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Q9N4G4 (MED1_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mediator of RNA polymerase II transcription subunit 1.1
Alternative name(s):
Mediator complex subunit 1.1
Suppressor of pal-1 protein 3
Gene names
Name:sop-3
Synonyms:mdt-1.1
ORF Names:Y71F9B.10
OrganismCaenorhabditis elegans [Reference proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis

Protein attributes

Sequence length1475 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors By similarity.

Subunit structure

Component of the Mediator complex By similarity.

Subcellular location

Nucleus By similarity.

Sequence similarities

Belongs to the Mediator complex subunit 1 family.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
   DomainCoiled coil
   Molecular functionActivator
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentmediator complex

Inferred from electronic annotation. Source: InterPro

   Molecular_functionRNA polymerase II transcription cofactor activity

Inferred from electronic annotation. Source: InterPro

protein binding

Inferred from physical interaction PubMed 19447967PubMed 23549480. Source: IntAct

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

T04C9.1Q8MPT26EBI-2315681,EBI-2315635

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform a (identifier: Q9N4G4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform b (identifier: Q9N4G4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     536-544: VTVLEVGPA → ELYALTTSK
     545-1475: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 14751475Mediator of RNA polymerase II transcription subunit 1.1
PRO_0000302025

Regions

Coiled coil1098 – 113538 Potential
Compositional bias755 – 79945Gln-rich
Compositional bias820 – 88263Pro-rich
Compositional bias1280 – 1441162Pro-rich

Natural variations

Alternative sequence536 – 5449VTVLEVGPA → ELYALTTSK in isoform b.
VSP_027908
Alternative sequence545 – 1475931Missing in isoform b.
VSP_027909

Sequences

Sequence LengthMass (Da)Tools
Isoform a [UniParc].

Last modified October 1, 2001. Version 2.
Checksum: C0A80E70DE9CC98B

FASTA1,475164,547
        10         20         30         40         50         60 
MIIEERKNHE LNLESIDRDL RMENVRQSAE KLGWEHFARS VRRNLLEKRQ TLDARTRLDV 

        70         80         90        100        110        120 
LGSLVFMKEH LPIDNDATIA RKVQQLSEGL GEHCVFSTQN SGFSIRNPDV TIDIGVAEDQ 

       130        140        150        160        170        180 
ISTCKIGYFG QPLTDAPGAL ALMKNGEFSK LRDSITAILS SLPKEITLSE KNACKEAFRA 

       190        200        210        220        230        240 
LDQLLIRDAA DSSFTAINTG KYGFYAPRNE LRSGRIYYVA EPVLIRLAEK EGKTKADASY 

       250        260        270        280        290        300 
LDLLPYIEIS FFKHDKPSKL PVFTQTGEWS TFNDANVCIC VRFNQGFLLS QQTIRKLSHI 

       310        320        330        340        350        360 
VPKSPLVRNY VNFYRYMTGR VGVKSNLKLL TQFPDEGDIQ QQYQVDSKML TKEDDVVAME 

       370        380        390        400        410        420 
MYLSDFRDLS KLIETLRSEW KHASLWESIL AICESSKGEQ KEVNAVDMQL VLRRTEFFLQ 

       430        440        450        460        470        480 
FDTDYGSMTM QIFEKSPSNY VVKTTSQITG ESPSLDFDKM LTEKLNSTWS IPATLTFAIT 

       490        500        510        520        530        540 
DLKCRINSVL NCVISFDSSS SSDKQHQNYW SISNNNTQLM QSAAQKSAAK CTKKKVTVLE 

       550        560        570        580        590        600 
VGPAIEPTQL VYTRENDEEF QGSLLSSFGE VDDYYVKTST IGRTRPQPRP KREPNQLTSM 

       610        620        630        640        650        660 
DMMSAELDKS RHTMEGSMRD AQTSSFLDAA RVRMKQSIGA AHAVGLASHQ PPASPVAAAA 

       670        680        690        700        710        720 
AAPGGPMRHN SYPSGFEPPP SVAPYDFPAD PSKKQRKQRA KKQPGEEPPA GRGKGGKKGR 

       730        740        750        760        770        780 
GAGAVGAAAA GGRKSAGGAG ENPFGMDPMR PPMLQRSSSE QHNPNPHQMS QYQMQQYQQN 

