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Protein

Serine/threonine-protein kinase chk-1

Gene

chk-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest and activation of DNA repair in response to the presence of DNA damage or unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. Required for checkpoint mediated cell cycle arrest in response to DNA damage in germline cells. Essential for embryogenesis.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei54ATPPROSITE-ProRule annotation1
Active sitei150Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi30 – 38ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • DNA damage checkpoint Source: InterPro
  • embryo development ending in birth or egg hatching Source: UniProtKB
  • intracellular signal transduction Source: GO_Central
  • mitotic DNA replication checkpoint Source: WormBase
  • protein phosphorylation Source: GO_Central

Keywordsi

Molecular functionDevelopmental protein, Kinase, Serine/threonine-protein kinase, Transferase
Biological processCell cycle, DNA damage
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-CEL-176187. Activation of ATR in response to replication stress.
R-CEL-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
SignaLinkiQ9N3Z3.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase chk-1 (EC:2.7.11.1)
Gene namesi
Name:chk-1
ORF Names:Y39H10A.7
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome V

Organism-specific databases

WormBaseiY39H10A.7a; CE26076; WBGene00000498; chk-1.
Y39H10A.7b; CE33867; WBGene00000498; chk-1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000858541 – 503Serine/threonine-protein kinase chk-1Add BLAST503

Proteomic databases

EPDiQ9N3Z3.
PaxDbiQ9N3Z3.
PeptideAtlasiQ9N3Z3.

PTM databases

iPTMnetiQ9N3Z3.

Expressioni

Tissue specificityi

Expressed in the germline.1 Publication

Developmental stagei

Highly expressed in the embryo.1 Publication

Gene expression databases

BgeeiWBGene00000498.

Interactioni

Protein-protein interaction databases

BioGridi533239. 11 interactors.
IntActiQ9N3Z3. 2 interactors.
MINTiMINT-227822.
STRINGi6239.Y39H10A.7a.3.

Structurei

3D structure databases

ProteinModelPortaliQ9N3Z3.
SMRiQ9N3Z3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini24 – 286Protein kinasePROSITE-ProRule annotationAdd BLAST263

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0590. Eukaryota.
ENOG410XQ0D. LUCA.
GeneTreeiENSGT00680000099954.
HOGENOMiHOG000216658.
InParanoidiQ9N3Z3.
KOiK02216.
OMAiAQPADIW.
OrthoDBiEOG091G09E4.
PhylomeDBiQ9N3Z3.

Family and domain databases

CDDicd14069. STKc_Chk1. 1 hit.
InterProiView protein in InterPro
IPR034670. Chk1_catalytic_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
PfamiView protein in Pfam
PF00069. Pkinase. 1 hit.
SMARTiView protein in SMART
SM00220. S_TKc. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform a (identifier: Q9N3Z3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSAASTTSTP AAAAVAPQQP ESLYRVVQTL GEGAFGEVLL IVNTKNPEVA
60 70 80 90 100
AAMKKINIAN KSKDFIDNIR KEYLLQKRVS AVGHDNVIRM IGMRNDPQFY
110 120 130 140 150
YLFLEYADGG ELFDKIEPDC GMSPVFAQFY FKQLICGLKF IHDNDVVHRD
160 170 180 190 200
IKPENLLLTG THVLKISDFG MATLYRNKGE ERLLDLSCGT IPYAAPELCA
210 220 230 240 250
GKKYRGPPVD VWSSGIVLIA MLTGELPWDR ASDASQSYMG WISNTSLDER
260 270 280 290 300
PWKKIDVRAL CMLRKIVTDK TDKRATIEQI QADPWYQHNF GQVETPNGRP
310 320 330 340 350
LKRARNNDEN ITCTQQAECS AKRRHLETPN EKSTLAERQN ASFSQPTKTE
360 370 380 390 400
DLLLTQHIDM SQTNSNLLQR MVCRMTRFCV VTDIRSTYQK VARASEHAGF
410 420 430 440 450
GVRETDDYRL LVTWREVSMM VSLYTMGDIP DKPRVMVDFR RSRGDGIQFK
460 470 480 490 500
KMFMDVRNRM HEWICTDGNN WLANLGYVPR NPQIVNGGGV NVEHSASSIN

VDV
Length:503
Mass (Da):56,935
Last modified:October 1, 2001 - v2
Checksum:iD9634ABE7F433CB7
GO
Isoform b (identifier: Q9N3Z3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-89: Missing.

Note: Gene prediction based on EST data. No experimental confirmation available.
Show »
Length:414
Mass (Da):47,386
Checksum:i57476E54E6E3AE05
GO

Sequence cautioni

Q9N3Z3: The sequence AAA93318 differs from that shown. Reason: Frameshift at position 441.Curated
Q9N3Z3: The sequence CCD73288 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti402V → L in AAA93318 (Ref. 1) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0157711 – 89Missing in isoform b. CuratedAdd BLAST89

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U44902 mRNA. Translation: AAA93318.1. Frameshift.
FO081678 Genomic DNA. Translation: CCD73287.1.
FO081678 Genomic DNA. Translation: CCD73288.1. Different initiation.
RefSeqiNP_001024233.1. NM_001029062.3. [Q9N3Z3-1]
NP_001024234.1. NM_001029063.2.
UniGeneiCel.7221.

Genome annotation databases

EnsemblMetazoaiY39H10A.7a.1; Y39H10A.7a.1; WBGene00000498. [Q9N3Z3-1]
Y39H10A.7a.2; Y39H10A.7a.2; WBGene00000498. [Q9N3Z3-1]
GeneIDi3565921.
KEGGicel:CELE_Y39H10A.7.
UCSCiY39H10A.7a. c. elegans. [Q9N3Z3-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCHK1_CAEEL
AccessioniPrimary (citable) accession number: Q9N3Z3
Secondary accession number(s): Q17375, Q86FM7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 27, 2005
Last sequence update: October 1, 2001
Last modified: October 25, 2017
This is version 129 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families