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Q9N4X8

- GSTPA_CAEEL

UniProt

Q9N4X8 - GSTPA_CAEEL

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Protein

Glutathione S-transferase P 10

Gene

gst-10

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Responsible for approximately one-third of 4-hydroxy-2-nonenal conjugation.3 Publications

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.By similarity

Kineticsi

  1. KM=50 µM for 4-HNE1 Publication

Vmax=10.3 µmol/min/mg enzyme1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei8 – 81GlutathioneBy similarity
Binding sitei41 – 411GlutathioneBy similarity
Binding sitei45 – 451GlutathioneBy similarity

GO - Molecular functioni

  1. glutathione transferase activity Source: UniProtKB

GO - Biological processi

  1. determination of adult lifespan Source: UniProtKB
  2. glutathione metabolic process Source: UniProtKB
  3. heat acclimation Source: UniProtKB
  4. oxidation-reduction process Source: WormBase
  5. response to hydrogen peroxide Source: UniProtKB
  6. response to UV-C Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Enzyme and pathway databases

ReactomeiREACT_184548. Detoxification of Reactive Oxygen Species.
REACT_263468. Glutathione conjugation.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase P 10 (EC:2.5.1.18)
Alternative name(s):
GST 5.4
GST class-pi
GSTP2-2
Gene namesi
Name:gst-10Imported
ORF Names:Y45G12C.2
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome V

Organism-specific databases

WormBaseiY45G12C.2; CE21937; WBGene00001758; gst-10.

Pathology & Biotechi

Disruption phenotypei

Increase in susceptibility to 4-hydroxy-2-nonenal, paraquat and heat shock and a reduced lifespan.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 210209Glutathione S-transferase P 10PRO_0000185912Add
BLAST

Proteomic databases

PaxDbiQ9N4X8.
PRIDEiQ9N4X8.

Expressioni

Tissue specificityi

Expressed in cells at the mouth and adjacent to the pharyngeal bulbs of the head and also in the tail.1 Publication

Interactioni

Subunit structurei

Homodimer.By similarityCurated

Protein-protein interaction databases

IntActiQ9N4X8. 1 interaction.
STRINGi6239.Y45G12C.2.1.

Structurei

3D structure databases

ProteinModelPortaliQ9N4X8.
SMRiQ9N4X8. Positions 5-204.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 8180GST N-terminalAdd
BLAST
Domaini83 – 200118GST C-terminalAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni52 – 532Glutathione bindingBy similarity
Regioni65 – 662Glutathione bindingBy similarity

Sequence similaritiesi

Belongs to the GST superfamily. Pi family.By similarity
Contains 1 GST C-terminal domain.Curated
Contains 1 GST N-terminal domain.Curated

Phylogenomic databases

eggNOGiNOG266414.
GeneTreeiENSGT00670000097856.
HOGENOMiHOG000115733.
InParanoidiQ9N4X8.
KOiK00799.
OMAiADKVWIN.
OrthoDBiEOG79CZ0K.
PhylomeDBiQ9N4X8.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9N4X8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAVPQLYYFT IRGFGEYIRL LFLDNGIKFE DIRFDYEGNE WQEFKKGMLL
60 70 80 90 100
GQLPCLKVDG QEIVQTGAIM RHLGRVHGLN GSNEQEATFL DMFFEGVRDV
110 120 130 140 150
RMKYVRYIYY DEGTREDCVN KTIPEALVKL EELFKAHSGD FIIGNKISYA
160 170 180 190 200
DYILFEELDV YHVLDANILD KFPTLKSFWE RMWKRPNLNA YLEKRKADKV
210
WINAIEKGMN
Length:210
Mass (Da):24,796
Last modified:January 23, 2007 - v3
Checksum:i31AF3BC1ED43594B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080245 Genomic DNA. Translation: CCD62297.1.
RefSeqiNP_503701.1. NM_071300.7.
UniGeneiCel.34291.

Genome annotation databases

EnsemblMetazoaiY45G12C.2; Y45G12C.2; WBGene00001758.
GeneIDi178725.
KEGGicel:CELE_Y45G12C.2.
UCSCiY45G12C.2.1. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080245 Genomic DNA. Translation: CCD62297.1 .
RefSeqi NP_503701.1. NM_071300.7.
UniGenei Cel.34291.

3D structure databases

ProteinModelPortali Q9N4X8.
SMRi Q9N4X8. Positions 5-204.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q9N4X8. 1 interaction.
STRINGi 6239.Y45G12C.2.1.

Proteomic databases

PaxDbi Q9N4X8.
PRIDEi Q9N4X8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai Y45G12C.2 ; Y45G12C.2 ; WBGene00001758 .
GeneIDi 178725.
KEGGi cel:CELE_Y45G12C.2.
UCSCi Y45G12C.2.1. c. elegans.

Organism-specific databases

CTDi 178725.
WormBasei Y45G12C.2 ; CE21937 ; WBGene00001758 ; gst-10.

Phylogenomic databases

eggNOGi NOG266414.
GeneTreei ENSGT00670000097856.
HOGENOMi HOG000115733.
InParanoidi Q9N4X8.
KOi K00799.
OMAi ADKVWIN.
OrthoDBi EOG79CZ0K.
PhylomeDBi Q9N4X8.

Enzyme and pathway databases

Reactomei REACT_184548. Detoxification of Reactive Oxygen Species.
REACT_263468. Glutathione conjugation.

Miscellaneous databases

NextBioi 902290.

Family and domain databases

Gene3Di 1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProi IPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view ]
Pfami PF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view ]
SUPFAMi SSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEi PS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Invertebrate glutathione transferases conjugating 4-hydroxynonenal: CeGST 5.4 from Caenorhabditis elegans."
    Engle M.R., Singh S.P., Nanduri B., Ji X., Zimniak P.
    Chem. Biol. Interact. 133:244-248(2001)
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 2-11, FUNCTION.
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  3. "Lifespan and stress resistance of Caenorhabditis elegans are increased by expression of glutathione transferases capable of metabolizing the lipid peroxidation product 4-hydroxynonenal."
    Ayyadevara S., Engle M.R., Singh S.P., Dandapat A., Lichti C.F., Benes H., Shmookler Reis R.J., Liebau E., Zimniak P.
    Aging Cell 4:257-271(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
  4. "Lifespan extension in hypomorphic daf-2 mutants of Caenorhabditis elegans is partially mediated by glutathione transferase CeGSTP2-2."
    Ayyadevara S., Dandapat A., Singh S.P., Benes H., Zimniak L., Reis R.J.S., Zimniak P.
    Aging Cell 4:299-307(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiGSTPA_CAEEL
AccessioniPrimary (citable) accession number: Q9N4X8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 28, 2003
Last sequence update: January 23, 2007
Last modified: November 26, 2014
This is version 92 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3