       790        800        810        820        830        840 
QQFRMHQMQQ QQQQQFQMQS PKNPLVPAYT DEDSDEECDP PPPPKPQVST SSRMSSVPSH 

       850        860        870        880        890        900 
PPPPPQQLGN PMVGYPGMPL QSPNHLPLTP SPLSAPPKPF SPEQQHHFGM KMRETSYWKE 

       910        920        930        940        950        960 
LERLETKPDV EKLKQQLSSS SSSSQAEASD PPSTSTEAQD PPKPSSSSAP PPPKKKLGLE 

       970        980        990       1000       1010       1020 
AAISKIRGQQ EQALQKQLQQ QESVESELAT PHPGSSGAPP LAPHQVNRAR NLNDIFDDEA 

      1030       1040       1050       1060       1070       1080 
DDSSPTADIK PSLQALQKPT DTSSQSTSSE PPPKKEPADE QPEREKEKLI LKIPKMLKPV 

      1090       1100       1110       1120       1130       1140 
VDDRREDRKE RDRDRDRDRD RDEDREKVRD KEDKAQREKD KKEKERERRR QRDRDRTEQK 

      1150       1160       1170       1180       1190       1200 
KSDREKEPSK KRKLEKKDEK EKDRREPERK KGKSDGKEYS KASTTSLIPT LSLKNFRIPK 

      1210       1220       1230       1240       1250       1260 
KDTVEEEKKD VKEEAPGPSS STESKKEPPS AAPITRKEST TAPVAPAVQQ QQQRKESFTA 

      1270       1280       1290       1300       1310       1320 
LPSLPEQQQH HREPLLKKKP PLQPPPPPQM TGPPIGIYPG PPAPGNLPGS SRPSGNRKQP 

      1330       1340       1350       1360       1370       1380 
LPPPPPMIRG PPPDQMYRDR SGGGGGSMRG GFPPASHYHG GTSGVNKQVA SYAQGLPPGM 

      1390       1400       1410       1420       1430       1440 
GPPVVKPHGN NYQASQWVRP PTHRDSSHSY HGMPSLGPPQ LQREPPAPPP PQMIPLPKDP 

      1450       1460       1470 
PVLREHPREH RAHRGGADDD GPDSPEEGTL RIDDE 

« Hide

Isoform b [UniParc].

Checksum: 478ADA6030DDA18D
Show »

FASTA54461,840

References

« Hide 'large scale' references
[1]"The novel C. elegans gene sop-3 modulates Wnt signaling to regulate Hox gene expression."
Zhang H., Emmons S.W.
Development 128:767-777(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A).
[2]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
Strain: Bristol N2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF308860 mRNA. Translation: AAG45416.1.
FO081276 Genomic DNA. Translation: CCD70417.1.
FO081276 Genomic DNA. Translation: CCD70418.1.
RefSeqNP_001021811.1. NM_001026640.3. [Q9N4G4-2]
NP_740821.1. NM_170837.3. [Q9N4G4-1]
UniGeneCel.17584.

3D structure databases

ProteinModelPortalQ9N4G4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid37321. 3 interactions.
IntActQ9N4G4. 1 interaction.

Proteomic databases

PaxDbQ9N4G4.
PRIDEQ9N4G4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaY71F9B.10a; Y71F9B.10a; WBGene00004946. [Q9N4G4-1]
GeneID171840.
KEGGcel:CELE_Y71F9B.10.
UCSCY71F9B.10a.1. c. elegans. [Q9N4G4-1]

Organism-specific databases

CTD171840.
WormBaseY71F9B.10a; CE28808; WBGene00004946; sop-3.
Y71F9B.10b; CE29912; WBGene00004946; sop-3.

Phylogenomic databases

eggNOGNOG270356.
GeneTreeENSGT00750000118762.
HOGENOMHOG000113532.
InParanoidQ9N4G4.
OMAVGLASHQ.
OrthoDBEOG7R830S.

Enzyme and pathway databases

SignaLinkQ9N4G4.

Family and domain databases

InterProIPR019680. Mediator_Med1_met/fun.
[Graphical view]
PfamPF10744. Med1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio872925.
PROQ9N4G4.

Entry information

Entry nameMED1_CAEEL
AccessionPrimary (citable) accession number: Q9N4G4
Secondary accession number(s): Q95Y43, Q9GPV1
Entry history
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: October 1, 2001
Last modified: June 11, 2014
This is version 83 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormBase