Q9N4X8 (GSTPA_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutathione S-transferase P 10
EC=2.5.1.18

Alternative name(s):
GST 5.4
GST class-pi
GSTP2-2

Gene names

Name:	gst-10
ORF Names:	Y45G12C.2

Organism

Caenorhabditis elegans [Reference proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	210 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Responsible for approximately one-third of 4-hydroxy-2-nonenal conjugation. Ref.1 Ref.3 Ref.4
Catalytic activity	RX + glutathione = HX + R-S-glutathione. UniProtKB P10299
Subunit structure	Homodimer Potential. UniProtKB P10299
Tissue specificity	Expressed in cells at the mouth and adjacent to the pharyngeal bulbs of the head and also in the tail. Ref.3
Disruption phenotype	Increase in susceptibility to 4-hydroxy-2-nonenal, paraquat and heat shock and a reduced lifespan. Ref.4
Sequence similarities	Belongs to the GST superfamily. Pi family. UniProtKB P10299 Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.
Biophysicochemical properties	Kinetic parameters: KM=50 µM for 4-HNE Ref.3 Vmax=10.3 µmol/min/mg enzyme Ref.3

Ontologies

Keywords
Molecular function	Transferase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	determination of adult lifespan Inferred from mutant phenotype Ref.4. Source: UniProtKB glutathione metabolic process Traceable author statement Ref.3. Source: UniProtKB oxidation-reduction process Inferred from mutant phenotype Ref.4. Source: WormBase response to UV-C Inferred from mutant phenotype Ref.3. Source: UniProtKB response to hydrogen peroxide Inferred from mutant phenotype Ref.3. Source: UniProtKB
Molecular_function	glutathione transferase activity Traceable author statement Ref.3. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Chain	2 – 210	209	Glutathione S-transferase P 10 Ref.1		PRO_0000185912
Regions
	Domain	2 – 81	80	GST N-terminal
	Domain	83 – 200	118	GST C-terminal
	Region	52 – 53	2	Glutathione binding By similarity
	Region	65 – 66	2	Glutathione binding By similarity
Sites
	Binding site	8	1	Glutathione By similarity
	Binding site	41	1	Glutathione By similarity
	Binding site	45	1	Glutathione By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9N4X8 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 31AF3BC1ED43594B	FASTA	210	24,796
	10 20 30 40 50 60 MAVPQLYYFT IRGFGEYIRL LFLDNGIKFE DIRFDYEGNE WQEFKKGMLL GQLPCLKVDG 70 80 90 100 110 120 QEIVQTGAIM RHLGRVHGLN GSNEQEATFL DMFFEGVRDV RMKYVRYIYY DEGTREDCVN 130 140 150 160 170 180 KTIPEALVKL EELFKAHSGD FIIGNKISYA DYILFEELDV YHVLDANILD KFPTLKSFWE 190 200 210 RMWKRPNLNA YLEKRKADKV WINAIEKGMN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Invertebrate glutathione transferases conjugating 4-hydroxynonenal: CeGST 5.4 from Caenorhabditis elegans." Engle M.R., Singh S.P., Nanduri B., Ji X., Zimniak P. Chem. Biol. Interact. 133:244-248(2001) Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 2-11, FUNCTION.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[3]	"Lifespan and stress resistance of Caenorhabditis elegans are increased by expression of glutathione transferases capable of metabolizing the lipid peroxidation product 4-hydroxynonenal." Ayyadevara S., Engle M.R., Singh S.P., Dandapat A., Lichti C.F., Benes H., Shmookler Reis R.J., Liebau E., Zimniak P. Aging Cell 4:257-271(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
[4]	"Lifespan extension in hypomorphic daf-2 mutants of Caenorhabditis elegans is partially mediated by glutathione transferase CeGSTP2-2." Ayyadevara S., Dandapat A., Singh S.P., Benes H., Zimniak L., Reis R.J.S., Zimniak P. Aging Cell 4:299-307(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FO080245 Genomic DNA. Translation: CCD62297.1.
RefSeq	NP_503701.1. NM_071300.7.
3D structure databases
ProteinModelPortal	Q9N4X8.
SMR	Q9N4X8. Positions 5-204.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9N4X8. 1 interaction.
STRING	6239.Y45G12C.2.1.
Proteomic databases
PaxDb	Q9N4X8.
PRIDE	Q9N4X8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	Y45G12C.2; Y45G12C.2; Y45G12C.2.
GeneID	178725.
KEGG	cel:CELE_Y45G12C.2.
UCSC	Y45G12C.2.1. c. elegans.
Organism-specific databases
CTD	178725.
WormBase	Y45G12C.2; CE21937; WBGene00001758; gst-10.
Phylogenomic databases
eggNOG	NOG266414.
GeneTree	ENSGT00550000074559.
HOGENOM	HOG000115733.
InParanoid	Q9N4X8.
KO	K00799.
OMA	ADKVWIN.
Family and domain databases
Gene3D	1.20.1050.10. 1 hit. 3.40.30.10. 1 hit.
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR004046. GST_C. IPR012336. Thioredoxin-like_fold. [Graphical view]
Pfam	PF00043. GST_C. 1 hit. PF02798. GST_N. 1 hit. [Graphical view]
SUPFAM	SSF47616. GST_C_like. 1 hit. SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	902290.

Entry information

Entry name

GSTPA_CAEEL

Accession

Primary (citable) accession number: Q9N4X8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 28, 2003
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 84 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project