV8_9ANNE) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names	Submitted name: Dehaloperoxidase A EMBL AAF97245.1
Organism	Amphitrite ornata EMBL AAF97245.1
Taxonomic identifier	129555 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Lophotrochozoa › Annelida › Polychaeta › Scolecida › Terebellida › Amphitrite

Protein attributes

Sequence length	138 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Sequence similarities

Belongs to the globin family. RuleBase RU000356

Ontologies

Keywords
Biological process	Oxygen transport RuleBase RU000356 Transport
Ligand	Heme RuleBase RU000356 PDB 1EWA PDB 2QFK PDB 2QFN PDB 3DR9 PDB 3K3U PDB 3KUN PDB 3LB1 PDB 3LB2 PDB 3LB3 PDB 3LB4 PDB 1EW6 PDB 3MOU PDB 3MYN PDB 3O7N Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase EMBL AAF97245.1
Technical term	3D-structure PDB 4DWU PDB 1EWA PDB 2QFK PDB 2QFN PDB 3DR9 PDB 4DWT PDB 3K3U PDB 3KUN PDB 3LB1 PDB 3LB2 PDB 3LB3 PDB 3LB4 PDB 1EW6 PDB 3MOU PDB 3MYN PDB 3MYM PDB 3KUO PDB 3O7N PDB 3OJ1 PDB 3OK5 PDB 3ORD PDB 4FH6 PDB 4FH7 PDB 4ILZ
Gene Ontology (GO)
Molecular_function	heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro oxygen binding Inferred from electronic annotation. Source: InterPro oxygen transporter activity Inferred from electronic annotation. Source: UniProtKB-KW peroxidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Metal binding	90	1	Iron (heme axial ligand); via tele nitrogen PDB 1EWA PDB 2QFK PDB 2QFN PDB 3DR9 PDB 3K3U PDB 3KUN PDB 3LB1 PDB 3LB2 PDB 3LB3 PDB 3LB4 PDB 1EW6 PDB 3MOU PDB 3MYN PDB 3O7N

Sequences

	Sequence		Length	Mass (Da)
	Q9NAV8 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: E63F8C82A7CB90A9	FASTA	138	15,660
	10 20 30 40 50 60 MGFKQDIATI RGDLRTYAQD IFLAFLNKYP DERRYFKNYV GKSDQELKSM AKFGDHTEKV 70 80 90 100 110 120 FNLMMEVADR ATDCVPLASD ANTLVQMKQH SSLTTGNFEK LFVALVEYMR ASGQSFDSQS 130 WDRFGKNLVS ALSSAGMK

