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Protein

Mesencephalic astrocyte-derived neurotrophic factor homolog

Gene

manf-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Inhibits endoplasmic reticulum (ER) stress response. Retained in the ER under normal conditions and is up-regulated and secreted by the ER in response to ER stress and hypoxia (By similarity). Following secretion by the ER, directly binds to 3-O-sulfogalactosylceramide, a lipid sulfatide in the outer cell membrane of target cells (PubMed:29497057). Sulfatide binding promotes its cellular uptake by endocytosis, and is required for its role in alleviating ER stress under ER stress conditions (By similarity).By similarity1 Publication

GO - Molecular functioni

  • lipid binding Source: UniProtKB

GO - Biological processi

  • dopaminergic neuron differentiation Source: GO_Central
  • neuron projection development Source: GO_Central
  • regulation of response to endoplasmic reticulum stress Source: UniProtKB

Keywordsi

Biological processStress response
LigandLipid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Mesencephalic astrocyte-derived neurotrophic factor homologImported
Alternative name(s):
ARMET-like protein
MANF/CDNF-like protein
Gene namesi
Name:manf-11 PublicationImported
ORF Names:Y54G2A.23Imported
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome IV

Organism-specific databases

WormBaseiY54G2A.23 ; CE30049 ; WBGene00021888 ; manf-1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum, Secreted

Pathology & Biotechi

Disruption phenotypei

RNAi-mediated knockdown results in up-regulation of ER stress response protein hsp-4 in the intestine and pharyngeal epithelium.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi75S → L in dma1; results in up-regulation of ER stress response protein hsp-4 in the intestine and pharyngeal epithelium. Elevated expression of hsp-4 is suppressed in xbp-1 or ire-1 RNAi-mediated knockdown animals. 1 Publication1
Mutagenesisi146C → G: Does not rescue the elevated expression of the ER stress response protein hsp-4 in the dma1 mutant. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 17Sequence analysisAdd BLAST17
ChainiPRO_000000230718 – 168Mesencephalic astrocyte-derived neurotrophic factor homologAdd BLAST151

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi22 ↔ 109By similarity
Disulfide bondi25 ↔ 97By similarity
Disulfide bondi55 ↔ 66By similarity
Disulfide bondi143 ↔ 146By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

EPDiQ9N3B0
PaxDbiQ9N3B0
PeptideAtlasiQ9N3B0
PRIDEiQ9N3B0

Expressioni

Tissue specificityi

Expressed in the intestine, hypoderm, spermatheca and nervous system.1 Publication

Inductioni

By endoplasmic reticulum stress.1 Publication

Gene expression databases

BgeeiWBGene00021888

Interactioni

Protein-protein interaction databases

STRINGi6239.Y54G2A.23.2

Structurei

3D structure databases

ProteinModelPortaliQ9N3B0
SMRiQ9N3B0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ARMET family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG4154 Eukaryota
ENOG4111QMZ LUCA
GeneTreeiENSGT00390000007160
HOGENOMiHOG000264245
InParanoidiQ9N3B0
KOiK22556
OMAiNRFCYYL
OrthoDBiEOG091G0P3O
PhylomeDBiQ9N3B0

Family and domain databases

Gene3Di1.10.720.30, 1 hit
InterProiView protein in InterPro
IPR019345 Armet_prot
IPR036361 SAP_dom_sf
PANTHERiPTHR12990 PTHR12990, 1 hit
PfamiView protein in Pfam
PF10208 Armet, 1 hit
SUPFAMiSSF68906 SSF68906, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9N3B0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRLVLLISL VIVVASAAAP QCEVCKKVLD DVMAKVPAGD KSKPDAIGKV
60 70 80 90 100
IREHCETTRN KENKFCFYIG ALPESATSIM NEVTKPLSWS MPTEKVCLEK
110 120 130 140 150
LKGKDAQICE LKYDKPLDWK TIDLKKMRVK ELKNILGEWG EVCKGCTEKA
160
ELIKRIEELK PKYVKEEL
Length:168
Mass (Da):18,969
Last modified:January 24, 2001 - v2
Checksum:i5A24379E860A6628
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX284604 Genomic DNA Translation: CCD83510.1
RefSeqiNP_500273.2, NM_067872.5
UniGeneiCel.20205

Genome annotation databases

EnsemblMetazoaiY54G2A.23; Y54G2A.23; WBGene00021888
GeneIDi177074
KEGGicel:CELE_Y54G2A.23
UCSCiY54G2A.23.1 c. elegans

Similar proteinsi

Entry informationi

Entry nameiMANF_CAEEL
AccessioniPrimary (citable) accession number: Q9N3B0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: January 24, 2001
Last modified: May 23, 2018
This is version 94 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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