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Protein

Serine/threonine-protein kinase chk-1

Gene

chk-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest and activation of DNA repair in response to the presence of DNA damage or unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. Required for checkpoint mediated cell cycle arrest in response to DNA damage in germline cells. Essential for embryogenesis.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei54 – 541ATPPROSITE-ProRule annotation
Active sitei150 – 1501Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi30 – 389ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • cellular response to DNA damage stimulus Source: UniProtKB-KW
  • mitotic DNA replication checkpoint Source: WormBase
  • multicellular organism development Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell cycle, DNA damage

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-CEL-176187. Activation of ATR in response to replication stress.
R-CEL-5693616. Presynaptic phase of homologous DNA pairing and strand exchange.
R-CEL-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
SignaLinkiQ9N3Z3.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase chk-1 (EC:2.7.11.1)
Gene namesi
Name:chk-1
ORF Names:Y39H10A.7
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome V

Organism-specific databases

WormBaseiY39H10A.7a; CE26076; WBGene00000498; chk-1.
Y39H10A.7b; CE33867; WBGene00000498; chk-1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 503503Serine/threonine-protein kinase chk-1PRO_0000085854Add
BLAST

Proteomic databases

EPDiQ9N3Z3.
PaxDbiQ9N3Z3.
PeptideAtlasiQ9N3Z3.
PRIDEiQ9N3Z3.

PTM databases

iPTMnetiQ9N3Z3.

Expressioni

Tissue specificityi

Expressed in the germline.1 Publication

Developmental stagei

Highly expressed in the embryo.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi533239. 11 interactions.
IntActiQ9N3Z3. 2 interactions.
MINTiMINT-227822.
STRINGi6239.Y39H10A.7a.3.

Structurei

3D structure databases

ProteinModelPortaliQ9N3Z3.
SMRiQ9N3Z3. Positions 24-339.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini24 – 286263Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0590. Eukaryota.
ENOG410XQ0D. LUCA.
GeneTreeiENSGT00680000099954.
HOGENOMiHOG000216658.
InParanoidiQ9N3Z3.
KOiK02216.
OMAiICSPKAK.
PhylomeDBiQ9N3Z3.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform a (identifier: Q9N3Z3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSAASTTSTP AAAAVAPQQP ESLYRVVQTL GEGAFGEVLL IVNTKNPEVA
60 70 80 90 100
AAMKKINIAN KSKDFIDNIR KEYLLQKRVS AVGHDNVIRM IGMRNDPQFY
110 120 130 140 150
YLFLEYADGG ELFDKIEPDC GMSPVFAQFY FKQLICGLKF IHDNDVVHRD
160 170 180 190 200
IKPENLLLTG THVLKISDFG MATLYRNKGE ERLLDLSCGT IPYAAPELCA
210 220 230 240 250
GKKYRGPPVD VWSSGIVLIA MLTGELPWDR ASDASQSYMG WISNTSLDER
260 270 280 290 300
PWKKIDVRAL CMLRKIVTDK TDKRATIEQI QADPWYQHNF GQVETPNGRP
310 320 330 340 350
LKRARNNDEN ITCTQQAECS AKRRHLETPN EKSTLAERQN ASFSQPTKTE
360 370 380 390 400
DLLLTQHIDM SQTNSNLLQR MVCRMTRFCV VTDIRSTYQK VARASEHAGF
410 420 430 440 450
GVRETDDYRL LVTWREVSMM VSLYTMGDIP DKPRVMVDFR RSRGDGIQFK
460 470 480 490 500
KMFMDVRNRM HEWICTDGNN WLANLGYVPR NPQIVNGGGV NVEHSASSIN

VDV
Length:503
Mass (Da):56,935
Last modified:October 1, 2001 - v2
Checksum:iD9634ABE7F433CB7
GO
Isoform b (identifier: Q9N3Z3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-89: Missing.

