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Q9N4X8

- GSTPA_CAEEL

UniProt

Q9N4X8 - GSTPA_CAEEL

Protein

Glutathione S-transferase P 10

Gene

gst-10

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 91 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Responsible for approximately one-third of 4-hydroxy-2-nonenal conjugation.3 Publications

    Catalytic activityi

    RX + glutathione = HX + R-S-glutathione.By similarity

    Kineticsi

    1. KM=50 µM for 4-HNE1 Publication

    Vmax=10.3 µmol/min/mg enzyme1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei8 – 81GlutathioneBy similarity
    Binding sitei41 – 411GlutathioneBy similarity
    Binding sitei45 – 451GlutathioneBy similarity

    GO - Molecular functioni

    1. glutathione transferase activity Source: UniProtKB

    GO - Biological processi

    1. determination of adult lifespan Source: UniProtKB
    2. glutathione metabolic process Source: UniProtKB
    3. heat acclimation Source: UniProtKB
    4. oxidation-reduction process Source: WormBase
    5. response to hydrogen peroxide Source: UniProtKB
    6. response to UV-C Source: UniProtKB

    Keywords - Molecular functioni

    Transferase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione S-transferase P 10 (EC:2.5.1.18)
    Alternative name(s):
    GST 5.4
    GST class-pi
    GSTP2-2
    Gene namesi
    Name:gst-10Imported
    ORF Names:Y45G12C.2
    OrganismiCaenorhabditis elegans
    Taxonomic identifieri6239 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
    ProteomesiUP000001940: Chromosome V

    Organism-specific databases

    WormBaseiY45G12C.2; CE21937; WBGene00001758; gst-10.

    Pathology & Biotechi

    Disruption phenotypei

    Increase in susceptibility to 4-hydroxy-2-nonenal, paraquat and heat shock and a reduced lifespan.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 210209Glutathione S-transferase P 10PRO_0000185912Add
    BLAST

    Proteomic databases

    PaxDbiQ9N4X8.
    PRIDEiQ9N4X8.

    Expressioni

    Tissue specificityi

    Expressed in cells at the mouth and adjacent to the pharyngeal bulbs of the head and also in the tail.1 Publication

    Interactioni

    Subunit structurei

    Homodimer.By similarityCurated

    Protein-protein interaction databases

    IntActiQ9N4X8. 1 interaction.
    STRINGi6239.Y45G12C.2.1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9N4X8.
    SMRiQ9N4X8. Positions 5-204.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 8180GST N-terminalAdd
    BLAST
    Domaini83 – 200118GST C-terminalAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni52 – 532Glutathione bindingBy similarity
    Regioni65 – 662Glutathione bindingBy similarity

    Sequence similaritiesi

    Belongs to the GST superfamily. Pi family.By similarity
    Contains 1 GST C-terminal domain.Curated
    Contains 1 GST N-terminal domain.Curated

    Phylogenomic databases

    eggNOGiNOG266414.
    GeneTreeiENSGT00670000097856.
    HOGENOMiHOG000115733.
    InParanoidiQ9N4X8.
    KOiK00799.
    OMAiADKVWIN.
    OrthoDBiEOG79CZ0K.
    PhylomeDBiQ9N4X8.

    Family and domain databases

    Gene3Di1.20.1050.10. 1 hit.
    3.40.30.10. 1 hit.
    InterProiIPR010987. Glutathione-S-Trfase_C-like.
    IPR004045. Glutathione_S-Trfase_N.
    IPR004046. GST_C.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view]
    PfamiPF00043. GST_C. 1 hit.
    PF02798. GST_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF47616. SSF47616. 1 hit.
    SSF52833. SSF52833. 1 hit.
    PROSITEiPS50405. GST_CTER. 1 hit.
    PS50404. GST_NTER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9N4X8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAVPQLYYFT IRGFGEYIRL LFLDNGIKFE DIRFDYEGNE WQEFKKGMLL    50
    GQLPCLKVDG QEIVQTGAIM RHLGRVHGLN GSNEQEATFL DMFFEGVRDV 100
    RMKYVRYIYY DEGTREDCVN KTIPEALVKL EELFKAHSGD FIIGNKISYA 150
    DYILFEELDV YHVLDANILD KFPTLKSFWE RMWKRPNLNA YLEKRKADKV 200
    WINAIEKGMN 210
    Length:210
    Mass (Da):24,796
    Last modified:January 23, 2007 - v3
    Checksum:i31AF3BC1ED43594B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FO080245 Genomic DNA. Translation: CCD62297.1.
    RefSeqiNP_503701.1. NM_071300.7.
    UniGeneiCel.34291.

