Reviewed, UniProtKB/Swiss-Prot Q9N337 (MED6_CAEEL)

Names and origin

Protein names

Recommended name:
    Mediator of RNA polymerase II transcription subunit 6
Alternative name(s):
    Mediator complex subunit 6
    CeMED6
    c-MED6
      Short name=MED-6

Gene names

Name:	mdt-6
Synonyms:	med-6
ORF Names:	Y57E12AL.5

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	250 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors By similarity. Acts to repress beta-catenin target genes. Required for asymmetric division of T-cells and for gonad and germ cell development.
Subunit structure	Component of the Mediator complex By similarity. Interacts with let-19/mdt-13. Interacts with RNA polymerase II.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the Mediator complex subunit 6 family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Molecular function	Activator Developmental protein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	embryonic development ending in birth or egg hatching Ref.4 Inferred from mutant phenotype. Source: WormBase gonad development Ref.4 Inferred from mutant phenotype. Source: WormBase growth Inferred from mutant phenotype. Source: WormBase hermaphrodite genitalia development Inferred from mutant phenotype. Source: WormBase negative regulation of vulval development Inferred from mutant phenotype. Source: WormBase nematode larval development Inferred from mutant phenotype. Source: WormBase oogenesis Inferred from mutant phenotype. Source: WormBase regulation of transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: InterPro transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mediator complex Inferred from electronic annotation. Source: InterPro
Molecular function	RNA polymerase II transcription mediator activity Inferred from electronic annotation. Source: InterPro protein binding Ref.3 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ama-1	P16356	1	EBI-1533827,EBI-1533906

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 250	250	Mediator of RNA polymerase II transcription subunit 6		PRO_0000303047
Regions
	Compositional bias	169 – 229	61	Glu-rich

Sequences

	Sequence		Length	Mass (Da)
	Q9N337-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 4AFE17BA9BDE8511	FASTA	250	29,407
	10 20 30 40 50 60 MMPRMGPPAA ARQDNPLHVS FRNPQWPPNF INKDNVLDYF CNQANAFYEM NSCNQQIRMQ 70 80 90 100 110 120 NIVNRTVEEC LRTMPGIQYV LWYSQPPLFI ICKQRRNNVT NVSPIAYYYV INGSVHQAPD 130 140 150 160 170 180 MYSLVQSRLL GALEPLRNAF GEVTNYSRYN TAKGYYWEFK NKPNVKKREE EKKEDEEEKL 190 200 210 220 230 240 EDRSTNFQKT RTMMLLNQLF SEMPAEDALE REEKEEVEEE EEETLKTEEP TTSTDEPKFA 250 EPTARTTSKQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[2]	"A transcriptional mediator protein that is required for activation of many RNA polymerase II promoters and is conserved from yeast to humans." Lee Y.C., Min S., Gim B.S., Kim Y.-J. Mol. Cell. Biol. 17:4622-4632(1997) [PubMed: 9234719] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 4-250.
[3]	"Caenorhabditis elegans mediator complexes are required for developmental-specific transcriptional activation." Kwon J.Y., Park J.M., Gim B.S., Han S.J., Lee J., Kim Y.-J. Proc. Natl. Acad. Sci. U.S.A. 96:14990-14995(1999) [PubMed: 10611325] [Abstract] Cited for: FUNCTION, INTERACTION WITH RNA POLYMERASE II, SUBCELLULAR LOCATION.
[4]	"The MED-7 transcriptional mediator encoded by let-49 is required for gonad and germ cell development in Caenorhabditis elegans." Kwon J.Y., Kim-Ha J., Lee B.J., Lee J. FEBS Lett. 508:305-308(2001) [PubMed: 11728440] [Abstract] Cited for: FUNCTION.
[5]	"Components of the transcriptional Mediator complex are required for asymmetric cell division in C. elegans." Yoda A., Kouike H., Okano H., Sawa H. Development 132:1885-1893(2005) [PubMed: 15790964] [Abstract] Cited for: FUNCTION, INTERACTION WITH LET-19.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC024832 Genomic DNA. Translation: AAF59612.1. U78081 mRNA. Translation: AAB84362.1. Different initiation.
PIR	T37473.
RefSeq	NP_504791.3.
UniGene	Cel.19681
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9N337. 3 interactions.
STRING	Q9N337.
Genome annotation databases
Ensembl	Y57E12AL.5; Y57E12AL.5; Y57E12AL.5; Caenorhabditis elegans. [Genome view]
GeneID	179091.
KEGG	cel:Y57E12AL.5.
NMPDR	fig|6239.3.peg.18332.
UCSC	Y57E12AL.5. c. elegans.
Organism-specific databases
CTD	179091.
WormBase	WBGene00003164. mdt-6.
WormPep	Y57E12AL.5. CE25510. [WorfDB]
Phylogenomic databases
HOGENOM	HBG386155.
InParanoid	Q9N337.
OMA	WWHFKDQ.
Gene expression databases
ArrayExpress	Q9N337.
Family and domain databases
InterPro	IPR007018. Mediator_Med6. IPR016820. Mediator_Med6_met/pln. [Graphical view]
PANTHER	PTHR13104. MED6. 1 hit.
Pfam	PF04934. MED6. 1 hit. [Graphical view]
PIRSF	PIRSF023869. Mediator_MED6_meta/pln. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	903880.

Entry information

Entry name

MED6_CAEEL

Accession

Primary (citable) accession number: Q9N337
Secondary accession number(s): O18534

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	October 1, 2000
Last modified:	December 15, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project