Note: Gene prediction based on EST data. No experimental confirmation available.
Show »
Length:414
Mass (Da):47,386
Checksum:i57476E54E6E3AE05
GO

Sequence cautioni

The sequence AAA93318.1 differs from that shown. Reason: Frameshift at position 441. Curated
The sequence CCD73288.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti402 – 4021V → L in AAA93318 (Ref. 1) Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 8989Missing in isoform b. CuratedVSP_015771Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U44902 mRNA. Translation: AAA93318.1. Frameshift.
FO081678 Genomic DNA. Translation: CCD73287.1.
FO081678 Genomic DNA. Translation: CCD73288.1. Different initiation.
RefSeqiNP_001024233.1. NM_001029062.3. [Q9N3Z3-1]
NP_001024234.1. NM_001029063.2.
UniGeneiCel.7221.

Genome annotation databases

EnsemblMetazoaiY39H10A.7a.1; Y39H10A.7a.1; WBGene00000498. [Q9N3Z3-1]
Y39H10A.7a.2; Y39H10A.7a.2; WBGene00000498. [Q9N3Z3-1]
GeneIDi3565921.
KEGGicel:CELE_Y39H10A.7.
UCSCiY39H10A.7a. c. elegans. [Q9N3Z3-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U44902 mRNA. Translation: AAA93318.1. Frameshift.
FO081678 Genomic DNA. Translation: CCD73287.1.
FO081678 Genomic DNA. Translation: CCD73288.1. Different initiation.
RefSeqiNP_001024233.1. NM_001029062.3. [Q9N3Z3-1]
NP_001024234.1. NM_001029063.2.
UniGeneiCel.7221.

3D structure databases

ProteinModelPortaliQ9N3Z3.
SMRiQ9N3Z3. Positions 24-339.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi533239. 11 interactions.
IntActiQ9N3Z3. 2 interactions.
MINTiMINT-227822.
STRINGi6239.Y39H10A.7a.3.

PTM databases

iPTMnetiQ9N3Z3.

Proteomic databases

EPDiQ9N3Z3.
PaxDbiQ9N3Z3.
PeptideAtlasiQ9N3Z3.
PRIDEiQ9N3Z3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiY39H10A.7a.1; Y39H10A.7a.1; WBGene00000498. [Q9N3Z3-1]
Y39H10A.7a.2; Y39H10A.7a.2; WBGene00000498. [Q9N3Z3-1]
GeneIDi3565921.
KEGGicel:CELE_Y39H10A.7.
UCSCiY39H10A.7a. c. elegans. [Q9N3Z3-1]

Organism-specific databases

CTDi3565921.
WormBaseiY39H10A.7a; CE26076; WBGene00000498; chk-1.
Y39H10A.7b; CE33867; WBGene00000498; chk-1.

Phylogenomic databases

eggNOGiKOG0590. Eukaryota.
ENOG410XQ0D. LUCA.
GeneTreeiENSGT00680000099954.
HOGENOMiHOG000216658.
InParanoidiQ9N3Z3.
KOiK02216.
OMAiICSPKAK.
PhylomeDBiQ9N3Z3.

Enzyme and pathway databases

ReactomeiR-CEL-176187. Activation of ATR in response to replication stress.
R-CEL-5693616. Presynaptic phase of homologous DNA pairing and strand exchange.
R-CEL-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
SignaLinkiQ9N3Z3.

Miscellaneous databases

PROiQ9N3Z3.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Winge P., Goebel V., Fleming J.T.
    Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A).
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
    Strain: Bristol N2.
  3. "chk-1 is an essential gene and is required for an S-M checkpoint during early embryogenesis."
    Kalogeropoulos N., Christoforou C., Green A.J., Gill S., Ashcroft N.R.
    Cell Cycle 3:1196-1200(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiCHK1_CAEEL
AccessioniPrimary (citable) accession number: Q9N3Z3
Secondary accession number(s): Q17375, Q86FM7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2005
Last sequence update: October 1, 2001
Last modified: July 6, 2016
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.