    Genome annotation databases

    EnsemblMetazoaiY45G12C.2; Y45G12C.2; WBGene00001758.
    GeneIDi178725.
    KEGGicel:CELE_Y45G12C.2.
    UCSCiY45G12C.2.1. c. elegans.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FO080245 Genomic DNA. Translation: CCD62297.1 .
    RefSeqi NP_503701.1. NM_071300.7.
    UniGenei Cel.34291.

    3D structure databases

    ProteinModelPortali Q9N4X8.
    SMRi Q9N4X8. Positions 5-204.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q9N4X8. 1 interaction.
    STRINGi 6239.Y45G12C.2.1.

    Proteomic databases

    PaxDbi Q9N4X8.
    PRIDEi Q9N4X8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai Y45G12C.2 ; Y45G12C.2 ; WBGene00001758 .
    GeneIDi 178725.
    KEGGi cel:CELE_Y45G12C.2.
    UCSCi Y45G12C.2.1. c. elegans.

    Organism-specific databases

    CTDi 178725.
    WormBasei Y45G12C.2 ; CE21937 ; WBGene00001758 ; gst-10.

    Phylogenomic databases

    eggNOGi NOG266414.
    GeneTreei ENSGT00670000097856.
    HOGENOMi HOG000115733.
    InParanoidi Q9N4X8.
    KOi K00799.
    OMAi ADKVWIN.
    OrthoDBi EOG79CZ0K.
    PhylomeDBi Q9N4X8.

    Miscellaneous databases

    NextBioi 902290.

    Family and domain databases

    Gene3Di 1.20.1050.10. 1 hit.
    3.40.30.10. 1 hit.
    InterProi IPR010987. Glutathione-S-Trfase_C-like.
    IPR004045. Glutathione_S-Trfase_N.
    IPR004046. GST_C.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view ]
    Pfami PF00043. GST_C. 1 hit.
    PF02798. GST_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47616. SSF47616. 1 hit.
    SSF52833. SSF52833. 1 hit.
    PROSITEi PS50405. GST_CTER. 1 hit.
    PS50404. GST_NTER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Invertebrate glutathione transferases conjugating 4-hydroxynonenal: CeGST 5.4 from Caenorhabditis elegans."
      Engle M.R., Singh S.P., Nanduri B., Ji X., Zimniak P.
      Chem. Biol. Interact. 133:244-248(2001)
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 2-11, FUNCTION.
    2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
      The C. elegans sequencing consortium
      Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Bristol N2.
    3. "Lifespan and stress resistance of Caenorhabditis elegans are increased by expression of glutathione transferases capable of metabolizing the lipid peroxidation product 4-hydroxynonenal."
      Ayyadevara S., Engle M.R., Singh S.P., Dandapat A., Lichti C.F., Benes H., Shmookler Reis R.J., Liebau E., Zimniak P.
      Aging Cell 4:257-271(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
    4. "Lifespan extension in hypomorphic daf-2 mutants of Caenorhabditis elegans is partially mediated by glutathione transferase CeGSTP2-2."
      Ayyadevara S., Dandapat A., Singh S.P., Benes H., Zimniak L., Reis R.J.S., Zimniak P.
      Aging Cell 4:299-307(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiGSTPA_CAEEL
    AccessioniPrimary (citable) accession number: Q9N4X8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 28, 2003
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 91 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programCaenorhabditis annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Caenorhabditis elegans
      Caenorhabditis elegans: entries, gene names and cross-references to WormBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3