References

[1]	"The crystal structure and amino acid sequence of dehaloperoxidase from Amphitrite ornata indicate common ancestry with globins." LaCount M.W., Zhang E., Chen Y.P., Han K., Whitton M.M., Lincoln D.E., Woodin S.A., Lebioda L. J. Biol. Chem. 275:18712-18716(2000) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.78 ANGSTROMS) OF 2-138 IN COMPLEX WITH HEME.
[2]	"Amphitrite ornata, a marine worm, contains two dehaloperoxidase genes." Han K., Woodin S.A., Lincoln D.E., Fielman K.T., Ely B. Mar. Biotechnol. 3:287-292(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[3]	"X-ray crystal structural analysis of the binding site in the ferric and oxyferrous forms of the recombinant heme dehaloperoxidase cloned from Amphitrite ornata." de Serrano V., Chen Z., Davis M.F., Franzen S. Acta Crystallogr. D 63:1094-1101(2007) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.62 ANGSTROMS) OF 2-138 IN COMPLEX WITH HEME.
[4]	"Distal histidine conformational flexibility in dehaloperoxidase from Amphitrite ornata." Chen Z., de Serrano V., Betts L., Franzen S. Acta Crystallogr. D 65:34-40(2009) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.26 ANGSTROMS) OF 2-138 IN COMPLEX WITH HEME.
[5]	"The V59W mutation blocks the distal pocket of the hemoglobin dehaloperoxidase from Amphitrite ornata." Davis M.F., de Serrano V., Gracz H., Vendreix F.A.P., Somasundram A., Negrerie M., Franzen S. Submitted (OCT-2009) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (1.63 ANGSTROMS) OF 2-138 IN COMPLEX WITH HEME.
[6]	"X-ray structure of the metcyano form of dehaloperoxidase from Amphitrite ornata: evidence for photoreductive dissociation of the iron-cyanide bond." de Serrano V.S., Davis M.F., Gaff J.F., Zhang Q., Chen Z., D'Antonio E.L., Bowden E.F., Rose R., Franzen S. Acta Crystallogr. D 66:770-782(2010) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.26 ANGSTROMS) OF 2-138 IN COMPLEX WITH HEME.
[7]	"Internal binding of halogenated phenols in dehaloperoxidase-hemoglobin inhibits peroxidase function." Thompson M.K., Davis M.F., de Serrano V., Nicoletti F.P., Howes B.D., Smulevich G., Franzen S. Biophys. J. 99:1586-1595(2010) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.06 ANGSTROMS) OF 2-138 IN COMPLEX WITH HEME.
[8]	"Characterization of the Inhibitor Binding Site of the Dehaloperoxidase-Hemoglobin from Amphitrite ornata using High-Pressure Xenon Derivatization." de Serrano V.S., Franzen S. Submitted (APR-2010) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (1.71 ANGSTROMS) OF 2-138 IN COMPLEX WITH HEME.
[9]	"Mutation of Methionine-86 in Dehaloperoxidase-hemoglobin: Effects of the Asp-His-Fe Triad in a 3/3 Globin." D'Antonio E.L., D'Antonio J., Grazs H., de Serrano V.S., Thompson M.K., Ghiladi R.A., Franzen S., Bowden E.F. Submitted (MAY-2010) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.19 ANGSTROMS) OF 2-138 IN COMPLEX WITH HEME.
[10]	"The V59W mutation blocks the distal pocket of the hemoglobin - dehaloperoxidase from Amphitirite ornata." Davis M.F., de Serrano V.S., Gracz H., Vendreix F.A.P., Somasundram A., Negrerie M., Franzen S. Submitted (JUL-2010) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (1.72 ANGSTROMS) OF 2-138 IN COMPLEX WITH HEME.
[11]	"Effect of the H55D mutation on the kinetics and structure of dehaloperoxidase-hemoglobin A." Zhao J., De Serrano V.S., Franzen S. Submitted (AUG-2010) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (1.52 ANGSTROMS) OF 2-138.
[12]	"Effect of the H55D mutation on the kinetics and structure of dehaloperoxidase-hemoglobin A." Zhao J., De Serrano V.S., S. Franzen. Submitted (AUG-2010) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (1.72 ANGSTROMS) OF 2-138.
[13]	"Structural evidence for stabilization of inhibitor binding by a protein cavity in the dehaloperoxidase-hemoglobin from Amphitrite ornata." de Serrano V., Franzen S. Biopolymers 98:27-35(2012) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.22 ANGSTROMS) OF 2-138.
[14]	"A unique role for the distal histidine observed in carbonmonoxy dehaloperoxidase-hemoglobin A structures." de Serrano V.S., Zhao J., Franzen S. Submitted (FEB-2012) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.05 ANGSTROMS) OF 2-138.
[15]	"Structural and Kinetic Study of an Internal Substrate Binding Site in Dehaloperoxidase-Hemoglobin A from Amphitrite ornata." Zhao J., de Serrano V., Zhao J., Le P., Franzen S. Biochemistry 52:2427-2439(2013) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.44 ANGSTROMS) OF 2-138.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF284381 mRNA. Translation: AAF97245.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1EW6	X-ray	1.78	A/B	2-138	[»]
1EWA	X-ray	2.50	A/B	2-138	[»]
2QFK	X-ray	1.62	A/B	2-138	[»]
2QFN	X-ray	1.62	A/B	1-138	[»]
3DR9	X-ray	1.26	A/B	2-138	[»]
3K3U	X-ray	1.63	A/B	2-138	[»]
3KUN	X-ray	1.26	A/B	2-138	[»]
3KUO	X-ray	1.26	A/B	2-138	[»]
3LB1	X-ray	1.76	A/B	2-138	[»]
3LB2	X-ray	1.06	A/B	2-138	[»]
3LB3	X-ray	1.85	A/B	2-138	[»]
3LB4	X-ray	1.56	A/B	2-138	[»]
3MOU	X-ray	1.71	A/B	2-138	[»]
3MYM	X-ray	1.72	A/B	2-138	[»]
3MYN	X-ray	2.19	A/B	2-138	[»]
3O7N	X-ray	1.72	A/B	2-138	[»]
3OJ1	X-ray	1.52	A/B	2-138	[»]
3OK5	X-ray	1.72	A/B	2-138	[»]
3ORD	X-ray	2.22	A/B	2-138	[»]
4DWT	X-ray	2.05	A/B	2-138	[»]
4DWU	X-ray	1.44	A/B	2-138	[»]
4FH6	X-ray	1.44	A/B	2-138	[»]
4FH7	X-ray	1.74	A/B	2-138	[»]
4ILZ	X-ray	1.91	A/B	2-138	[»]

ProteinModelPortal

Q9NAV8.

SMR

Q9NAV8. Positions 2-138.

ModBase

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Protocols and materials databases

StructuralBiologyKnowledgebase

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Family and domain databases

Gene3D

1.10.490.10. 1 hit.

InterPro

IPR000971. Globin.
IPR009050. Globin-like.
IPR012292. Globin_dom.
[Graphical view]

Pfam

PF00042. Globin. 1 hit.
[Graphical view]

SUPFAM

SSF46458. Globin_like. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

Q9NAV8.

Entry information

Entry name

Q9NAV8_9ANNE

Accession

Primary (citable) accession number: Q9NAV8

Entry history

Integrated into UniProtKB/TrEMBL:	October 1, 2000
Last sequence update:	October 1, 2000
Last modified:	May 29, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)