Q9N2I8 (TRXR2_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thioredoxin reductase 2, mitochondrial
EC=1.8.1.9

Alternative name(s):
Thioredoxin reductase TR3

Gene names

Name:	TXNRD2
Synonyms:	TRXR2

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	511 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Maintains thioredoxin in a reduced state. Implicated in the defenses against oxidative stress. May play a role in redox-regulated cell signaling. Ref.1
Catalytic activity	Thioredoxin + NADP+ = thioredoxin disulfide + NADPH.
Cofactor	FAD. Ref.1
Enzyme regulation	Inhibited by 1-chloro-2,4-dinitrobenzene and by zinc, calcium, magnesium and Fe2+ ions. Ref.1
Subunit structure	Homodimer. Ref.1
Subcellular location	Mitochondrion Ref.1.
Miscellaneous	The active site is a redox-active disulfide bond. The selenocysteine residue is also essential for catalytic activity By similarity. UniProtKB Q9Z0J5
Sequence similarities	Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.

Ontologies

Keywords
Cellular component	Mitochondrion
Coding sequence diversity	Selenocysteine
Domain	Redox-active center Transit peptide
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
PTM	Acetylation Disulfide bond
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro response to oxygen radical Traceable author statement Ref.1. Source: UniProtKB
Cellular component	mitochondrion Inferred from direct assay Ref.1. Source: UniProtKB
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro thioredoxin-disulfide reductase activity Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 21	21	Mitochondrion Ref.1
	Chain	22 – 511	490	Thioredoxin reductase 2, mitochondrial Ref.1		PRO_0000030287
Regions
	Nucleotide binding	29 – 58	30	FAD By similarity
Sites
	Active site	484	1	Proton acceptor By similarity
Amino acid modifications
	Non-standard residue	510	1	Selenocysteine
	Modified residue	140	1	N6-acetyllysine By similarity
	Disulfide bond	74 ↔ 79		Redox-active By similarity UniProtKB P00390
	Cross-link	509 ↔ 510		Cysteinyl-selenocysteine (Cys-Sec) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9N2I8 [UniParc]. Last modified February 26, 2008. Version 2. Checksum: CA74A147B32846ED	FASTA	511	54,670
	10 20 30 40 50 60 MAALRGAAAR FRGRAPGGAR GAAGRQCYDL LVIGGGSGGL ACAKEAAQLG KKVAVLDYVE 70 80 90 100 110 120 PSPQGTRWGL GGTCVNVGCI PKKLMHQAAL LGGMIRDAPH YGWGVAQAPH SWATLADAVQ 130 140 150 160 170 180 NHVKSLNWGH RIQLQDRKVK YFNVKASFVD THTVCGVSKG GEETLLSAEH IVIATGGRPR 190 200 210 220 230 240 YPTHIEGALE YGITSDDLFW LKESPGKTLV VGASYVALEC AGLLTGLGLD TTVMIRSVPL 250 260 270 280 290 300 RAFDQQMASL VTEHMAGHGT RILRGCAPEK VEKLPGQQLR VTWVDLTSDR KDAGTFDTVL 310 320 330 340 350 360 WAIGRVPETA SLNLEKAGVH TNPVTGKILV DAQETTSVPH IYAIGDVAEG RPELTPTAIM 370 380 390 400 410 420 AGRLLAQRLS GRTSDLMDYS SVPTTVFTPL EYGCVGLSEE AAVARHGEEH VEVYHAFYKP 430 440 450 460 470 480 LEFTVPQRDA SQCYIKMVCL REPPQLVLGL HFLGPNAGEV IQGFALGIKC GASYQQLMRT 490 500 510 VGIHPTCAEE VAKLRISKRS GLDPTVTGCU G

References

[1]

"Mitochondrial thioredoxin reductase in bovine adrenal cortex. Its purification, properties, nucleotide/amino acid sequences, and identification of selenocysteine." Watabe S., Makino Y., Ogawa K., Hiroi T., Yamamoto Y., Takahashi S.Y. Eur. J. Biochem. 264:74-84(1999) [PubMed: 10447675] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 22-35; 53-82; 160-181; 208-238; 292-316; 328-340; 420-436; 470-488 AND 499-510, SELENOCYSTEINE AT SEC-510, FUNCTION, SUBCELLULAR LOCATION, ENZYME REGULATION. Tissue: Adrenal cortex.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB022283 mRNA. Translation: BAA82153.1.
IPI	IPI00690111.
RefSeq	NP_777051.1. NM_174626.2.
UniGene	Bt.4008.
3D structure databases
ProteinModelPortal	Q9N2I8.
ModBase	Search...
Proteomic databases
PRIDE	Q9N2I8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	282389.
KEGG	bta:282389.
Organism-specific databases
CTD	10587.
Phylogenomic databases
eggNOG	maNOG16321.
HOVERGEN	HBG004959.
InParanoid	Q9N2I8.
OrthoDB	EOG408N7T.
Family and domain databases
InterPro	IPR016156. FAD/NAD-linked_Rdtase_dimer. IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR004099. Pyr_nucl-diS_OxRdtase_dimer. IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD. IPR012999. Pyr_OxRdtase_I_AS. IPR001327. Pyr_OxRdtase_NAD-bd_dom. IPR006338. Thioredoxin/glutathione_Rdtase. [Graphical view]
Gene3D	G3DSA:3.30.390.30. Pyr_redox_dim. 1 hit.
KO	K00384.
PANTHER	PTHR22912:SF23. Reduct_Se. 1 hit.
Pfam	PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. PF02852. Pyr_redox_dim. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR.
SUPFAM	SSF55424. FAD/NAD-linked_reductase_dimer. 1 hit.
TIGRFAMs	TIGR01438. TGR. 1 hit.
PROSITE	PS00076. PYRIDINE_REDOX_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRXR2_BOVIN

Accession

Primary (citable) accession number: Q9N2I8

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2003
Last sequence update:	February 26, 2008
Last modified:	November 16, 2011
This is version 85 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program