Reviewed, UniProtKB/Swiss-Prot Q9N2J2 (GPX4_BOVIN)

Names and origin

Protein names

Recommended name:
    Phospholipid hydroperoxide glutathione peroxidase, mitochondrial
      Short name=PHGPx
    EC=1.11.1.12
Alternative name(s):
    Glutathione peroxidase 4
      Short name=GPx-4

Gene names

Name:

GPX4

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	197 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility. Could play a major role in protecting mammals from the toxicity of ingested lipid hydroperoxides. Essential for embryonic development. Protects from radiation and oxidative damage By similarity.
Catalytic activity	2 glutathione + a lipid hydroperoxide = glutathione disulfide + lipid + 2 H2O.
Subunit structure	Monomer By similarity.
Subcellular location	Mitochondrion By similarity. Cytoplasm By similarity.
Sequence similarities	Belongs to the glutathione peroxidase family.

Ontologies

Keywords
Cellular component	Cytoplasm Mitochondrion
Coding sequence diversity	Alternative initiation Selenocysteine
Domain	Transit peptide
Ligand	Selenium
Molecular function	Developmental protein Oxidoreductase Peroxidase
Gene Ontology (GO)
Biological process	multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to oxidative stress Inferred from electronic annotation. Source: InterPro
Molecular function	phospholipid-hydroperoxide glutathione peroxidase activity Inferred from electronic annotation. Source: EC selenium binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]

Isoform Mitochondrial (identifier: Q9N2J2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Cytoplasmic (identifier: Q9N2J2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-27: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 197		Phospholipid hydroperoxide glutathione peroxidase, mitochondrial		PRO_0000013065
Sites
	Active site	73	1	By similarity
Amino acid modifications
	Non-standard residue	73	1	Selenocysteine
Natural variations
	Alternative sequence	1 – 27	27	Missing in isoform Cytoplasmic.		VSP_018738

Sequences

	Sequence		Length	Mass (Da)
	Isoform Mitochondrial [UniParc]. Last modified February 26, 2008. Version 2. Checksum: 3C05FF92C4AA8824	FASTA	197	22,240
	10 20 30 40 50 60 MSFSRLYRLL KPALLCGALA APGLASTMCA SRDDWRCARS MHEFSAKDID GRMVNLDKYR 70 80 90 100 110 120 GHVCIVTNVA SQUGKTDVNY TQLVDLHARY AECGLRILAF PCNQFGRQEP GSNAEIKEFA 130 140 150 160 170 180 AGYNVKFDLF SKICVNGDDA HPLWKWMKVQ PKGRGMLGNA IKWNFTKFLI DKNGCVVKRY 190 GPMEEPLVIE KDLPCYL
	Isoform Cytoplasmic. Checksum: 0D89F887D30253BD	FASTA	170	19,436
	10 20 30 40 50 60 MCASRDDWRC ARSMHEFSAK DIDGRMVNLD KYRGHVCIVT NVASQUGKTD VNYTQLVDLH 70 80 90 100 110 120 ARYAECGLRI LAFPCNQFGR QEPGSNAEIK EFAAGYNVKF DLFSKICVNG DDAHPLWKWM 130 140 150 160 170 KVQPKGRGML GNAIKWNFTK FLIDKNGCVV KRYGPMEEPL VIEKDLPCYL

References

[1]	"Bovine phospholipid hydroperoxide glutathione peroxidase." Hara S., Imura N. Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB017534 mRNA. Translation: BAA86034.1.
IPI	IPI00700428. IPI00760481.
RefSeq	NP_777195.1.
UniGene	Bt.49234
3D structure databases
SMR	Q9N2J2. Positions 36-197.
ModBase	Search...
Protein family/group databases
PeroxiBase	3637. BtGPx04-a.
Genome annotation databases
Ensembl	ENSBTAG00000020778. Bos taurus. [Contig view]
GeneID	286809.
KEGG	bta:286809.
Phylogenomic databases
HOVERGEN	Q9N2J2.
Enzyme and pathway databases
BRENDA	1.11.1.12. 251.
Family and domain databases
InterPro	IPR000889. Glutathione_peroxidase. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
PANTHER	PTHR11592. Glut_peroxidase. 1 hit.
Pfam	PF00255. GSHPx. 1 hit. [Graphical view]
PIRSF	PIRSF000303. Glutathion_perox. 1 hit.
PRINTS	PR01011. GLUTPROXDASE.
PROSITE	PS00460. GLUTATHIONE_PEROXID_1. 1 hit. PS00763. GLUTATHIONE_PEROXID_2. 1 hit. PS51355. GLUTATHIONE_PEROXID_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GPX4_BOVIN

Accession

Primary (citable) accession number: Q9N2J2

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	February 26, 2008
Last modified:	June 16, 2009
This is version 53 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)